FastQCFastQC Report
Tue 24 May 2016
ERR840908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3859012
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA314340.8145608254133442No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC198150.513473396817631No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT192950.49999844519789005No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT186950.48445042409818884No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA127700.3309137157386398No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA110980.2875865636074726No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT109920.2848397465465254No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC93770.24298965641982975No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG75480.1955941054342407No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG75370.19530905838074616No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC71400.18502145108644388No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG69750.18074574528402607No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCTG64600.16740036050678256No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT63480.16449806323483834No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG63310.1640575359703468No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG58740.15221512656607442No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT57810.14980518329562076No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT55000.1425235267472607No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG54950.1423939599047632No Hit
TGATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGACTTGT52390.1357601375688907No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC51600.13371298145743005No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA49590.12850439438903014No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG48880.1266645452255655No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG48120.12469512921960337No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA47920.12417686184961332No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG44970.11653241814226026No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG43830.11357829413331702No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG43520.11277497970983245No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT42850.11103878402036584No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGACT42840.11101287065186634No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA42640.1104946032818763No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC41330.10709995200844154No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGG40170.1040940012624993No Hit
ACCGCGGCCTCAGATGAATGCGGCTG40150.1040421745255003No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT39590.1025910258895282No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT38690.10025882272457302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAT151.4398679E-4139.3781144
TCGCTAG600.0139.3781144
GCGATAC450.0139.3781144
ATACGAG1200.0139.3781144
AAATCGA850.0131.179444
CGATTCA650.0128.6567144
TCGTAGG1600.0126.3114244
CGAACCG1500.0125.4403144
TAAGCCA39850.0122.0651444
ACACGGC700.0119.4669444
GTAACGA406.339178E-9104.53358544
CTATCAT28750.0103.9881844
TAACACC1400.084.6224244
ATACTGC1500.083.6268744
GGATATC1450.081.7044144
GAACACA23500.080.9579344
ACATTGG8500.073.7884144
CGACGGG11650.072.38090544
CTATATA1650.071.8008444
TTGTACA1650.071.8008444
Produced by FastQC (version 0.11.5)