FastQCFastQC Report
Tue 24 May 2016
ERR840894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15054620
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA4366792.900631168372234No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT900250.5979891887008772No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC636350.42269416298784024No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA587250.3900795901856042No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA580240.38542321227636434No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG511700.3398956599369496No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG460840.3061120107980142No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC435760.2894526730000492No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC420560.2793561046376461No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG393600.2614479807527523No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT378560.25145769205732194No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC358560.238172733685739No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG357910.23774097253866253No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG354560.2355157420114224No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG321240.21338300136436525No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT309400.20551830600838814No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA305380.20284802937569996No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG285840.1898686250466634No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG276770.18384389642515053No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT272960.181313111855364No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG262260.17420565912656713No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA258810.17191400380746907No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG254900.1693167944458246No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT253480.16837356240144222No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT241200.16021659796129029No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC240100.15948592525085323No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG237710.15789837272544907No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT232830.1546568428827828No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC225170.14956870382646656No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG223730.14861218682371258No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT219720.1459485526702102No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC215910.14341776810042364No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT214550.142514390931156No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG211990.14081391625959339No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT209850.13939242571383403No Hit
CTTGTTTCCTGAGTTGTCCTGTGCTGGAGGTCTGCTCAGACGAAGGTCT205480.13648966230964316No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG204320.13571913472409133No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA191720.12734961094999409No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG187550.12457969712951905No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC181880.12081341143117529No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC180140.11965762005284758No Hit
CACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTT177330.11779108340164016No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG177110.11764494885955275No Hit
ACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAAC173910.11551935552009948No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC173680.11536657849882627No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG171190.1137126011815642No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG170830.1134734719308757No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC170590.1133140524304167No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT167780.11144751577920932No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC162600.10800671156096932No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC161080.10699705472472902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA500400.0169.0052244
CTATCAT123050.0135.8643344
CTATGCG5550.0132.3895744
ACATTGG41600.0116.6600644
CGACGGG99000.097.40018544
AATCGCA19400.086.5031144
AGTACTA2250.084.6639544
GAACACA132650.083.4968644
ACTTCTA60700.081.8942944
AATATAC6000.080.1283844
CGTGCGC29500.069.4941344
TTAAGCC488500.065.3348443
ATATTCG2400.064.2538944
ACGCTAT2300.058.95508243
TAATTCC30800.058.3144544
TCGTGCG64950.056.5247143
CAATGAA60550.055.7614843
AACGACA28050.054.86710443
ATCCTTG61800.053.59140443
ACGTATT1150.053.05957443
Produced by FastQC (version 0.11.5)