##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840897.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2365196
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.7671080959041	38.0	35.0	39.0	30.0	39.0
2	36.63670156722741	39.0	35.0	39.0	32.0	39.0
3	36.81850891004382	39.0	35.0	39.0	33.0	39.0
4	36.92007300874854	38.0	35.0	39.0	33.0	40.0
5	37.78816301059194	39.0	36.0	40.0	33.0	41.0
6	37.7083759654591	39.0	36.0	40.0	33.0	41.0
7	37.22191480114122	38.0	35.0	40.0	32.0	41.0
8	37.51660242956609	39.0	36.0	40.0	33.0	41.0
9	37.37513592953819	39.0	35.0	40.0	33.0	41.0
10	36.46314554903695	38.0	34.0	40.0	30.0	41.0
11	36.93389723304115	38.0	35.0	40.0	32.0	41.0
12	37.320244918391545	39.0	35.0	40.0	33.0	41.0
13	37.6114588389292	39.0	35.0	40.0	33.0	41.0
14	37.63194001681045	39.0	35.0	41.0	33.0	41.0
15	37.60339439099339	39.0	35.0	41.0	33.0	41.0
16	37.65079511380875	39.0	35.0	41.0	33.0	41.0
17	37.56779903230007	39.0	35.0	41.0	33.0	41.0
18	37.52808562165673	39.0	35.0	41.0	33.0	41.0
19	37.451299173514585	39.0	35.0	41.0	33.0	41.0
20	37.41519772568531	39.0	35.0	41.0	33.0	41.0
21	37.16622216509752	39.0	35.0	40.0	32.0	41.0
22	37.02606676148615	38.0	35.0	40.0	32.0	41.0
23	36.59022212112654	38.0	35.0	40.0	31.0	41.0
24	36.55359513545601	38.0	35.0	40.0	31.0	41.0
25	36.52791479541293	38.0	35.0	40.0	31.0	41.0
26	36.51355980389515	38.0	35.0	40.0	31.0	41.0
27	36.355995803121	38.0	35.0	40.0	31.0	41.0
28	36.179734759256455	37.0	34.0	40.0	30.0	41.0
29	36.22225422409451	37.0	34.0	40.0	31.0	41.0
30	36.23165937272572	37.0	34.0	40.0	31.0	41.0
31	36.16017131306738	37.0	34.0	40.0	31.0	41.0
32	36.06908572885127	37.0	34.0	40.0	31.0	41.0
33	35.947929941910786	37.0	34.0	40.0	31.0	41.0
34	35.965745793426315	37.0	34.0	40.0	31.0	41.0
35	35.85463529490447	36.0	34.0	40.0	31.0	41.0
36	35.76508562014741	36.0	34.0	40.0	30.0	41.0
37	35.616141678950555	36.0	34.0	39.0	30.0	41.0
38	35.52791152332731	36.0	34.0	39.0	30.0	41.0
39	35.26733159758501	35.0	34.0	39.0	30.0	41.0
40	35.08836611803325	35.0	33.0	39.0	29.0	41.0
41	34.8815545792855	35.0	33.0	38.0	29.0	41.0
42	34.78719270271286	35.0	33.0	38.0	29.0	40.0
43	34.71313273204245	35.0	33.0	38.0	29.0	40.0
44	34.53353244003878	35.0	33.0	37.0	29.0	40.0
45	34.38103681088319	35.0	33.0	37.0	29.0	40.0
46	34.25560311302061	35.0	33.0	37.0	29.0	40.0
47	34.15188903337053	35.0	33.0	37.0	29.0	39.0
48	34.08309743469848	35.0	33.0	36.0	29.0	39.0
49	34.068078702879234	35.0	33.0	37.0	29.0	39.0
50	33.960835567616904	35.0	32.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	77.0
14	430.0
15	1900.0
16	179.0
17	4.0
18	0.0
19	2.0
20	19.0
21	47.0
22	134.0
23	349.0
24	753.0
25	1826.0
26	3755.0
27	8121.0
28	16321.0
29	30214.0
30	52117.0
31	83085.0
32	125166.0
33	184225.0
34	262592.0
35	240108.0
36	287048.0
37	327200.0
38	318357.0
39	274518.0
40	146649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	58.65429334397657	12.468776372021601	28.876930284001833
2	24.144552925000717	36.293398094703356	21.866602175887326	17.695446804408597
3	36.59180888180092	16.93030936970974	19.352687895633174	27.125193852856167
4	19.232317321693422	22.48836882862985	23.46828761760125	34.811026232075484
5	19.222804368010095	35.310308321170844	21.155582877698084	24.311304433120977
6	30.580763708377656	26.361747609923235	22.511834114382065	20.545654567317044
7	24.41907562840458	23.280819010348402	29.62219621545107	22.67790914579595
8	35.36510293438683	25.256934309038236	19.253203540002605	20.124759216572325
9	31.16591605938789	24.966429843446377	24.32779355283875	19.53986054432698
10	23.89379146590811	23.11284138819785	29.505630822984646	23.48773632290939
11	21.338950344918562	33.27508587026192	23.219766987598494	22.166196797221033
12	21.64281522546123	22.637235983825445	30.78691998464398	24.93302880606935
13	21.69139470893744	23.357134038785794	19.760603349574414	35.19086790270236
14	16.989289682546392	35.352165317377505	21.358483609815	26.300061390261103
15	18.366173458774664	36.57104950287418	20.88347012256067	24.179306915790487
16	20.931711367683693	31.365434407972952	16.584206974813082	31.118647249530273
17	31.02047356751829	29.22311723848679	15.27729625789998	24.47911293609494
18	21.572292528822135	28.041904349576104	17.953142149741502	32.43266097186026
19	23.81088078958361	31.375031921244585	20.593092496351254	24.22099479282055
20	31.35600601387792	23.642776328050612	23.146411544751473	21.854806113319995
21	24.09639623946599	34.55950373668821	18.8411023864407	22.5029976374051
22	29.880018400166414	25.653307379177033	20.77075219136173	23.695922029294824
23	26.48951714783891	22.656642409339437	27.217025565745928	23.636814877075725
24	27.83393004216141	25.694572458265615	22.104806536117938	24.36669096345504
25	21.990822085763064	26.59511582369675	21.149584880241193	30.264477210298992
26	23.852091938713322	32.262708865667825	22.629870779107303	21.25532841651155
27	26.47476730230462	26.376024997649516	22.20549787617971	24.943709823866154
28	23.349610905689037	23.696959598595335	25.95427698341089	26.999152512304743
29	26.3621502816063	26.37858876322176	22.903841750995923	24.355419204176016
30	23.717963901378305	25.192821517427667	25.26480883651308	25.824405744680952
31	23.351012826816316	26.193436452723134	24.580665396983278	25.874885323477276
32	24.144343306088707	25.96273735816874	25.493964652660818	24.39895468308173
33	20.626372126821284	25.06496280641252	28.12280755048872	26.185857516277466
34	21.789781563357433	27.893371649915334	25.471814289184085	24.845032497543155
35	20.774317853008547	27.20960744644963	24.307378488187396	27.70869621235443
36	20.941165833020342	26.93621562326738	26.061880602769016	26.060737940943262
37	21.528393087040158	30.64899058062561	21.649208329026866	26.17340800330737
38	24.354553467835256	26.63114799385055	21.963708506284537	27.05059003202966
39	23.60670770371382	28.118968758070533	24.6580146236197	23.616308914595947
40	21.226635173358996	28.62818734352284	24.78504567779347	25.360131805324706
41	20.302473266452076	29.37423793188268	22.393495608107212	27.929793193558027
42	20.310209866799813	31.557330842205296	24.177578773871073	23.954880517123822
43	21.582566166282195	26.56048845470208	24.31145972666314	27.545485652352582
44	21.23103508630581	29.253961065841345	22.4799619937712	27.035041854081644
45	20.793222033152066	27.50681814189555	28.615400132707986	23.0845596922444
46	20.903569973518028	30.157734402488934	23.874805551954665	25.06389007203837
47	21.59963053699272	28.34481400600673	25.14607330245408	24.90948215454647
48	23.58563874131338	27.194718659730704	22.373711427420027	26.84593117153589
49	20.775535421227257	29.016104418440904	23.458669070365733	26.74969108996611
50	20.54035288770847	32.94312305715318	24.284243798054984	22.23228025708337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21078.0
1	15629.5
2	7905.0
3	5299.5
4	4906.5
5	4343.0
6	3207.5
7	2627.0
8	2744.0
9	2924.5
10	3125.0
11	3584.0
12	4003.5
13	4013.0
14	4244.5
15	4636.0
16	4677.5
17	5263.5
18	6085.5
19	7189.5
20	8476.0
21	9291.0
22	10228.0
23	13677.5
24	16619.5
25	18359.0
26	20941.5
27	24603.5
28	26457.0
29	25350.5
30	27309.5
31	31342.0
32	38433.5
33	46833.0
34	51742.0
35	66985.0
36	77477.0
37	71618.0
38	71740.5
39	89903.5
40	111754.0
41	131756.0
42	153637.5
43	156953.0
44	146773.5
45	141009.5
46	143721.5
47	150512.5
48	167295.0
49	205700.0
50	271936.0
51	300292.0
52	279805.5
53	259313.0
54	229438.5
55	189270.5
56	153321.5
57	127508.0
58	113254.5
59	120030.5
60	126204.5
61	111582.0
62	91988.5
63	82744.5
64	80953.0
65	73421.5
66	59098.0
67	50156.5
68	46232.5
69	44518.5
70	38974.5
71	29909.5
72	24042.5
73	20527.5
74	17175.5
75	13968.0
76	11661.5
77	10085.0
78	9191.5
79	8543.5
80	7309.0
81	6188.5
82	5134.0
83	4561.5
84	4093.5
85	3048.0
86	2260.5
87	1400.5
88	479.0
89	199.0
90	143.0
91	126.0
92	108.0
93	103.5
94	62.0
95	14.5
96	8.5
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	37434.0
25	27360.0
26	34909.0
27	38912.0
28	42681.0
29	30743.0
30	29793.0
31	24479.0
32	25006.0
33	19416.0
34	20957.0
35	20662.0
36	19718.0
37	20267.0
38	25188.0
39	27263.0
40	29993.0
41	27805.0
42	30219.0
43	32686.0
44	34928.0
45	36809.0
46	36865.0
47	65758.0
48	165373.0
49	388413.0
50	1071559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.852833489082787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25276441135078	8.445471522045848
2	12.36895469077494	2.9321432076752965
3	4.863341345371584	1.7293312550178372
4	2.590926515910959	1.22839282302168
5	1.5098304868771701	0.8947884678847946
6	1.0283079500596801	0.7313017744534467
7	0.761995140362063	0.6322261062741259
8	0.5770083918080043	0.5471347511922973
9	0.44393351211983934	0.4735682999442153
>10	3.0800615261556903	7.452816019461918
>50	0.4627859195289431	3.8643835180851625
>100	0.6133607871223838	17.591946022818494
>500	0.36680330957638385	30.959219788421287
>1k	0.07314657438050456	13.962496980155104
>5k	0.00463856325339785	3.7401282491776775
>10k+	0.0021408753477220846	4.814651214370839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	36142	1.5280763201020127	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	23267	0.9837239704447326	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	16271	0.6879345305843575	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	14097	0.5960182581063049	No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	13415	0.5671834384972746	Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTG	10650	0.45027980767767234	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	8652	0.36580477896969216	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT	8189	0.3462292342791041	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	8009	0.3386188713324393	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	7987	0.3376887158611802	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	7547	0.3190856064359994	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	6995	0.29574716006622703	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCACTGCA	6942	0.29350633097637574	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	6415	0.2712248794603069	Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTG	6065	0.2564269515084585	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	5814	0.24581472317727582	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG	5723	0.2419672619097952	No Hit
AGCAGTGGTATCAACGCAGAGTGCAG	5093	0.21533099159646812	No Hit
AAGCAGTGGTATCAACGCAGAGTGC	5004	0.21156808991728382	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCACTGC	4929	0.20839710535617345	No Hit
AAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGC	4434	0.187468607252845	Illumina Single End Adapter 2 (96% over 31bp)
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	4421	0.1869189699289192	No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC	4229	0.17880124945247666	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	3681	0.1556319222592969	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGG	3644	0.1540675698758158	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	3638	0.15381389111092694	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC	3307	0.1398192792478932	Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCA	3302	0.1396078802771525	No Hit
AGCAGTGGTATCAACGCAGAGTGCAGTGC	3262	0.137916688511227	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	3225	0.13635233612774586	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3103	0.131194201241673	No Hit
CATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC	3100	0.13106736185922857	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	3095	0.1308559628884879	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG	3054	0.12912249132841422	No Hit
AGCAGTGGTATCAACGCAGAGTGCAGT	2836	0.11990549620412008	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGACT	2834	0.11982093661582381	No Hit
AAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	2822	0.11931357908604615	Illumina Single End Adapter 2 (100% over 34bp)
AGCAGTGGTATCAACGCAGAGTGCAGTG	2716	0.11483192090634348	No Hit
ACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2636	0.11144953737449244	Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	2630	0.1111958586096036	Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTAGATCGGAAGAGCTCGTATGCCGTCTT	2620	0.1107730606681222	Illumina Single End Adapter 2 (100% over 27bp)
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	2609	0.11030798293249269	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2499	0.10565720557619748	Illumina Single End Adapter 1 (100% over 32bp)
AGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	2474	0.10460021072249404	Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2423	0.10244394122093899	Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATGCCGTCTT	2404	0.10164062513212437	Illumina Single End Adapter 2 (96% over 27bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG	2384	0.10079502924916159	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT	2375	0.10041451110182835	Illumina Single End Adapter 2 (95% over 24bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.17326259641907055	0.0	0.0	0.0	0.0
2	0.5175046803732122	0.0	0.0	0.0	0.0
3	0.8719784745112032	0.0	0.0	0.0	0.0
4	0.9469828293300006	0.0	0.0	0.0	0.0
5	1.3690197345167165	0.0	0.0	0.0	0.0
6	1.5108684438837203	0.0	0.0	0.0	0.0
7	1.7368962233996674	0.0	0.0	0.0	0.0
8	2.0690462862274415	0.0	0.0	0.0	0.0
9	2.186880072518303	0.0	0.0	0.0	0.0
10	2.348050647811006	0.0	0.0	0.0	0.0
11	2.6471801914090842	0.0	0.0	0.0	0.0
12	2.7684386410259445	0.0	0.0	0.0	0.0
13	2.9103296301870967	0.0	0.0	0.0	0.0
14	3.153818964686225	0.0	0.0	0.0	0.0
15	3.422422496909347	0.0	0.0	0.0	0.0
16	3.683880743921434	0.0	0.0	0.0	0.0
17	4.5868925873373705	0.0	0.0	0.0	0.0
18	4.766497152878662	0.0	0.0	0.0	0.0
19	5.234957272040034	0.0	0.0	0.0	0.0
20	5.3553701257739315	0.0	0.0	0.0	0.0
21	5.563217593806179	0.0	0.0	0.0	0.0
22	5.651370964605047	0.0	0.0	0.0	0.0
23	5.812626099486047	0.0	0.0	0.0	0.0
24	6.053536366542138	0.0	0.0	0.0	0.0
25	6.211578237067879	0.0	0.0	0.0	0.0
26	6.577129337272683	0.0	0.0	0.0	0.0
27	6.658517941007849	0.0	0.0	0.0	0.0
28	6.7761826081221175	0.0	0.0	0.0	0.0
29	6.932533286881933	0.0	0.0	0.0	0.0
30	7.006649766023619	0.0	0.0	0.0	0.0
31	7.076961063691973	0.0	0.0	0.0	0.0
32	7.135307179616404	0.0	0.0	0.0	0.0
33	7.19170842501002	0.0	0.0	0.0	0.0
34	7.260582209677337	0.0	0.0	0.0	0.0
35	7.3284412792851	0.0	0.0	0.0	0.0
36	7.38475796509042	0.0	0.0	0.0	0.0
37	7.461918589410772	0.0	0.0	0.0	0.0
38	7.5170514409799445	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	2.6713706E-5	87.17693	44
TCGTAGG	60	0.0	87.17693	44
AAGGTTT	90	0.0	82.33377	44
ACTCGCA	60	0.0	79.91219	44
GTATATG	120	0.0	79.91219	44
GGTCCCT	80	0.0	76.279816	44
TGTTATG	40	1.880835E-9	76.279816	44
CGTGCGC	135	0.0	74.261826	44
CTATCAT	4245	0.0	74.03365	44
ATCCGAG	140	0.0	71.60962	44
ATGTGTG	95	0.0	68.8239	44
GAGCGCT	115	0.0	68.225426	44
CGCTACG	110	0.0	67.36399	44
GTTCGTA	150	0.0	66.83565	44
AATCGCA	125	0.0	66.25447	44
GAAAATC	185	0.0	65.97174	44
GAACACA	2280	0.0	64.42682	44
TAGCGAA	55	0.0	63.672237	43
CACGCTT	100	0.0	63.672237	43
GGGTACG	20	1.267431E-4	63.672234	43
>>END_MODULE