##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840908.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3859012
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.865692306735504	38.0	35.0	39.0	30.0	39.0
2	36.78304343184214	38.0	37.0	39.0	33.0	39.0
3	36.927228005510216	38.0	35.0	39.0	33.0	39.0
4	37.27167575534878	39.0	36.0	40.0	33.0	41.0
5	37.682045041580594	39.0	36.0	40.0	33.0	41.0
6	37.64858673670877	39.0	36.0	40.0	33.0	41.0
7	37.550316505882854	39.0	36.0	40.0	33.0	41.0
8	37.42749828194367	39.0	36.0	40.0	33.0	41.0
9	37.22548491686473	38.0	36.0	40.0	32.0	41.0
10	37.141033248924856	38.0	35.0	40.0	32.0	41.0
11	37.172914466189795	38.0	35.0	40.0	32.0	41.0
12	37.4809065118222	39.0	36.0	40.0	33.0	41.0
13	37.55261320773296	39.0	36.0	40.0	33.0	41.0
14	37.601353921677365	39.0	36.0	40.0	33.0	41.0
15	37.60521397704905	39.0	36.0	40.0	33.0	41.0
16	37.59774574424749	39.0	36.0	40.0	33.0	41.0
17	37.59233503290479	39.0	36.0	40.0	33.0	41.0
18	37.64163107033614	39.0	36.0	40.0	33.0	41.0
19	37.59018422331933	39.0	36.0	40.0	33.0	41.0
20	37.526883305882436	39.0	36.0	40.0	33.0	41.0
21	37.39479457436256	39.0	35.0	40.0	32.0	41.0
22	37.28832509460971	39.0	35.0	40.0	32.0	41.0
23	37.09099453435232	39.0	35.0	40.0	31.0	41.0
24	36.88946834060117	38.0	35.0	40.0	31.0	41.0
25	36.79904997297075	38.0	35.0	40.0	31.0	41.0
26	36.658543550866185	38.0	35.0	40.0	31.0	41.0
27	36.58302846163584	38.0	35.0	40.0	31.0	41.0
28	36.581397966548955	38.0	35.0	40.0	31.0	41.0
29	36.45190231897631	38.0	34.0	40.0	30.0	41.0
30	36.40782431443797	38.0	34.0	40.0	30.0	41.0
31	36.259251522450214	38.0	34.0	40.0	30.0	41.0
32	36.116220258812426	37.0	34.0	40.0	30.0	41.0
33	36.022155747975646	37.0	34.0	40.0	30.0	41.0
34	35.996043254319076	37.0	34.0	40.0	30.0	41.0
35	35.84755271574488	37.0	34.0	40.0	30.0	41.0
36	35.75700269602537	37.0	34.0	40.0	29.0	41.0
37	35.6915563037572	36.0	34.0	40.0	30.0	41.0
38	35.567515879482286	36.0	33.0	40.0	29.0	41.0
39	35.47776934728653	36.0	33.0	40.0	29.0	41.0
40	35.379113124883816	36.0	33.0	39.0	29.0	41.0
41	35.30443916924024	36.0	33.0	39.0	29.0	41.0
42	35.165803746397785	35.0	33.0	39.0	29.0	41.0
43	35.08499570094012	35.0	33.0	39.0	29.0	41.0
44	34.93584013368557	35.0	33.0	39.0	29.0	40.0
45	34.751321416415365	35.0	33.0	39.0	29.0	40.0
46	34.608697410730535	35.0	33.0	38.0	29.0	40.0
47	34.4583561363587	35.0	33.0	38.0	28.0	40.0
48	34.388345423307854	35.0	33.0	38.0	28.0	40.0
49	34.52718549657735	35.0	33.0	38.0	28.0	40.0
50	35.075114639934114	36.0	33.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	18.0
14	836.0
15	3441.0
16	744.0
17	8.0
18	0.0
19	2.0
20	22.0
21	72.0
22	187.0
23	548.0
24	1327.0
25	2766.0
26	5578.0
27	11370.0
28	21980.0
29	42147.0
30	74659.0
31	124376.0
32	192301.0
33	274817.0
34	372351.0
35	406710.0
36	482249.0
37	529309.0
38	529428.0
39	535931.0
40	245835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.6628857334468	18.534925519796257	37.80218874675694
2	26.296653132978076	20.125073464399694	30.862536835853323	22.715736566768904
3	23.38759247185549	17.652704889230712	28.388483891731873	30.571218747181923
4	19.70711674387123	19.34601395383067	33.69510123316538	27.25176806913272
5	19.681669816004717	21.538647716047528	33.40909538503637	25.370587082911378
6	24.31529106413766	23.906067148793525	27.218987657980854	24.559654129087964
7	27.626708597952014	22.279718228396284	22.989200344544148	27.104372829107554
8	25.518215543253042	21.272465594820645	26.698077124403863	26.511241737522457
9	25.381678004629165	21.038752924323635	27.43971773085961	26.13985134018759
10	27.410539277929168	18.677682266860014	25.47913818355579	28.432640271655025
11	27.069389781633223	21.144764514855098	25.875016714122683	25.910828989388996
12	28.566612386797452	19.50367088778164	24.84242080615453	27.08729591926638
13	25.54272958985357	19.47187518463275	26.70955674664914	28.27583847886454
14	24.490931875827286	22.557820499132937	23.852374649262558	29.09887297577722
15	24.098240689585833	23.56193761512014	26.074005470830357	26.265816224463673
16	26.580585911627118	24.56048335687995	22.771346655568834	26.0875840759241
17	26.356901714739422	22.99539363961553	22.099749884167245	28.547954761477808
18	25.52487527895741	21.39223718402534	20.870808383078362	32.21207915393888
19	25.140320890424807	23.101146096461996	23.73941827597323	28.019114737139972
20	30.554737844816238	20.728077549383105	23.59927877912792	25.117905826672732
21	27.65052298360306	25.06501664156525	21.163810840702233	26.12064953412946
22	29.819782887433366	24.02449124283625	22.106565100082612	24.049160769647777
23	30.91628116212129	22.086093538968004	22.374224283313968	24.623401015596738
24	30.350903288199156	22.74400805180186	22.842608418942465	24.06248024105652
25	30.211346213524255	23.177880708129656	22.766476197254374	23.84429688109172
26	27.911875866201207	24.484955376272787	23.376508438378256	24.226660319147754
27	29.0141173089671	22.735950395785693	23.57062847637865	24.679303818868554
28	29.369536240787898	21.56650663303675	24.21961336033372	24.84434376584163
29	31.117699481083903	22.86410867677762	23.07963616315723	22.93855567898125
30	29.211230685241546	22.66515486065525	23.126985074231	24.996629379872203
31	27.21081415746569	22.57985855062348	24.501709881405905	25.70761741050493
32	25.5418524341339	22.144274400479006	26.66338991530249	25.650483250084605
33	28.01332782543081	20.342322635130298	26.407652012093703	25.236697527345186
34	28.634804014544745	21.817641630153737	24.64577077921307	24.901783576088445
35	25.457315464106145	21.375341733136768	25.291360118408846	27.875982684348244
36	25.000934266215452	22.279579906454742	27.827519627005337	24.89196620032447
37	24.75696861942697	23.38602775418816	24.951441719714555	26.90556190667031
38	25.8037792621429	23.237046580219463	25.982457588941493	24.97671656869614
39	26.523161350288344	24.300203549792933	24.7849585024336	24.39167659748512
40	24.709834570423364	24.062266277644905	23.058958183476438	28.168940968455292
41	25.068701166669864	23.599082663721667	22.870263833234226	28.461952336374246
42	24.941548982953893	23.908938196296848	24.579507214920724	26.570005605828534
43	25.39571331522615	21.875543827851978	25.88907394254084	26.839668914381033
44	25.98901821883829	26.01742444352369	23.852935424162073	24.140621913475947
45	26.3030404166021	22.816070489669045	26.202220747727665	24.678668346001185
46	25.538279527850992	24.992146579211692	23.91717168993481	25.5524022030025
47	25.132900451835706	22.9015160725596	25.073661377742436	26.891922097862263
48	23.62747581103128	23.125115902844374	24.031304771986186	29.216103514138165
49	25.43186950287972	20.92638595573717	24.63813464108195	29.003609900301157
50	23.65900044669028	30.716344424480802	23.107750212245836	22.516904916583083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11138.0
1	8419.0
2	5334.5
3	4455.0
4	3865.0
5	3382.0
6	2554.0
7	2304.0
8	2394.0
9	2535.5
10	2784.5
11	3727.0
12	4526.5
13	4178.5
14	4188.0
15	4223.0
16	4375.5
17	5036.5
18	4978.5
19	4666.0
20	4823.5
21	8505.5
22	12147.5
23	12876.5
24	13468.5
25	16618.0
26	21504.5
27	27615.0
28	32401.0
29	34378.0
30	36083.0
31	43651.5
32	59849.5
33	69172.0
34	75099.5
35	86982.5
36	85924.5
37	79461.5
38	89606.0
39	114610.5
40	132759.0
41	138839.5
42	153189.5
43	182732.5
44	204776.0
45	196804.5
46	198366.0
47	214925.5
48	229425.0
49	254458.5
50	292697.0
51	307825.0
52	307520.0
53	325574.0
54	323740.0
55	307204.5
56	303079.0
57	292150.0
58	274070.5
59	256447.5
60	250902.5
61	256809.0
62	243502.0
63	230642.5
64	215087.0
65	187577.0
66	166598.0
67	151614.0
68	139561.0
69	129177.0
70	112485.0
71	93028.5
72	84379.5
73	79796.0
74	74538.0
75	67757.5
76	59113.5
77	48390.0
78	37912.0
79	30034.5
80	25181.5
81	21144.5
82	16670.0
83	13365.0
84	11105.5
85	8975.5
86	6871.0
87	4568.0
88	2237.5
89	1042.5
90	819.5
91	614.5
92	427.5
93	328.5
94	192.5
95	117.5
96	115.5
97	114.5
98	58.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	37226.0
25	36580.0
26	41008.0
27	39560.0
28	44316.0
29	40810.0
30	43533.0
31	41991.0
32	43246.0
33	37399.0
34	42787.0
35	38926.0
36	38007.0
37	39435.0
38	37491.0
39	39739.0
40	36881.0
41	35136.0
42	41937.0
43	40295.0
44	42465.0
45	53384.0
46	63371.0
47	131771.0
48	515423.0
49	1152622.0
50	1103673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.716921195948938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.70161251626716	5.8143269994702225
2	12.874018079396842	2.2444360214664836
3	5.419122707987506	1.417141967901135
4	2.976399038976734	1.0378014348183338
5	1.8543404789171523	0.8082069912589516
6	1.2695313059526647	0.6639842609867712
7	0.916275128347956	0.5590968661331991
8	0.7120029621784332	0.49651789700732923
9	0.5706575609546336	0.4476939289842573
>10	4.427930162929881	7.895659140080339
>50	0.677947048823498	4.185127659916171
>100	0.9542801104135069	20.55292992971941
>500	0.5295289942811661	33.09260351230845
>1k	0.11012065968515282	15.22515341666606
>5k	0.004155496591892559	2.3484235487332565
>10k+	0.0020777482959462796	3.2108964245496434
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	31434	0.8145608254133442	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	19815	0.513473396817631	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	19295	0.49999844519789005	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	18695	0.48445042409818884	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	12770	0.3309137157386398	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	11098	0.2875865636074726	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT	10992	0.2848397465465254	No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC	9377	0.24298965641982975	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	7548	0.1955941054342407	No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG	7537	0.19530905838074616	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	7140	0.18502145108644388	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	6975	0.18074574528402607	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCTG	6460	0.16740036050678256	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT	6348	0.16449806323483834	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG	6331	0.1640575359703468	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	5874	0.15221512656607442	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	5781	0.14980518329562076	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT	5500	0.1425235267472607	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	5495	0.1423939599047632	No Hit
TGATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGACTTGT	5239	0.1357601375688907	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	5160	0.13371298145743005	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	4959	0.12850439438903014	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	4888	0.1266645452255655	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	4812	0.12469512921960337	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	4792	0.12417686184961332	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	4497	0.11653241814226026	No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG	4383	0.11357829413331702	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	4352	0.11277497970983245	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	4285	0.11103878402036584	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGACT	4284	0.11101287065186634	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	4264	0.1104946032818763	No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC	4133	0.10709995200844154	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGG	4017	0.1040940012624993	No Hit
ACCGCGGCCTCAGATGAATGCGGCTG	4015	0.1040421745255003	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	3959	0.1025910258895282	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	3869	0.10025882272457302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.026638942817488	0.0	0.0	0.0	0.0
2	0.05136029636601285	0.0	0.0	0.0	0.0
3	0.06688240409721452	0.0	0.0	0.0	0.0
4	0.08512541552086389	0.0	0.0	0.0	0.0
5	0.1091989348569012	0.0	0.0	0.0	0.0
6	0.14780985392115908	0.0	0.0	0.0	0.0
7	0.1877164414103921	0.0	0.0	0.0	0.0
8	0.23759967577193333	0.0	0.0	0.0	0.0
9	0.2785687113696459	0.0	0.0	0.0	0.0
10	0.3214553362363216	0.0	0.0	0.0	0.0
11	0.37284154597083397	0.0	0.0	0.0	0.0
12	0.4158577376800072	0.0	0.0	0.0	0.0
13	0.4595476769701675	0.0	0.0	0.0	0.0
14	0.5105710995456868	0.0	0.0	0.0	0.0
15	0.5681765177200796	0.0	0.0	0.0	0.0
16	0.6178524451336249	0.0	0.0	0.0	0.0
17	0.6912131913557149	0.0	0.0	0.0	0.0
18	0.740578158347266	0.0	0.0	0.0	0.0
19	0.7997642920001286	0.0	0.0	0.0	0.0
20	0.8541564524805831	0.0	0.0	0.0	0.0
21	0.9023553178896567	0.0	0.0	0.0	0.0
22	0.9479110197117812	0.0	0.0	0.0	0.0
23	0.9896315429959793	0.0	0.0	0.0	0.0
24	1.0394111238835224	0.0	0.0	0.0	0.0
25	1.0847854321261505	0.0	0.0	0.0	0.0
26	1.138607498499616	0.0	0.0	0.0	0.0
27	1.1823751778952747	0.0	0.0	0.0	0.0
28	1.2280863599283962	0.0	0.0	0.0	0.0
29	1.2836446219913284	0.0	0.0	0.0	0.0
30	1.3320248809798985	0.0	0.0	0.0	0.0
31	1.3764403945880448	0.0	0.0	0.0	0.0
32	1.4230585445186488	0.0	0.0	0.0	0.0
33	1.4708168826632309	0.0	0.0	0.0	0.0
34	1.5188084411243086	0.0	0.0	0.0	0.0
35	1.5666186060058895	0.0	0.0	0.0	0.0
36	1.6203370189053572	0.0	0.0	0.0	0.0
37	1.6691059784214197	0.0	0.0	0.0	0.0
38	1.7123035637100896	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCAAT	15	1.4398679E-4	139.37811	44
TCGCTAG	60	0.0	139.37811	44
GCGATAC	45	0.0	139.37811	44
ATACGAG	120	0.0	139.37811	44
AAATCGA	85	0.0	131.1794	44
CGATTCA	65	0.0	128.65671	44
TCGTAGG	160	0.0	126.31142	44
CGAACCG	150	0.0	125.44031	44
TAAGCCA	3985	0.0	122.06514	44
ACACGGC	70	0.0	119.46694	44
GTAACGA	40	6.339178E-9	104.533585	44
CTATCAT	2875	0.0	103.98818	44
TAACACC	140	0.0	84.62242	44
ATACTGC	150	0.0	83.62687	44
GGATATC	145	0.0	81.70441	44
GAACACA	2350	0.0	80.95793	44
ACATTGG	850	0.0	73.78841	44
CGACGGG	1165	0.0	72.380905	44
CTATATA	165	0.0	71.80084	44
TTGTACA	165	0.0	71.80084	44
>>END_MODULE