##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840902.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1363158
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.001879459314324	38.0	35.0	39.0	31.0	39.0
2	36.726749943880314	39.0	35.0	39.0	33.0	39.0
3	36.82892298618356	39.0	35.0	39.0	33.0	39.0
4	36.91414274794265	39.0	35.0	39.0	33.0	40.0
5	37.628324816345575	39.0	35.0	40.0	33.0	41.0
6	37.61289887159082	39.0	35.0	40.0	33.0	41.0
7	37.673816241404154	39.0	36.0	40.0	33.0	41.0
8	37.5611411149698	39.0	35.0	40.0	33.0	41.0
9	37.35901487575175	39.0	35.0	40.0	32.0	41.0
10	37.425787766348435	39.0	35.0	40.0	33.0	41.0
11	37.15491821197543	38.0	35.0	40.0	32.0	41.0
12	37.36028838916692	39.0	35.0	40.0	33.0	41.0
13	37.54113683080025	39.0	35.0	41.0	33.0	41.0
14	37.58704273459129	39.0	35.0	41.0	33.0	41.0
15	37.66660577864048	39.0	35.0	41.0	33.0	41.0
16	37.65224427395797	39.0	35.0	41.0	33.0	41.0
17	37.669306859512986	39.0	35.0	41.0	33.0	41.0
18	37.64883161012883	39.0	35.0	41.0	33.0	41.0
19	37.63148219061914	39.0	35.0	41.0	33.0	41.0
20	37.62723103264625	39.0	35.0	41.0	33.0	41.0
21	37.60947006876679	39.0	35.0	41.0	33.0	41.0
22	37.38065286635885	39.0	35.0	41.0	32.0	41.0
23	37.40718317319049	39.0	35.0	41.0	32.0	41.0
24	37.23819689280333	39.0	35.0	41.0	32.0	41.0
25	37.16977712241739	39.0	35.0	41.0	32.0	41.0
26	37.02761731033942	39.0	35.0	41.0	31.0	41.0
27	36.98833288505086	39.0	35.0	41.0	31.0	41.0
28	36.95452044495845	39.0	35.0	41.0	31.0	41.0
29	36.948216047930536	38.0	35.0	40.0	31.0	41.0
30	36.890535981057496	38.0	35.0	40.0	31.0	41.0
31	36.68006622036962	38.0	35.0	40.0	31.0	41.0
32	36.63177987070625	38.0	35.0	40.0	31.0	41.0
33	36.631570616693615	38.0	35.0	40.0	31.0	41.0
34	36.62673477070718	38.0	35.0	40.0	31.0	41.0
35	36.53986734888466	38.0	35.0	40.0	31.0	41.0
36	36.43406983470705	38.0	34.0	40.0	31.0	41.0
37	36.43798880582078	37.0	34.0	40.0	31.0	41.0
38	36.31093015414774	37.0	34.0	40.0	31.0	41.0
39	36.138837526194976	37.0	34.0	40.0	31.0	41.0
40	36.153251024799175	37.0	34.0	40.0	31.0	41.0
41	36.03991654098984	37.0	34.0	40.0	31.0	41.0
42	35.910202708012314	36.0	34.0	40.0	30.0	41.0
43	35.77372310616612	36.0	34.0	39.0	30.0	41.0
44	35.68962056126673	36.0	34.0	39.0	30.0	41.0
45	35.53085900221503	36.0	34.0	39.0	30.0	41.0
46	35.36433482142857	35.0	34.0	39.0	30.0	41.0
47	35.25952168298671	35.0	34.0	39.0	30.0	41.0
48	35.15404173496584	35.0	34.0	39.0	30.0	40.0
49	35.29587115320246	35.0	34.0	39.0	30.0	40.0
50	35.24211624894455	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	11.0
14	394.0
15	1789.0
16	274.0
17	3.0
18	0.0
19	1.0
20	1.0
21	15.0
22	63.0
23	178.0
24	503.0
25	1157.0
26	2546.0
27	5100.0
28	9590.0
29	17157.0
30	28513.0
31	44463.0
32	63876.0
33	91433.0
34	125211.0
35	101544.0
36	133614.0
37	165623.0
38	189052.0
39	231497.0
40	149550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.65857516149999	13.702813613682347	34.638611224817666
2	27.571198643150684	23.227681604040033	28.093075050727794	21.10804470208149
3	29.44919077612426	15.085705398787228	25.571503816872294	29.893600008216215
4	21.51951571277871	18.58331902831513	32.574800573374475	27.322364685531685
5	20.503345907077534	27.3324148778058	28.01142640838406	24.1528128067326
6	20.458523516716333	30.89333738275387	28.406538346985457	20.241600753544343
7	29.200356818505263	28.849040243317354	22.40569325052562	19.544909687651764
8	26.269735423186454	25.842638931070354	24.94831853680938	22.939307108933814
9	23.200465389925455	26.357179431878038	26.850885957460548	23.591469220735966
10	26.99731065657833	23.13165458442822	25.576712310678584	24.29432244831487
11	26.93187436819503	24.32359271632489	26.82264271639825	21.921890199081837
12	27.432109850802327	21.888218387010163	27.16427589465051	23.515395867537
13	25.179766395384835	19.847002328416806	29.947225486700734	25.026005789497624
14	20.2228941912823	23.544079263005464	28.92900162710412	27.304024918608118
15	20.491975251584922	25.536438182514427	29.474939808884958	24.496646757015693
16	22.554392080741927	31.550928065565397	21.993121853812983	23.901557999879692
17	23.493608224431796	32.013603705513226	22.368573562272314	22.124214507782664
18	22.237480908302633	27.694001722470908	22.37796352293718	27.69055384628928
19	22.797944185486934	25.000623552075403	26.056553972466872	26.14487828997079
20	24.504716254462064	22.36409865914296	26.641152382922595	26.490032703472377
21	24.442727842260396	24.658110065010806	26.455407223520677	24.443754869208117
22	23.77339970861778	24.811283798356463	27.87651908289428	23.538797410131473
23	26.34955008883783	24.243998127876594	26.138349332946	23.26810245033958
24	26.087218062763085	23.664828288430247	27.116225705310754	23.13172794349591
25	25.914630764486603	24.282334775602745	25.476625548061143	24.32640891184951
26	24.537305415356382	28.335925930070765	24.438674828683446	22.68809382588941
27	24.375096731344563	25.454656102226437	26.731284457019726	23.438962709409275
28	26.191100527929645	27.827182238039732	21.92044829305779	24.061268940972827
29	25.207679640215456	26.790062540672828	25.376481407363727	22.625776411747992
30	26.77182072320595	26.572118276774052	23.560042279069553	23.096018720950447
31	22.53127830075915	27.49788389164591	26.009405099061905	23.96143270853304
32	25.06664990927443	24.62755386585184	27.25372524684424	23.052070978029487
33	20.886526253975866	27.783700193342465	26.585815801532338	24.74395775114933
34	24.578470658048378	28.428146196106457	26.081938604271283	20.91144454157388
35	24.1696833544012	23.98968787641544	27.477512634684814	24.363116134498544
36	23.02223296788175	25.73354676809415	29.086138450791417	22.158081813232684
37	24.357592834810287	25.84138120910534	27.882019135167553	21.919006820916824
38	23.778932069968683	26.11798126551349	27.373734292782615	22.72935237173521
39	26.423107548483603	25.98533081461788	27.074108871142382	20.51745276575613
40	25.567721533332943	25.75089677797935	25.889327565523768	22.792054123163947
41	23.42932192094596	26.70849214057627	25.288266371082674	24.573919567395098
42	23.741146685708625	27.462784476249375	25.548538199510055	23.247530638531945
43	24.66552768274267	27.45911430057274	25.891904618576937	21.983453398107656
44	23.86571871547797	27.632597240588026	26.192998660251426	22.308685383682576
45	24.701463892185416	26.997401124770658	26.667787681049692	21.63334730199423
46	22.178839285714286	26.825267857142858	26.61625	24.37964285714286
47	25.467408330414997	26.90286611955844	25.530118552181147	22.099606997845413
48	21.96969342371447	28.621057331984044	25.161460639551702	24.247788604749783
49	22.309327237248844	28.30658524276392	25.995475605471967	23.388611914515263
50	25.272581381078997	26.584354694063784	26.548036732274998	21.59502719258222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2695.0
1	2384.0
2	1747.0
3	1467.5
4	1490.0
5	1534.5
6	1594.5
7	1336.0
8	1193.5
9	1753.5
10	2314.0
11	2435.0
12	2682.0
13	3551.5
14	4089.0
15	3775.5
16	3667.5
17	5162.5
18	6443.5
19	6044.5
20	5719.0
21	8942.0
22	12250.0
23	12172.5
24	12354.0
25	15448.5
26	19140.0
27	25127.5
28	30364.5
29	34328.0
30	39296.5
31	42039.0
32	43763.5
33	49168.5
34	54691.5
35	55720.0
36	59111.0
37	60680.5
38	60261.5
39	67112.0
40	73858.0
41	72231.5
42	70771.5
43	73703.0
44	77969.5
45	78266.0
46	74997.5
47	76589.5
48	81047.5
49	77049.0
50	72840.5
51	75948.5
52	79623.5
53	74668.0
54	73526.5
55	81414.5
56	77966.0
57	72742.5
58	74687.0
59	73566.0
60	68951.5
61	63035.0
62	56662.5
63	53436.5
64	50499.5
65	44262.0
66	39461.5
67	39242.0
68	40722.5
69	37986.5
70	32078.5
71	27036.0
72	23915.0
73	22165.5
74	21465.5
75	19193.0
76	15072.0
77	11266.5
78	8077.5
79	6966.5
80	5522.5
81	3670.0
82	3145.0
83	3282.5
84	2746.0
85	2220.5
86	1551.0
87	987.5
88	761.0
89	644.0
90	414.5
91	116.5
92	23.0
93	8.0
94	5.5
95	4.0
96	4.0
97	4.0
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10891.0
25	11912.0
26	9349.0
27	11512.0
28	10266.0
29	9294.0
30	15737.0
31	11127.0
32	9509.0
33	9793.0
34	10959.0
35	10270.0
36	8510.0
37	10972.0
38	12445.0
39	9403.0
40	10270.0
41	10191.0
42	11235.0
43	10790.0
44	12847.0
45	15876.0
46	14917.0
47	36866.0
48	103226.0
49	202283.0
50	762708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.83802837416375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.96703447040728	6.784996420017962
2	13.443031800013408	2.64505856566635
3	5.31426848149055	1.568457723264844
4	2.7170822842431437	1.0692293042928682
5	1.664855465887034	0.8189447656139125
6	1.061661939710917	0.6266796171987437
7	0.7241762905097934	0.4987126825752398
8	0.5576589586958861	0.4389011727005392
9	0.4532278254265489	0.401298138585623
>10	3.1410935252176606	6.320633535285519
>50	0.4979219452018718	3.501817723879234
>100	0.7431559571351289	17.9814193202842
>500	0.604513020297482	43.44298470692187
>1k	0.10957264362975322	13.528120328896472
>5k	7.453921335357362E-4	0.3727459948166219
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	5083	0.3728841410900277	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	4341	0.3184517128608716	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	3492	0.2561698643884275	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG	3217	0.23599612077250032	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	2601	0.19080693507282354	No Hit
CCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAA	2572	0.1886795221096894	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	2297	0.16850577849376228	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGA	2192	0.16080307638586283	No Hit
TACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGAT	2057	0.15089960224713495	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	2024	0.14847875301322372	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	1953	0.1432702592069298	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1823	0.13373358040667332	No Hit
AGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATG	1807	0.13255983532356483	No Hit
CCGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	1793	0.1315328083758449	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	1645	0.1206756663570914	No Hit
TTCTGAAACCAACTTTTTACTTCCATCATCCTTTTTTTAGCCTGTTGCTT	1557	0.11422006839999471	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	1546	0.11341311865535764	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGG	1537	0.1127528870461091	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	1488	0.10915829272908936	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1472	0.10798454764598088	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACAC	1471	0.10791118857828659	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1463	0.10732431603673236	No Hit
AAGCAAGGAGCAGGCCAAAGCCACCCTTCTGGAGGACCAGCAAGACCCTT	1404	0.1029961310427698	No Hit
AGGTACATGCAGTTCAGCAACTCGCCAAGGTTATGGGCTGGCAAGTACTG	1391	0.10204246316274415	No Hit
AGATCCTCCCACTTCAGCCTCCTGAGTAGCTGGGACTACAGATACGCAC	1388	0.10182238595966132	No Hit
ATGGACCTTTCTGTGAGCACACACACCTGTAGTCCTAGCTACTCAACTAG	1384	0.10152894968888419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0608880261862528	0.0	0.0	0.0	0.0
2	0.08715057242080522	0.0	0.0	0.0	0.0
3	0.1086447792552294	0.0	0.0	0.0	0.0
4	0.13820848353602444	0.0	0.0	0.0	0.0
5	0.17591504433088462	0.0	0.0	0.0	0.0
6	0.2379768156002459	0.0	0.0	0.0	0.0
7	0.2880810588354395	0.0	0.0	0.0	0.0
8	0.3447876181631183	0.0	0.0	0.0	0.0
9	0.3982663785122488	0.0	0.0	0.0	0.0
10	0.45548645131378757	0.0	0.0	0.0	0.0
11	0.5127065241153264	0.0	0.0	0.0	0.0
12	0.5711003419999736	0.0	0.0	0.0	0.0
13	0.6313281365769778	0.0	0.0	0.0	0.0
14	0.6961775524187218	0.0	0.0	0.0	0.0
15	0.7594130687711916	0.0	0.0	0.0	0.0
16	0.8293976193515352	0.0	0.0	0.0	0.0
17	0.9016563010304015	0.0	0.0	0.0	0.0
18	0.9608570686596858	0.0	0.0	0.0	0.0
19	1.0254130482306527	0.0	0.0	0.0	0.0
20	1.0934169039832506	0.0	0.0	0.0	0.0
21	1.1644284815113142	0.0	0.0	0.0	0.0
22	1.2171002921158076	0.0	0.0	0.0	0.0
23	1.2646369679817013	0.0	0.0	0.0	0.0
24	1.3169419832477234	0.0	0.0	0.0	0.0
25	1.3689535622429683	0.0	0.0	0.0	0.0
26	1.4258068397060355	0.0	0.0	0.0	0.0
27	1.4742238243842607	0.0	0.0	0.0	0.0
28	1.5230076044009573	0.0	0.0	0.0	0.0
29	1.5758261331408392	0.0	0.0	0.0	0.0
30	1.6279110712037783	0.0	0.0	0.0	0.0
31	1.6808029590113545	0.0	0.0	0.0	0.0
32	1.7305404069080768	0.0	0.0	0.0	0.0
33	1.7837990900541243	0.0	0.0	0.0	0.0
34	1.840945803787969	0.0	0.0	0.0	0.0
35	1.899633057943393	0.0	0.0	0.0	0.0
36	1.9561928991356834	0.0	0.0	0.0	0.0
37	2.015246948629579	0.0	0.0	0.0	0.0
38	2.0682855545725443	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTT	130	0.0	72.78591	44
CGTATAA	85	0.0	72.78591	44
CATTGCG	135	0.0	72.78591	44
GTAGCAA	70	0.0	72.78591	44
TGCCTTA	15	0.0019055825	72.78591	44
TGTACTC	205	0.0	72.78591	44
CTACTGT	45	3.6379788E-12	72.785904	44
TGTCGCA	55	0.0	72.785904	44
ATAAGCA	145	0.0	70.27605	44
GACGGTT	115	0.0	69.62131	44
TGCAACA	115	0.0	69.62131	44
TTCGTCT	90	0.0	68.74224	44
AACACGA	90	0.0	68.74224	44
GACGTCC	70	0.0	67.586914	44
GTCAATC	65	0.0	67.187	44
CGGACAT	105	0.0	65.85392	44
GGGCTTA	50	9.094947E-12	65.50732	44
GTCCGAT	95	0.0	65.12424	44
AATGGTC	135	0.0	64.698586	44
CGTGCGC	85	0.0	64.22286	44
>>END_MODULE