FastQCFastQC Report
Tue 24 May 2016
ERR840910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081041
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC71480.34348194004827387No Hit
AATTCGTGGAGAAAGAAATGGCTCC62030.2980719745550424No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG42250.20302339069725198No Hit
ATCAAGGGAAAGGCAAGACTGGGACGTCTAGTCCACAGGAAGACCTGCAC36480.175296882665935No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA33670.16179402520181005No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT25710.1235439378657124No Hit
CAGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGAT24520.11782564591471288No Hit
AACAAGAGCTGGAAGGAATGTCTCCAGATGCTAATAAGCTTGCAGGACTT23440.11263593557262928No Hit
TACCACACTACCCTGTTGACACAGCCTAGATCCAGAGTTCAGAAGACCT23370.1122995654578646No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA23240.11167487810187304No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT22980.11042550338988998No Hit
ACATCAGAAATCTTTAGTTCCTGATGAATTAAAAAAAGAGGTACTAGTTC21440.10302536086506706No Hit
AGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCG21160.10167988040600834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAC150.002262194669.70750444
ACAAGCG150.002262194669.70750444
TCGCATC252.9126422E-669.707544
ACCGACT1150.069.707544
ATAGACC550.069.707544
CGATTAT550.069.707544
CCGCGTA750.069.707544
CAATCGT750.069.707544
TATCGTC252.9126422E-669.707544
TACGGAT750.065.0603344
TTGCGTG650.064.3453844
CGGCACA1600.063.1724244
ATCGGGA900.061.9622244
TCTACGG1300.061.66432644
GCCCGAC850.061.50661544
TTCCGTT1100.060.20192744
GACGTGT308.590992E-658.08958444
ATAGAAC1200.058.08958444
TACTGCG800.056.63734444
AACCTAT1000.055.76644
Produced by FastQC (version 0.11.5)