##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840903.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9467011
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.152293157787604	39.0	37.0	39.0	33.0	39.0
2	37.54366473219478	39.0	37.0	39.0	35.0	39.0
3	37.58643028934898	39.0	37.0	39.0	35.0	39.0
4	37.81448590267826	39.0	37.0	39.0	34.0	41.0
5	38.564686361936204	40.0	38.0	41.0	34.0	41.0
6	38.54552181253407	40.0	38.0	41.0	34.0	41.0
7	38.5324867584922	40.0	38.0	41.0	34.0	41.0
8	38.4331962855013	40.0	38.0	41.0	34.0	41.0
9	38.30707379552004	40.0	38.0	41.0	34.0	41.0
10	38.28528465848408	40.0	38.0	41.0	34.0	41.0
11	38.233914062210346	40.0	38.0	41.0	34.0	41.0
12	38.40175869659389	40.0	38.0	41.0	34.0	41.0
13	38.55678724784412	40.0	38.0	41.0	34.0	41.0
14	38.55813677622219	40.0	38.0	41.0	34.0	41.0
15	38.550852534131415	40.0	38.0	41.0	34.0	41.0
16	38.54567877865569	40.0	38.0	41.0	34.0	41.0
17	38.5496641970734	40.0	38.0	41.0	34.0	41.0
18	38.54629597451614	40.0	38.0	41.0	34.0	41.0
19	38.53120557269871	40.0	38.0	41.0	34.0	41.0
20	38.50732696940988	40.0	38.0	41.0	34.0	41.0
21	38.43820916654686	40.0	38.0	41.0	34.0	41.0
22	38.30148681563801	40.0	38.0	41.0	33.0	41.0
23	38.295027226650525	40.0	38.0	41.0	34.0	41.0
24	38.145814449777234	40.0	37.0	41.0	33.0	41.0
25	38.043218675189586	40.0	37.0	41.0	33.0	41.0
26	37.990553122325146	40.0	37.0	41.0	33.0	41.0
27	37.936808576722235	40.0	37.0	41.0	33.0	41.0
28	37.89985045285648	40.0	37.0	41.0	33.0	41.0
29	37.86897992880245	40.0	36.0	41.0	33.0	41.0
30	37.84679229595717	40.0	36.0	41.0	33.0	41.0
31	37.63044950717389	40.0	36.0	41.0	32.0	41.0
32	37.58956112690306	39.0	36.0	41.0	32.0	41.0
33	37.58982033398935	39.0	36.0	41.0	32.0	41.0
34	37.50945598060382	39.0	35.0	41.0	32.0	41.0
35	37.45618665369056	39.0	35.0	41.0	32.0	41.0
36	37.35667164283731	39.0	35.0	41.0	32.0	41.0
37	37.36610497862128	39.0	35.0	41.0	32.0	41.0
38	37.26922317371856	39.0	35.0	41.0	32.0	41.0
39	37.1715623893345	39.0	35.0	41.0	31.0	41.0
40	37.13311779132943	39.0	35.0	41.0	31.0	41.0
41	37.07288654424956	39.0	35.0	41.0	31.0	41.0
42	36.93482381861987	38.0	35.0	40.0	31.0	41.0
43	36.8593141796829	38.0	35.0	40.0	31.0	41.0
44	36.79144312065483	38.0	35.0	40.0	31.0	41.0
45	36.68488180794442	38.0	35.0	40.0	31.0	41.0
46	36.56832419422389	38.0	35.0	40.0	31.0	41.0
47	36.48946040308499	38.0	34.0	40.0	31.0	41.0
48	36.364432260731455	38.0	34.0	40.0	30.0	41.0
49	36.59605300771658	38.0	35.0	40.0	31.0	41.0
50	36.72781271409895	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	10.0
14	475.0
15	7935.0
16	14066.0
17	527.0
18	2.0
19	5.0
20	9.0
21	32.0
22	110.0
23	294.0
24	692.0
25	1626.0
26	3817.0
27	8750.0
28	19473.0
29	42079.0
30	83939.0
31	151902.0
32	253022.0
33	394284.0
34	565872.0
35	666250.0
36	888945.0
37	1145980.0
38	1357917.0
39	1861913.0
40	1997085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.088306119006305	13.212385619917416	31.699308261076283
2	25.75527798583946	22.64309189035483	31.36646825486946	20.235161868936245
3	24.493802743019945	18.568775297715405	29.493902563332817	27.44351939593183
4	21.65880022744243	19.78429094462867	33.2549735074777	25.301935320451197
5	20.08461804892801	23.181931445944237	30.651300605861763	26.08214989926599
6	20.704106079521825	26.019733155480644	28.942926125257486	24.333234639740038
7	27.466419971414417	25.022797586270894	24.167163215506985	23.343619226807704
8	25.249479481961096	24.931417107258035	25.179214432094778	24.63988897868609
9	23.694817720186446	23.867322009026925	28.380520525433	24.05733974535363
10	25.226673973443148	22.79898058637515	26.10854682644818	25.86579861373352
11	25.67568581044218	23.788870637205346	26.409740096425367	24.125703455927113
12	26.00099440045015	22.340261355986595	26.569378656050997	25.08936558751226
13	24.72511123098938	21.58538740474686	27.38571868142965	26.30378268283411
14	22.473492425433964	23.86391016129589	26.577216399135906	27.085381014134242
15	21.44129757533819	25.704227025826842	27.731730743737387	25.122744655097577
16	25.245993693257567	26.723439953750976	23.766529900514534	24.264036452476923
17	24.69209130527048	29.0016352574218	21.214837502565487	25.091435934742236
18	23.004335792997388	24.283916011083118	24.114327109158317	28.597421086761177
19	24.332960001842185	25.182573464845454	24.812678468420497	25.671788064891864
20	26.48713516863982	22.659031451426433	25.11939618534298	25.734437194590775
21	25.434849500016426	24.25672685919558	25.120579240902963	25.187844399885034
22	24.274345936642515	26.05608042496201	25.46741521690426	24.20215842149122
23	26.516785498611974	24.71990367392623	25.779615128787746	22.983695698674058
24	26.35536179265029	24.491901403727113	24.98860516798808	24.16413163563452
25	25.438715757297604	26.946685144060478	24.368733372952065	23.245865725689853
26	25.810529229310493	26.244675995575307	25.47132897154957	22.47346580356463
27	24.511460721657713	26.154192918013834	25.958874267460697	23.375472092867756
28	25.20047530049568	24.47708471933675	25.109797543194425	25.212642436973148
29	25.91930499746926	25.836463982754893	24.732434899028146	23.511796120747697
30	26.179072326990365	24.96202045561361	24.126288824650487	24.732618392745536
31	23.483018860804854	27.028019576091083	25.476531009405928	24.01243055369813
32	24.50306834525252	24.741974083811538	26.55494837580006	24.200009195135884
33	24.04228644042158	24.240871598605455	25.987510907203365	25.7293310537696
34	24.666367006508526	25.647776725940354	26.63729089055821	23.04856537699291
35	23.6405000925394	24.47487606697702	26.38353269912386	25.501091141359716
36	24.171564436106248	25.389630933241836	27.49892313638028	22.93988149427164
37	23.626408144083776	26.398505922417907	25.981115474346755	23.993970459151555
38	23.72315790583349	26.105151081765825	26.305375271012473	23.866315741388217
39	24.558940969464913	26.569544547295664	24.578341934270288	24.29317254896913
40	22.98060252846405	27.187919304176823	25.734777678135973	24.096700489223156
41	24.521913387528596	25.551465870009572	26.22681171084015	23.699809031621687
42	23.569079327376407	26.893984160483996	26.061533172537406	23.475403339602188
43	24.115215076979645	25.243374412353393	26.407178862617886	24.234231648049075
44	24.79292271553154	27.18708091869099	25.431008800221754	22.588987565555712
45	24.03459514387552	26.606481796576087	26.006777124015336	23.352145935533056
46	23.494457839720024	27.767741489217485	24.35449175384816	24.383308917214336
47	25.39421613862323	25.74374201822363	25.015217113287342	23.846824729865805
48	22.87140051385205	26.738094343342045	25.80432614111878	24.586179001687128
49	22.543662063883524	26.32795293567634	25.594434972869358	25.533950027570775
50	21.10867632036021	30.088522132370414	26.285104061786253	22.517697485483122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19266.0
1	16348.5
2	11237.5
3	8841.5
4	8557.0
5	7597.0
6	6664.0
7	7073.5
8	8333.0
9	10014.0
10	11319.5
11	12896.0
12	14996.0
13	19939.5
14	25917.5
15	30274.5
16	33458.0
17	42113.5
18	49823.5
19	54323.5
20	58725.5
21	71741.0
22	86037.5
23	100688.5
24	115629.0
25	134396.5
26	151573.5
27	160816.5
28	176432.5
29	200551.0
30	218053.0
31	231814.0
32	256022.0
33	290472.0
34	317055.0
35	330792.0
36	348185.5
37	357473.5
38	365201.0
39	378955.5
40	404817.5
41	456859.0
42	497857.0
43	529554.0
44	566291.5
45	540374.5
46	518636.0
47	537406.5
48	542167.5
49	550141.5
50	596515.0
51	646336.0
52	654121.0
53	628250.5
54	605139.0
55	575612.0
56	539017.0
57	526970.5
58	517175.5
59	489756.5
60	463557.0
61	441826.5
62	407054.0
63	372711.0
64	342761.5
65	324882.5
66	311046.0
67	292374.5
68	274947.0
69	256415.0
70	221203.5
71	186842.0
72	164689.0
73	150094.5
74	145323.0
75	130955.5
76	103026.5
77	69897.5
78	47307.0
79	39275.0
80	32818.0
81	26449.0
82	21228.0
83	16330.0
84	12613.0
85	9691.0
86	6983.0
87	5250.5
88	3632.5
89	2312.0
90	1390.5
91	824.0
92	567.5
93	411.5
94	338.0
95	237.0
96	174.5
97	177.0
98	93.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	67417.0
25	63811.0
26	63243.0
27	67416.0
28	63517.0
29	58428.0
30	72800.0
31	70870.0
32	61202.0
33	68327.0
34	67758.0
35	68886.0
36	61735.0
37	74171.0
38	68779.0
39	64102.0
40	67043.0
41	70713.0
42	80538.0
43	79982.0
44	83991.0
45	99920.0
46	118648.0
47	219819.0
48	790469.0
49	1894990.0
50	4898436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.131463555971933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.17406588360437	6.225289980785629
2	13.518925460298176	2.468951503132278
3	5.497955633916275	1.5061314451037109
4	2.8687100051789036	1.0478208345977287
5	1.6825279972454699	0.7681971544374726
6	1.1809012838853057	0.6470014222199482
7	0.8248310610336663	0.5272340341563809
8	0.5926175118494608	0.43291721696672936
9	0.4714605123925069	0.3874612038292828
>10	3.2351712837966895	5.9119983117729715
>50	0.472273532413649	3.028208252924207
>100	0.7348136783673874	17.064866014077843
>500	0.631514031654392	42.11772574336262
>1k	0.11087915715058667	14.03664470600979
>5k	0.0018499129451609872	1.2110698509839541
>10k+	0.0015030542679433023	2.618482325639535
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	48990	0.5174811775332256	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	33628	0.35521243188584023	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG	25622	0.2706450853389734	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23781	0.25119860957170115	No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC	21017	0.2220024884306145	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA	13876	0.14657213348542641	No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA	13484	0.14243143902547487	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	13316	0.14065685568549566	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	11329	0.11966818249181288	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	11215	0.11846400093968412	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	11034	0.11655209865077795	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	10556	0.11150298652869421	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT	10167	0.10739398105695662	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9515	0.10050690761846585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04442796147590829	0.0	0.0	0.0	0.0
2	0.08986997057466184	0.0	0.0	0.0	0.0
3	0.1184217489553989	0.0	0.0	0.0	0.0
4	0.15151561564679708	0.0	0.0	0.0	0.0
5	0.18675377054067013	0.0	0.0	0.0	0.0
6	0.2458748595517635	0.0	0.0	0.0	0.0
7	0.2962075358315312	0.0	0.0	0.0	0.0
8	0.35907848844793777	0.0	0.0	0.0	0.0
9	0.41283357545480825	0.0	0.0	0.0	0.0
10	0.474183456636947	0.0	0.0	0.0	0.0
11	0.5338221324555342	0.0	0.0	0.0	0.0
12	0.5915911579695006	0.0	0.0	0.0	0.0
13	0.6496559473734634	0.0	0.0	0.0	0.0
14	0.713667703565571	0.0	0.0	0.0	0.0
15	0.7880523218996999	0.0	0.0	0.0	0.0
16	0.8531203776989379	0.0	0.0	0.0	0.0
17	0.9378144801986604	0.0	0.0	0.0	0.0
18	0.9993122433257974	0.0	0.0	0.0	0.0
19	1.0744257083888462	0.0	0.0	0.0	0.0
20	1.1418810013001992	0.0	0.0	0.0	0.0
21	1.2057448755473084	0.0	0.0	0.0	0.0
22	1.2692918599122784	0.0	0.0	0.0	0.0
23	1.3238497346205682	0.0	0.0	0.0	0.0
24	1.37640064007531	0.0	0.0	0.0	0.0
25	1.4301662900782517	0.0	0.0	0.0	0.0
26	1.4914844822721764	0.0	0.0	0.0	0.0
27	1.5420072924812278	0.0	0.0	0.0	0.0
28	1.591526618063505	0.0	0.0	0.0	0.0
29	1.658781214049503	0.0	0.0	0.0	0.0
30	1.7100856859678308	0.0	0.0	0.0	0.0
31	1.7669251678275224	0.0	0.0	0.0	0.0
32	1.8195922662390485	0.0	0.0	0.0	0.0
33	1.8748684246801868	0.0	0.0	0.0	0.0
34	1.935647904074475	0.0	0.0	0.0	0.0
35	1.993300736631657	0.0	0.0	0.0	0.0
36	2.051988742803827	0.0	0.0	0.0	0.0
37	2.105458628916772	0.0	0.0	0.0	0.0
38	2.159118648959001	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	150	0.0	58.27189	44
CTATCAT	4945	0.0	55.036648	44
TAAGCCA	8030	0.0	50.813908	44
GTCGTAC	105	0.0	48.87199	42
CGGTTAA	90	0.0	46.4073	40
TAACGGT	30	2.6072157E-6	43.807285	27
GCGAATA	115	0.0	42.72795	40
TACGCTC	185	0.0	41.821556	43
GACGCTA	3955	0.0	41.81329	31
CGATATA	165	0.0	41.1523	27
CGGTTAT	80	0.0	41.069336	27
CACGATA	20	0.0011058869	41.0503	1
CGGTCTA	130	0.0	41.0503	9
GGAGACA	7155	0.0	40.81365	44
TACGATA	65	0.0	40.756336	28
CCTATCA	5060	0.0	39.47212	43
AATCGCA	1590	0.0	39.23108	44
ACCGATA	100	0.0	38.997787	2
CCGCTAG	95	0.0	38.889755	5
ACGCTAC	4275	0.0	38.592903	32
>>END_MODULE