FastQCFastQC Report
Tue 24 May 2016
ERR840905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10893147
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA10325989.479335953145588No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC593210.5445717385435082No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT519460.4768686220795515No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA370050.3397089931862665No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG362910.3331544135041967No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT335560.3080468848901057No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT334400.30698199519385905No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC314420.2886401881843695No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA276030.2533978472887587No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA238770.21919285583862957No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG235770.2164388307621296No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC230770.21184878896796308No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT189500.17396258399891235No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT179060.16437857673269257No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTGGTCTCAAAGATTAAGCCA175780.16136750931571933No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC174750.16042196070612103No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC171180.1571446708650861No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT168400.1545926076275295No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG166910.15322477517286787No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG164150.15069107210248794No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG156360.14353978698717643No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG154750.1420617935294548No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG150920.13854582151512324No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT144780.1329092501918867No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC144350.13251450659758837No Hit
CACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTT140800.1292555769237301No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC137440.1261710688380502No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG137270.12601500741704852No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG137190.12594156674834187No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG132210.12136988512135198No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG131640.120846620356817No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC130110.11944206756780204No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG128120.11761523093372374No Hit
CCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGAAG124930.1146867842690455No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC123320.11320879081132386No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA121860.11186849860742722No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC113480.10417558856040407No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT109800.1007973177998975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA1000.0114.31290444
TAAGCCA1142700.0109.7161644
CTATCAT89650.070.576944
ACTTCTA49550.064.13518544
TTAAGCC1144750.060.6236143
ACATTGG30250.057.62881544
AATCGCA14550.057.35286744
TAAGCGA2050.055.7623944
TTAAACG451.0986696E-955.31058543
CGTTATA700.053.33520543
GAACACA87100.052.56293544
GTTGCAA5500.051.9604144
CGACGGG84900.051.09745844
ATTAAGC1145250.050.86670342
GATTAAG1168950.048.6543241
TATACGG1200.048.60185239
AACGACA23750.047.94554543
AGCGTAC1450.047.61491840
CGTTAAT252.8047121E-547.56808537
AGATTAA1179050.047.5315540
Produced by FastQC (version 0.11.5)