FastQCFastQC Report
Tue 24 May 2016
ERR840907.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840907.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062741
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA315670.7769877528496155No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT224360.552238008772895No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT151120.3719656261622387No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC129690.31921798608377944No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA97830.24079802281267745No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG83500.20552626908779073No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG72070.17739255345098295No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC70410.1733066419936688No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT68430.1684330849542218No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC67060.16506097730571553No Hit
AATTCGTGGAGAAAGAAATGGCTCC64210.15804600883984482No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG57400.1412839262950801No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA57330.14111162882398853No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT54340.1337520654159347No Hit
AGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATG51690.12722937543889704No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG47210.11620233728903712No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG44190.10876893210765835No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA44160.10869509033433339No Hit
AGCCTAGGCCCTTCCTCGCTTTTCCAAGTCTTTTGCCAACCAGTTTGTT43620.1073659384144842No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG42670.10502761559252731No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG42630.10492915989476069No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT42080.10357539405046985No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC41390.1018770332639959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC256.8482586E-788.92815444
TGTACGA850.073.23495544
ATAGTCC257.3188494E-571.14252544
TAAGCCA41900.070.46335644
TAGACGC1100.064.67502644
TCGACAT1050.063.52011544
TAGTCGT950.060.84558544
AAAAACG751.8189894E-1259.2854444
ATTCGTC900.059.2023243
TATTAGG850.059.20231643
ATAACGC850.057.54174844
TAGAACG2350.056.76265344
CATGCTA1500.055.255543
TGTACGG1650.053.89584744
GCGTTTA1000.053.2820943
TCGGAAT1600.052.80109444
CGTTAGT503.6379788E-1252.64478742
CCCGTTA1050.052.64478742
AATCTCG650.052.64478742
ATAGACC700.052.64478342
Produced by FastQC (version 0.11.5)