##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840906.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16924837
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.01334246232327	39.0	37.0	39.0	33.0	39.0
2	37.55024163600512	39.0	37.0	39.0	35.0	39.0
3	36.96734308283146	39.0	37.0	39.0	33.0	39.0
4	37.573111989202616	39.0	37.0	39.0	34.0	40.0
5	38.39697221308542	40.0	38.0	41.0	34.0	41.0
6	38.22621405452826	40.0	37.0	41.0	34.0	41.0
7	38.471219663740335	40.0	38.0	41.0	34.0	41.0
8	38.09490064808305	39.0	37.0	41.0	33.0	41.0
9	38.374139319628306	40.0	38.0	41.0	34.0	41.0
10	38.315619228711036	40.0	38.0	41.0	34.0	41.0
11	38.33743515521006	40.0	38.0	41.0	34.0	41.0
12	38.46230944498904	40.0	38.0	41.0	34.0	41.0
13	38.7160537498825	40.0	38.0	41.0	35.0	41.0
14	38.71627602676469	40.0	38.0	41.0	35.0	41.0
15	38.69214391843183	40.0	38.0	41.0	35.0	41.0
16	38.771957862873364	40.0	38.0	41.0	35.0	41.0
17	38.7381448341275	40.0	38.0	41.0	35.0	41.0
18	38.753517389857286	40.0	38.0	41.0	35.0	41.0
19	38.66924691800577	40.0	38.0	41.0	35.0	41.0
20	38.6489713313044	40.0	38.0	41.0	34.0	41.0
21	38.456667263619735	40.0	38.0	41.0	34.0	41.0
22	38.320222699929104	40.0	38.0	41.0	34.0	41.0
23	37.91648776292499	40.0	37.0	41.0	33.0	41.0
24	37.779856609549626	40.0	37.0	41.0	33.0	41.0
25	37.6854101528629	40.0	36.0	41.0	33.0	41.0
26	37.71602207355325	40.0	36.0	41.0	33.0	41.0
27	37.7663449099957	40.0	36.0	41.0	33.0	41.0
28	37.772691840685304	40.0	36.0	41.0	33.0	41.0
29	37.741934236921864	40.0	36.0	41.0	33.0	41.0
30	37.69178921465466	39.0	36.0	41.0	33.0	41.0
31	37.50421522542	39.0	35.0	41.0	33.0	41.0
32	37.291358558279946	39.0	35.0	41.0	32.0	41.0
33	37.13705773717634	39.0	35.0	41.0	32.0	41.0
34	36.99279387479358	38.0	35.0	40.0	32.0	41.0
35	36.8680906618835	38.0	35.0	40.0	32.0	41.0
36	36.76487651782525	38.0	35.0	40.0	31.0	41.0
37	36.699582427698566	38.0	35.0	40.0	31.0	41.0
38	36.58362275932047	38.0	35.0	40.0	31.0	41.0
39	36.479329591970306	38.0	35.0	40.0	31.0	41.0
40	36.398865594684096	38.0	35.0	40.0	31.0	41.0
41	36.31510895379678	37.0	35.0	40.0	31.0	41.0
42	36.217717924910666	37.0	34.0	40.0	31.0	41.0
43	36.15354403328794	37.0	34.0	40.0	31.0	41.0
44	36.05074474980906	37.0	34.0	40.0	30.0	41.0
45	35.92210694118482	37.0	34.0	40.0	30.0	41.0
46	35.75648730419183	37.0	34.0	40.0	29.0	41.0
47	35.61512747286806	36.0	34.0	40.0	29.0	41.0
48	35.56803208995111	36.0	34.0	40.0	29.0	41.0
49	35.64882553798532	37.0	34.0	40.0	29.0	41.0
50	35.948676362412876	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	25.0
14	833.0
15	12569.0
16	23682.0
17	1231.0
18	1.0
19	12.0
20	52.0
21	142.0
22	301.0
23	713.0
24	1553.0
25	3399.0
26	8079.0
27	19144.0
28	43058.0
29	89416.0
30	170495.0
31	295291.0
32	474788.0
33	725463.0
34	1051573.0
35	1337081.0
36	1936306.0
37	2473078.0
38	2614316.0
39	2832175.0
40	2810061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	58.497951856198085	14.745170071652684	26.75687807214923
2	39.39041776295984	25.537646241437955	21.850473360541077	13.221462635061124
3	22.975027765407727	17.074959126637378	35.81041282701866	24.139600280936236
4	32.68876385633729	22.871245377429634	24.304245884317822	20.135744881915258
5	21.603569948709104	20.925832254691727	23.93712270316104	33.53347509343813
6	20.13549081743003	35.894472720771255	27.050476172975845	16.919560288822872
7	42.6020173783653	19.635503727450963	19.125294973298708	18.63718392088503
8	19.9756251714566	21.237533927210052	34.02729373405487	24.759547167278477
9	35.12784199930552	22.617281336298838	25.434868294447977	16.82000836994767
10	23.508604543724704	19.99743335785154	23.32355697133154	33.17040512709221
11	20.98084016998214	21.13128770457287	39.66502602063465	18.222846104810344
12	25.595832917031935	32.49000271021812	22.75960471583862	19.154559656911317
13	18.694679304740127	17.24202720534325	36.87634923751407	27.18694425240255
14	32.21824233816846	22.595213176942266	25.42596422051214	19.76058026437714
15	17.201512782663727	24.826874255864325	25.512866091413468	32.458746870058484
16	24.829993931403887	21.675027062299034	34.07814799043559	19.416831015861483
17	27.106636241164388	22.256811099569234	29.756304300005958	20.880248359260417
18	34.72379674912084	20.539128382743066	18.96656966327061	25.77050520486549
19	33.38208220262328	23.656730047089965	24.08342839579489	18.877759354491864
20	38.63278565105235	20.527388240134897	22.41633996238782	18.423486146424924
21	36.04562336405367	25.581368966803048	19.052927954343076	19.320079714800205
22	33.088495918749466	28.298588636333694	19.784911370195175	18.828004074721665
23	29.364460053588697	30.77231999339196	21.480307314037944	18.382912638981395
24	26.36879161672281	33.97623858947652	19.224988695607525	20.42998109819315
25	22.606235174901734	38.0434833905434	19.817355924403028	19.53292551015184
26	22.122042389834498	38.57751211129092	20.70086296694142	18.599582531933162
27	21.679072114014115	39.19636128479736	19.860528635367576	19.264037965820943
28	21.602216375782056	38.16745300612638	21.22366887596886	19.006661742122706
29	22.5454193479356	38.5186005252808	20.148405896776417	18.787574230007184
30	21.9016152895143	38.11820614176289	20.29435087337948	19.68582769534333
31	20.243747025226458	38.466116366443934	21.80549173773057	19.48464487059904
32	20.504699614721268	37.778387570950514	22.720354941875478	18.996557872452748
33	21.194288821069478	37.02183390950896	21.96367827660234	19.82019899281922
34	21.205203361213197	37.38707010548007	22.57820902904455	18.829517504262185
35	20.72241838060009	36.69546008771086	21.177205379925983	21.404916151763064
36	20.269692331123526	37.35054378147936	22.995803773797657	19.38396011359945
37	20.25369089754491	37.74077778926955	21.123560820364958	20.881970492820585
38	20.81203304949349	38.1559189749465	20.90763883225761	20.1244091433024
39	21.691134077036526	38.34580827660318	20.702763499857944	19.260294146502343
40	20.52414034281932	38.579624798623406	21.47412593364738	19.422108924909892
41	22.337379824824342	36.73779516378946	21.64766968096514	19.277155330421063
42	20.749111673025524	37.79436955422219	21.78094527897453	19.675573493777744
43	21.049957818041218	35.98435843146278	22.600466836861248	20.365216913634754
44	20.944508943967268	36.06961615060026	22.56521684177836	20.420658063654106
45	20.702886688499053	36.64402467293398	22.23168154841249	20.42140709015448
46	20.946154486061193	36.40467685096215	22.43935567914526	20.209812983831394
47	22.361343909461066	34.22214698365326	23.10148398014507	20.315025126740608
48	21.143544853713856	33.80026591256302	22.187483259632078	22.868705974091046
49	20.72043849304191	33.56921849152393	22.28263875234189	23.427704263092274
50	20.144703899299703	34.856935515261064	24.453177726392596	20.545182859046633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181964.0
1	176027.0
2	112118.0
3	53652.5
4	52489.5
5	41802.0
6	23774.5
7	15763.5
8	15457.0
9	15495.0
10	15676.0
11	15582.0
12	16133.5
13	19940.0
14	25983.0
15	35191.0
16	44615.0
17	58323.5
18	74885.0
19	97344.5
20	121322.0
21	169972.0
22	233977.5
23	333504.5
24	442569.5
25	530557.0
26	627223.0
27	692415.5
28	700420.0
29	682233.0
30	666736.5
31	656470.0
32	645229.0
33	639889.0
34	633131.0
35	624993.0
36	635350.5
37	650600.5
38	659808.0
39	672364.5
40	712187.5
41	765076.0
42	813430.5
43	936550.0
44	1047069.5
45	970852.0
46	895663.5
47	896412.0
48	969013.5
49	1077162.0
50	1166738.5
51	1254479.0
52	1263893.5
53	1210332.5
54	1138389.5
55	1027508.5
56	909732.0
57	812130.5
58	734156.5
59	673968.0
60	622737.0
61	580841.0
62	534864.5
63	503678.0
64	471923.5
65	426329.0
66	380603.5
67	338473.5
68	298241.5
69	266121.5
70	229125.5
71	195878.5
72	172435.0
73	151009.0
74	136097.5
75	121993.5
76	105428.0
77	80516.0
78	59401.0
79	53095.5
80	47078.5
81	35574.5
82	26174.0
83	21082.5
84	16239.5
85	12997.0
86	10072.0
87	7256.0
88	4864.5
89	3508.5
90	2571.0
91	1793.5
92	1291.5
93	1115.0
94	692.5
95	235.0
96	194.5
97	205.5
98	268.5
99	316.5
100	303.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	185066.0
25	140178.0
26	140157.0
27	145235.0
28	145706.0
29	131308.0
30	134716.0
31	122986.0
32	112387.0
33	107002.0
34	115943.0
35	119431.0
36	125438.0
37	153123.0
38	164597.0
39	172183.0
40	194008.0
41	202794.0
42	222432.0
43	244222.0
44	267321.0
45	307452.0
46	352246.0
47	505051.0
48	1148910.0
49	3686020.0
50	7578925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.530780785018258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.92748417236336	7.258602258819743
2	13.024835562235642	2.7432337613362714
3	5.461478219780084	1.7254088968396752
4	2.855211293268752	1.2027041689728684
5	1.7104366264199362	0.9006116579747261
6	1.118367116651654	0.7066367365578907
7	0.8064102953951211	0.5944491030511487
8	0.5991970795498504	0.5048010473410097
9	0.4696667245567311	0.4451361586492038
>10	3.3437262873377818	7.160160323393907
>50	0.5239929185136646	3.8760248580541923
>100	0.6448311511309779	16.190796174269895
>500	0.42400046771598504	32.383411338399
>1k	0.08249487718561695	14.316451281929766
>5k	0.004487632672511764	3.1829032266215536
>10k+	0.003379575222508859	6.80866900778936
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	167205	0.987926796577125	No Hit
AAGCAGTGGTATCAACGCAGAGTG	44514	0.2630099185002491	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41082	0.24273202749308603	No Hit
AGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	35699	0.2109266990281797	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	30768	0.1817920018963846	No Hit
AGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA	30685	0.18130159835512744	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	30179	0.17831190929637905	No Hit
AGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	26055	0.15394535261993955	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	22801	0.13471917041209908	No Hit
CAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	22520	0.13305888854350562	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	21411	0.12650638821514204	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	21142	0.12491700806335684	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	20253	0.11966437254314474	No Hit
AGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20139	0.11899080623346624	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	19645	0.11607201889152612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	19439	0.11485487275298427	No Hit
AGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	18138	0.10716794495568849	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAGCAGTGGT	17569	0.10380602188369671	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	17232	0.1018148653366647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16989	0.10037910557129737	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.16140184983760847	0.0	0.0	0.0	0.0
2	0.25713689295796466	0.0	0.0	0.0	0.0
3	0.3241271983889712	0.0	0.0	0.0	0.0
4	0.4252684974159574	0.0	0.0	0.0	0.0
5	0.501286954787216	0.0	0.0	0.0	0.0
6	0.7334959858106758	0.0	0.0	0.0	0.0
7	0.8348322645588847	0.0	0.0	0.0	0.0
8	0.9693505467733604	0.0	0.0	0.0	0.0
9	1.1126606418720606	0.0	0.0	0.0	0.0
10	1.2331522011113016	0.0	0.0	0.0	0.0
11	1.351516708846295	0.0	0.0	0.0	0.0
12	1.6839453165782334	0.0	0.0	0.0	5.908476400688526E-6
13	1.794262479455489	0.0	0.0	0.0	5.908476400688526E-6
14	1.9599302492543946	0.0	0.0	0.0	5.908476400688526E-6
15	2.1173143351395347	0.0	0.0	0.0	5.908476400688526E-6
16	2.2277142166864	0.0	0.0	0.0	5.908476400688526E-6
17	2.4284842447817963	0.0	0.0	0.0	5.908476400688526E-6
18	2.5654368192733554	0.0	0.0	0.0	5.908476400688526E-6
19	2.7212492504359127	0.0	0.0	0.0	5.908476400688526E-6
20	2.8504853547481726	0.0	0.0	0.0	5.908476400688526E-6
21	3.023296472515511	0.0	0.0	0.0	5.908476400688526E-6
22	3.214406141695781	0.0	0.0	0.0	5.908476400688526E-6
23	3.4282220856838976	0.0	0.0	0.0	5.908476400688526E-6
24	3.6884491117994225	0.0	0.0	0.0	5.908476400688526E-6
25	3.9590100631397513	0.0	0.0	0.0	5.908476400688526E-6
26	4.255781015793534	0.0	0.0	0.0	5.908476400688526E-6
27	4.510838125058457	0.0	0.0	0.0	5.908476400688526E-6
28	4.753871484847978	0.0	0.0	0.0	5.908476400688526E-6
29	5.009330370508147	0.0	0.0	0.0	5.908476400688526E-6
30	5.251306113021945	0.0	0.0	0.0	5.908476400688526E-6
31	5.499987976250525	0.0	0.0	0.0	5.908476400688526E-6
32	5.751653620061452	0.0	0.0	0.0	5.908476400688526E-6
33	6.003809667413636	0.0	0.0	0.0	5.908476400688526E-6
34	6.240934550802469	0.0	0.0	0.0	5.908476400688526E-6
35	6.457840627948145	0.0	0.0	0.0	5.908476400688526E-6
36	6.654409729322651	0.0	0.0	0.0	5.908476400688526E-6
37	6.822464523587436	0.0	0.0	0.0	5.908476400688526E-6
38	6.967251737786308	0.0	0.0	0.0	5.908476400688526E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	20105	0.0	78.93542	44
CCGATTA	120	0.0	71.60857	44
TTAAGCC	20915	0.0	52.775627	43
TAATGCG	200	0.0	52.01043	44
ACCGATT	150	0.0	48.612263	43
ATTAAGC	20550	0.0	48.545223	42
CTATCAT	5805	0.0	47.135246	44
CGTACGT	165	0.0	46.92037	40
CGGGTAT	155	0.0	46.685375	44
CGAGTAT	60	0.0	46.54724	34
AATCGCA	1835	0.0	46.335556	44
TATACGG	80	6.528353E-9	45.22646	44
GATTAAG	21785	0.0	44.69735	41
TACGATA	100	0.0	43.025974	25
CGAGTTA	130	0.0	42.8247	41
ACCTTAG	5195	0.0	41.836033	42
GACGATA	270	0.0	41.74262	33
GTACGTT	100	0.0	41.289925	40
CGTTCGA	595	0.0	40.85064	43
AGATTAA	23365	0.0	40.83267	40
>>END_MODULE