FastQCFastQC Report
Tue 24 May 2016
ERR840901.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840901.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673600
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA104020.6215344168260039No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC62010.3705186424474187No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT49970.2985779158699809No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35910.2145673996175908No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC31560.1885755258126195No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG30710.18349665391969408No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC27150.16222514340344169No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC24780.1480640535372849No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG22530.1346199808795411No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCG21700.12966061185468453No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC20110.12016013384321224No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA20080.11998087954110898No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC19470.11633604206500957No Hit
AACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAAT19330.11549952198852773No Hit
AGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGAGCAGACGCTGTGTAT18990.11346797323135754No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT18570.11095841300191205No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT17280.10325047801147226No Hit
CATTATAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATA16990.1015176864244742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACAT2250.071.5330944
AATATAC500.071.5330944
ACTACCC950.071.5330944
TTAGTCC800.071.5330944
CGGTCTT1300.071.5330944
ATTATGC950.071.5330944
TATACAA207.12129E-571.5330944
CGATATA700.071.5330944
GTAGTTA252.4976453E-671.5330944
CGGATTA1550.071.5330844
TGTCCGA150.002041607471.5330844
GCCACTC1100.068.28158644
TTCGTAG850.067.3252644
CCCGAAC1350.066.2343444
TAACTCC650.066.0305444
ATCCTCG501.0913936E-1164.37977644
GTTCGGG1000.064.37977644
ACGCTTA501.0913936E-1164.37977644
GAATTTC850.063.11742844
GCCAATA1900.062.1208444
Produced by FastQC (version 0.11.5)