##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840910.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2081041
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.07937854179711	38.0	35.0	39.0	31.0	39.0
2	36.734054735106135	39.0	37.0	39.0	32.0	39.0
3	36.84323566907139	39.0	35.0	39.0	33.0	39.0
4	36.92378958415524	38.0	35.0	39.0	33.0	39.0
5	37.62245001419962	39.0	36.0	40.0	33.0	41.0
6	37.63818396658211	39.0	36.0	40.0	33.0	41.0
7	37.68389474306369	39.0	36.0	40.0	33.0	41.0
8	37.594162248605386	39.0	36.0	40.0	33.0	41.0
9	37.47336549351983	39.0	36.0	40.0	33.0	41.0
10	37.46223452589353	39.0	36.0	40.0	33.0	41.0
11	37.34231041099142	39.0	35.0	40.0	33.0	41.0
12	37.52174128236781	39.0	36.0	40.0	33.0	41.0
13	37.79714959964748	39.0	36.0	40.0	33.0	41.0
14	37.80751892922821	39.0	36.0	41.0	33.0	41.0
15	37.8777683861106	39.0	36.0	41.0	33.0	41.0
16	37.93569420304549	39.0	36.0	41.0	33.0	41.0
17	37.919367758732285	39.0	36.0	41.0	33.0	41.0
18	37.93415795267849	40.0	36.0	41.0	33.0	41.0
19	37.886091143807356	39.0	36.0	41.0	33.0	41.0
20	37.8763964765711	39.0	36.0	41.0	33.0	41.0
21	37.84530818950708	39.0	36.0	41.0	33.0	41.0
22	37.780875532966434	40.0	36.0	41.0	33.0	41.0
23	37.749346120523334	39.0	35.0	41.0	33.0	41.0
24	37.66833666419835	39.0	35.0	41.0	33.0	41.0
25	37.53709914449931	39.0	35.0	41.0	33.0	41.0
26	37.49421785421785	39.0	35.0	41.0	33.0	41.0
27	37.479228859823166	39.0	35.0	41.0	33.0	41.0
28	37.49446056266147	39.0	35.0	41.0	33.0	41.0
29	37.425234577260845	39.0	35.0	41.0	32.0	41.0
30	37.436082599801885	39.0	35.0	41.0	33.0	41.0
31	37.29666599319147	39.0	35.0	41.0	32.0	41.0
32	37.264624740433106	39.0	35.0	41.0	32.0	41.0
33	37.19317462771165	39.0	35.0	40.0	32.0	41.0
34	37.205792922632845	39.0	35.0	40.0	32.0	41.0
35	37.157179709542675	39.0	35.0	40.0	32.0	41.0
36	37.100621002648325	39.0	35.0	40.0	32.0	41.0
37	37.07614767060809	39.0	35.0	40.0	32.0	41.0
38	37.02728493911804	38.0	35.0	40.0	32.0	41.0
39	36.90092137565635	38.0	35.0	40.0	31.0	41.0
40	36.78443772205205	38.0	35.0	40.0	31.0	41.0
41	36.731157803226466	38.0	35.0	40.0	31.0	41.0
42	36.56493663657959	38.0	35.0	40.0	31.0	41.0
43	36.45442559446761	38.0	34.0	40.0	31.0	41.0
44	36.43776915050939	38.0	34.0	40.0	31.0	41.0
45	36.33277306594687	37.0	34.0	40.0	31.0	41.0
46	36.233710815119665	37.0	34.0	40.0	31.0	41.0
47	36.069992236982266	37.0	34.0	40.0	30.0	41.0
48	35.983411064976956	37.0	34.0	40.0	30.0	41.0
49	36.031536527871516	37.0	34.0	40.0	30.0	41.0
50	35.894181527710394	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	372.0
15	2938.0
16	1446.0
17	24.0
18	0.0
19	4.0
20	12.0
21	31.0
22	79.0
23	213.0
24	462.0
25	1072.0
26	2203.0
27	4347.0
28	8752.0
29	16475.0
30	29618.0
31	50608.0
32	80486.0
33	123902.0
34	172692.0
35	149663.0
36	199791.0
37	251109.0
38	299641.0
39	405429.0
40	279664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	56.56933236779093	13.221027360825662	30.20964027138341
2	26.634650638790873	26.803172066287978	29.160165513317615	17.402011781603534
3	27.552941052098447	18.611646767170853	28.51654532515217	25.31886685557853
4	22.247134967547492	21.56617769664317	30.471480379290945	25.715206956518394
5	20.299407844439394	27.546886390032682	28.147066780519943	24.00663898500798
6	21.386075526623454	31.787936902732817	25.570808071537275	21.255179499106458
7	27.702001065812738	30.309109719606674	22.574375036339987	19.4145141782406
8	26.427831071084135	27.017535935140153	25.16038847865083	21.394244515124882
9	21.506159657594445	27.546117544056077	28.491125355050666	22.45659744329881
10	23.822164003496326	27.806708277251623	25.7682092760306	22.602918443221444
11	26.2858348297799	26.525186192871743	26.623166002015335	20.565812975333017
12	27.03959220409401	25.270429559052417	26.124473280439936	21.56550495641364
13	24.8281509110104	25.07105818674404	27.463754918812267	22.63703598343329
14	20.165052010027672	28.321306499968046	29.280009379920912	22.233632110083366
15	20.126561658323887	28.90298653414325	28.31630900112011	22.654142806412754
16	20.440010552411028	35.323138756036045	23.410639194518513	20.826211497034418
17	23.527119359974165	32.346743769104016	23.338944307200098	20.787192563721714
18	21.18795352902706	28.340047120647792	25.05380720514396	25.418192145181184
19	21.76617375630754	27.47836299236776	26.48193860668771	24.273524644636986
20	23.822500373611092	26.286891992997734	26.50298576529727	23.387621868093902
21	24.044552702229318	26.714995043346097	24.60499336630081	24.63545888812378
22	22.376108880122977	28.698281292872174	25.42448707161464	23.50112275539021
23	22.477836813402526	29.27952885118554	25.721357724331234	22.521276611080705
24	24.4804403180908	28.49684364700167	24.37712663998451	22.64558939492302
25	22.954030036977084	28.11498254801838	24.744081196519097	24.18690621848544
26	23.577199995110444	28.47008202232083	26.706320974977693	21.246397007591035
27	24.556060839488865	26.637629384325283	26.8886087650328	21.917701011153053
28	24.007240289849893	28.745946450450884	25.438766330864965	21.808046928834266
29	24.68973491006726	26.656568288324873	27.135949543447612	21.51774725816026
30	26.368626732489876	28.364161116524794	24.69376907651545	20.573443074469886
31	23.394903857185355	29.075345629839756	25.456550189300682	22.07320032367421
32	21.841978743645086	27.968320052373695	26.429126730019743	23.760574473961476
33	21.243468851445353	29.78641727211831	27.12603699695986	21.84407687947648
34	25.143559379999925	26.84084253332939	26.447257594180922	21.568340492489764
35	23.294208281629196	28.82644209406865	25.740575105288034	22.138774519014127
36	22.875629036266577	28.53330139233553	26.184477202728424	22.40659236866947
37	22.133379938347517	30.417314966849705	26.44724771007715	21.00205738472563
38	25.730056551397258	28.889466653613464	23.507855727868535	21.872621067120747
39	23.779355231196043	28.880305298719005	25.588345061763018	21.751994408321938
40	22.35765545683553	30.16349559568437	24.92157108113843	22.55727786634167
41	24.375292938628604	30.924275570317896	23.88101560107439	20.819415889979112
42	24.11315857830662	28.777200356459048	25.31474015763731	21.79490090759702
43	21.806380836132313	30.303855463588842	25.86716199343677	22.022601706842075
44	21.227905977226648	31.32214930201126	25.701141495055975	21.74880322570612
45	23.822279215816486	26.984087910074795	27.6295594477855	21.564073426323215
46	24.37700443060267	28.49240170179634	24.79776106260312	22.332832804997867
47	23.670157480037535	29.286248653803465	24.256376890400517	22.78721697575848
48	23.0599506687906	27.48499766169825	26.278881656521925	23.17617001298922
49	22.159100912585465	29.396532892527492	25.897082269628413	22.547283925258633
50	21.499711681866668	30.856329358953626	26.01111454152516	21.632844417654546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18381.0
1	14976.5
2	9897.5
3	7501.0
4	6678.0
5	6718.0
6	6787.5
7	6551.0
8	6619.5
9	6779.0
10	6512.0
11	6296.0
12	6218.0
13	6213.5
14	6636.5
15	8628.0
16	10075.5
17	10356.5
18	11536.5
19	15648.0
20	18660.0
21	18856.0
22	19932.0
23	24209.5
24	27566.0
25	31150.0
26	36299.0
27	43748.5
28	52165.0
29	60226.5
30	66515.5
31	67333.0
32	68152.5
33	77377.5
34	88347.0
35	87657.0
36	85976.5
37	94093.5
38	100410.5
39	99750.5
40	100922.0
41	107948.0
42	118071.0
43	129797.0
44	138721.0
45	133136.5
46	124895.0
47	119172.0
48	115838.0
49	123089.5
50	134736.0
51	136814.0
52	134056.5
53	129228.5
54	123114.5
55	124033.5
56	121267.0
57	113771.5
58	106052.0
59	94373.5
60	85179.0
61	76718.0
62	68302.0
63	63111.0
64	60249.5
65	56149.0
66	50118.0
67	46744.5
68	40155.5
69	31879.0
70	28244.5
71	25329.0
72	20644.5
73	17660.0
74	14299.0
75	11157.0
76	9334.0
77	7711.5
78	6212.5
79	4808.5
80	3769.5
81	3101.5
82	2591.5
83	1770.5
84	1180.0
85	922.5
86	550.5
87	283.5
88	258.5
89	338.5
90	330.5
91	289.0
92	156.0
93	27.0
94	10.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	16519.0
25	19347.0
26	13534.0
27	17357.0
28	14623.0
29	12925.0
30	16854.0
31	14702.0
32	14480.0
33	13797.0
34	16192.0
35	15550.0
36	12674.0
37	13214.0
38	15089.0
39	16214.0
40	15916.0
41	16517.0
42	17088.0
43	20154.0
44	21510.0
45	20609.0
46	23230.0
47	50037.0
48	119354.0
49	309213.0
50	1224342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.339089864872552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.59224900265836	7.19520516337072
2	13.182556333460907	2.725912691607943
3	5.246488809870607	1.6273175784090137
4	2.737252966547402	1.132028176160903
5	1.5103192627534248	0.780766329112786
6	1.004421658703475	0.6230883474935748
7	0.7140343601464746	0.5167725791312822
8	0.569131593860376	0.4707442155088461
9	0.43599153242402255	0.4056980070649918
>10	3.091022819005362	6.52430702970239
>50	0.4857504523890764	3.567502969051659
>100	0.7639192104696244	19.734434272217626
>500	0.5944173795751485	44.543997058130394
>1k	0.07151584098013505	9.511196645209404
>5k	9.287771555861696E-4	0.6410289378284589
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	7148	0.34348194004827387	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	6203	0.2980719745550424	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	4225	0.20302339069725198	No Hit
ATCAAGGGAAAGGCAAGACTGGGACGTCTAGTCCACAGGAAGACCTGCAC	3648	0.175296882665935	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	3367	0.16179402520181005	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	2571	0.1235439378657124	No Hit
CAGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGAT	2452	0.11782564591471288	No Hit
AACAAGAGCTGGAAGGAATGTCTCCAGATGCTAATAAGCTTGCAGGACTT	2344	0.11263593557262928	No Hit
TACCACACTACCCTGTTGACACAGCCTAGATCCAGAGTTCAGAAGACCT	2337	0.1122995654578646	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2324	0.11167487810187304	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2298	0.11042550338988998	No Hit
ACATCAGAAATCTTTAGTTCCTGATGAATTAAAAAAAGAGGTACTAGTTC	2144	0.10302536086506706	No Hit
AGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCG	2116	0.10167988040600834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.051080204570693226	0.0	0.0	0.0	0.0
2	0.08909002753910182	0.0	0.0	0.0	0.0
3	0.11532689649074669	0.0	0.0	0.0	0.0
4	0.14699374015216424	0.0	0.0	0.0	0.0
5	0.18524382748826188	0.0	0.0	0.0	0.0
6	0.2414176366539631	0.0	0.0	0.0	0.0
7	0.2939874803043285	0.0	0.0	0.0	0.0
8	0.3563601101564073	0.0	0.0	0.0	0.0
9	0.4120533905867304	0.0	0.0	0.0	0.0
10	0.4672180894081376	0.0	0.0	0.0	0.0
11	0.5265633882273343	0.0	0.0	0.0	0.0
12	0.5896568111824803	0.0	0.0	0.0	0.0
13	0.6451578801186522	0.0	0.0	0.0	0.0
14	0.7154111812309321	0.0	0.0	0.0	0.0
15	0.7817721995866492	0.0	0.0	0.0	0.0
16	0.8494786984014251	0.0	0.0	0.0	0.0
17	0.9211735857198392	0.0	0.0	0.0	0.0
18	0.9801825144242713	0.0	0.0	0.0	0.0
19	1.0538475695577358	0.0	0.0	0.0	0.0
20	1.1299633212416287	0.0	0.0	0.0	0.0
21	1.1950749648853627	0.0	0.0	0.0	0.0
22	1.2541799993368703	0.0	0.0	0.0	0.0
23	1.3099213326407313	0.0	0.0	0.0	0.0
24	1.3635002866353907	0.0	0.0	0.0	0.0
25	1.4192896728127893	0.0	0.0	0.0	0.0
26	1.4760401164609442	0.0	0.0	0.0	0.0
27	1.5250540474695116	0.0	0.0	0.0	0.0
28	1.5739238198574657	0.0	0.0	0.0	0.0
29	1.6355276037329394	0.0	0.0	0.0	0.0
30	1.6895390335894391	0.0	0.0	0.0	0.0
31	1.7469622174671233	0.0	0.0	0.0	0.0
32	1.8018385990473038	4.805287353781112E-5	0.0	0.0	0.0
33	1.858973465683761	4.805287353781112E-5	0.0	0.0	0.0
34	1.9191356633531007	4.805287353781112E-5	0.0	0.0	0.0
35	1.9836226196408433	4.805287353781112E-5	0.0	0.0	0.0
36	2.0438809230572583	4.805287353781112E-5	0.0	0.0	0.0
37	2.1004872080848	4.805287353781112E-5	0.0	0.0	0.0
38	2.1581026034566353	4.805287353781112E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAC	15	0.0022621946	69.707504	44
ACAAGCG	15	0.0022621946	69.707504	44
TCGCATC	25	2.9126422E-6	69.7075	44
ACCGACT	115	0.0	69.7075	44
ATAGACC	55	0.0	69.7075	44
CGATTAT	55	0.0	69.7075	44
CCGCGTA	75	0.0	69.7075	44
CAATCGT	75	0.0	69.7075	44
TATCGTC	25	2.9126422E-6	69.7075	44
TACGGAT	75	0.0	65.06033	44
TTGCGTG	65	0.0	64.34538	44
CGGCACA	160	0.0	63.17242	44
ATCGGGA	90	0.0	61.96222	44
TCTACGG	130	0.0	61.664326	44
GCCCGAC	85	0.0	61.506615	44
TTCCGTT	110	0.0	60.201927	44
GACGTGT	30	8.590992E-6	58.089584	44
ATAGAAC	120	0.0	58.089584	44
TACTGCG	80	0.0	56.637344	44
AACCTAT	100	0.0	55.766	44
>>END_MODULE