FastQCFastQC Report
Tue 24 May 2016
ERR840899.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840899.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822510
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA4288516.285824425321472No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG407250.5969210745019062No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC359600.5270787437468029No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT342200.501574933565506No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC330230.48403007104423446No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC237050.34745277031473754No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT236300.3463534681517506No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG189800.27819673404656053No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA187190.27437116251936605No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA170320.24964419253324655No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA167150.24499780872435512No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG138010.20228625535176936No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG111720.16375205019853398No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT111220.16301918208987604No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC104700.15346258195297627No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCTG101990.14949043680405014No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC100370.14711594413199836No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC99930.14647102019637934No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT99630.14603129933118456No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG98920.14499062661689027No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC97900.14349557567522803No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG95560.14006575292670878No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC92890.13615223722647532No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG89850.13169639912583492No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT86010.1260679720513418No Hit
CCGATCGCACGCCCCCCGTGGCGGCG85630.12551099228876175No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG85590.12545236284006914No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG85230.12492469780183539No Hit
ACTTGTGTTTGGGAACAGATCAGGACTCAGGATGCAAGACCCTGGTCAT85220.12491004043966222No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA83100.12180267965895249No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG79190.11607165104924727No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG78240.11467920164279716No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC75530.11070705649387103No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT75500.11066308440735155No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG75410.11053116814779311No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT74700.1094904954334988No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG71060.10415521560246889No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG69830.10235236005517032No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC69180.10139963151391497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1050.0111.2816644
TAAGCCA481650.0109.5921644
GACCGTA800.0102.2400344
CTATCAT46150.095.1982744
TATGTCT1750.090.13814544
TATGGAT700.083.4612544
GATCTAA1500.074.00230444
CGACGGG71750.072.9573544
GTCAACT3200.067.55144544
TCGTCGA150.0029304665.3047543
TACGTTA900.065.3047543
ACCTAAG1800.064.9143144
GTAATGT1200.063.29144744
TTAAGCC475000.062.49320643
ACTTCTA31250.062.2554144
TCGACAA1600.062.07430344
AACGACA11650.061.3808643
GCCAGTT2150.059.78154444
ACGACAC8850.059.41309444
GAACACA38100.059.03623644
Produced by FastQC (version 0.11.5)