FastQCFastQC Report
Tue 24 May 2016
ERR840909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12401764
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA706060.5693222351271964No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT284200.22916094839411555No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC277340.2236294772259817No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA177140.14283451934740898No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA161170.1299573189749458No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGG130710.10539629684938369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG750.055.6331944
TAAGCCA108550.050.67463344
CGGTTAT303.8342732E-641.41816718
TTAAGCC106100.040.9925743
CGCTTAT2000.039.60272219
TACGGTA900.039.34609637
GACGCTA37200.039.33352731
CGCTACT40950.038.36609333
CGACTAG1800.037.96665612
TTGCGCG105450.037.85872722
GTTGCGC117750.037.77100821
GCGCAAC1100.037.6528812
TCGACGA1100.037.652883
TAGCGCA405.016336E-737.55529824
GCCCTCG96900.037.3308130
TCGGACC66800.037.2147634
GCCGGTT122700.037.02993417
ATTAAGC109450.036.87317342
TCGTAGG454.0106897E-836.8161518
TAATCCG1450.036.5733722
Produced by FastQC (version 0.11.5)