FastQCFastQC Report
Tue 24 May 2016
ERR840897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365196
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT361421.5280763201020127No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC232670.9837239704447326No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA162710.6879345305843575No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG140970.5960182581063049No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG134150.5671834384972746Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTG106500.45027980767767234No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA86520.36580477896969216No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT81890.3462292342791041No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG80090.3386188713324393No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG79870.3376887158611802No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC75470.3190856064359994No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA69950.29574716006622703No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCACTGCA69420.29350633097637574No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT64150.2712248794603069Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTG60650.2564269515084585No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT58140.24581472317727582No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG57230.2419672619097952No Hit
AGCAGTGGTATCAACGCAGAGTGCAG50930.21533099159646812No Hit
AAGCAGTGGTATCAACGCAGAGTGC50040.21156808991728382No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCACTGC49290.20839710535617345No Hit
AAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGC44340.187468607252845Illumina Single End Adapter 2 (96% over 31bp)
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT44210.1869189699289192No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC42290.17880124945247666No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT36810.1556319222592969No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGG36440.1540675698758158No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA36380.15381389111092694No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC33070.1398192792478932Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCA33020.1396078802771525No Hit
AGCAGTGGTATCAACGCAGAGTGCAGTGC32620.137916688511227No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC32250.13635233612774586No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT31030.131194201241673No Hit
CATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC31000.13106736185922857No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT30950.1308559628884879No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG30540.12912249132841422No Hit
AGCAGTGGTATCAACGCAGAGTGCAGT28360.11990549620412008No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGACT28340.11982093661582381No Hit
AAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA28220.11931357908604615Illumina Single End Adapter 2 (100% over 34bp)
AGCAGTGGTATCAACGCAGAGTGCAGTG27160.11483192090634348No Hit
ACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA26360.11144953737449244Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA26300.1111958586096036Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTAGATCGGAAGAGCTCGTATGCCGTCTT26200.1107730606681222Illumina Single End Adapter 2 (100% over 27bp)
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG26090.11030798293249269No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24990.10565720557619748Illumina Single End Adapter 1 (100% over 32bp)
AGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA24740.10460021072249404Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA24230.10244394122093899Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATGCCGTCTT24040.10164062513212437Illumina Single End Adapter 2 (96% over 27bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG23840.10079502924916159No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT23750.10041451110182835Illumina Single End Adapter 2 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA202.6713706E-587.1769344
TCGTAGG600.087.1769344
AAGGTTT900.082.3337744
ACTCGCA600.079.9121944
GTATATG1200.079.9121944
GGTCCCT800.076.27981644
TGTTATG401.880835E-976.27981644
CGTGCGC1350.074.26182644
CTATCAT42450.074.0336544
ATCCGAG1400.071.6096244
ATGTGTG950.068.823944
GAGCGCT1150.068.22542644
CGCTACG1100.067.3639944
GTTCGTA1500.066.8356544
AATCGCA1250.066.2544744
GAAAATC1850.065.9717444
GAACACA22800.064.4268244
TAGCGAA550.063.67223743
CACGCTT1000.063.67223743
GGGTACG201.267431E-463.67223443
Produced by FastQC (version 0.11.5)