FastQCFastQC Report
Tue 24 May 2016
ERR840902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363158
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC50830.3728841410900277No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT43410.3184517128608716No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA34920.2561698643884275No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG32170.23599612077250032No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA26010.19080693507282354No Hit
CCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAA25720.1886795221096894No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC22970.16850577849376228No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA21920.16080307638586283No Hit
TACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGAT20570.15089960224713495No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA20240.14847875301322372No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT19530.1432702592069298No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA18230.13373358040667332No Hit
AGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATG18070.13255983532356483No Hit
CCGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG17930.1315328083758449No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG16450.1206756663570914No Hit
TTCTGAAACCAACTTTTTACTTCCATCATCCTTTTTTTAGCCTGTTGCTT15570.11422006839999471No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT15460.11341311865535764No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGG15370.1127528870461091No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG14880.10915829272908936No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA14720.10798454764598088No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACAC14710.10791118857828659No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA14630.10732431603673236No Hit
AAGCAAGGAGCAGGCCAAAGCCACCCTTCTGGAGGACCAGCAAGACCCTT14040.1029961310427698No Hit
AGGTACATGCAGTTCAGCAACTCGCCAAGGTTATGGGCTGGCAAGTACTG13910.10204246316274415No Hit
AGATCCTCCCACTTCAGCCTCCTGAGTAGCTGGGACTACAGATACGCAC13880.10182238595966132No Hit
ATGGACCTTTCTGTGAGCACACACACCTGTAGTCCTAGCTACTCAACTAG13840.10152894968888419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTT1300.072.7859144
CGTATAA850.072.7859144
CATTGCG1350.072.7859144
GTAGCAA700.072.7859144
TGCCTTA150.001905582572.7859144
TGTACTC2050.072.7859144
CTACTGT453.6379788E-1272.78590444
TGTCGCA550.072.78590444
ATAAGCA1450.070.2760544
GACGGTT1150.069.6213144
TGCAACA1150.069.6213144
TTCGTCT900.068.7422444
AACACGA900.068.7422444
GACGTCC700.067.58691444
GTCAATC650.067.18744
CGGACAT1050.065.8539244
GGGCTTA509.094947E-1265.5073244
GTCCGAT950.065.1242444
AATGGTC1350.064.69858644
CGTGCGC850.064.2228644
Produced by FastQC (version 0.11.5)