FastQCFastQC Report
Tue 24 May 2016
ERR840893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6140879
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2646464.309578482168432No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG564070.9185492826027023No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC556880.9068408610558847No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC452690.7371745966660472No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT430250.7006325967341157No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT358330.583515812638549No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC288190.4692976363807201No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA240130.3910352247617971No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG223710.36429638167434986No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC195830.31889571509225306No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG177610.28922569553967764No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA176370.2872064406414782No Hit
CCGATCGCACGCCCCCCGTGGCGGCG169790.2764913622300651No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA165070.2688051661659512No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC158150.25753642108890273No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG155530.25326993090077166No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG151390.24652822503097682No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC149000.2426362740578344No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT147340.2399330779844384No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG132380.215571744696484No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC128220.20879747019929884No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG126580.20612684275329313No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG124330.20246287217188288No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT109920.17899717613716212No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT95090.15484753892724476No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC92660.15089045069932172No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC91700.14932715658458667No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG90740.14776386246985163No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG89750.14615171541403113No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA87280.14212948993132743No Hit
AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT85950.13996367620987157No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTGCA85470.13918202915250408No Hit
CTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACCCTGCGT84180.13708135268582886No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT81820.13323825465377187No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT79230.12902061740672632No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG76260.12418417623926478No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC75750.12335367624081178No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC75610.12312569584907959No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC75360.12271858800670067No Hit
CCGATCGCACGCCCCCCGTGGCGGCGAC74740.12170896055760094No Hit
AAGCAGTGGTATCAACGCAGAGTG74600.12148098016586877No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73690.11999910761960951No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC73550.1197711272278773No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG72180.11754017625164084No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGA69110.11254089194722774No Hit
ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC68640.1117755292035554No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG68410.11140098998856679No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA67880.11053792136272347No Hit
CCGATCGCACGCCCCCCGTGGCGG67080.10923517626711095No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG63240.1029819998081708No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG62550.10185838216320497No Hit
CCTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGC61880.10076733314562948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA302650.0123.8138544
ACGTTCT1750.0113.4949844
CTATCAT55050.098.13554444
CGTGATT1850.093.0454444
CGATAAA504.0199666E-1092.6875844
TAAAACG1600.091.0324444
CGACGGG86400.089.11677644
CGACATA1600.078.6189244
ACTTCTA28950.077.9828944
GAACACA41550.077.2794844
CGACTAG801.8189894E-1274.4810944
AATCGCA5700.070.8514144
TCGTAGC950.069.689944
GATACTT1550.068.3410744
CACGCTA301.4814577E-766.37228443
TAAACGT402.1827873E-1066.37228443
GAATCAT4150.063.81244344
TTAAGCC304300.063.547743
CCATCGA1600.062.0675744
TAGGATA1200.060.8412643
Produced by FastQC (version 0.11.5)