##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840912.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1455750
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.72072952086553	38.0	35.0	39.0	30.0	39.0
2	36.43647329555212	39.0	35.0	39.0	32.0	39.0
3	36.714345182895414	39.0	35.0	39.0	33.0	39.0
4	37.06676352395672	39.0	35.0	39.0	33.0	41.0
5	37.70628267216212	39.0	35.0	41.0	33.0	41.0
6	37.73452241112828	39.0	35.0	41.0	33.0	41.0
7	37.7164629915851	39.0	35.0	41.0	33.0	41.0
8	37.529493388287825	39.0	35.0	41.0	33.0	41.0
9	37.55326601408209	39.0	35.0	41.0	33.0	41.0
10	37.5247034174824	39.0	35.0	41.0	33.0	41.0
11	37.495532199896964	39.0	35.0	41.0	33.0	41.0
12	37.68790451657221	39.0	35.0	41.0	33.0	41.0
13	37.7129438433797	39.0	35.0	41.0	33.0	41.0
14	37.77055950540958	39.0	35.0	41.0	33.0	41.0
15	37.70423561737936	39.0	35.0	41.0	33.0	41.0
16	37.72036269963936	39.0	35.0	41.0	33.0	41.0
17	37.72695380388116	39.0	35.0	41.0	33.0	41.0
18	37.751832388803024	39.0	35.0	41.0	33.0	41.0
19	37.71281950884424	39.0	35.0	41.0	33.0	41.0
20	37.64035995191482	39.0	35.0	41.0	33.0	41.0
21	37.51298918083462	39.0	35.0	41.0	33.0	41.0
22	37.483053408895756	39.0	35.0	41.0	33.0	41.0
23	37.39791516400481	39.0	35.0	41.0	33.0	41.0
24	37.30255469689164	39.0	35.0	41.0	33.0	41.0
25	37.20336967843067	39.0	35.0	41.0	32.0	41.0
26	37.22608420655215	39.0	35.0	41.0	32.0	41.0
27	37.15231016827628	39.0	35.0	41.0	32.0	41.0
28	37.06807591032891	39.0	35.0	41.0	32.0	41.0
29	37.05119992255809	39.0	35.0	41.0	32.0	41.0
30	37.06238611129224	38.0	35.0	41.0	32.0	41.0
31	36.96822839531604	38.0	35.0	41.0	32.0	41.0
32	36.92196768955857	38.0	35.0	41.0	32.0	41.0
33	36.86072621603804	38.0	35.0	41.0	32.0	41.0
34	36.80564310281666	38.0	35.0	40.0	32.0	41.0
35	36.69993828857636	38.0	35.0	40.0	31.0	41.0
36	36.715939959156096	38.0	35.0	40.0	31.0	41.0
37	36.667261876328325	38.0	35.0	40.0	31.0	41.0
38	36.554113326072866	38.0	35.0	40.0	31.0	41.0
39	36.41199358079175	37.0	34.0	40.0	31.0	41.0
40	36.37616867655324	37.0	34.0	40.0	31.0	41.0
41	36.27473594080123	37.0	34.0	40.0	31.0	41.0
42	36.16390507778568	37.0	34.0	40.0	31.0	41.0
43	36.11295250882143	37.0	34.0	40.0	31.0	41.0
44	36.04489951829901	36.0	34.0	40.0	31.0	41.0
45	35.7988614800759	36.0	34.0	40.0	30.0	41.0
46	35.65083877160413	36.0	34.0	39.0	30.0	41.0
47	35.58510572389219	36.0	34.0	39.0	30.0	41.0
48	35.50915237538529	36.0	34.0	39.0	30.0	41.0
49	35.84930451829724	36.0	34.0	39.0	30.0	41.0
50	36.21490654626958	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	307.0
15	1701.0
16	672.0
17	4.0
18	0.0
19	0.0
20	6.0
21	19.0
22	66.0
23	190.0
24	544.0
25	1128.0
26	2508.0
27	4777.0
28	8933.0
29	15910.0
30	26829.0
31	42946.0
32	65066.0
33	100556.0
34	166384.0
35	94377.0
36	119251.0
37	150820.0
38	177408.0
39	236751.0
40	238594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.06766271681264	22.126532715095312	33.80580456809204
2	21.998900910183757	28.840185471406492	28.439567233384857	20.7213463850249
3	26.87563111798042	17.558715438777263	23.138176197836167	32.42747724540615
4	19.024145629400653	21.20728147003263	28.647089129314786	31.121483771251935
5	17.682706508672506	23.406422806113685	31.384647089129313	27.526223596084492
6	25.439395500601066	23.46900223252619	24.101734501116262	26.989867765756482
7	27.353048256912242	21.808277520178603	22.705272196462303	28.133402026446845
8	20.15874978533402	24.801992100291944	27.67879100120213	27.360467113171904
9	21.63633865704963	25.83142709943328	29.325090159711486	23.2071440838056
10	29.175133092907434	22.33906920831187	20.287480680061822	28.19831701871887
11	22.96362699639361	26.803365962562253	22.197492701356687	28.035514339687445
12	21.4163146144599	22.20724712347587	27.3801133436373	28.99632491842693
13	18.60889575820024	24.128868280954833	24.532852481538725	32.7293834793062
14	20.099879787051346	24.170496307745147	21.68071440838056	34.048909496822944
15	21.104997424008243	27.07964966512107	29.266838399450457	22.54851451142023
16	26.301425382105442	31.51935428473295	19.030946247638674	23.148274085522928
17	23.386845268761807	29.32989867765756	18.407693628713723	28.875562424866906
18	22.873776403915507	24.089232354456467	20.608140133951572	32.42885110767645
19	22.20772797527048	29.76074188562597	21.44468487034175	26.586845268761806
20	29.423664777606046	21.707161257083975	24.06127425725571	24.80789970805427
21	23.509668555727288	28.638021638330756	24.403503348789286	23.44880645715267
22	24.54597286622016	30.383376266529282	20.79498540271338	24.27566546453718
23	30.542332131203846	24.769362871372145	21.29184269277005	23.39646230465396
24	21.91111111111111	25.660930791688134	22.091430534088957	30.336527563111797
25	22.96087394329888	30.039136437719083	20.158275920536767	26.841713698445268
26	23.35995887402314	27.69824789685627	27.305238477029288	21.636554752091303
27	23.573973231260968	28.714797452493833	20.76611799965483	26.94511131659036
28	22.28512591181297	24.158927180754155	26.33929265503824	27.216654252394633
29	29.074170560115707	24.005996053879073	24.047777673383045	22.872055712622178
30	24.692827420853842	22.818442321348343	23.836964754423164	28.651765503374655
31	22.493966524783325	24.59019711159345	26.804459025493465	26.111377338129753
32	20.614959466195597	26.190288794246854	27.744886269228413	25.449865470329136
33	24.227285952629117	23.557419977386314	26.25758239815555	25.95771167182902
34	22.391614842807968	24.295613276597763	28.749301063866078	24.56347081672819
35	21.158949501228253	23.854154837398617	22.84928964622768	32.13760601514545
36	19.51530593049312	24.227931272677473	29.97038290033876	26.286379896490647
37	18.747057124204712	26.006567778457658	23.59289782183593	31.6534772755017
38	24.70268685821957	29.553374013388755	20.73695545526278	25.00698367312889
39	20.640223437825494	31.298578052788727	24.047110198980025	24.014088310405754
40	19.91540674647133	31.531626076364027	22.53529447889483	26.017672698269813
41	26.748963778668923	25.10479720927245	21.753088296006386	26.393150716052244
42	19.624338624338623	30.456289978678036	25.27821211403301	24.64115928295033
43	21.53519532963992	24.160401481506256	26.794048810709786	27.510354378144044
44	20.61712410837066	26.747593505480456	25.463968926667256	27.171313459481627
45	20.403437004496894	29.41330807621325	27.13041966676198	23.052835252527878
46	19.342410951654244	32.84641600080207	24.365343319864007	23.44582972767967
47	25.802557198735464	24.977060226115842	23.12521767668649	26.095164898462198
48	21.137971646976585	26.87873904845824	20.602861667565744	31.380427636999432
49	20.61117966035653	23.88371838166055	20.56744569276228	34.93765626522064
50	17.449151123248143	39.1948552143446	20.601201823990106	22.754791838417148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4167.0
1	3232.0
2	1706.5
3	927.5
4	700.0
5	619.0
6	538.0
7	639.5
8	997.0
9	1439.5
10	1791.0
11	2086.0
12	2372.0
13	3247.0
14	4198.0
15	3944.0
16	3391.0
17	4359.5
18	5395.5
19	4758.0
20	4121.0
21	5288.5
22	6541.5
23	5705.0
24	4792.0
25	7606.0
26	12309.0
27	13046.5
28	9790.5
29	9725.5
30	14141.5
31	24805.5
32	33807.5
33	30836.5
34	29315.0
35	44064.5
36	57597.0
37	51883.0
38	47143.5
39	55218.0
40	62336.5
41	71760.0
42	86947.0
43	124447.5
44	152351.5
45	118597.0
46	91960.5
47	93480.5
48	90500.5
49	99020.5
50	120479.0
51	129881.5
52	116844.0
53	102323.5
54	103205.5
55	98829.0
56	81421.5
57	72355.0
58	71414.5
59	71496.0
60	67033.5
61	61310.5
62	58524.0
63	55214.0
64	51575.0
65	48577.5
66	43848.5
67	38845.5
68	32963.0
69	26753.0
70	23725.0
71	24041.0
72	25105.0
73	24603.5
74	19354.0
75	13904.0
76	12485.0
77	10514.5
78	8240.0
79	6839.5
80	5817.5
81	4699.0
82	4104.5
83	4074.0
84	3840.0
85	3432.5
86	3278.0
87	2624.0
88	1789.5
89	1491.5
90	819.0
91	233.5
92	164.5
93	157.0
94	83.5
95	8.0
96	6.0
97	6.0
98	116.0
99	226.0
100	226.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10805.0
25	9356.0
26	10195.0
27	10356.0
28	10114.0
29	9373.0
30	12020.0
31	11343.0
32	14603.0
33	9107.0
34	9990.0
35	9526.0
36	10099.0
37	12244.0
38	10509.0
39	9719.0
40	9878.0
41	10213.0
42	10551.0
43	12239.0
44	12438.0
45	14233.0
46	22407.0
47	41398.0
48	167530.0
49	364448.0
50	621056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.050590982955109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.37443486536517	6.972540695054458
2	13.175184708948148	2.648367852690446
3	5.3693693913894105	1.618960067677607
4	2.7147003179078535	1.0913737014636016
5	1.6567993261088492	0.832590618377785
6	1.0840668048637714	0.6537307232330866
7	0.7869466831833616	0.5536495466648387
8	0.5718494554059171	0.45979399840884
9	0.4751509089432455	0.4297992696871117
>10	3.080229292577564	6.267101761471381
>50	0.4613043392899331	3.3036448615871157
>100	0.6663284900854589	16.270881955053376
>500	0.4722389606656945	34.97603577518456
>1k	0.10729597224965852	15.082594616852301
>5k	0.0013668276719701721	1.0145089008803396
>10k+	0.0027336553439403443	7.824425655713135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	65013	4.465945388974755	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	22534	1.5479306199553495	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	13214	0.9077108019921003	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	13153	0.9035205220676628	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	8386	0.5760604499398936	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	6384	0.43853683668212257	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	4209	0.2891293147861927	No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC	3770	0.25897303795294524	No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA	3655	0.25107332989867764	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	3469	0.23829641078481884	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	2874	0.19742400824317363	No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	2696	0.18519663403743775	No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA	2691	0.1848531684698609	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	2559	0.17578567748583204	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	2551	0.1752361325777091	No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC	2454	0.1685729005667182	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	2311	0.1587497853340203	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	2292	0.15744461617722824	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	2175	0.14940752189592993	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	2171	0.14913274944186847	No Hit
TTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGG	2097	0.14404945904173105	No Hit
AGGCTCTCAGTCTCAAACCTCTACAGGCACAACAAGGCTCGACCATGCC	2079	0.1428129829984544	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2054	0.14109565516057015	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	2016	0.1384853168469861	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2002	0.1375236132577709	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1970	0.13532543362527907	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	1960	0.13463850249012538	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	1954	0.13422634380903314	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1952	0.1340889575820024	No Hit
ATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAATTCC	1864	0.12804396359264983	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	1781	0.12234243517087412	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1751	0.12028164176541302	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	1704	0.11705306543019062	No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT	1684	0.11567920315988323	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1678	0.115267044478791	No Hit
ACTTCCGACATTTCAAAGCGAGATGGCCGTCTACATTGCCTCGGCGAAGC	1653	0.11354971664090675	No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG	1602	0.11004636785162288	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	1597	0.10970290228404603	No Hit
CCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGG	1577	0.10832904001373864	No Hit
CAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATA	1545	0.10613086038124679	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	1535	0.10544392924609308	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	1493	0.10255881847844753	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	1480	0.10166580800274772	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT	1475	0.10132234243517087	No Hit
CTGAATACAAGTCTATAAGCTACTTTACTTCAAGTCACATATATATATA	1468	0.10084149064056329	No Hit
ATGCAGGCCACAGTGGTAAACACATTCAGTGAGGAGGGCGGCAGAGCAG	1465	0.10063541130001719	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	1457	0.10008586639189421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05323716297441181	0.0	0.0	0.0	0.0
2	0.09204877211059591	0.0	0.0	0.0	0.0
3	0.12275459385196634	0.0	0.0	0.0	0.0
4	0.15909325090159712	0.0	0.0	0.0	0.0
5	0.20449939893525673	0.0	0.0	0.0	0.0
6	0.2665979735531513	0.0	0.0	0.0	0.0
7	0.3281470032629229	0.0	0.0	0.0	0.0
8	0.41614288167611196	0.0	0.0	0.0	0.0
9	0.4916366134295037	0.0	0.0	0.0	0.0
10	0.5647947793233729	0.0	0.0	0.0	0.0
11	0.6311523269792203	0.0	0.0	0.0	0.0
12	0.7008758371973209	0.0	0.0	0.0	0.0
13	0.7740340030911901	0.0	0.0	0.0	0.0
14	0.8531684698608965	0.0	0.0	0.0	0.0
15	0.9477588871715611	0.0	0.0	0.0	0.0
16	1.0319079512278895	0.0	0.0	0.0	0.0
17	1.1157135497166408	0.0	0.0	0.0	0.0
18	1.1858492186158338	0.0	0.0	0.0	0.0
19	1.2830499742400825	0.0	0.0	0.0	0.0
20	1.3745492014425553	0.0	0.0	0.0	0.0
21	1.4534775888717155	0.0	0.0	0.0	0.0
22	1.520934226343809	0.0	0.0	0.0	0.0
23	1.581246780010304	0.0	0.0	0.0	0.0
24	1.6434140477417138	0.0	0.0	0.0	0.0
25	1.7151983513652755	0.0	0.0	0.0	0.0
26	1.792684183410613	0.0	0.0	0.0	0.0
27	1.8652928043963592	0.0	0.0	0.0	0.0
28	1.928902627511592	0.0	0.0	0.0	0.0
29	2.019371458011334	0.0	0.0	0.0	0.0
30	2.076318049115576	0.0	0.0	0.0	0.0
31	2.1413017345011163	0.0	0.0	0.0	0.0
32	2.2053924094109565	0.0	0.0	0.0	0.0
33	2.2684526876180664	0.0	0.0	0.0	0.0
34	2.3387944358578054	0.0	0.0	0.0	0.0
35	2.4010303967027307	0.0	0.0	0.0	0.0
36	2.472746007212777	0.0	0.0	0.0	0.0
37	2.536561909668556	0.0	0.0	0.0	0.0
38	2.603606388459557	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	80	0.0	96.914215	44
TAGTGTA	90	0.0	96.914215	44
GGGATTC	20	1.578788E-5	96.914215	44
GAGATAC	110	0.0	96.914215	44
ACAGAAT	75	0.0	96.91421	44
GCGTGTA	15	6.112036E-4	96.91421	44
AAGGCTT	75	0.0	96.91421	44
GATGCAG	245	0.0	94.93636	44
TAAGCCA	7215	0.0	89.5936	44
AGACACG	120	0.0	88.83802	44
TAGTGTT	60	0.0	88.83802	44
ATGAGCC	115	0.0	88.486885	44
GCCACCT	45	2.1827873E-11	86.14597	44
CGACGGT	40	8.1308826E-10	84.799934	44
TAACGAA	80	0.0	84.799934	44
ATGACGC	155	0.0	84.40915	44
GCTTAGA	75	0.0	83.99232	44
GGATGGC	110	0.0	83.69864	44
ATAAGTG	100	0.0	82.377075	44
ATTTAGT	150	0.0	80.76184	44
>>END_MODULE