##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840898.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4250881
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.676344268399895	39.0	37.0	39.0	32.0	39.0
2	37.22366869361904	39.0	37.0	39.0	34.0	39.0
3	37.22132964907745	39.0	37.0	39.0	33.0	39.0
4	37.584689385565014	39.0	37.0	39.0	33.0	41.0
5	38.12309495372841	39.0	37.0	40.0	33.0	41.0
6	38.05163659015625	39.0	37.0	40.0	33.0	41.0
7	38.058061611228354	39.0	37.0	40.0	33.0	41.0
8	37.932080197022685	39.0	37.0	40.0	33.0	41.0
9	37.81525782537785	39.0	37.0	40.0	33.0	41.0
10	37.78850995828865	39.0	37.0	40.0	33.0	41.0
11	37.79270485341745	39.0	37.0	40.0	33.0	41.0
12	38.03819208300585	39.0	37.0	40.0	34.0	41.0
13	38.1631760569162	39.0	37.0	41.0	34.0	41.0
14	38.20125686887024	40.0	37.0	41.0	34.0	41.0
15	38.191619807752794	40.0	37.0	41.0	34.0	41.0
16	38.17782549076297	40.0	37.0	41.0	34.0	41.0
17	38.20483824411928	40.0	37.0	41.0	34.0	41.0
18	38.24135373349666	40.0	37.0	41.0	34.0	41.0
19	38.216885393874826	40.0	37.0	41.0	34.0	41.0
20	38.206707033200885	40.0	37.0	41.0	34.0	41.0
21	38.06645587114765	40.0	37.0	41.0	33.0	41.0
22	37.98667993764116	40.0	37.0	41.0	33.0	41.0
23	37.8766683894468	40.0	37.0	41.0	33.0	41.0
24	37.77872963275142	39.0	36.0	41.0	33.0	41.0
25	37.675996681740536	39.0	36.0	41.0	33.0	41.0
26	37.581576626390685	39.0	36.0	41.0	32.0	41.0
27	37.54093685135738	39.0	36.0	41.0	32.0	41.0
28	37.51787153480766	39.0	36.0	41.0	32.0	41.0
29	37.419974064692006	39.0	35.0	41.0	32.0	41.0
30	37.434328527221176	39.0	35.0	41.0	32.0	41.0
31	37.26595611545242	39.0	35.0	41.0	31.0	41.0
32	37.147906248619165	39.0	35.0	40.0	31.0	41.0
33	37.130195483449114	39.0	35.0	40.0	31.0	41.0
34	37.09601012723534	39.0	35.0	40.0	31.0	41.0
35	37.03598095051417	39.0	35.0	40.0	31.0	41.0
36	36.95939321424982	39.0	35.0	40.0	31.0	41.0
37	36.88802578000174	38.0	35.0	40.0	31.0	41.0
38	36.80388271315183	38.0	35.0	40.0	31.0	41.0
39	36.75106533374269	38.0	35.0	40.0	31.0	41.0
40	36.60594426921433	38.0	35.0	40.0	31.0	41.0
41	36.59039834970349	38.0	35.0	40.0	31.0	41.0
42	36.47308309744873	38.0	34.0	40.0	31.0	41.0
43	36.38594737577015	38.0	34.0	40.0	30.0	41.0
44	36.28130494021258	38.0	34.0	40.0	30.0	41.0
45	36.10124899940647	37.0	34.0	40.0	30.0	41.0
46	36.00439752299926	37.0	34.0	40.0	30.0	41.0
47	35.924948367817606	37.0	34.0	40.0	30.0	41.0
48	35.877896728582506	37.0	34.0	40.0	30.0	41.0
49	35.95462144377734	37.0	34.0	40.0	30.0	41.0
50	36.30694229476463	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	224.0
15	3409.0
16	4743.0
17	161.0
18	0.0
19	1.0
20	10.0
21	29.0
22	98.0
23	227.0
24	529.0
25	1193.0
26	2433.0
27	5379.0
28	11526.0
29	24028.0
30	46371.0
31	84056.0
32	139330.0
33	215077.0
34	297482.0
35	353241.0
36	464820.0
37	567078.0
38	654228.0
39	794708.0
40	580493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.411521988030245	15.383752215129052	35.204725796840705
2	25.838126261356177	24.08317240590833	29.905471359936918	20.17322997279858
3	23.92113540698975	18.492025535412544	28.47063938040138	29.116199677196324
4	20.519840475421447	19.66806410247664	35.45436816509331	24.35772725700861
5	21.143687626165022	21.963235385794142	31.260837459340777	25.63223952870005
6	22.01282510613682	24.808198582834947	29.242267661691777	23.936708649336456
7	29.926666966212416	22.418623339491273	22.87951603444086	24.77519365985545
8	23.393574179093697	21.969986927415754	27.806094783646024	26.83034410984452
9	22.74460282468505	23.703039440530095	28.664034584830766	24.888323149954093
10	25.137753797389294	21.20459264797109	25.84346633086177	27.814187223777846
11	23.715648591433165	25.593823962609164	25.075013861832407	25.615513584125267
12	26.788282240787264	22.43478469521965	25.90519471140218	24.87173835259091
13	23.49600000564589	22.907039740703162	26.47117150538912	27.12578874826183
14	24.83868638054088	23.762391843008544	24.27492089286903	27.124000883581544
15	22.968297630538235	25.16762995717829	26.230327313326345	25.633745098957135
16	24.794836646803333	28.893539950894887	23.383787972422656	22.927835429879124
17	25.225570887540723	25.993129424229945	21.732295023078745	27.04900466515059
18	24.89881509268314	23.69045381416229	21.807996977567708	29.602734115586866
19	24.850801516203347	24.32481643217018	23.4331894964832	27.391192555143274
20	27.57108467632945	22.094267047230915	25.475918991851337	24.858729284588303
21	26.339857549529142	26.572162335290027	22.548267053347296	24.539713061833535
22	27.039783047325955	26.71493273982499	22.406131811264533	23.839152401584517
23	26.494437270768106	25.577309738851785	23.8739451892443	24.05430780113581
24	27.09697119255985	25.965793914249772	21.940440111120495	24.99679478206988
25	26.872540454037907	26.96268476321581	22.1987715228434	23.966003259902884
26	26.36164373468899	26.450725969983306	23.19104336013216	23.996586935195545
27	26.36158402282619	26.297043969027527	23.076483220913005	24.26488878723328
28	26.580921001401652	24.675357226288742	23.840766625136272	24.902955147173337
29	27.472922291750617	25.475574721811427	24.57529951501367	22.476203471424284
30	28.42656775550046	25.37010096494759	23.00730460478884	23.196026674763115
31	25.26307822316567	26.05457978551072	24.19113141558778	24.491210575735828
32	24.041484922894348	24.532032142609157	26.678264602099492	24.748218332397
33	24.92789837327314	23.601223345077187	26.950795576917493	24.52008270473218
34	24.89515243652557	25.02852588552288	26.878231402805547	23.198090275146008
35	23.8316918604621	24.79254074902603	25.486439634712006	25.88932775579986
36	24.434965706964125	25.281640454895655	25.284754672897197	24.998639165243024
37	23.731193384626646	25.550732844004408	24.67265702224175	26.0454167491272
38	25.033658359648374	25.777476103098063	24.66458991209354	24.524275625160026
39	25.02746579321453	27.01460886907662	24.609835054558918	23.34809028314993
40	23.85280453344032	27.556964282413677	23.600478611960078	24.989752572185925
41	24.871612507394833	25.72927608002994	25.086158736221464	24.312952676353763
42	24.097043170000745	26.397351180633503	24.706134126973787	24.799471522391965
43	24.352403228492744	25.705440693297117	26.23217647359003	23.709979604620113
44	23.776423727257374	27.02976278863625	25.561034856819642	23.632778627286733
45	24.986915039010572	25.88275702499292	25.756721760987126	23.373606175009375
46	23.193211312921335	26.001354701016904	25.31965396731784	25.485780018743924
47	23.949527334414203	24.834493592089288	24.30978775233291	26.906191321163604
48	24.463718984309697	25.078133201843674	24.80526766685735	25.652880146989283
49	21.988067351719298	26.339477562497716	23.880555702429717	27.791899383353268
50	21.1346391526307	31.650716225673303	25.487103620197406	21.727541001498597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21943.0
1	17778.5
2	11781.5
3	8452.5
4	6681.0
5	5924.5
6	5284.0
7	5127.5
8	5431.0
9	6010.0
10	5793.0
11	5998.0
12	6937.5
13	8038.5
14	9767.0
15	11674.0
16	12406.5
17	14736.5
18	17040.0
19	16612.0
20	16444.5
21	20087.5
22	25028.0
23	28660.0
24	31173.0
25	32741.5
26	35746.5
27	46877.0
28	58354.0
29	66290.5
30	70112.5
31	76026.5
32	89967.5
33	98389.5
34	111866.0
35	138386.0
36	153969.0
37	151132.5
38	147840.5
39	157994.0
40	175396.0
41	188695.0
42	200525.0
43	222981.0
44	252991.5
45	251193.5
46	239997.0
47	232648.0
48	242242.0
49	289986.5
50	327709.5
51	319568.0
52	306412.0
53	311572.0
54	300361.5
55	281022.0
56	272544.0
57	266363.0
58	254213.0
59	240810.0
60	236623.0
61	233791.5
62	220399.0
63	208854.5
64	195110.0
65	177477.0
66	155168.5
67	135556.0
68	123536.5
69	109433.5
70	94380.0
71	81469.5
72	69622.5
73	60341.5
74	56606.5
75	53054.0
76	47047.0
77	36636.0
78	27609.5
79	22451.5
80	17843.5
81	13834.5
82	11347.5
83	10554.5
84	9619.5
85	7206.0
86	4560.5
87	3507.0
88	2035.5
89	933.0
90	736.0
91	533.5
92	334.5
93	309.5
94	207.0
95	96.0
96	85.0
97	84.5
98	42.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	35418.0
25	35056.0
26	35694.0
27	35273.0
28	34686.0
29	30182.0
30	41295.0
31	42802.0
32	36153.0
33	32243.0
34	35177.0
35	35718.0
36	36545.0
37	35199.0
38	32999.0
39	37469.0
40	34536.0
41	37093.0
42	40849.0
43	37658.0
44	49651.0
45	54075.0
46	55603.0
47	102330.0
48	449194.0
49	1150422.0
50	1667561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.263660501940068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.02898060168764	6.30197380587101
2	13.108251250058773	2.428607787093519
3	5.416129191712994	1.5051954620002874
4	2.9172885689924133	1.0809908355734512
5	1.7907677070705512	0.8294532038069624
6	1.1695787585320023	0.6500748329592606
7	0.8244096767837436	0.5345935952167119
8	0.6512610356683968	0.48264489060591265
9	0.5022387671815163	0.4187312487074238
>10	3.6263675239435536	6.758233372590004
>50	0.5503611242431901	3.598901872201035
>100	0.7476332027786775	16.978173530726554
>500	0.5411971661976003	36.91072142059364
>1k	0.12020967983957945	16.40301091061173
>5k	0.0038041037923917543	2.362578615317614
>10k+	0.0015216415169567018	2.756114616124914
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	35008	0.8235469306244988	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	25225	0.5934064021081747	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23483	0.552426661673192	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	13059	0.3072069060507692	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	10282	0.24187927161451941	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	10258	0.24131468276811324	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	8598	0.20226395422501828	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	8588	0.20202870887234903	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	8232	0.19365397431732387	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	7767	0.1827150654182039	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	7415	0.1744344290042464	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	7023	0.16521281117961192	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	6772	0.15930815282761385	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	6663	0.15674397848351906	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	6014	0.14147655509528495	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	5898	0.13874770900432168	No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC	5859	0.13783025212891165	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC	5606	0.1318785447063797	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	5599	0.13171387295951123	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	5399	0.1270089659061263	No Hit
AACTTGGACTCTTCTAGAGGAGAGCAGGCACAAACTAAGCCAAGAAAAG	5131	0.12070439045459046	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	4941	0.11623472875387478	No Hit
CTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGCC	4894	0.11512907559632932	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	4888	0.11498792838472778	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4823	0.11345883359237768	No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA	4501	0.10588393323642793	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	4436	0.10435483844407784	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	4398	0.10346090610393469	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	4352	0.10237877748165616	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	4290	0.10092025629510681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.028111819643974977	0.0	0.0	0.0	0.0
2	0.05709404709282617	0.0	0.0	0.0	0.0
3	0.07871309500312994	0.0	0.0	0.0	0.0
4	0.1029904153985962	0.0	0.0	0.0	0.0
5	0.13006715549082648	0.0	0.0	0.0	0.0
6	0.17944515501610137	0.0	0.0	0.0	0.0
7	0.22463578726386366	0.0	0.0	0.0	0.0
8	0.28053008305807664	0.0	0.0	0.0	0.0
9	0.3339778271845295	0.0	0.0	0.0	0.0
10	0.3861317218713015	0.0	0.0	0.0	0.0
11	0.44941272173932884	0.0	0.0	0.0	0.0
12	0.5050247231103387	0.0	0.0	0.0	0.0
13	0.5675999869203584	0.0	0.0	0.0	0.0
14	0.6308574622531188	0.0	0.0	0.0	0.0
15	0.7008194301369528	0.0	0.0	0.0	0.0
16	0.768828861593632	0.0	0.0	0.0	0.0
17	0.8525762071438838	0.0	0.0	0.0	0.0
18	0.9191271174140138	0.0	0.0	0.0	0.0
19	0.9974873443881398	0.0	0.0	0.0	0.0
20	1.0774472397604167	0.0	0.0	0.0	0.0
21	1.1498792838472778	0.0	0.0	0.0	0.0
22	1.2250636985603691	0.0	0.0	0.0	0.0
23	1.286274539324907	0.0	0.0	0.0	0.0
24	1.354048725428917	0.0	0.0	0.0	0.0
25	1.4195410316120352	0.0	0.0	0.0	0.0
26	1.488914886114196	0.0	0.0	0.0	0.0
27	1.5477262242815077	0.0	0.0	0.0	0.0
28	1.604208633457394	0.0	0.0	0.0	0.0
29	1.6811103392449707	0.0	0.0	0.0	0.0
30	1.7419918365157716	0.0	0.0	0.0	0.0
31	1.80564922894807	0.0	0.0	0.0	0.0
32	1.861472951136482	0.0	0.0	0.0	0.0
33	1.919578553245786	0.0	0.0	0.0	0.0
34	1.9815657036741325	0.0	0.0	0.0	0.0
35	2.0455994886707014	0.0	0.0	0.0	0.0
36	2.1099155680904733	0.0	0.0	0.0	0.0
37	2.166656747154296	0.0	0.0	0.0	0.0
38	2.2211160462972264	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	15	4.6515948E-4	103.811325	44
GGGTAAG	100	0.0	93.43019	44
AAACGAG	95	0.0	92.88381	44
TCGTTCA	100	0.0	88.239624	44
TAAGCCA	4595	0.0	78.39506	44
GAACACA	4155	0.0	64.83523	44
TACGGGC	115	0.0	63.1895	44
GTTCCGT	65	0.0	61.182243	43
TTATCGA	40	4.474714E-10	61.18224	43
AACCTAT	215	0.0	57.941204	44
CCATTAG	235	0.0	57.42754	44
CCGCTTA	145	0.0	57.27521	44
GTGCGTA	65	0.0	56.475918	43
CGTACGA	55	1.8189894E-12	55.62022	43
TTAAGCC	4000	0.0	54.45219	43
AGCTAAC	290	0.0	53.69551	44
GACGAAC	40	3.0900992E-8	53.53446	43
CCGTTCG	115	0.0	53.20195	43
AACTCCG	75	0.0	53.02461	43
CGTTACA	235	0.0	53.010036	44
>>END_MODULE