FastQCFastQC Report
Tue 24 May 2016
ERR840904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2946418
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC342971.1640235703148705No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA187910.6377574397115413No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT100570.34132970949810926No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG85570.2904204359327156No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT83300.282716165866486No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT78910.2678167184696808No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA69340.23533660193495964No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG66020.22406868271915253No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT63370.215074711055933No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC62530.21222379173627096No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA57170.19403221131557027No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC55080.18693885253212544No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG54030.1833752033825479No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA53430.18133883243993215No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG52110.1768588163661775No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT50500.17139455433682527No Hit
ACTTGTGTTTGGGAACAGATCAGGACTCAGGATGCAAGACCCTGGTCAT50480.1713266753054047No Hit
AGCCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGA50380.1709872801483021No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT49890.16932424387849926No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG49150.16681271971593983No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT47860.16243452218931598No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG46970.1594139052911026No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT46780.15876905449260764No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG46400.15747935289561768No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT45480.15435691745027352No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTC41350.14033989746193512No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG41280.14010232085196328No Hit
TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC40520.13752291765798336No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC39700.13473987736974183No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG37860.12849500647905354No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT36860.1251010549080273No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG36390.12350589766964497No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36230.12296286541828078No Hit
ATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT34580.11736284532608747No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG32990.11196646232815574No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA31720.10765614383295241No Hit
TCCCAGATACCCGGTGTCAGCTGGTGAAAGAAGGACGCGATGAAGTTG31680.10752038577011137No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC30570.10375309952627224No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA30490.10348158340059013No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT30490.10348158340059013No Hit
CTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGCC30310.10287067211780543No Hit
AATTCGTGGAGAAAGAAATGGCTCC29940.10161491003652572No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT29830.10124157536371282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA151.2125449E-4145.5188944
ATGACGT500.0145.5188944
CTATACA750.0135.8176344
TCGTAAA1350.0134.7397244
CAATCCG600.0133.3923244
GCGATCC550.0132.289944
CGTCACA900.0113.18136644
GAACACA30750.0107.423744
CCGATAA1250.098.9528544
GGGCAAT607.2759576E-1297.01259644
AACGTAT1400.093.5478644
CGGCAAA1000.087.3113344
ATCACCG2200.085.9884444
GATCAGT1200.084.88602444
AATCGCA2650.082.3691944
GGCATAA1600.081.8543844
CTATCAT14150.081.2437744
CCGCCTA2150.077.8356944
CTAACCT1650.070.5546144
CCCGTAT1250.069.84907544
Produced by FastQC (version 0.11.5)