##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840909.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12401764
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.21434878135078	39.0	37.0	39.0	34.0	39.0
2	37.625929988669355	39.0	37.0	39.0	35.0	39.0
3	37.62711070779931	39.0	37.0	39.0	35.0	39.0
4	37.783411295360885	39.0	37.0	39.0	35.0	40.0
5	38.58656792694975	40.0	38.0	41.0	35.0	41.0
6	38.579187283357435	40.0	38.0	41.0	35.0	41.0
7	38.59925878286347	40.0	38.0	41.0	35.0	41.0
8	38.493656386301176	40.0	38.0	41.0	34.0	41.0
9	38.3769622611751	40.0	38.0	41.0	34.0	41.0
10	38.38053763964545	40.0	38.0	41.0	34.0	41.0
11	38.29180316606573	40.0	38.0	41.0	34.0	41.0
12	38.458060321096255	40.0	38.0	41.0	34.0	41.0
13	38.63657250694337	40.0	38.0	41.0	35.0	41.0
14	38.62527411423085	40.0	38.0	41.0	34.0	41.0
15	38.66962901406606	40.0	38.0	41.0	34.0	41.0
16	38.67702489742588	40.0	38.0	41.0	35.0	41.0
17	38.68909729293349	40.0	38.0	41.0	35.0	41.0
18	38.668689550938076	40.0	38.0	41.0	35.0	41.0
19	38.66732297115152	40.0	38.0	41.0	35.0	41.0
20	38.64005152815358	40.0	38.0	41.0	34.0	41.0
21	38.58034429618238	40.0	38.0	41.0	34.0	41.0
22	38.424078945543556	40.0	38.0	41.0	34.0	41.0
23	38.448506357643964	40.0	38.0	41.0	34.0	41.0
24	38.31647618838739	40.0	38.0	41.0	34.0	41.0
25	38.22957340497008	40.0	37.0	41.0	33.0	41.0
26	38.174785092098254	40.0	37.0	41.0	33.0	41.0
27	38.14111165588134	40.0	37.0	41.0	33.0	41.0
28	38.121964225448295	40.0	37.0	41.0	33.0	41.0
29	38.092587138233476	40.0	37.0	41.0	33.0	41.0
30	38.06652487179185	40.0	37.0	41.0	33.0	41.0
31	37.91857608302079	40.0	37.0	41.0	33.0	41.0
32	37.82716037458168	40.0	36.0	41.0	33.0	41.0
33	37.85060688992951	40.0	36.0	41.0	33.0	41.0
34	37.78606741850716	40.0	36.0	41.0	33.0	41.0
35	37.745224169434394	40.0	36.0	41.0	33.0	41.0
36	37.707003836746175	39.0	36.0	41.0	33.0	41.0
37	37.65404917480421	39.0	36.0	41.0	33.0	41.0
38	37.557341481917874	39.0	36.0	41.0	32.0	41.0
39	37.47587640622852	39.0	35.0	41.0	32.0	41.0
40	37.442755501190064	39.0	35.0	41.0	32.0	41.0
41	37.400524141057524	39.0	35.0	41.0	32.0	41.0
42	37.26219367809693	39.0	35.0	41.0	32.0	41.0
43	37.206329940984745	39.0	35.0	41.0	32.0	41.0
44	37.14616862387487	39.0	35.0	40.0	31.0	41.0
45	37.043086752651455	39.0	35.0	40.0	31.0	41.0
46	36.90235816663124	38.0	35.0	40.0	31.0	41.0
47	36.82537511418748	38.0	35.0	40.0	31.0	41.0
48	36.76383972438795	38.0	35.0	40.0	31.0	41.0
49	36.91288690950456	38.0	35.0	40.0	31.0	41.0
50	36.81846693680266	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	14.0
14	568.0
15	9580.0
16	18067.0
17	753.0
18	0.0
19	4.0
20	20.0
21	63.0
22	119.0
23	330.0
24	771.0
25	1663.0
26	3844.0
27	8892.0
28	20502.0
29	44644.0
30	90334.0
31	168129.0
32	287964.0
33	457673.0
34	679548.0
35	816790.0
36	1098930.0
37	1427066.0
38	1800889.0
39	2657458.0
40	2807149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	59.93473186556364	11.946018324489968	28.11924980994639
2	26.132959795074314	24.452327910771405	31.51121082452464	17.903501469629642
3	26.368200523731943	18.71474090298767	28.73045318391803	26.18660538936235
4	22.33035558489905	20.634629073734995	32.584364611356904	24.450650730009055
5	19.963216523068816	24.718112681389517	30.54854132041216	24.770129475129508
6	20.711223016338643	28.771350591738404	28.319543897142374	22.197882494780583
7	27.861988020413868	26.663634302346022	24.61452257920728	20.859855098032828
8	25.501388350882987	25.727622296312042	26.275254068695386	22.495735284109582
9	24.11493235962239	25.148607891587037	29.64052533171894	21.095934417071636
10	25.175096058915493	25.310205870713233	25.940366225320847	23.57433184505043
11	24.539162332068244	25.718663893297762	27.3350468530122	22.407126921621796
12	25.09646208394225	23.333898306724755	28.873432843908336	22.696206765424662
13	24.277852731272745	21.955207339859072	28.871755663145986	24.895184265722197
14	20.225284080554992	24.09826537579654	29.319401659312337	26.35704888433613
15	20.45231629951997	26.55010206612543	29.659861290700256	23.33772034365434
16	24.290213876026023	29.610537662222892	24.164376938635503	21.934871523115582
17	24.894539196198217	29.65591830323493	22.69519884429344	22.754343656273416
18	22.1422694384444	25.593383328371672	25.40571647710761	26.858630756076312
19	23.204102255130802	24.96735141871753	26.16860794964329	25.65993837650837
20	26.236735354744695	23.15732665127316	25.8339378172331	24.77200017674905
21	24.989541810342462	24.885846884362582	26.14740128904243	23.977210016252528
22	24.037225672089875	26.13308074561006	26.52557329747607	23.304120284824
23	25.64240054882515	25.21009914396049	26.785641139437903	22.361859167776455
24	25.510330627159167	26.03955372800192	25.996334069895216	22.453781574943694
25	24.41952463979661	27.3432354940207	25.83956824453979	22.3976716216429
26	24.741156859498357	26.142879584622975	27.053182459347862	22.062781096530806
27	24.25102190947977	26.049628576826876	26.770189614800007	22.929159898893346
28	25.05585592252839	24.72015047110599	26.485090247477462	23.73890335888816
29	25.3425646014986	25.45169640338637	26.0086964794089	23.19704251570613
30	25.33652680923184	25.562553119817256	26.052494013277023	23.048426057673886
31	24.02249200533799	26.627620453847218	26.967981045227113	22.38190649558767
32	23.484432166929913	26.01671190243917	27.22990159969756	23.268954330933354
33	24.040264510987818	25.56199889724574	26.506304692297807	23.89143189946864
34	23.400295208791015	26.944130779502405	27.731554449082747	21.92401956262383
35	22.962906954789357	25.032791425449464	27.328999164822115	24.675302454939064
36	24.332989474006602	25.809165730450484	27.711085426072334	22.14675936947058
37	23.712654537817045	26.750583388388428	27.285549861784926	22.251212212009598
38	23.78906355872985	27.11384884748834	26.59973018349059	22.49735741029122
39	23.93926791830809	26.456920136583133	25.974502524350612	23.62930942075816
40	22.953287341750823	27.409341814637166	26.729416627738857	22.907954215873154
41	23.854820874071137	26.462910292318814	26.963409011993207	22.718859821616846
42	22.946647853180522	27.996883557369372	27.020528081895655	22.035940507554454
43	22.992410382335393	27.26481700274209	26.853852009251995	22.88892060567052
44	24.2881751960621	27.148686980172066	26.290064611868296	22.273073211897536
45	23.833852126428045	26.75500882844748	26.905730890211533	22.505408154912942
46	23.08021898917173	28.048534167224602	26.112469173960047	22.75877766964362
47	24.041258799490866	27.618789712878566	26.310184214956962	22.029767272673613
48	22.844723278277723	26.973348265334806	26.476704179895155	23.705224276492316
49	23.01695874495554	27.38583967644233	26.125010589077117	23.472190989525004
50	21.805708523334626	29.894494565035295	27.97478796774364	20.325008943886434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23819.0
1	21514.0
2	19038.5
3	18646.5
4	17785.5
5	14710.0
6	12121.0
7	12300.0
8	13253.5
9	15077.5
10	16987.0
11	20336.5
12	23928.5
13	27947.0
14	33021.0
15	40539.0
16	46121.0
17	56077.0
18	67041.5
19	78787.5
20	88808.0
21	108054.5
22	129519.5
23	155985.0
24	182390.0
25	206094.5
26	229743.5
27	251051.5
28	275817.0
29	315658.5
30	350382.5
31	383237.5
32	428576.0
33	467445.5
34	498148.0
35	522092.0
36	553205.0
37	578191.5
38	597548.5
39	618662.0
40	650383.0
41	683386.0
42	698493.0
43	736554.5
44	782948.5
45	753001.5
46	726560.0
47	714950.5
48	704143.0
49	728832.5
50	768293.5
51	763813.0
52	731813.0
53	705111.0
54	680664.5
55	646190.0
56	603572.5
57	586282.5
58	577612.5
59	568473.5
60	562040.0
61	538183.5
62	477736.0
63	413166.0
64	375964.5
65	341667.5
66	301694.0
67	277881.0
68	261765.0
69	250167.0
70	229880.5
71	201796.0
72	171537.0
73	158754.5
74	159617.0
75	144755.5
76	117939.0
77	87040.0
78	61434.5
79	49957.5
80	41636.0
81	30495.0
82	20678.5
83	16136.5
84	13036.5
85	9774.0
86	6711.5
87	4562.5
88	3060.5
89	2192.0
90	1542.0
91	799.5
92	487.5
93	460.0
94	271.0
95	73.5
96	8.5
97	8.5
98	6.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	81086.0
25	73106.0
26	69145.0
27	71385.0
28	71137.0
29	66502.0
30	85536.0
31	81261.0
32	71978.0
33	77181.0
34	81062.0
35	79313.0
36	75721.0
37	90394.0
38	85452.0
39	84101.0
40	84049.0
41	89036.0
42	96287.0
43	95278.0
44	102879.0
45	119672.0
46	134514.0
47	266032.0
48	737768.0
49	2030185.0
50	7401704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.424806940610125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.66737250860439	6.471767290125556
2	13.707238316489507	2.583761496436947
3	5.598292425615979	1.5828847592553172
4	2.802028729242906	1.0563431926063005
5	1.661335225650417	0.7828881882695069
6	1.0851135178887845	0.6136191248848852
7	0.7678414410241494	0.5065730139856739
8	0.5733296871674872	0.4322817291899172
9	0.4208576648677161	0.3569852016768799
>10	2.8653627927422365	5.389173089908702
>50	0.43020959435583717	2.8664137233207656
>100	0.6610829331747514	15.817617312501584
>500	0.6500927828389014	45.181039000606845
>1k	0.1071112566611052	13.518024287737788
>5k	0.0021336903717351636	1.345607057098203
>10k+	5.974333040858458E-4	1.4950215323952971
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	70606	0.5693222351271964	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	28420	0.22916094839411555	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	27734	0.2236294772259817	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA	17714	0.14283451934740898	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	16117	0.1299573189749458	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGG	13071	0.10539629684938369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06132998499245752	0.0	0.0	0.0	0.0
2	0.11385477098257958	0.0	0.0	0.0	0.0
3	0.14316511747844904	0.0	0.0	0.0	0.0
4	0.18095812821466364	0.0	0.0	0.0	0.0
5	0.2234037028925885	0.0	0.0	0.0	0.0
6	0.2968771216739812	0.0	0.0	0.0	0.0
7	0.35639284863024323	0.0	0.0	0.0	0.0
8	0.42345588901707854	0.0	0.0	0.0	0.0
9	0.48572122481930796	0.0	0.0	0.0	0.0
10	0.5528810256347404	0.0	0.0	0.0	0.0
11	0.6223550133674532	0.0	0.0	0.0	0.0
12	0.690732382909399	0.0	0.0	0.0	0.0
13	0.7656894615959472	0.0	0.0	0.0	0.0
14	0.8427268894973328	0.0	0.0	0.0	0.0
15	0.9262956463290222	0.0	0.0	0.0	0.0
16	1.0051795857428023	0.0	0.0	0.0	0.0
17	1.0960215014573733	0.0	0.0	0.0	0.0
18	1.169761011417408	0.0	0.0	0.0	0.0
19	1.2553214204043877	0.0	0.0	0.0	0.0
20	1.3414301384867509	0.0	0.0	0.0	0.0
21	1.4161372527327565	0.0	0.0	0.0	0.0
22	1.4888607781925216	0.0	0.0	0.0	0.0
23	1.554996531138635	0.0	0.0	0.0	0.0
24	1.6217370367634798	0.0	0.0	0.0	0.0
25	1.6857359969114072	0.0	0.0	0.0	0.0
26	1.7563307929420362	0.0	0.0	0.0	0.0
27	1.819257325006346	0.0	0.0	0.0	0.0
28	1.8824176947730984	0.0	0.0	0.0	0.0
29	1.9557782263877945	0.0	0.0	0.0	0.0
30	2.020962501786036	0.0	0.0	0.0	0.0
31	2.089436631756579	0.0	0.0	0.0	0.0
32	2.1544031961904775	0.0	0.0	0.0	0.0
33	2.2197003587554156	0.0	0.0	0.0	0.0
34	2.289190473226228	0.0	0.0	0.0	0.0
35	2.3580919617564082	0.0	0.0	0.0	0.0
36	2.428501300298893	0.0	0.0	0.0	0.0
37	2.4943951521735133	0.0	0.0	0.0	0.0
38	2.5601599901433376	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	75	0.0	55.63319	44
TAAGCCA	10855	0.0	50.674633	44
CGGTTAT	30	3.8342732E-6	41.418167	18
TTAAGCC	10610	0.0	40.99257	43
CGCTTAT	200	0.0	39.602722	19
TACGGTA	90	0.0	39.346096	37
GACGCTA	3720	0.0	39.333527	31
CGCTACT	4095	0.0	38.366093	33
CGACTAG	180	0.0	37.966656	12
TTGCGCG	10545	0.0	37.858727	22
GTTGCGC	11775	0.0	37.771008	21
GCGCAAC	110	0.0	37.65288	12
TCGACGA	110	0.0	37.65288	3
TAGCGCA	40	5.016336E-7	37.555298	24
GCCCTCG	9690	0.0	37.33081	30
TCGGACC	6680	0.0	37.21476	34
GCCGGTT	12270	0.0	37.029934	17
ATTAAGC	10945	0.0	36.873173	42
TCGTAGG	45	4.0106897E-8	36.81615	18
TAATCCG	145	0.0	36.57337	22
>>END_MODULE