##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840895.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1550345
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5581718907727	39.0	37.0	39.0	31.0	39.0
2	37.14018492658086	39.0	37.0	39.0	33.0	39.0
3	37.36490974589527	39.0	37.0	39.0	33.0	39.0
4	37.61575262280331	39.0	37.0	39.0	33.0	41.0
5	38.31511437776753	40.0	37.0	41.0	34.0	41.0
6	38.33024004334519	40.0	38.0	41.0	34.0	41.0
7	38.26215584273178	40.0	37.0	41.0	34.0	41.0
8	38.23564754941642	40.0	37.0	41.0	33.0	41.0
9	38.02469127839287	40.0	37.0	41.0	33.0	41.0
10	37.929998161699494	39.0	37.0	41.0	33.0	41.0
11	37.92481673433978	39.0	37.0	41.0	33.0	41.0
12	38.00084110310931	39.0	37.0	41.0	33.0	41.0
13	38.08820617346461	40.0	37.0	41.0	33.0	41.0
14	38.07050043699951	40.0	37.0	41.0	33.0	41.0
15	38.03567270510757	40.0	37.0	41.0	33.0	41.0
16	38.06067294698922	40.0	37.0	41.0	33.0	41.0
17	38.03607842125462	40.0	37.0	41.0	33.0	41.0
18	37.99037504555438	40.0	36.0	41.0	33.0	41.0
19	37.99066981865327	40.0	36.0	41.0	33.0	41.0
20	37.93945799160832	40.0	36.0	41.0	33.0	41.0
21	37.84192292683242	40.0	36.0	41.0	33.0	41.0
22	37.729673072767675	39.0	36.0	41.0	33.0	41.0
23	37.60677010600866	39.0	35.0	41.0	33.0	41.0
24	37.4621887386356	39.0	35.0	41.0	32.0	41.0
25	37.22740119797898	39.0	35.0	41.0	32.0	41.0
26	37.16775341776376	39.0	35.0	41.0	32.0	41.0
27	37.09539522646791	39.0	35.0	41.0	32.0	41.0
28	37.03584109372301	39.0	35.0	40.0	32.0	41.0
29	36.98437227680665	38.0	35.0	40.0	32.0	41.0
30	36.83640319544532	38.0	35.0	40.0	31.0	41.0
31	36.668353725676255	38.0	35.0	40.0	31.0	41.0
32	36.53890511044841	38.0	35.0	40.0	31.0	41.0
33	36.47234404752013	38.0	35.0	40.0	31.0	41.0
34	36.50341244420849	37.0	35.0	40.0	31.0	41.0
35	36.35816615856151	37.0	35.0	40.0	31.0	41.0
36	36.261536806086106	37.0	34.0	40.0	31.0	41.0
37	36.12782933585216	37.0	34.0	40.0	31.0	41.0
38	36.08969676682349	37.0	34.0	40.0	31.0	41.0
39	36.017109108846164	36.0	34.0	40.0	31.0	41.0
40	35.90563276051275	36.0	34.0	40.0	31.0	41.0
41	35.776250801205585	36.0	34.0	40.0	30.0	41.0
42	35.58501534075957	36.0	34.0	39.0	30.0	41.0
43	35.51975795523142	35.0	34.0	39.0	30.0	41.0
44	35.433268754708855	35.0	34.0	39.0	30.0	41.0
45	35.26574407246304	35.0	34.0	39.0	30.0	41.0
46	35.16892554682632	35.0	34.0	39.0	30.0	41.0
47	35.0611773366281	35.0	33.0	39.0	30.0	40.0
48	35.03351395944299	35.0	33.0	39.0	30.0	40.0
49	35.207177436542814	35.0	33.0	39.0	30.0	40.0
50	35.314417769827806	36.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	50.0
14	297.0
15	1806.0
16	290.0
17	2.0
18	0.0
19	3.0
20	7.0
21	28.0
22	58.0
23	137.0
24	301.0
25	735.0
26	1456.0
27	3229.0
28	6515.0
29	12703.0
30	22801.0
31	38828.0
32	60020.0
33	90858.0
34	127007.0
35	138501.0
36	181573.0
37	216913.0
38	231412.0
39	236619.0
40	178196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.56774137369424	13.053417142635995	37.37884148366976
2	27.749952429943015	22.96733952765352	31.032060605865148	18.25064743653832
3	29.952945957190174	17.16185752203542	24.593364702695204	28.2918318180792
4	20.973783254694922	21.734323650542297	30.137550029187054	27.154343065575727
5	19.94820507693449	26.180946821513924	27.935975540927988	25.9348725606236
6	25.83605584563436	27.67074425369837	25.69924758682745	20.79395231383982
7	29.228784560855807	27.021146906011246	21.341249850839652	22.408818682293298
8	30.896542382501956	24.177070265005533	21.415491390625956	23.510895961866552
9	25.47271736290955	26.110704391603157	24.856918943847983	23.559659301639314
10	28.009636564764616	21.992459742831436	24.39379621955113	25.604107472852817
11	29.214529669202662	24.043745101896675	24.554147625206003	22.18757760369466
12	28.71077082842851	22.201574488259066	26.47597792749356	22.611676755818866
13	26.133602520729255	21.323705368805008	26.791391593484033	25.751300516981708
14	23.47032434716144	26.488491271297683	25.87533742489575	24.165846956645133
15	21.87990415036653	27.507812777156055	23.418722929412485	27.193560143064932
16	22.141910348986837	32.22044125662353	22.886389803559855	22.751258590829785
17	26.52080665916296	29.135708503591136	19.29847872570299	25.045006111542918
18	23.0767345332813	26.283375635745593	20.975460300771765	29.664429530201343
19	24.8376974157365	23.32300229948818	24.113729524718693	27.725570760056634
20	28.715672963114663	21.032350863839984	23.884748233457714	26.36722793958764
21	27.191431584582787	24.52763739683748	22.219570482699012	26.061360535880723
22	26.072325837152377	24.63006621106915	23.130206502423654	26.16740144935482
23	26.840606445662097	24.70508177212169	23.065317719604348	25.388994062611868
24	28.410515078901792	25.089383330807014	22.030515788421287	24.469585801869904
25	27.240807835162673	24.098684201961397	23.912842537757427	24.747665425118505
26	26.80213990871948	24.119895855848515	24.20806230253705	24.86990193289495
27	27.49309188047051	24.521723943803597	23.534707966978626	24.45047620874726
28	25.248787164656783	25.45156562985059	24.92754338558659	24.37210381990604
29	29.073796310689147	23.84553424889827	23.08891127444944	23.991758165963137
30	27.10254543676609	25.189732961382482	23.218723940578407	24.488997661273018
31	25.185560493475045	26.808674893079036	25.40015659344803	22.605608019997888
32	24.88691601521022	25.377920831872007	26.05929550700847	23.675867645909296
33	27.11438947429426	25.12905412906879	25.029972398161927	22.72658399847502
34	27.116345583115002	23.451409371494876	24.858070062658292	24.57417498273183
35	24.82469838882448	25.820319136005622	25.046326538300512	24.308655936869386
36	26.098658890455127	25.84048005911069	24.108623058188876	23.95223799224531
37	25.256494578917177	24.204801613286435	26.80792098141591	23.730782826380477
38	24.525789327893666	25.842316409410472	24.50020866512062	25.131685597575242
39	24.0432896535898	26.495543603619424	24.769801430444577	24.6913653123462
40	25.25301933176392	27.875605467034152	22.32600593517762	24.54536926602431
41	25.132269367383852	26.601377960891398	23.501529523063823	24.764823148660923
42	25.263272381004537	26.471594876171096	23.235888706858393	25.02924403596598
43	25.337165683170515	28.410616830719576	23.9924297488081	22.25978773730181
44	24.96587784032857	29.108892975748702	22.580602390657	23.34462679326573
45	24.124752366932157	23.616683433179304	27.24736670622454	25.011197493664
46	23.94267885051412	26.047930978617007	25.689976053049225	24.319414117819644
47	23.79548652767954	27.59365739143466	24.2600578202697	24.350798260616095
48	25.249287993099014	25.61068284200293	22.337248230252076	26.80278093464598
49	23.35681156184348	26.398392384122765	23.970933005856025	26.27386304817773
50	23.537456429984747	29.7014267074239	25.606917295075437	21.15419956751591
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11807.0
1	8814.5
2	5082.5
3	3663.5
4	2937.5
5	2805.0
6	2378.0
7	2164.5
8	2588.5
9	2978.5
10	3119.0
11	3015.5
12	2968.0
13	3115.0
14	3019.5
15	3132.0
16	3521.0
17	4071.5
18	4779.0
19	5491.0
20	5671.0
21	5362.5
22	6555.5
23	8340.0
24	8345.0
25	10308.0
26	12350.5
27	12585.0
28	12956.0
29	17053.0
30	22284.0
31	22826.5
32	24005.5
33	30713.0
34	39320.5
35	45642.5
36	50457.5
37	51979.0
38	54105.5
39	67013.0
40	78276.0
41	78004.0
42	82996.0
43	89844.0
44	89360.0
45	85190.0
46	83920.5
47	84600.5
48	87880.5
49	104014.5
50	118716.5
51	113822.0
52	106485.5
53	105224.0
54	102603.0
55	98884.5
56	96456.0
57	95819.5
58	95815.5
59	93583.5
60	85446.0
61	80792.0
62	82668.5
63	81904.5
64	73799.5
65	67642.5
66	65406.5
67	58136.0
68	48804.0
69	43036.5
70	38684.0
71	33192.0
72	28594.5
73	25397.0
74	22525.5
75	20040.5
76	15937.0
77	12366.0
78	11024.0
79	10049.0
80	8815.0
81	6141.0
82	3710.5
83	3097.5
84	2886.0
85	2171.0
86	1660.0
87	1359.0
88	880.0
89	560.5
90	426.0
91	319.5
92	230.5
93	178.0
94	89.5
95	3.0
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	14091.0
25	14133.0
26	10494.0
27	12454.0
28	13096.0
29	13481.0
30	14042.0
31	12947.0
32	13456.0
33	11908.0
34	13382.0
35	12866.0
36	13459.0
37	12315.0
38	12978.0
39	12065.0
40	12371.0
41	16009.0
42	16030.0
43	16610.0
44	20715.0
45	20784.0
46	26206.0
47	45925.0
48	183218.0
49	332338.0
50	652972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.46409348438656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52173039419635	7.1701779255680265
2	13.385848518223753	2.801415405250613
3	5.473464697493912	1.7182453883419768
4	2.9333628254532074	1.2277993131666662
5	1.7523588950576083	0.9168423648039575
6	1.1143209777006193	0.6996215329363387
7	0.771703382324255	0.5652623434400814
8	0.6098018770827642	0.5104819078998757
9	0.43888758379790166	0.4133304635398004
>10	3.165571844152298	6.621957814877802
>50	0.48241985564271256	3.5976803216254014
>100	0.7103832612465486	17.995944725838065
>500	0.545879938824321	42.43429221674362
>1k	0.09303371417886283	12.675532366971792
>5k	0.0012322346248856005	0.6514159089959869
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	5069	0.32695948321180124	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	5035	0.32476642295747077	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3801	0.2451712360797113	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3463	0.2233696370807788	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	3411	0.22001554492709685	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3022	0.19492435554666865	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2688	0.17338076363648092	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	2641	0.17034918034372995	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	2329	0.1502246274216384	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	2299	0.1482895742560527	No Hit
ATCAAACACAGCAACTTCACATCACTTCACAAGAATTCACAACACTCAA	2281	0.14712854235670125	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	2171	0.14003334741622026	No Hit
TCCCAGATACCCGGTGTCAGCTGGTGAAAGAAGGACGCGATGAAGTTG	2153	0.13887231551686885	No Hit
CAGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGAT	2130	0.13738877475658642	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	2067	0.13332516310885642	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2019	0.13022907804391926	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	1867	0.12042480867161826	No Hit
CCAGAGGGATTGGGCTGAGCAGACCCTCTCCTTGGTCTTCATTTTTTA	1849	0.11926377677226682	No Hit
ATCTCATTTTGCAAAGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCTG	1721	0.11100754993243439	No Hit
AGTCTCCCAGCTCCAGCTCGGCCTTTGGGTTTGCTGTGGTGTCCTTGTC	1717	0.11074954284368962	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1715	0.11062053929931726	No Hit
ACAACCACGTTAGCAAGCATGCGACTATCTTCAGCACTTCACTCATTGTT	1693	0.10920150031122106	No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA	1665	0.10739545069000771	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	1655	0.1067504329681458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08404580915860663	0.0	0.0	0.0	0.0
2	0.10836297727280057	0.0	0.0	0.0	0.0
3	0.14229090944273695	0.0	0.0	0.0	0.0
4	0.2295618072106531	0.0	0.0	0.0	0.0
5	0.26787585988925045	0.0	0.0	0.0	0.0
6	0.32321838042500217	0.0	0.0	0.0	0.0
7	0.43158135769780276	0.0	0.0	0.0	0.0
8	0.494212578490594	0.0	0.0	0.0	0.0
9	0.5621974463748391	0.0	0.0	0.0	0.0
10	0.6134763552628608	0.0	0.0	0.0	0.0
11	0.6821062408689679	0.0	0.0	0.0	0.0
12	0.7316436019079624	0.0	0.0	0.0	0.0
13	0.7866636135827831	0.0	0.0	0.0	0.0
14	0.8531649407067459	0.0	0.0	0.0	0.0
15	0.9244393989724867	0.0	0.0	0.0	0.0
16	0.988747665842119	0.0	0.0	0.0	0.0
17	1.1145261216051912	0.0	0.0	0.0	0.0
18	1.1920572517729924	0.0	0.0	0.0	0.0
19	1.28997094195163	0.0	0.0	0.0	0.0
20	1.3544082123656347	0.0	0.0	0.0	0.0
21	1.422457582022066	0.0	0.0	0.0	0.0
22	1.4810251911671273	0.0	0.0	0.0	0.0
23	1.5389477825903266	0.0	0.0	0.0	0.0
24	1.6029980423712142	0.0	0.0	0.0	0.0
25	1.6615011497440892	0.0	0.0	0.0	0.0
26	1.735742689530395	0.0	0.0	0.0	0.0
27	1.7910852100661465	0.0	0.0	0.0	0.0
28	1.8504268404774422	0.0	0.0	0.0	0.0
29	1.9213142881100658	0.0	0.0	0.0	0.0
30	1.9813654380154095	0.0	0.0	0.0	0.0
31	2.044770680074435	0.0	0.0	0.0	0.0
32	2.1019192502314	0.0	0.0	0.0	0.0
33	2.1570682654505933	0.0	0.0	0.0	0.0
34	2.2106692381373176	0.0	0.0	0.0	0.0
35	2.27226843057513	0.0	0.0	0.0	0.0
36	2.3342546336460592	0.0	0.0	0.0	0.0
37	2.3863075638003153	0.0	0.0	0.0	0.0
38	2.441585582563881	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATG	80	0.0	95.36844	44
ACCGACT	15	6.515637E-4	95.36844	44
TGCGATA	20	1.710091E-5	95.36844	44
TATTCGC	30	1.1985321E-8	95.36844	44
CTCGATA	45	0.0	95.36844	44
GTAAGTC	135	0.0	95.36844	44
ATAGCAC	120	0.0	91.39475	44
TACTGGA	105	0.0	90.82709	44
GAACGAT	85	0.0	89.75853	44
GCTTCGA	80	0.0	89.40791	44
TTTGACG	55	0.0	86.698586	44
CTTGCTA	40	9.222276E-10	83.44739	44
CGATATG	40	9.222276E-10	83.44739	44
TCGACAT	115	0.0	82.92908	44
GGGCCCA	70	0.0	81.74438	44
CATGGTT	160	0.0	80.467125	44
TCCGATC	105	0.0	77.203026	44
GGGGTAT	50	6.366463E-11	76.294754	44
TAATGTC	25	5.173449E-5	76.294754	44
GTGCGAA	70	0.0	74.93234	44
>>END_MODULE