FastQCFastQC Report
Tue 24 May 2016
ERR840898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4250881
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA350080.8235469306244988No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA252250.5934064021081747No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC234830.552426661673192No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG130590.3072069060507692No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG102820.24187927161451941No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA102580.24131468276811324No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG85980.20226395422501828No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT85880.20202870887234903No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT82320.19365397431732387No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT77670.1827150654182039No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC74150.1744344290042464No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA70230.16521281117961192No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG67720.15930815282761385No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC66630.15674397848351906No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA60140.14147655509528495No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA58980.13874770900432168No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC58590.13783025212891165No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC56060.1318785447063797No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG55990.13171387295951123No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA53990.1270089659061263No Hit
AACTTGGACTCTTCTAGAGGAGAGCAGGCACAAACTAAGCCAAGAAAAG51310.12070439045459046No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT49410.11623472875387478No Hit
CTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGCC48940.11512907559632932No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG48880.11498792838472778No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48230.11345883359237768No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA45010.10588393323642793No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT44360.10435483844407784No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT43980.10346090610393469No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG43520.10237877748165616No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT42900.10092025629510681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG154.6515948E-4103.81132544
GGGTAAG1000.093.4301944
AAACGAG950.092.8838144
TCGTTCA1000.088.23962444
TAAGCCA45950.078.3950644
GAACACA41550.064.8352344
TACGGGC1150.063.189544
GTTCCGT650.061.18224343
TTATCGA404.474714E-1061.1822443
AACCTAT2150.057.94120444
CCATTAG2350.057.4275444
CCGCTTA1450.057.2752144
GTGCGTA650.056.47591843
CGTACGA551.8189894E-1255.6202243
TTAAGCC40000.054.4521943
AGCTAAC2900.053.6955144
GACGAAC403.0900992E-853.5344643
CCGTTCG1150.053.2019543
AACTCCG750.053.0246143
CGTTACA2350.053.01003644
Produced by FastQC (version 0.11.5)