FastQCFastQC Report
Tue 24 May 2016
ERR840903.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840903.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9467011
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA489900.5174811775332256No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT336280.35521243188584023No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG256220.2706450853389734No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC237810.25119860957170115No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC210170.2220024884306145No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA138760.14657213348542641No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA134840.14243143902547487No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA133160.14065685568549566No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA113290.11966818249181288No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA112150.11846400093968412No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT110340.11655209865077795No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC105560.11150298652869421No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT101670.10739398105695662No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA95150.10050690761846585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG1500.058.2718944
CTATCAT49450.055.03664844
TAAGCCA80300.050.81390844
GTCGTAC1050.048.8719942
CGGTTAA900.046.407340
TAACGGT302.6072157E-643.80728527
GCGAATA1150.042.7279540
TACGCTC1850.041.82155643
GACGCTA39550.041.8132931
CGATATA1650.041.152327
CGGTTAT800.041.06933627
CACGATA200.001105886941.05031
CGGTCTA1300.041.05039
GGAGACA71550.040.8136544
TACGATA650.040.75633628
CCTATCA50600.039.4721243
AATCGCA15900.039.2310844
ACCGATA1000.038.9977872
CCGCTAG950.038.8897555
ACGCTAC42750.038.59290332
Produced by FastQC (version 0.11.5)