##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840904.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2946418 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64126610684567 39.0 37.0 39.0 32.0 39.0 2 37.26336758735522 39.0 37.0 39.0 34.0 39.0 3 37.52946289358808 39.0 37.0 39.0 34.0 40.0 4 37.949102605265104 39.0 37.0 40.0 34.0 41.0 5 38.39287297321697 40.0 38.0 41.0 34.0 41.0 6 38.390030878171395 40.0 38.0 41.0 34.0 41.0 7 38.34036379088099 40.0 38.0 41.0 34.0 41.0 8 38.220245056879236 40.0 37.0 41.0 33.0 41.0 9 38.08034433675059 40.0 37.0 41.0 33.0 41.0 10 38.0466400218842 40.0 37.0 41.0 33.0 41.0 11 38.03630781511652 39.0 37.0 41.0 33.0 41.0 12 38.19332016027597 40.0 37.0 41.0 34.0 41.0 13 38.23728167558031 40.0 37.0 41.0 34.0 41.0 14 38.251242016577415 40.0 37.0 41.0 34.0 41.0 15 38.23243171878532 40.0 37.0 41.0 34.0 41.0 16 38.21028177264733 40.0 37.0 41.0 34.0 41.0 17 38.211339327956864 40.0 37.0 41.0 34.0 41.0 18 38.249565065106175 40.0 37.0 41.0 34.0 41.0 19 38.221061302232066 40.0 37.0 41.0 34.0 41.0 20 38.15946786912108 40.0 37.0 41.0 33.0 41.0 21 38.06862637955646 40.0 37.0 41.0 33.0 41.0 22 37.968508880953074 40.0 37.0 41.0 33.0 41.0 23 37.808610658772785 40.0 36.0 41.0 33.0 41.0 24 37.65332176222111 39.0 36.0 41.0 33.0 41.0 25 37.54948341517855 39.0 35.0 41.0 32.0 41.0 26 37.387562966583026 39.0 35.0 41.0 32.0 41.0 27 37.33360881801747 39.0 35.0 41.0 32.0 41.0 28 37.294063502792625 39.0 35.0 41.0 32.0 41.0 29 37.26563204662481 39.0 35.0 41.0 32.0 41.0 30 37.18152199541301 39.0 35.0 41.0 32.0 41.0 31 37.0610560651307 39.0 35.0 41.0 31.0 41.0 32 36.940135997451186 39.0 35.0 41.0 31.0 41.0 33 36.86826023709452 38.0 35.0 40.0 31.0 41.0 34 36.853212801457886 38.0 35.0 40.0 31.0 41.0 35 36.66478514379809 38.0 35.0 40.0 31.0 41.0 36 36.630482166821025 38.0 35.0 40.0 31.0 41.0 37 36.56564379736889 38.0 35.0 40.0 31.0 41.0 38 36.48880778363923 38.0 35.0 40.0 31.0 41.0 39 36.339811456393164 37.0 34.0 40.0 31.0 41.0 40 36.267212070866165 37.0 34.0 40.0 31.0 41.0 41 36.2168668824412 37.0 34.0 40.0 31.0 41.0 42 36.08631219356837 37.0 34.0 40.0 30.0 41.0 43 36.01268343170846 37.0 34.0 40.0 30.0 41.0 44 35.817626719153445 36.0 34.0 40.0 30.0 41.0 45 35.66765861567697 36.0 34.0 40.0 30.0 41.0 46 35.490827964202985 36.0 34.0 39.0 30.0 41.0 47 35.46206880835717 36.0 34.0 39.0 30.0 41.0 48 35.38403774528229 36.0 34.0 39.0 29.0 41.0 49 35.717717535580405 36.0 34.0 40.0 30.0 41.0 50 36.41724977032981 38.0 34.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 8.0 14 333.0 15 2822.0 16 2092.0 17 34.0 18 0.0 19 1.0 20 9.0 21 41.0 22 99.0 23 218.0 24 521.0 25 1135.0 26 2433.0 27 5111.0 28 10249.0 29 20131.0 30 37048.0 31 64918.0 32 103851.0 33 158332.0 34 226816.0 35 238575.0 36 315282.0 37 386181.0 38 429799.0 39 497385.0 40 442994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 43.62452985285862 14.676736294714463 41.69873385242691 2 26.648561066352432 21.12728065060694 29.235668530398605 22.98848975264202 3 24.78304164582215 18.94941586699511 27.392209795079992 28.875332692102752 4 19.875998585400986 20.133327993516197 33.39383617667283 26.596837244409993 5 20.515419061382328 24.671346699619672 30.483183309360722 24.330050929637277 6 24.862460112584163 26.026755199024716 25.716548025432918 23.39423666295821 7 29.13527544292765 23.77700652113855 21.94708965258833 25.14062838334547 8 26.193364281646392 21.758962917006343 26.397815924285013 25.64985687706225 9 26.25907118406146 22.187279605269854 27.226720716476756 24.32692849419193 10 27.7050981904129 18.200879848005272 25.52319460443155 28.570827357150275 11 26.604846970117613 21.201608190012415 25.983516256009842 26.21002858386013 12 26.717661920338525 20.17792451715948 25.648431417402417 27.45598214509958 13 24.32156605070971 20.39741136525775 27.122594282277667 28.158428301754878 14 24.144639355312112 23.43547317454618 25.298718647523877 27.121168822617836 15 23.27130773705564 26.803087681381253 24.742483924548385 25.183120657014722 16 24.887745051788308 28.640640940966282 21.589468975549295 24.882145031696112 17 24.026020747904745 27.164882918852655 20.47784801749107 28.331248315751534 18 23.90787729371732 23.345737095008243 21.38552642564633 31.360859185628108 19 24.989326022309122 21.36285482915187 23.33643766770363 30.311381480835376 20 27.708152746826826 19.906815665665903 24.419006400313872 27.9660251871934 21 27.492670761582367 26.302411945623465 20.236707758369654 25.968209534424513 22 28.171189559661936 24.3503128205163 21.371271829048016 26.107225790773747 23 28.96890393691594 22.577414338359322 23.97887876058319 24.474802964141546 24 29.335586464649623 23.977928454143303 21.726618558534465 24.959866522672613 25 28.75455299302288 23.521592728763622 23.68651520082793 24.037339077385568 26 27.34665683492869 25.2961177874445 24.004648777278323 23.352576600348485 27 27.97506915849703 23.214617196443566 22.784011300792706 26.0263023442667 28 28.030027831392772 21.942709910603682 23.641296137682836 26.38596612032071 29 28.044088817650252 22.576952240300127 24.350091010975834 25.02886793107379 30 29.818878116046616 23.669772293710388 22.88441065725452 23.626938932988477 31 26.262271710294204 23.856341895783086 25.144425052290277 24.736961341632437 32 25.322324882800057 21.808310955350237 26.10532064994766 26.764043511902052 33 26.170464391891567 21.86045707987814 27.984755426201417 23.984323102028874 34 28.008870110049717 23.408445806808203 24.67108714082412 23.911596942317964 35 23.43044733271752 23.73127373442022 25.440601487987546 27.397677444874713 36 25.074602604570284 23.68083618424783 25.572230517700874 25.672330693481015 37 24.077987267851896 25.196265033127236 24.73984536809945 25.985902330921423 38 26.0251572617697 23.249593896078988 24.57596896896203 26.149279873189286 39 24.37451665958675 26.592397570733027 24.520588551075758 24.512497218604462 40 23.563220195789615 25.965835749035026 23.147002383771508 27.32394167140385 41 25.024111119259363 24.575741419394735 25.046547442318634 25.353600019027272 42 24.790324142351523 25.291977782881357 24.650606921683938 25.267091153083182 43 25.59899040741382 23.63252642533336 25.32980078430357 25.438682382949246 44 26.78765360362388 25.275003171303588 23.398368926953626 24.538974298118905 45 26.27076430577544 22.485722658860585 27.07705322652551 24.16645980883846 46 25.783302321067662 24.97082315413729 23.971553102703343 25.274321422091706 47 24.675633469259996 24.764679477367437 23.89859848204719 26.66108857132538 48 26.42619365768475 23.55006717106411 24.563657846605388 25.460081324645756 49 22.081368956113813 23.361878523923608 25.035049699424043 29.521702820538543 50 21.47052955910713 34.46432045948815 24.877776053712036 19.187373927692683 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15132.0 1 10986.0 2 6038.5 3 4068.5 4 2679.5 5 2307.5 6 1894.0 7 1794.5 8 2146.5 9 2304.0 10 2278.5 11 2619.5 12 3023.0 13 3105.5 14 3374.0 15 4327.5 16 4933.5 17 4521.0 18 4267.5 19 6707.0 20 8955.0 21 8167.0 22 7466.0 23 9336.0 24 11788.5 25 14640.0 26 16809.5 27 20702.0 28 26593.5 29 34812.0 30 41860.0 31 42999.5 32 50653.5 33 66415.0 34 81029.5 35 92269.5 36 93650.5 37 89172.5 38 88129.5 39 92733.5 40 102790.5 41 112977.0 42 125946.0 43 131945.5 44 133141.0 45 137374.0 46 148754.0 47 161785.5 48 168742.0 49 180623.0 50 211404.5 51 228268.5 52 217364.5 53 219051.5 54 218386.5 55 209177.0 56 209098.5 57 207129.5 58 206684.0 59 210790.0 60 204799.0 61 189280.0 62 166595.0 63 147352.0 64 136905.5 65 127173.0 66 113976.5 67 102864.0 68 94858.0 69 86795.5 70 77733.0 71 70758.5 72 69457.0 73 65630.0 74 55711.0 75 46507.0 76 41830.0 77 36311.5 78 30716.0 79 25399.5 80 19912.5 81 15588.5 82 12176.5 83 9953.5 84 8316.5 85 6153.0 86 4639.0 87 3932.0 88 2826.0 89 1741.0 90 1045.0 91 643.5 92 322.0 93 127.5 94 64.5 95 13.0 96 2.5 97 2.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 24439.0 25 26594.0 26 24082.0 27 24877.0 28 21492.0 29 25714.0 30 27194.0 31 25651.0 32 26194.0 33 22582.0 34 27953.0 35 24296.0 36 23640.0 37 25084.0 38 25974.0 39 23428.0 40 24529.0 41 23361.0 42 27842.0 43 27702.0 44 34728.0 45 39032.0 46 44266.0 47 101592.0 48 509464.0 49 902674.0 50 812034.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.73616489761743 #Duplication Level Percentage of deduplicated Percentage of total 1 67.36910406089498 5.885476020807266 2 13.071821967060835 2.2839518443308258 3 5.5890757748966395 1.4648126278432787 4 2.980999428373959 1.0417001026391284 5 1.7997449294256778 0.7861434238556783 6 1.1853513298263896 0.6213254807384068 7 0.8580433628784881 0.5247205815188863 8 0.6792632121363724 0.4747324344086912 9 0.528343480360969 0.41541261903130694 >10 3.750290623064373 6.652128114703229 >50 0.5878860462654125 3.614355362611478 >100 0.8796919807730177 19.06439428935331 >500 0.615862097846833 38.540540689502286 >1k 0.0975276886811073 13.322438122688308 >5k 0.005828347929149839 3.1644151090351653 >10k+ 0.0011656695858299677 2.1434531769327574 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 34297 1.1640235703148705 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 18791 0.6377574397115413 No Hit ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT 10057 0.34132970949810926 No Hit CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG 8557 0.2904204359327156 No Hit CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT 8330 0.282716165866486 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 7891 0.2678167184696808 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 6934 0.23533660193495964 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 6602 0.22406868271915253 No Hit ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT 6337 0.215074711055933 No Hit ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC 6253 0.21222379173627096 No Hit CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA 5717 0.19403221131557027 No Hit CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC 5508 0.18693885253212544 No Hit CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG 5403 0.1833752033825479 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 5343 0.18133883243993215 No Hit CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG 5211 0.1768588163661775 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 5050 0.17139455433682527 No Hit ACTTGTGTTTGGGAACAGATCAGGACTCAGGATGCAAGACCCTGGTCAT 5048 0.1713266753054047 No Hit AGCCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGA 5038 0.1709872801483021 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT 4989 0.16932424387849926 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 4915 0.16681271971593983 No Hit CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT 4786 0.16243452218931598 No Hit CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG 4697 0.1594139052911026 No Hit ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT 4678 0.15876905449260764 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG 4640 0.15747935289561768 No Hit ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT 4548 0.15435691745027352 No Hit ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTC 4135 0.14033989746193512 No Hit CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG 4128 0.14010232085196328 No Hit TCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGC 4052 0.13752291765798336 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 3970 0.13473987736974183 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 3786 0.12849500647905354 No Hit CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT 3686 0.1251010549080273 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAG 3639 0.12350589766964497 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3623 0.12296286541828078 No Hit ATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT 3458 0.11736284532608747 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 3299 0.11196646232815574 No Hit CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA 3172 0.10765614383295241 No Hit TCCCAGATACCCGGTGTCAGCTGGTGAAAGAAGGACGCGATGAAGTTG 3168 0.10752038577011137 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC 3057 0.10375309952627224 No Hit CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA 3049 0.10348158340059013 No Hit CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT 3049 0.10348158340059013 No Hit CTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGCC 3031 0.10287067211780543 No Hit AATTCGTGGAGAAAGAAATGGCTCC 2994 0.10161491003652572 No Hit AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT 2983 0.10124157536371282 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03244617701901088 0.0 0.0 0.0 0.0 2 0.05616989850048432 0.0 0.0 0.0 0.0 3 0.07239298700998975 0.0 0.0 0.0 0.0 4 0.08831061987810283 0.0 0.0 0.0 0.0 5 0.1109143373411376 0.0 0.0 0.0 0.0 6 0.1459738570698387 0.0 0.0 0.0 0.0 7 0.1817461066284553 0.0 0.0 0.0 0.0 8 0.2227450416064523 0.0 0.0 0.0 0.0 9 0.26516943624428035 0.0 0.0 0.0 0.0 10 0.3055574599394926 0.0 0.0 0.0 0.0 11 0.35045943922416983 0.0 0.0 0.0 0.0 12 0.39407171691185705 0.0 0.0 0.0 0.0 13 0.4387700591022727 0.0 0.0 0.0 0.0 14 0.4871678085051069 0.0 0.0 0.0 0.0 15 0.5380770820705005 0.0 0.0 0.0 0.0 16 0.5868142266304374 0.0 0.0 0.0 0.0 17 0.6408119961254649 0.0 0.0 0.0 0.0 18 0.6936897616020538 0.0 0.0 0.0 0.0 19 0.752439063296518 0.0 0.0 0.0 0.0 20 0.8087447198598434 0.0 0.0 0.0 0.0 21 0.8589073240796112 0.0 0.0 0.0 0.0 22 0.9075426500924173 0.0 0.0 0.0 0.0 23 0.9515961414843379 0.0 0.0 0.0 0.0 24 0.9977199433345846 0.0 0.0 0.0 0.0 25 1.0432328339020465 0.0 0.0 0.0 0.0 26 1.093599075216076 0.0 0.0 0.0 0.0 27 1.1359895303381937 0.0 0.0 0.0 0.0 28 1.1787872596488347 0.0 0.0 0.0 0.0 29 1.2375705008590092 0.0 0.0 0.0 0.0 30 1.2854252180104793 0.0 0.0 0.0 0.0 31 1.3329066004891363 0.0 0.0 0.0 0.0 32 1.3810667732819988 0.0 0.0 0.0 0.0 33 1.4302111920304588 0.0 0.0 0.0 0.0 34 1.4860077558581302 0.0 0.0 0.0 0.0 35 1.538240670536224 0.0 0.0 0.0 0.0 36 1.5910844964971025 0.0 0.0 0.0 0.0 37 1.6412131612011602 0.0 0.0 0.0 0.0 38 1.685436350171632 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGATA 15 1.2125449E-4 145.51889 44 ATGACGT 50 0.0 145.51889 44 CTATACA 75 0.0 135.81763 44 TCGTAAA 135 0.0 134.73972 44 CAATCCG 60 0.0 133.39232 44 GCGATCC 55 0.0 132.2899 44 CGTCACA 90 0.0 113.181366 44 GAACACA 3075 0.0 107.4237 44 CCGATAA 125 0.0 98.95285 44 GGGCAAT 60 7.2759576E-12 97.012596 44 AACGTAT 140 0.0 93.54786 44 CGGCAAA 100 0.0 87.31133 44 ATCACCG 220 0.0 85.98844 44 GATCAGT 120 0.0 84.886024 44 AATCGCA 265 0.0 82.36919 44 GGCATAA 160 0.0 81.85438 44 CTATCAT 1415 0.0 81.24377 44 CCGCCTA 215 0.0 77.83569 44 CTAACCT 165 0.0 70.55461 44 CCCGTAT 125 0.0 69.849075 44 >>END_MODULE