##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840907.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4062741
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.42153659315226	39.0	35.0	39.0	31.0	39.0
2	37.087796883926394	39.0	37.0	39.0	32.0	39.0
3	37.08768316759547	39.0	37.0	39.0	33.0	39.0
4	37.41028876810016	39.0	37.0	39.0	33.0	40.0
5	37.95659925158901	39.0	37.0	40.0	33.0	41.0
6	37.94222521199358	39.0	37.0	40.0	33.0	41.0
7	37.891787096445476	39.0	37.0	40.0	33.0	41.0
8	37.79319331456276	39.0	37.0	40.0	33.0	41.0
9	37.59946942224474	39.0	36.0	40.0	33.0	41.0
10	37.53169522743389	39.0	36.0	40.0	33.0	41.0
11	37.40697548773107	39.0	36.0	40.0	32.0	41.0
12	37.577225818726816	39.0	36.0	40.0	33.0	41.0
13	37.773491098743435	39.0	37.0	40.0	33.0	41.0
14	37.784477031639476	39.0	36.0	40.0	33.0	41.0
15	37.748028732326276	39.0	36.0	41.0	33.0	41.0
16	37.71847307027448	39.0	36.0	41.0	33.0	41.0
17	37.65997069466156	39.0	36.0	40.0	33.0	41.0
18	37.64437481985684	39.0	36.0	41.0	33.0	41.0
19	37.553370495436454	39.0	36.0	41.0	33.0	41.0
20	37.50719403476619	39.0	35.0	41.0	33.0	41.0
21	37.36732171703783	39.0	35.0	40.0	32.0	41.0
22	37.27599027356162	39.0	35.0	40.0	32.0	41.0
23	37.130244827322244	39.0	35.0	40.0	32.0	41.0
24	36.914124725154764	38.0	35.0	40.0	31.0	41.0
25	36.74406533623079	38.0	35.0	40.0	31.0	41.0
26	36.64439406187531	38.0	35.0	40.0	31.0	41.0
27	36.57722419101313	38.0	35.0	40.0	31.0	41.0
28	36.531111536993215	38.0	35.0	40.0	31.0	41.0
29	36.41820228097417	38.0	34.0	40.0	31.0	41.0
30	36.35800965422443	37.0	34.0	40.0	31.0	41.0
31	36.26377579220072	37.0	34.0	40.0	31.0	41.0
32	36.13208689870452	37.0	34.0	40.0	30.0	41.0
33	36.05411822330404	37.0	34.0	40.0	30.0	41.0
34	35.97186745973473	37.0	34.0	40.0	31.0	41.0
35	35.88839200781867	36.0	34.0	40.0	30.0	41.0
36	35.79293007654748	36.0	34.0	40.0	30.0	41.0
37	35.72314352220375	36.0	34.0	39.0	30.0	41.0
38	35.59604867696549	36.0	34.0	39.0	30.0	41.0
39	35.49278658365432	35.0	34.0	39.0	30.0	41.0
40	35.366955207071484	35.0	34.0	39.0	30.0	41.0
41	35.3028081208519	35.0	34.0	39.0	30.0	41.0
42	35.17045949678328	35.0	33.0	39.0	30.0	41.0
43	35.10852306416058	35.0	34.0	39.0	30.0	41.0
44	34.961680057132654	35.0	33.0	38.0	30.0	40.0
45	34.79712993917882	35.0	33.0	38.0	29.0	40.0
46	34.65809548976808	35.0	33.0	38.0	29.0	40.0
47	34.56478467058734	35.0	33.0	37.0	29.0	40.0
48	34.46677436177355	35.0	33.0	37.0	29.0	40.0
49	34.48633050878893	35.0	33.0	37.0	29.0	40.0
50	34.721733351383946	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	103.0
14	1203.0
15	2835.0
16	330.0
17	6.0
18	1.0
19	5.0
20	11.0
21	44.0
22	101.0
23	270.0
24	702.0
25	1640.0
26	3981.0
27	9060.0
28	20042.0
29	39859.0
30	72898.0
31	123877.0
32	193524.0
33	285426.0
34	379378.0
35	443887.0
36	537429.0
37	564082.0
38	557606.0
39	554567.0
40	269874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.1147668532156	15.09441532206951	32.79081782471489
2	27.618127761528484	23.229637331053098	28.91518312390576	20.23705178351266
3	24.81278033721569	18.92933859185215	28.02834342627305	28.229537644659107
4	22.07005073668245	19.40177825758521	32.91903175713146	25.609139248600883
5	19.040741213875066	22.601834574244332	31.80475939765789	26.552664814222716
6	23.23679998306562	26.416008305722666	26.483105863750605	23.864085847461112
7	28.371313849443027	25.761450213045823	21.599358659584748	24.267877277926406
8	25.68962678152508	25.074844790745953	24.1670586434134	25.068469784315567
9	24.03050059061112	24.020015058798975	27.774229270337443	24.17525508025247
10	27.054444277890223	22.113641996868616	24.63681539138232	26.19509833385884
11	24.797199723044123	24.21397278339919	25.4159691695828	25.57285832397389
12	26.198741194676206	22.895257167513265	26.9116589022042	23.99434273560633
13	24.352327652685712	22.761431260324002	26.754498994644255	26.131742092346027
14	23.07075444878224	24.281414936369313	26.255771657607514	26.392058957240938
15	22.765935608496825	25.903546398847478	26.639551967501745	24.690966025153955
16	26.518180706079956	28.693928557099753	22.119130902019105	22.668759834801186
17	25.759874921881558	28.76678577344704	20.156785775908432	25.31655352876297
18	24.415536210651872	24.317523563525217	22.618941251731282	28.64799897409163
19	24.380855191113586	24.491297870083276	24.45895517336695	26.668891765436193
20	26.702637455845696	22.79325706462706	24.2752614552589	26.228844024268344
21	25.733193427786805	26.975359738659193	21.015294846508798	26.2761519870452
22	25.758078105397313	25.781879770332395	22.87445840136007	25.585583722910222
23	27.408810948076678	24.773865722673435	22.933261066851173	24.88406226239871
24	26.664412031187812	26.484238104274922	22.49523166748754	24.356118197049724
25	26.69150672198412	26.981732181733275	23.47725354855864	22.849507547723967
26	27.048621685810794	24.78609340828054	24.156963008917213	24.008321896991458
27	25.099122675542613	26.009026753960168	23.783755229736876	25.10809534076034
28	27.169496942058913	24.01828087592848	24.992622102295634	23.819600079716974
29	27.46091288392074	24.25192861349885	25.44686067440721	22.840297828173203
30	25.70199027826714	25.751790725951874	23.947861683540744	24.59835731224024
31	25.07257310355125	24.899607861627807	25.858185790288484	24.16963324453246
32	23.145674048630955	25.29705436685596	27.165065893165995	24.39220569134709
33	25.24852970672119	24.308786559699342	25.70968140819123	24.733002325388235
34	24.443803762807086	25.269842980606654	25.47057991418159	24.81577334240467
35	23.554789970962624	25.471816569893534	24.377588060649085	26.595805398494754
36	23.081680250618707	24.81348336536975	27.544674626610366	24.56016175740118
37	23.539036991354013	26.429822617358877	25.917908339620897	24.113232051666213
38	24.49413550561867	26.491893457751253	23.84794747585245	25.166023560777628
39	24.78280278255929	26.957367683579136	24.209049292242	24.050780241619574
40	22.139349891152925	27.481643538146606	24.849603618912475	25.529402951787993
41	24.88281036481693	25.879102068763142	23.916783419975314	25.32130414644461
42	22.96562487010607	27.183911203273432	24.76767335008184	25.082790576538667
43	24.1570254274731	26.097678488961556	25.678370200655948	24.066925882909395
44	23.754389097185026	27.452835803130395	24.489168600845087	24.303606498839493
45	25.653100795632085	24.739442168651102	27.06266977426708	22.544787261449734
46	23.64319570102052	26.528112765726696	24.783256220337975	25.04543531291481
47	23.894126279635813	26.139056214775596	24.65113392913932	25.31568357644927
48	22.35131540507711	25.586653238413003	24.808122043349684	27.2539093131602
49	23.204300308528268	26.79143208326626	23.99490624046766	26.00936136773782
50	22.341435955006876	29.78918771694851	24.290313829215133	23.579062498829476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23963.0
1	18075.5
2	10470.0
3	7681.0
4	6254.5
5	5554.0
6	4755.5
7	4142.0
8	4040.5
9	4681.5
10	5283.5
11	5466.5
12	5611.0
13	6126.0
14	7256.5
15	8471.5
16	8922.0
17	9445.5
18	10527.0
19	14047.0
20	17472.5
21	17585.0
22	18172.0
23	22116.5
24	24579.5
25	32956.5
26	43200.5
27	43784.5
28	42967.5
29	51193.0
30	65049.0
31	79842.5
32	90579.0
33	99578.5
34	112418.5
35	128810.0
36	140118.0
37	140043.5
38	143954.0
39	172589.0
40	200337.0
41	201846.0
42	209824.5
43	228910.5
44	243055.5
45	243552.5
46	243106.0
47	239240.5
48	235717.5
49	256630.5
50	287406.0
51	287465.0
52	275682.5
53	275802.0
54	271110.0
55	261222.5
56	256775.5
57	267302.5
58	269929.0
59	259034.0
60	252115.0
61	234816.0
62	212072.0
63	204920.5
64	194876.0
65	167375.5
66	135947.5
67	118440.0
68	108990.0
69	97248.5
70	81996.0
71	68256.0
72	61732.0
73	57150.0
74	51287.0
75	46800.5
76	43131.5
77	33572.0
78	22942.0
79	18318.5
80	14303.0
81	10009.0
82	7519.0
83	6136.5
84	5392.0
85	4580.0
86	3577.5
87	2689.0
88	1666.5
89	1094.5
90	746.5
91	423.5
92	212.5
93	72.0
94	42.0
95	13.0
96	13.0
97	13.0
98	6.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	34838.0
25	38428.0
26	33014.0
27	32579.0
28	39226.0
29	33285.0
30	33841.0
31	38003.0
32	35206.0
33	32996.0
34	31929.0
35	31464.0
36	33655.0
37	34514.0
38	31375.0
39	37499.0
40	34899.0
41	35683.0
42	38514.0
43	41193.0
44	41855.0
45	45410.0
46	50250.0
47	101309.0
48	345045.0
49	907912.0
50	1868819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.142170205362197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.61567634557815	6.181540217019581
2	13.220138882164148	2.4172151959854276
3	5.59519829003089	1.5345676530064172
4	2.896738518653423	1.0592990631183339
5	1.7716417570657739	0.8098325243011123
6	1.1922891712320867	0.6540066322448381
7	0.853050600394263	0.5459113647813535
8	0.6302045909144977	0.4609150107472793
9	0.4887362514126037	0.4021298996350242
>10	3.653119956079403	6.730833649640624
>50	0.5836876499324544	3.7505163029864104
>100	0.8063775652811338	18.26094869681994
>500	0.5804412219952192	38.71275581075387
>1k	0.10866462172663047	14.630943923504825
>5k	0.0029586901955270676	1.8301497370494635
>10k+	0.0010758873438280244	2.018434318405527
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	31567	0.7769877528496155	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	22436	0.552238008772895	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	15112	0.3719656261622387	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12969	0.31921798608377944	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9783	0.24079802281267745	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	8350	0.20552626908779073	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	7207	0.17739255345098295	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC	7041	0.1733066419936688	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	6843	0.1684330849542218	No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC	6706	0.16506097730571553	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	6421	0.15804600883984482	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	5740	0.1412839262950801	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	5733	0.14111162882398853	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	5434	0.1337520654159347	No Hit
AGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATG	5169	0.12722937543889704	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG	4721	0.11620233728903712	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	4419	0.10876893210765835	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	4416	0.10869509033433339	No Hit
AGCCTAGGCCCTTCCTCGCTTTTCCAAGTCTTTTGCCAACCAGTTTGTT	4362	0.1073659384144842	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	4267	0.10502761559252731	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4263	0.10492915989476069	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	4208	0.10357539405046985	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	4139	0.1018770332639959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.019445000308904753	0.0	0.0	0.0	0.0
2	0.038643861373392985	0.0	0.0	0.0	0.0
3	0.05712891862907333	0.0	0.0	0.0	0.0
4	0.07903531138214324	0.0	0.0	0.0	0.0
5	0.10273852061945371	0.0	0.0	0.0	0.0
6	0.1435730212681537	0.0	0.0	0.0	0.0
7	0.18172460415271366	0.0	0.0	0.0	0.0
8	0.22868797198738486	0.0	0.0	0.0	0.0
9	0.27205770685357494	0.0	0.0	0.0	0.0
10	0.31786422023948857	0.0	0.0	0.0	0.0
11	0.36876581598482405	0.0	0.0	0.0	0.0
12	0.4179444370192439	0.0	0.0	0.0	0.0
13	0.46778763401358836	0.0	0.0	0.0	0.0
14	0.5232674197050711	0.0	0.0	0.0	0.0
15	0.5846791611869917	0.0	0.0	0.0	0.0
16	0.6451555735401296	0.0	0.0	0.0	0.0
17	0.7115885556081473	0.0	0.0	0.0	0.0
18	0.7677821451084379	0.0	0.0	0.0	0.0
19	0.8323936967677733	0.0	0.0	0.0	0.0
20	0.9013126852043977	0.0	0.0	0.0	0.0
21	0.9625521292152268	0.0	0.0	0.0	0.0
22	1.0213301807818909	0.0	0.0	0.0	0.0
23	1.0733394031271006	0.0	0.0	0.0	0.0
24	1.1318959293737898	0.0	0.0	0.0	0.0
25	1.1884341138162635	0.0	0.0	0.0	0.0
26	1.2414032792146976	0.0	0.0	0.0	0.0
27	1.2941263053687153	0.0	0.0	0.0	0.0
28	1.347489293558216	0.0	0.0	0.0	0.0
29	1.4103532565821941	0.0	0.0	0.0	0.0
30	1.4645285042782692	0.0	0.0	0.0	0.0
31	1.5205497963074683	0.0	0.0	0.0	0.0
32	1.572214423710495	0.0	0.0	0.0	0.0
33	1.6256020258244372	0.0	0.0	0.0	0.0
34	1.6859799824798085	0.0	0.0	0.0	0.0
35	1.7452010846864223	0.0	0.0	0.0	0.0
36	1.80597286413286	0.0	0.0	0.0	0.0
37	1.863766358721858	0.0	0.0	0.0	0.0
38	1.916046334235926	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	25	6.8482586E-7	88.928154	44
TGTACGA	85	0.0	73.234955	44
ATAGTCC	25	7.3188494E-5	71.142525	44
TAAGCCA	4190	0.0	70.463356	44
TAGACGC	110	0.0	64.675026	44
TCGACAT	105	0.0	63.520115	44
TAGTCGT	95	0.0	60.845585	44
AAAAACG	75	1.8189894E-12	59.28544	44
ATTCGTC	90	0.0	59.20232	43
TATTAGG	85	0.0	59.202316	43
ATAACGC	85	0.0	57.541748	44
TAGAACG	235	0.0	56.762653	44
CATGCTA	150	0.0	55.2555	43
TGTACGG	165	0.0	53.895847	44
GCGTTTA	100	0.0	53.28209	43
TCGGAAT	160	0.0	52.801094	44
CGTTAGT	50	3.6379788E-12	52.644787	42
CCCGTTA	105	0.0	52.644787	42
AATCTCG	65	0.0	52.644787	42
ATAGACC	70	0.0	52.644783	42
>>END_MODULE