##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840901.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673600
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.54762249043977	39.0	37.0	39.0	31.0	39.0
2	37.10016252390057	39.0	37.0	39.0	33.0	39.0
3	37.17171546367113	39.0	37.0	39.0	33.0	39.0
4	37.31802461759082	39.0	37.0	39.0	33.0	40.0
5	38.01985898661568	39.0	37.0	40.0	33.0	41.0
6	38.054553059273424	39.0	37.0	40.0	33.0	41.0
7	38.057032743785854	39.0	37.0	40.0	33.0	41.0
8	37.94961400573614	39.0	37.0	40.0	33.0	41.0
9	37.78899318833652	39.0	37.0	40.0	33.0	41.0
10	37.780602294455065	39.0	37.0	40.0	33.0	41.0
11	37.70748207456979	39.0	37.0	40.0	33.0	41.0
12	37.882483269598474	39.0	37.0	40.0	33.0	41.0
13	38.07292901529637	39.0	37.0	41.0	34.0	41.0
14	38.10364722753346	40.0	37.0	41.0	34.0	41.0
15	38.117507767686426	40.0	37.0	41.0	34.0	41.0
16	38.113818116634796	40.0	37.0	41.0	33.0	41.0
17	38.12267566921606	40.0	37.0	41.0	33.0	41.0
18	38.1059464627151	40.0	37.0	41.0	33.0	41.0
19	38.02827318355641	40.0	37.0	41.0	33.0	41.0
20	38.08993726099426	40.0	37.0	41.0	33.0	41.0
21	37.982343451242826	40.0	37.0	41.0	33.0	41.0
22	37.885436782982794	40.0	36.0	41.0	33.0	41.0
23	37.84592495219885	40.0	36.0	41.0	33.0	41.0
24	37.72758544455067	39.0	36.0	41.0	33.0	41.0
25	37.634662029418045	39.0	36.0	41.0	33.0	41.0
26	37.51291516491504	39.0	35.0	41.0	32.0	41.0
27	37.48396752078454	39.0	35.0	41.0	32.0	41.0
28	37.46640117811059	39.0	35.0	41.0	32.0	41.0
29	37.412383300407356	39.0	35.0	41.0	32.0	41.0
30	37.39619648384733	39.0	35.0	41.0	32.0	41.0
31	37.32968669583725	39.0	35.0	41.0	32.0	41.0
32	37.10228418147519	39.0	35.0	41.0	31.0	41.0
33	37.1038566014828	39.0	35.0	40.0	31.0	41.0
34	37.02809646981816	38.0	35.0	40.0	31.0	41.0
35	36.99111654007276	38.0	35.0	40.0	31.0	41.0
36	36.979750056566836	38.0	35.0	40.0	31.0	41.0
37	36.911085459502246	38.0	35.0	40.0	31.0	41.0
38	36.81035069549667	38.0	35.0	40.0	31.0	41.0
39	36.662221920817764	38.0	35.0	40.0	31.0	41.0
40	36.62338707221762	38.0	35.0	40.0	31.0	41.0
41	36.47765658275542	38.0	34.0	40.0	31.0	41.0
42	36.32825244034987	37.0	34.0	40.0	31.0	41.0
43	36.33775371541933	37.0	34.0	40.0	31.0	41.0
44	36.22215978330187	37.0	34.0	40.0	31.0	41.0
45	36.112294234807244	37.0	34.0	40.0	31.0	41.0
46	35.86565914622568	36.0	34.0	40.0	30.0	41.0
47	35.753334896423375	36.0	34.0	39.0	30.0	41.0
48	35.651184008688695	36.0	34.0	39.0	30.0	41.0
49	35.696849665521455	36.0	34.0	39.0	30.0	41.0
50	35.53464261570368	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	23.0
14	319.0
15	2190.0
16	325.0
17	3.0
18	0.0
19	1.0
20	12.0
21	39.0
22	75.0
23	176.0
24	347.0
25	680.0
26	1333.0
27	2867.0
28	5953.0
29	11415.0
30	21207.0
31	36640.0
32	58343.0
33	88744.0
34	122008.0
35	138095.0
36	180192.0
37	217051.0
38	252422.0
39	318157.0
40	214983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	57.2828035372849	11.998924474187382	30.718271988527725
2	26.219228011472275	24.60701481835564	31.75878346080306	17.414973709369026
3	25.295470841300194	19.24880497131931	24.603728489483746	30.85199569789675
4	21.58622131931166	22.25406309751434	31.790093212237093	24.369622370936902
5	18.36376673040153	25.80437380497132	30.10593929254302	25.725920172084134
6	20.45889101338432	31.302402007648183	26.39107313575526	21.847633843212236
7	28.88330544933078	27.785492351816444	22.6598948374761	20.671307361376673
8	26.675549713193114	25.922024378585085	24.739842256214146	22.662583652007648
9	22.02270554493308	26.756154397705544	28.01894120458891	23.202198852772465
10	25.24892447418738	24.872191682600384	25.988826481835563	23.890057361376673
11	24.762906309751433	25.1875	27.19502868068834	22.85456500956023
12	24.672263384321226	23.74689292543021	27.49193355640535	24.08891013384321
13	21.860241395793498	23.505497131931165	29.0663240917782	25.56793738049713
14	19.495458891013385	26.08138145315488	27.402127151051626	27.021032504780113
15	19.60635755258126	28.230640535372846	28.301206978967496	23.861794933078396
16	23.12649378585086	32.75161328871893	22.748685468451242	21.373207456978967
17	23.162583652007648	33.06972992351817	21.230879541108987	22.5368068833652
18	22.837715105162523	27.2444431166348	23.52372131931166	26.394120458891013
19	21.66073135755258	28.10325047801147	25.12219168260038	25.113826481835567
20	25.37810707456979	23.70022705544933	26.50973948374761	24.411926386233272
21	25.6453154875717	25.273362810707457	25.138922084130016	23.942399617590823
22	24.9798637667304	28.40505497131931	24.045411089866157	22.56967017208413
23	25.938635277246654	27.00621414913958	23.872251434034418	23.182899139579348
24	24.571641969407267	28.140774378585085	23.596677820267686	23.69090583173996
25	23.96972149430924	27.202096389384472	24.804245730050756	24.02393638625553
26	25.282187963776238	27.565254262955445	25.76969439985462	21.382863373413695
27	25.451976384298742	27.86040209166245	24.498636124311275	22.188985399727528
28	25.434372986027448	26.313839666128274	25.1902848882251	23.06150245961918
29	24.74198364875881	28.919727009237008	25.157568417784233	21.18072092421995
30	24.956073014749496	27.817183987398874	22.924153984956412	24.30258901289522
31	25.856438234959583	27.25363516909451	24.140011273595842	22.749915322350063
32	23.272233097463317	28.3354406757714	26.037929624918437	22.354396601846844
33	24.688563389372923	26.61910537296191	25.079436026623398	23.612895211041767
34	23.174923682822648	27.025673169469002	28.423040848168036	21.37636229954032
35	22.21001500404355	26.50267966497037	26.053663508969922	25.233641822016157
36	21.71772984990933	26.555784737775824	26.755359092831917	24.97112631948293
37	23.812871586753904	27.799017074985365	24.800487894481584	23.587623443779147
38	23.76876941879323	28.064164854523476	24.42606955299728	23.740996173686018
39	23.264514638856287	28.38871864013472	25.83118246936194	22.515584251647056
40	23.714377548085867	29.68477323093059	25.342987160184734	21.25786206079881
41	22.371266313547753	28.53157592966745	26.000245727079125	23.096912029705674
42	22.092454171809166	29.896893023844907	27.219718563867612	20.790934240478315
43	23.630226636586404	27.099525256796703	24.805939949185742	24.46430815743115
44	22.567109562064637	29.516280773092053	24.323467282356514	23.593142382486796
45	23.560905359739895	28.061830139136294	25.107725071117738	23.26953943000607
46	23.5365492281047	30.764976149787394	23.456497097121286	22.241977524986623
47	25.02901577187066	27.71059168801307	24.13980744775883	23.12058509235744
48	23.202537123711974	27.6058727253914	25.396916509506283	23.794673641390343
49	22.037937576008552	29.122302770598623	25.48117606812985	23.358583585262977
50	20.921504515024214	30.893723756975138	26.7717134175749	21.413058310425754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16265.0
1	13439.5
2	9417.0
3	7421.5
4	6407.0
5	5925.0
6	5355.0
7	5108.5
8	4956.0
9	4733.5
10	4440.0
11	3840.0
12	3628.5
13	4138.0
14	4923.5
15	5686.0
16	5685.0
17	5499.5
18	5680.0
19	5976.0
20	6330.5
21	11241.5
22	16281.5
23	17702.5
24	19392.5
25	19513.0
26	19464.0
27	20714.0
28	20729.0
29	25741.5
30	33624.5
31	45207.5
32	55927.5
33	56051.5
34	56506.0
35	59404.0
36	64968.0
37	73555.0
38	77986.5
39	90839.5
40	108003.5
41	105980.0
42	99573.0
43	104825.0
44	110117.0
45	108143.0
46	106017.0
47	99402.5
48	98478.0
49	106508.0
50	114758.5
51	117706.5
52	112948.0
53	104048.5
54	94592.5
55	90580.5
56	85386.0
57	81434.0
58	81686.5
59	80134.0
60	75583.5
61	64757.0
62	56574.5
63	57014.5
64	54817.0
65	47165.5
66	42144.5
67	38727.0
68	33302.0
69	30153.0
70	26324.0
71	22839.0
72	21151.0
73	18843.5
74	17202.0
75	16040.5
76	14582.0
77	12245.0
78	8882.5
79	7221.0
80	6307.0
81	5219.5
82	4704.0
83	4394.0
84	3722.0
85	2419.0
86	1551.5
87	914.5
88	281.5
89	120.5
90	55.5
91	10.0
92	7.5
93	7.5
94	4.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11695.0
25	11055.0
26	11771.0
27	11388.0
28	10933.0
29	11822.0
30	13607.0
31	15885.0
32	13810.0
33	12507.0
34	10875.0
35	13507.0
36	11303.0
37	11997.0
38	12121.0
39	11499.0
40	12785.0
41	12277.0
42	12616.0
43	14754.0
44	18571.0
45	16478.0
46	21261.0
47	45064.0
48	102117.0
49	261885.0
50	960017.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.78762342422995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12664195100302	7.45712181948997
2	13.610953376761707	2.936596789465127
3	5.520340122179297	1.7865405123521343
4	2.789518645479108	1.2036910672918655
5	1.6070984006448277	0.8668386175919317
6	1.0674923517035948	0.690942329905441
7	0.7564431247391261	0.5712156500073448
8	0.5133533535908504	0.4430290129682928
9	0.4325780330941271	0.41998400303521727
>10	2.808597618616386	6.029867007926447
>50	0.4572456066188099	3.514502667632103
>100	0.6969397321223648	18.569422714631358
>500	0.5131557836993107	41.09937158634382
>1k	0.09853476752802298	13.419401057973976
>5k	5.535661097079943E-4	0.37030280600795945
>10k+	5.535661097079943E-4	0.6211723573770028
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	10402	0.6215344168260039	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	6201	0.3705186424474187	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	4997	0.2985779158699809	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3591	0.2145673996175908	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3156	0.1885755258126195	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	3071	0.18349665391969408	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	2715	0.16222514340344169	No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	2478	0.1480640535372849	No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG	2253	0.1346199808795411	No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCG	2170	0.12966061185468453	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	2011	0.12016013384321224	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2008	0.11998087954110898	No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC	1947	0.11633604206500957	No Hit
AACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAAT	1933	0.11549952198852773	No Hit
AGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGAGCAGACGCTGTGTAT	1899	0.11346797323135754	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT	1857	0.11095841300191205	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	1728	0.10325047801147226	No Hit
CATTATAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATA	1699	0.1015176864244742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03352055449330784	0.0	0.0	0.0	0.0
2	0.06596558317399617	0.0	0.0	0.0	0.0
3	0.093032982791587	0.0	0.0	0.0	0.0
4	0.12954110898661567	0.0	0.0	0.0	0.0
5	0.16748326959847037	0.0	0.0	0.0	0.0
6	0.22932600382409177	0.0	0.0	0.0	0.0
7	0.29881692160611856	0.0	0.0	0.0	0.0
8	0.368546845124283	0.0	0.0	0.0	0.0
9	0.43773900573613767	0.0	0.0	0.0	0.0
10	0.5101577437858509	0.0	0.0	0.0	0.0
11	0.5876553537284895	0.0	0.0	0.0	0.0
12	0.6664077437858509	0.0	0.0	0.0	0.0
13	0.7389459847036329	0.0	0.0	0.0	0.0
14	0.8231955066921606	0.0	0.0	0.0	0.0
15	0.9071462715105163	0.0	0.0	0.0	0.0
16	0.9913957934990439	0.0	0.0	0.0	0.0
17	1.0931524856596557	0.0	0.0	0.0	0.0
18	1.1723231357552581	0.0	0.0	0.0	0.0
19	1.2687021988527725	0.0	0.0	0.0	0.0
20	1.365678776290631	0.0	0.0	0.0	0.0
21	1.4540511472275335	0.0	0.0	0.0	0.0
22	1.5414077437858509	0.0	0.0	0.0	0.0
23	1.6134082217973231	0.0	0.0	0.0	0.0
24	1.6891730401529637	0.0	0.0	0.0	0.0
25	1.7638025812619502	0.0	0.0	0.0	0.0
26	1.8457815487571703	0.0	0.0	0.0	0.0
27	1.914555449330784	0.0	0.0	0.0	0.0
28	1.9816563097514341	0.0	0.0	0.0	0.0
29	2.068833652007648	0.0	0.0	0.0	0.0
30	2.144180210325048	0.0	0.0	0.0	0.0
31	2.222633843212237	0.0	0.0	0.0	0.0
32	2.2942160611854683	0.0	0.0	0.0	0.0
33	2.365678776290631	0.0	0.0	0.0	0.0
34	2.447119980879541	0.0	0.0	0.0	0.0
35	2.5249760994263863	0.0	0.0	0.0	0.0
36	2.5969168260038242	0.0	0.0	0.0	0.0
37	2.6729804015296366	0.0	0.0	0.0	0.0
38	2.748386711281071	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACAT	225	0.0	71.53309	44
AATATAC	50	0.0	71.53309	44
ACTACCC	95	0.0	71.53309	44
TTAGTCC	80	0.0	71.53309	44
CGGTCTT	130	0.0	71.53309	44
ATTATGC	95	0.0	71.53309	44
TATACAA	20	7.12129E-5	71.53309	44
CGATATA	70	0.0	71.53309	44
GTAGTTA	25	2.4976453E-6	71.53309	44
CGGATTA	155	0.0	71.53308	44
TGTCCGA	15	0.0020416074	71.53308	44
GCCACTC	110	0.0	68.281586	44
TTCGTAG	85	0.0	67.32526	44
CCCGAAC	135	0.0	66.23434	44
TAACTCC	65	0.0	66.03054	44
ATCCTCG	50	1.0913936E-11	64.379776	44
GTTCGGG	100	0.0	64.379776	44
ACGCTTA	50	1.0913936E-11	64.379776	44
GAATTTC	85	0.0	63.117428	44
GCCAATA	190	0.0	62.12084	44
>>END_MODULE