##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840911.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9257408
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.14356977676689	35.0	32.0	38.0	27.0	39.0
2	35.898844903454616	37.0	35.0	39.0	32.0	39.0
3	35.509234982405445	37.0	34.0	39.0	30.0	39.0
4	35.87347689547657	37.0	34.0	39.0	31.0	39.0
5	36.11809158675949	38.0	34.0	39.0	31.0	40.0
6	35.88424589258678	37.0	34.0	39.0	30.0	40.0
7	35.824660315284795	37.0	34.0	39.0	30.0	40.0
8	35.39762879631102	37.0	33.0	38.0	30.0	40.0
9	35.44085547488022	37.0	33.0	39.0	30.0	40.0
10	35.355851767579004	37.0	33.0	39.0	30.0	40.0
11	35.380873026229374	37.0	33.0	39.0	30.0	40.0
12	35.98341598425823	37.0	34.0	39.0	30.0	40.0
13	36.377360920032906	38.0	35.0	39.0	31.0	40.0
14	36.51914520781627	38.0	35.0	39.0	31.0	40.0
15	36.48442685036675	38.0	35.0	40.0	31.0	40.0
16	36.37930973767171	38.0	35.0	40.0	31.0	40.0
17	36.60352897916998	38.0	35.0	40.0	31.0	40.0
18	36.734363225645886	38.0	35.0	40.0	31.0	40.0
19	36.5387958486868	38.0	35.0	40.0	31.0	40.0
20	36.57056986145582	38.0	35.0	40.0	31.0	40.0
21	36.352752735971016	38.0	35.0	40.0	30.0	40.0
22	36.27706243475495	38.0	35.0	40.0	30.0	40.0
23	36.22929668866274	38.0	34.0	40.0	30.0	40.0
24	36.0567696703008	38.0	34.0	40.0	30.0	40.0
25	36.08824442488363	38.0	34.0	40.0	30.0	40.0
26	35.92670187909094	38.0	34.0	40.0	30.0	40.0
27	35.900458054828526	38.0	34.0	40.0	30.0	40.0
28	35.92048074660361	38.0	34.0	40.0	30.0	40.0
29	35.89969247994673	38.0	34.0	40.0	30.0	40.0
30	35.92960356734516	38.0	34.0	40.0	30.0	40.0
31	35.79238838888006	38.0	34.0	40.0	29.0	40.0
32	35.77980689779462	38.0	33.0	40.0	29.0	40.0
33	35.74262533097597	37.0	33.0	40.0	29.0	40.0
34	35.66765415849288	37.0	33.0	39.0	29.0	40.0
35	35.603384008949135	37.0	33.0	40.0	29.0	40.0
36	35.67815114957223	37.0	33.0	40.0	29.0	40.0
37	35.60937727040958	37.0	33.0	40.0	29.0	40.0
38	35.482603951734	37.0	33.0	40.0	28.0	40.0
39	35.55868481399111	37.0	33.0	40.0	29.0	40.0
40	35.44167568500654	37.0	33.0	40.0	28.0	40.0
41	35.473834703587634	37.0	33.0	40.0	28.0	40.0
42	35.326732041303764	37.0	33.0	39.0	28.0	40.0
43	35.39643030809808	37.0	33.0	39.0	29.0	40.0
44	35.281594033997266	37.0	33.0	39.0	28.0	40.0
45	35.02100072567168	36.0	33.0	39.0	28.0	40.0
46	35.07089696331403	36.0	33.0	39.0	28.0	40.0
47	34.85220106453695	36.0	33.0	39.0	28.0	40.0
48	34.799176287711305	36.0	32.0	39.0	28.0	40.0
49	35.02139172264453	36.0	33.0	39.0	28.0	40.0
50	35.075659708696115	36.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	356.0
15	5311.0
16	6766.0
17	168.0
18	0.0
19	9.0
20	31.0
21	145.0
22	441.0
23	1203.0
24	2909.0
25	6888.0
26	14292.0
27	28858.0
28	58139.0
29	118114.0
30	229979.0
31	412484.0
32	646889.0
33	892208.0
34	1105273.0
35	1239361.0
36	1306122.0
37	1288741.0
38	1124359.0
39	662910.0
40	105449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.34896722711152	21.365883409265315	32.28514936362317
2	23.227441201684098	25.58186913658769	28.53533083990681	22.6553588218214
3	18.481361089410772	18.144733385414145	30.340987455667936	33.03291806950715
4	18.14774718798178	19.69950984119961	33.00888326408429	29.143859706734325
5	18.178533343242513	20.981693795930784	34.57473193360387	26.265040927222827
6	24.80872615747302	23.96338154265211	27.646086247900058	23.58180605197481
7	30.29585603227167	21.983442881635984	21.908346267119263	25.812354818973088
8	21.353504134202574	20.688469169771928	31.549803141440886	26.40822355458461
9	22.300648302418992	21.017870228902087	32.61991909614441	24.061562372534514
10	28.46900557909946	18.360819788865307	23.854214916313506	29.315959715721725
11	22.835592857093477	26.216312384632936	24.819625536651298	26.128469221622296
12	25.818155578753792	20.022710460638656	28.62097036233036	25.538163598277187
13	23.363494403617082	19.193028977441635	25.93023878822236	31.51323783071892
14	24.243654379281978	21.50571736710751	22.637059963220807	31.61356829038971
15	22.06933085373357	24.118262909012977	28.197925380408854	25.614480856844597
16	27.859828582687506	25.868299204269707	22.66581531245031	23.606056900592478
17	24.17321349561346	25.06837767115806	19.85641121143197	30.90199762179651
18	24.167455944471712	21.610066230201802	21.01900445567485	33.20347336965164
19	24.73609243537716	26.95534214328676	22.45038784074333	25.858177580592756
20	31.384152021818636	20.097526219002123	22.750223388663436	25.76809837051581
21	25.714757305716674	24.077819622944133	25.184522492689098	25.02290057865009
22	25.948624064100876	29.288705866696162	20.450011493497964	24.312658575704994
23	32.289373008081746	22.636509053074036	21.75068874570506	23.32342919313916
24	26.880105100693413	22.673333615629772	21.23256315374671	29.213998129930108
25	26.591299154733612	28.9344156141091	22.543416114924035	21.930869116233257
26	25.679469392795585	23.757161884393632	27.25558783713736	23.307780885673427
27	25.526598976844923	28.395280496346764	21.990467752283767	24.08765277452455
28	26.07369611258746	21.690682951747416	27.375258998214992	24.860361937450136
29	31.871962164004785	22.614370459421764	23.581161837761663	21.932505538811785
30	27.829332061781887	22.42212811732333	20.89062528826327	28.85791453263152
31	23.912998463857306	23.58782017017957	28.884538892339997	23.614642473623128
32	23.282722799618355	22.71136936645968	29.88133033146384	24.124577502458127
33	29.222289103627354	21.393672897572536	25.818399753858756	23.565638244941354
34	25.163448443953673	22.75205616491563	29.354266798976376	22.730228592154322
35	22.263502012747466	22.995148112050693	24.009843429912838	30.731506445289003
36	22.627143975258328	22.82793050960698	30.76876910811358	23.77615640702111
37	20.926736607390538	24.610764284261226	23.570503895527477	30.89199521282076
38	22.415717329943686	29.0628361065153	23.85172130802293	24.66972525551808
39	23.915952539204813	29.785366341024456	23.75334695140385	22.545334168366885
40	21.223528682746977	31.294566090599456	23.09451607477587	24.387389151877695
41	29.781622214123548	23.48997339978987	22.577875947671092	24.15052843841549
42	21.167679276369217	31.12465499938166	23.56216735899498	24.14549836525414
43	23.11112274284346	22.273869363082163	29.54931551673256	25.065692377341815
44	23.607515774356077	25.001662781937185	28.733416325281347	22.657405118425388
45	24.36365875779932	28.900133162176907	25.181511569765846	21.554696510257923
46	22.60549030382123	30.243362257786327	22.123695919286277	25.027451519106165
47	28.505506727401002	24.395246734663097	22.73979668306112	24.35944985487478
48	22.942943610910707	23.279204681801154	21.92888969695563	31.84896201033251
49	20.65560596581011	21.899944178240002	23.41335076670625	34.03109908924363
50	18.699061028113505	39.560925666412025	21.358827904058927	20.381185401415543
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33587.0
1	25120.0
2	13879.5
3	9408.5
4	7299.5
5	6306.5
6	5068.5
7	4610.5
8	5175.5
9	6164.5
10	6999.0
11	8047.0
12	9306.0
13	10406.0
14	11658.0
15	14493.5
16	17251.5
17	19434.5
18	20670.5
19	20586.5
20	21603.0
21	27505.0
22	33311.0
23	40459.5
24	46689.0
25	54578.0
26	65219.0
27	71152.5
28	76886.5
29	92704.5
30	109682.5
31	135455.0
32	173208.0
33	192281.5
34	210191.5
35	262855.0
36	298016.0
37	292187.0
38	297213.5
39	327280.0
40	360222.5
41	367854.5
42	376471.5
43	615906.5
44	874898.0
45	720414.0
46	538694.5
47	521881.0
48	535808.5
49	612462.5
50	697931.0
51	730890.5
52	732140.5
53	745409.5
54	728578.0
55	682358.0
56	650153.0
57	655505.0
58	652486.0
59	610245.0
60	568083.0
61	541725.5
62	515262.0
63	493005.5
64	458870.0
65	422243.0
66	390921.0
67	346163.0
68	303672.0
69	269664.0
70	232711.5
71	199444.5
72	171388.0
73	149734.5
74	139429.0
75	128386.0
76	118145.0
77	101140.0
78	77582.0
79	63230.0
80	53276.0
81	42230.0
82	33825.0
83	31681.0
84	30197.0
85	24321.0
86	17802.0
87	11556.0
88	5828.0
89	3477.5
90	2178.0
91	1229.5
92	922.0
93	792.5
94	467.0
95	111.0
96	66.0
97	66.0
98	33.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	138271.0
25	138784.0
26	142983.0
27	125920.0
28	133798.0
29	121840.0
30	149292.0
31	138721.0
32	120387.0
33	112494.0
34	129915.0
35	123711.0
36	111072.0
37	115366.0
38	108243.0
39	116899.0
40	106460.0
41	104424.0
42	102881.0
43	120107.0
44	122023.0
45	122267.0
46	126409.0
47	221033.0
48	865688.0
49	2175812.0
50	3162608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.30717259989446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.38000596071777	5.514301666977056
2	12.07988742880951	2.0069941971683183
3	5.171015758440575	1.2886956126642004
4	2.9014246361496436	0.9641054095232431
5	1.8340952273963183	0.7618072809311948
6	1.3765958222320451	0.6861371457345138
7	1.0180726374052929	0.5920113582708876
8	0.8059782049281646	0.5356320048073098
9	0.6460513127715158	0.4830173787223244
>10	5.181140826645831	8.878003570098969
>50	0.8548053635954894	5.018124113599858
>100	1.209355929524246	24.47932264356964
>500	0.4567355948652087	25.797534942418626
>1k	0.07666308905570951	11.018093789199558
>5k	0.005318420729753113	3.092133934106043
>10k+	0.0028537867330382556	8.88408495220836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	410985	4.439525620994559	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	69125	0.7466992920696592	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	30227	0.3265168824794154	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	27416	0.29615201144856096	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	25066	0.27076693605812774	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	23520	0.2540667970991448	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	23124	0.2497891418418633	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	21559	0.2328837618478088	No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC	20731	0.22393957358258382	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	19609	0.21181955035361952	No Hit
ACCGCGGCCTCAGATGAATGCGGCTG	19134	0.2066885244768298	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	14493	0.15655570112065928	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	14006	0.15129504932698223	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	13413	0.14488936860080057	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGG	12981	0.1402228355928571	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	12477	0.1347785470835897	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT	11942	0.1289993916223634	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	11439	0.12356590527283662	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT	11294	0.1219995921104482	No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG	10936	0.11813241892330985	No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG	10770	0.1163392604063686	No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA	10196	0.11013882071525853	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	9623	0.10394918318388906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.027210640386596336	0.0	0.0	0.0	0.0
2	0.04985196720291468	0.0	0.0	0.0	0.0
3	0.06266332865527802	0.0	0.0	0.0	0.0
4	0.08270133497410938	0.0	0.0	0.0	0.0
5	0.10730865486321874	0.0	0.0	0.0	0.0
6	0.1432906489591903	0.0	0.0	0.0	0.0
7	0.1774254737395176	0.0	0.0	0.0	0.0
8	0.22169272435653695	0.0	0.0	0.0	0.0
9	0.2612718376461316	0.0	0.0	0.0	0.0
10	0.3004296667058425	0.0	0.0	0.0	0.0
11	0.34383274454361307	0.0	0.0	0.0	0.0
12	0.3839843722994601	0.0	0.0	0.0	0.0
13	0.42658809031642553	0.0	0.0	0.0	0.0
14	0.471017373329554	0.0	0.0	0.0	0.0
15	0.5220899845831576	0.0	0.0	0.0	0.0
16	0.5666812999923952	0.0	0.0	0.0	0.0
17	0.6205840770980386	0.0	0.0	0.0	0.0
18	0.6612758128409162	0.0	0.0	0.0	0.0
19	0.713893132937427	0.0	0.0	0.0	0.0
20	0.7597591031960567	0.0	0.0	0.0	0.0
21	0.8023196125740596	0.0	0.0	0.0	0.0
22	0.8502812018223675	0.0	0.0	0.0	0.0
23	0.8873758183716219	0.0	0.0	0.0	0.0
24	0.9276246655651345	0.0	0.0	0.0	0.0
25	0.9746140604367874	0.0	0.0	0.0	0.0
26	1.0167856920641285	0.0	0.0	0.0	0.0
27	1.0623600040097616	0.0	0.0	0.0	0.0
28	1.0992277752044632	0.0	0.0	0.0	0.0
29	1.1554638188140784	0.0	0.0	0.0	0.0
30	1.1942867809218305	0.0	0.0	0.0	0.0
31	1.235583437610182	0.0	0.0	0.0	0.0
32	1.2742767738010468	0.0	0.0	0.0	0.0
33	1.312289573928253	0.0	0.0	0.0	0.0
34	1.3522251584892877	0.0	0.0	0.0	0.0
35	1.3924091927243565	0.0	0.0	0.0	0.0
36	1.4381023284271364	0.0	0.0	0.0	0.0
37	1.4759747004777146	0.0	0.0	0.0	0.0
38	1.5127236479152697	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	46725	0.0	108.9303	44
TATCGAT	100	0.0	79.438965	44
GGAGACA	5290	0.0	65.752205	44
TTAAGCC	46980	0.0	65.15445	43
CTATCAT	5700	0.0	64.50683	44
AATCGCA	820	0.0	63.66189	44
CGGTACG	90	4.0017767E-11	56.74212	44
CGAAGTA	100	1.8189894E-12	56.74212	44
ATTAAGC	46850	0.0	56.444035	42
GAACACA	5990	0.0	54.752827	44
CGTAGAT	210	0.0	54.040115	44
GATTAAG	48420	0.0	53.749905	41
AGATTAA	49885	0.0	51.600056	40
ATAACGT	130	0.0	50.79357	38
GAGTAAC	370	0.0	50.607834	44
CAAAGAT	49930	0.0	49.347973	37
GGCGGTA	150	0.0	49.176506	44
CGAATAT	90	0.0	49.047497	37
ACATTGG	1945	0.0	49.011185	44
AAAGATT	51020	0.0	49.009922	38
>>END_MODULE