FastQCFastQC Report
Tue 24 May 2016
ERR840912.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840912.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455750
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA650134.465945388974755No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC225341.5479306199553495No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT132140.9077108019921003No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA131530.9035205220676628No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA83860.5760604499398936No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA63840.43853683668212257No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG42090.2891293147861927No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC37700.25897303795294524No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA36550.25107332989867764No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC34690.23829641078481884No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA28740.19742400824317363No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC26960.18519663403743775No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA26910.1848531684698609No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC25590.17578567748583204No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT25510.1752361325777091No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC24540.1685729005667182No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC23110.1587497853340203No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC22920.15744461617722824No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC21750.14940752189592993No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG21710.14913274944186847No Hit
TTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGG20970.14404945904173105No Hit
AGGCTCTCAGTCTCAAACCTCTACAGGCACAACAAGGCTCGACCATGCC20790.1428129829984544No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG20540.14109565516057015No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC20160.1384853168469861No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA20020.1375236132577709No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC19700.13532543362527907No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG19600.13463850249012538No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG19540.13422634380903314No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC19520.1340889575820024No Hit
ATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAATTCC18640.12804396359264983No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC17810.12234243517087412No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG17510.12028164176541302No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT17040.11705306543019062No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT16840.11567920315988323No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA16780.115267044478791No Hit
ACTTCCGACATTTCAAAGCGAGATGGCCGTCTACATTGCCTCGGCGAAGC16530.11354971664090675No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG16020.11004636785162288No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG15970.10970290228404603No Hit
CCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGG15770.10832904001373864No Hit
CAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATA15450.10613086038124679No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT15350.10544392924609308No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC14930.10255881847844753No Hit
AATTCGTGGAGAAAGAAATGGCTCC14800.10166580800274772No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT14750.10132234243517087No Hit
CTGAATACAAGTCTATAAGCTACTTTACTTCAAGTCACATATATATATA14680.10084149064056329No Hit
ATGCAGGCCACAGTGGTAAACACATTCAGTGAGGAGGGCGGCAGAGCAG14650.10063541130001719No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA14570.10008586639189421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG800.096.91421544
TAGTGTA900.096.91421544
GGGATTC201.578788E-596.91421544
GAGATAC1100.096.91421544
ACAGAAT750.096.9142144
GCGTGTA156.112036E-496.9142144
AAGGCTT750.096.9142144
GATGCAG2450.094.9363644
TAAGCCA72150.089.593644
AGACACG1200.088.8380244
TAGTGTT600.088.8380244
ATGAGCC1150.088.48688544
GCCACCT452.1827873E-1186.1459744
CGACGGT408.1308826E-1084.79993444
TAACGAA800.084.79993444
ATGACGC1550.084.4091544
GCTTAGA750.083.9923244
GGATGGC1100.083.6986444
ATAAGTG1000.082.37707544
ATTTAGT1500.080.7618444
Produced by FastQC (version 0.11.5)