FastQCFastQC Report
Tue 24 May 2016
ERR840911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9257408
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA4109854.439525620994559No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC691250.7466992920696592No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT302270.3265168824794154No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA274160.29615201144856096No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT250660.27076693605812774No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT235200.2540667970991448No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG231240.2497891418418633No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA215590.2328837618478088No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC207310.22393957358258382No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG196090.21181955035361952No Hit
ACCGCGGCCTCAGATGAATGCGGCTG191340.2066885244768298No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA144930.15655570112065928No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT140060.15129504932698223No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA134130.14488936860080057No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGG129810.1402228355928571No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG124770.1347785470835897No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT119420.1289993916223634No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG114390.12356590527283662No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT112940.1219995921104482No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG109360.11813241892330985No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG107700.1163392604063686No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA101960.11013882071525853No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG96230.10394918318388906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA467250.0108.930344
TATCGAT1000.079.43896544
GGAGACA52900.065.75220544
TTAAGCC469800.065.1544543
CTATCAT57000.064.5068344
AATCGCA8200.063.6618944
CGGTACG904.0017767E-1156.7421244
CGAAGTA1001.8189894E-1256.7421244
ATTAAGC468500.056.44403542
GAACACA59900.054.75282744
CGTAGAT2100.054.04011544
GATTAAG484200.053.74990541
AGATTAA498850.051.60005640
ATAACGT1300.050.7935738
GAGTAAC3700.050.60783444
CAAAGAT499300.049.34797337
GGCGGTA1500.049.17650644
CGAATAT900.049.04749737
ACATTGG19450.049.01118544
AAAGATT510200.049.00992238
Produced by FastQC (version 0.11.5)