FastQCFastQC Report
Tue 24 May 2016
ERR840895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550345
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC50690.32695948321180124No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC50350.32476642295747077No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT38010.2451712360797113No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT34630.2233696370807788No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG34110.22001554492709685No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30220.19492435554666865No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA26880.17338076363648092No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG26410.17034918034372995No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT23290.1502246274216384No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG22990.1482895742560527No Hit
ATCAAACACAGCAACTTCACATCACTTCACAAGAATTCACAACACTCAA22810.14712854235670125No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG21710.14003334741622026No Hit
TCCCAGATACCCGGTGTCAGCTGGTGAAAGAAGGACGCGATGAAGTTG21530.13887231551686885No Hit
CAGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGAT21300.13738877475658642No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT20670.13332516310885642No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA20190.13022907804391926No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA18670.12042480867161826No Hit
CCAGAGGGATTGGGCTGAGCAGACCCTCTCCTTGGTCTTCATTTTTTA18490.11926377677226682No Hit
ATCTCATTTTGCAAAGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCTG17210.11100754993243439No Hit
AGTCTCCCAGCTCCAGCTCGGCCTTTGGGTTTGCTGTGGTGTCCTTGTC17170.11074954284368962No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC17150.11062053929931726No Hit
ACAACCACGTTAGCAAGCATGCGACTATCTTCAGCACTTCACTCATTGTT16930.10920150031122106No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA16650.10739545069000771No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA16550.1067504329681458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATG800.095.3684444
ACCGACT156.515637E-495.3684444
TGCGATA201.710091E-595.3684444
TATTCGC301.1985321E-895.3684444
CTCGATA450.095.3684444
GTAAGTC1350.095.3684444
ATAGCAC1200.091.3947544
TACTGGA1050.090.8270944
GAACGAT850.089.7585344
GCTTCGA800.089.4079144
TTTGACG550.086.69858644
CTTGCTA409.222276E-1083.4473944
CGATATG409.222276E-1083.4473944
TCGACAT1150.082.9290844
GGGCCCA700.081.7443844
CATGGTT1600.080.46712544
TCCGATC1050.077.20302644
GGGGTAT506.366463E-1176.29475444
TAATGTC255.173449E-576.29475444
GTGCGAA700.074.9323444
Produced by FastQC (version 0.11.5)