##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840900.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9318896
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.31519184246718	38.0	35.0	39.0	30.0	39.0
2	37.03047903957722	39.0	37.0	39.0	33.0	39.0
3	37.19362583293128	39.0	37.0	39.0	33.0	39.0
4	37.588981784966805	39.0	37.0	40.0	33.0	41.0
5	37.964701290796675	39.0	37.0	41.0	33.0	41.0
6	37.92924719838058	39.0	37.0	40.0	33.0	41.0
7	37.81479758975741	39.0	37.0	40.0	33.0	41.0
8	37.61036908234623	39.0	36.0	40.0	33.0	41.0
9	37.464415741950546	39.0	36.0	40.0	32.0	41.0
10	37.367755150395496	39.0	36.0	40.0	32.0	41.0
11	37.39097313673208	39.0	36.0	40.0	32.0	41.0
12	37.562150387771254	39.0	36.0	40.0	33.0	41.0
13	37.62756564726122	39.0	36.0	41.0	33.0	41.0
14	37.65078105818543	39.0	36.0	41.0	33.0	41.0
15	37.584600901222636	39.0	35.0	41.0	33.0	41.0
16	37.51459829576379	39.0	35.0	41.0	33.0	41.0
17	37.51470367305312	39.0	35.0	41.0	33.0	41.0
18	37.384682906644734	39.0	35.0	41.0	32.0	41.0
19	37.21226205335911	39.0	35.0	40.0	32.0	41.0
20	37.124810170646825	39.0	35.0	40.0	32.0	41.0
21	36.89763057769933	38.0	35.0	40.0	32.0	41.0
22	36.7361852734487	38.0	35.0	40.0	31.0	41.0
23	36.56977156950781	38.0	35.0	40.0	31.0	41.0
24	36.34236544758092	37.0	34.0	40.0	31.0	41.0
25	36.10050423827555	37.0	34.0	40.0	30.0	41.0
26	35.891518974735085	37.0	34.0	40.0	30.0	41.0
27	35.81676785367881	37.0	34.0	40.0	30.0	41.0
28	35.72757076740233	36.0	34.0	40.0	30.0	41.0
29	35.62304784402634	36.0	34.0	40.0	30.0	41.0
30	35.521395243812655	36.0	34.0	39.0	30.0	41.0
31	35.28685359679952	35.0	33.0	39.0	29.0	41.0
32	35.243279909748736	35.0	33.0	39.0	29.0	41.0
33	35.18684229236502	35.0	33.0	39.0	30.0	41.0
34	35.05424027784586	35.0	33.0	39.0	30.0	41.0
35	34.91530769373706	35.0	33.0	39.0	29.0	40.0
36	34.83112861674439	35.0	33.0	39.0	29.0	40.0
37	34.76707045844226	35.0	33.0	38.0	29.0	40.0
38	34.62744481086085	35.0	33.0	38.0	29.0	40.0
39	34.50708086726787	35.0	33.0	37.0	29.0	40.0
40	34.36546326477938	35.0	33.0	37.0	29.0	40.0
41	34.263347150470935	35.0	33.0	37.0	29.0	39.0
42	34.09059292218829	35.0	33.0	37.0	29.0	39.0
43	34.02995968869108	35.0	33.0	36.0	29.0	39.0
44	33.90576145472756	35.0	33.0	36.0	29.0	39.0
45	33.7225717242464	35.0	33.0	36.0	28.0	39.0
46	33.579285478303945	35.0	33.0	35.0	28.0	39.0
47	33.50248886998031	35.0	33.0	35.0	28.0	38.0
48	33.41718749530954	35.0	32.0	35.0	28.0	37.0
49	33.48103802068713	35.0	32.0	36.0	28.0	38.0
50	33.94681218099206	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	704.0
14	4056.0
15	6125.0
16	573.0
17	3.0
18	1.0
19	6.0
20	25.0
21	121.0
22	320.0
23	908.0
24	2405.0
25	6202.0
26	14485.0
27	32246.0
28	66573.0
29	127186.0
30	220818.0
31	352635.0
32	523843.0
33	746035.0
34	1001859.0
35	1152003.0
36	1334516.0
37	1365254.0
38	1096298.0
39	958894.0
40	304802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.291231922751365	18.118262077396295	41.59050599985235
2	25.689995896509632	19.370320261112475	30.714067417427987	24.22561642494991
3	24.562834481681094	15.314303325200754	27.13392230152585	32.98893989159231
4	18.686623394015772	16.896218178634033	33.47031665553516	30.94684177181503
5	20.704104863923796	17.940977128621245	34.7563702824884	26.598547724966565
6	26.794493682513465	19.726285173694393	28.161468912197325	25.317752231594813
7	31.603883120919047	17.553441952780673	22.49622702088316	28.346447905417122
8	25.11134366130924	16.244617388154133	28.449721941311502	30.194317009225124
9	24.8789448878923	19.325314930008876	28.172768533955093	27.62297164814373
10	29.252993058405202	16.02548198842438	25.909946843488758	28.811578109681662
11	29.72568853649617	18.932371388198774	24.350620502686155	26.991319572618906
12	26.494941031641517	17.25666860108751	25.772806134975646	30.475584232295326
13	24.98398951978861	17.21280074377909	26.54497914774454	31.25823058868776
14	25.615824020356058	19.119432173081446	22.04589470684081	33.21884909972168
15	24.33365497372221	20.901520952696544	25.570099719966827	29.194724353614422
16	27.487687382711428	21.483607071052194	22.774929562471776	28.2537759837646
17	26.730526877861926	19.57411049549217	20.080576068238127	33.61478655840778
18	26.050628744005728	20.76021666085768	20.672588255089444	32.51656634004715
19	26.084752957861102	21.28168401063817	24.99992488380598	27.63363814769475
20	32.20559602768397	17.29878732416372	22.474175052495486	28.021441595656825
21	30.516350863879154	21.447583490576566	21.68446777386506	26.35159787167922
22	27.81475402236488	23.14928721170405	22.470269010406383	26.56568975552469
23	32.606051188896195	20.60711912655748	20.47299379669008	26.313835887856246
24	31.17182550379358	20.382607553512777	19.830825454002277	28.614741488691365
25	30.05527308578188	22.629098216889147	22.771106011486193	24.54452268584278
26	29.907968116708332	20.942235829468586	24.277149427483653	24.872646626339424
27	28.78470278509076	23.65130109463766	21.016034363308854	26.547961756962728
28	28.97303012714767	20.295171712233113	24.725412613175155	26.00638554744406
29	34.60062648335775	20.182021200254795	22.482500879173585	22.73485143721387
30	30.922095976372766	22.79177629203431	19.924000708194466	26.362127023398457
31	26.65419180111593	20.72911859928334	27.211076398037665	25.405613201563064
32	25.919284271846077	19.35949298670717	27.453306526912158	27.267916214534594
33	29.331617452806746	19.286642169713804	25.58658217572034	25.79515820175911
34	26.465255331193838	19.025996868611433	28.318378021458372	26.190369778736354
35	26.277151170903828	19.733932582959643	24.364777947821025	29.624138298315504
36	26.218955301632914	18.85611642909665	28.668678642643908	26.256249626626527
37	24.2237040567976	20.193710202895797	25.826498605384955	29.756087134921643
38	26.843977664905932	22.476521327082164	23.095790621257525	27.58371038675438
39	27.099258106397627	22.664348238556176	23.491796011577552	26.744597643468644
40	24.892982288649186	24.309907852185734	22.084569918109416	28.71253994105566
41	28.42249578108716	20.562067666426493	22.627658331804295	28.38777822068205
42	26.44015163783915	22.39312013605186	24.91133397840074	26.255394247708256
43	26.199103765542333	20.313997409856764	26.045223815421025	27.441675009179882
44	25.476694650005243	22.03412816842789	25.53160560749561	26.957571574071252
45	26.65487140185804	22.04448879895491	27.35496328754681	23.945676511640247
46	24.330942829024437	25.28318061713729	22.78179777592515	27.60407877791312
47	29.0842561383864	19.54760468318713	23.44326515054867	27.9248740278778
48	24.56364465978032	21.290649399771254	22.62910396460166	31.516601975846758
49	24.485776750220108	20.00237178297915	22.787901275790965	32.723950191009784
50	23.969604647198082	30.58022952602736	21.349168257161274	24.100997569613284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5490.0
1	4731.0
2	3939.0
3	3276.5
4	2551.5
5	2067.0
6	1439.0
7	1641.0
8	2441.5
9	3244.0
10	3975.5
11	4480.0
12	4618.0
13	4620.0
14	5026.0
15	5611.0
16	5821.0
17	5928.0
18	6418.5
19	8385.0
20	10220.5
21	11293.5
22	12906.5
23	14395.5
24	14774.5
25	16018.0
26	17319.0
27	18260.5
28	23104.5
29	32087.0
30	40947.0
31	50240.5
32	62403.5
33	69030.0
34	84772.5
35	124818.5
36	146953.5
37	139092.0
38	136915.5
39	162843.0
40	206015.0
41	259457.5
42	331415.0
43	486500.5
44	605310.0
45	528873.5
46	470115.5
47	488851.5
48	510638.5
49	589746.5
50	708820.5
51	754659.0
52	743723.5
53	765634.0
54	769129.5
55	754004.0
56	745151.5
57	744295.5
58	742177.0
59	711951.0
60	674718.5
61	640899.0
62	603844.5
63	573994.0
64	557891.5
65	544170.0
66	499830.0
67	440971.5
68	381249.0
69	338589.0
70	306274.5
71	277332.5
72	268157.0
73	250603.0
74	221552.5
75	196274.5
76	176684.0
77	143472.0
78	112289.0
79	97909.0
80	91356.5
81	86335.5
82	76122.0
83	70530.5
84	62871.5
85	46887.5
86	34609.5
87	20028.0
88	7905.0
89	5071.5
90	3147.0
91	1860.5
92	1235.5
93	1123.5
94	696.0
95	216.5
96	149.5
97	135.5
98	71.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	96867.0
25	94866.0
26	105913.0
27	91397.0
28	97695.0
29	94445.0
30	130005.0
31	100791.0
32	96075.0
33	87378.0
34	89611.0
35	98567.0
36	87904.0
37	95591.0
38	92443.0
39	87270.0
40	92967.0
41	90240.0
42	105115.0
43	95431.0
44	95568.0
45	128160.0
46	164834.0
47	337303.0
48	1493353.0
49	2946004.0
50	2223103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.872002367716797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.7760965604748	4.451414889350347
2	12.894898880642655	1.7722755127849001
3	5.604721729557723	1.1554698298774337
4	3.3261573461086997	0.9142944463143041
5	2.0437298753506865	0.7022258271191735
6	1.4217650212352713	0.5862223555359423
7	1.082088404252705	0.5205279853272435
8	0.8300290725896603	0.45631694016879387
9	0.6608339811652446	0.40871274149117504
>10	4.85339771637805	6.794305955769528
>50	0.7361849657831057	3.578513050505479
>100	1.0064267460210947	16.88737873864078
>500	0.5981636929845103	29.511148353508982
>1k	0.1500483709104971	17.76065556081034
>5k	0.009212126830092954	4.488088494503259
>10k+	0.0062455097153172575	10.012449318292266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	213634	2.2924818562198785	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	36946	0.3964632720442422	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	33050	0.35465574462897753	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	32157	0.345073064448836	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	31972	0.34308785074970255	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	30744	0.3299103241414004	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	30032	0.3222699341209517	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	27524	0.29535687489161805	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	26138	0.2804838684754074	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	23050	0.2473468960271689	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	22852	0.24522218082485306	No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG	22049	0.23660528028212782	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	20538	0.22039091325839455	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	20315	0.21799792593457423	No Hit
CCGATCGCACGCCCCCCGTGGCGGCG	18682	0.20047439095789887	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	17874	0.19180383599087272	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	17002	0.18244650439279503	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	16561	0.17771418416945528	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	16364	0.17560019985199965	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	15909	0.17071764724061733	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	15838	0.1699557544155445	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	15771	0.1692367851299124	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	15507	0.16640383152682464	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	15379	0.16503027826472147	No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG	14541	0.15603779675188992	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	14096	0.15126255298910943	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC	13900	0.149159299556514	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	13722	0.1472492020514018	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	13433	0.14414797632680953	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	13021	0.13972685176441502	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	12593	0.1351340330442576	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	12437	0.13346001500606938	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	11963	0.12837357558234366	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	11641	0.12491823065736542	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	11620	0.12469288207530163	No Hit
CTTGTTTCCTGAGTTGTCCTGTGCTGGAGGTCTGCTCAGACGAAGGTCT	11354	0.12183846670249351	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	11318	0.121452154847527	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	10718	0.1150136239314185	No Hit
CCGATCGCACGCCCCCCGTGGCGG	10485	0.11251332775899636	No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAGC	10415	0.11176216581878368	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	9987	0.10716934709862627	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	9881	0.1060318733034471	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	9869	0.10590310268512493	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	9512	0.10207217679004037	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	9420	0.10108493538290374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.009496833101260064	0.0	0.0	0.0	0.0
2	0.018081540989404753	0.0	0.0	0.0	0.0
3	0.027953955060771148	0.0	0.0	0.0	0.0
4	0.03931796212770268	0.0	0.0	0.0	0.0
5	0.05280668439695003	0.0	0.0	0.0	0.0
6	0.07820668886099813	0.0	0.0	0.0	0.0
7	0.09740424187586169	0.0	0.0	0.0	0.0
8	0.1201644486643053	0.0	0.0	0.0	0.0
9	0.14228080236113805	0.0	0.0	0.0	0.0
10	0.1654058592348278	0.0	0.0	0.0	0.0
11	0.19175018156657184	0.0	0.0	0.0	0.0
12	0.2143172324275322	0.0	0.0	0.0	0.0
13	0.2403288973286106	0.0	0.0	0.0	0.0
14	0.26827212150452157	0.0	0.0	0.0	0.0
15	0.30007846423009765	0.0	0.0	0.0	0.0
16	0.3290303915828656	0.0	0.0	0.0	0.0
17	0.36258586854065117	0.0	0.0	0.0	0.0
18	0.39273965499775937	0.0	0.0	0.0	0.0
19	0.4256405479790739	0.0	0.0	0.0	0.0
20	0.4582731688388839	0.0	0.0	0.0	0.0
21	0.4906697102317699	0.0	0.0	0.0	0.0
22	0.522057548447799	0.0	0.0	0.0	0.0
23	0.5509343596065457	0.0	0.0	0.0	0.0
24	0.5825153537500579	0.0	0.0	0.0	0.0
25	0.6159635218592417	0.0	0.0	0.0	0.0
26	0.6482312926338055	0.0	0.0	0.0	0.0
27	0.6828705889624693	0.0	0.0	0.0	0.0
28	0.7155890569011608	0.0	0.0	0.0	0.0
29	0.7542524350523925	0.0	0.0	0.0	0.0
30	0.7881513003257038	0.0	0.0	0.0	0.0
31	0.8215994684348875	0.0	0.0	0.0	0.0
32	0.856453382460755	0.0	0.0	0.0	0.0
33	0.8918009171901907	0.0	0.0	0.0	0.0
34	0.9293912068554043	0.0	0.0	0.0	0.0
35	0.9696534868508029	0.0	0.0	0.0	0.0
36	1.0098084579975997	0.0	0.0	0.0	0.0
37	1.0460466561704305	0.0	0.0	0.0	0.0
38	1.0767047942159673	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATT	60	0.0	152.88026	44
TAAGCCA	24315	0.0	150.96829	44
AATCGTT	35	1.1996963E-7	119.127495	44
CTATCAT	4895	0.0	116.0124	44
ATACTAT	165	0.0	111.18565	44
CGACGGG	6765	0.0	94.79131	44
AATACGG	100	0.0	91.72816	44
TTCTATA	305	0.0	87.49035	44
AATGAAG	3535	0.0	86.33794	44
GAACACA	7535	0.0	76.251076	44
CGTACAG	495	0.0	75.8084	44
ACATTTG	700	0.0	73.85904	44
TATAATT	200	0.0	70.88085	44
GTTCGAA	145	0.0	69.01178	44
TTAAGCC	23160	0.0	68.697296	43
CAATCGT	160	0.0	67.75376	44
ACTTCTA	2890	0.0	66.65368	44
ATAGTGT	285	0.0	64.37064	44
ATGCTAC	205	0.0	61.016518	44
GACACCG	395	0.0	59.111362	44
>>END_MODULE