##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840893.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6140879 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.83661329265729 39.0 37.0 39.0 32.0 39.0 2 37.27692110526848 39.0 37.0 39.0 34.0 39.0 3 37.49466582878445 39.0 37.0 39.0 34.0 39.0 4 37.92437483298401 39.0 37.0 40.0 34.0 41.0 5 38.39016547956734 40.0 38.0 41.0 34.0 41.0 6 38.35351437473365 40.0 38.0 41.0 34.0 41.0 7 38.107985355191005 40.0 37.0 41.0 33.0 41.0 8 38.02475427377742 39.0 37.0 41.0 33.0 41.0 9 37.92161480465582 39.0 37.0 41.0 33.0 41.0 10 37.849350231457095 39.0 36.0 41.0 33.0 41.0 11 37.94284319883196 39.0 37.0 41.0 33.0 41.0 12 38.119570341640014 40.0 37.0 41.0 33.0 41.0 13 38.22229472360553 40.0 37.0 41.0 34.0 41.0 14 38.221792352528034 40.0 37.0 41.0 34.0 41.0 15 38.201010799919686 40.0 37.0 41.0 34.0 41.0 16 38.20679710510498 40.0 37.0 41.0 34.0 41.0 17 38.208549785787994 40.0 37.0 41.0 34.0 41.0 18 38.19960806913799 40.0 37.0 41.0 34.0 41.0 19 38.01363648428833 40.0 37.0 41.0 33.0 41.0 20 38.05197155000123 40.0 37.0 41.0 33.0 41.0 21 37.87827231248165 40.0 36.0 41.0 33.0 41.0 22 37.820253908275994 40.0 36.0 41.0 33.0 41.0 23 37.64969477496626 39.0 36.0 41.0 33.0 41.0 24 37.54057098340482 39.0 35.0 41.0 32.0 41.0 25 37.41168712293065 39.0 35.0 41.0 32.0 41.0 26 37.32962094376954 39.0 35.0 41.0 32.0 41.0 27 37.20948937366785 39.0 35.0 41.0 31.0 41.0 28 37.2435046946099 39.0 35.0 41.0 31.0 41.0 29 37.11789786002508 39.0 35.0 41.0 31.0 41.0 30 37.0636849229306 39.0 35.0 41.0 31.0 41.0 31 36.95084762852215 39.0 35.0 41.0 31.0 41.0 32 37.0477800264645 39.0 35.0 41.0 31.0 41.0 33 37.00790349084633 39.0 35.0 40.0 31.0 41.0 34 36.87059983982455 38.0 35.0 40.0 31.0 41.0 35 36.777575419726936 38.0 35.0 40.0 31.0 41.0 36 36.75039452323124 38.0 35.0 40.0 31.0 41.0 37 36.702249135809616 38.0 35.0 40.0 31.0 41.0 38 36.49634258753298 38.0 35.0 40.0 31.0 41.0 39 36.39396804125514 38.0 34.0 40.0 31.0 41.0 40 36.28563025138305 37.0 34.0 40.0 30.0 41.0 41 36.20413911116625 37.0 34.0 40.0 30.0 41.0 42 36.08902101576555 37.0 34.0 40.0 30.0 41.0 43 36.00586171046704 37.0 34.0 40.0 30.0 41.0 44 35.877954596228165 37.0 34.0 40.0 30.0 41.0 45 35.62097996777108 36.0 34.0 40.0 29.0 41.0 46 35.53870358445843 36.0 33.0 40.0 29.0 41.0 47 35.44489281864211 36.0 33.0 39.0 29.0 41.0 48 35.428574628955474 36.0 33.0 39.0 29.0 41.0 49 35.66651601563321 36.0 34.0 40.0 29.0 41.0 50 35.92300726092969 37.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 8.0 14 329.0 15 4515.0 16 6263.0 17 199.0 18 3.0 19 8.0 20 38.0 21 184.0 22 377.0 23 870.0 24 1664.0 25 3435.0 26 6612.0 27 12780.0 28 25100.0 29 48214.0 30 87185.0 31 147261.0 32 230533.0 33 338172.0 34 464055.0 35 488850.0 36 614485.0 37 786381.0 38 927507.0 39 1040184.0 40 905667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 38.052239752647786 19.451922110824853 42.49583813652736 2 23.152255564716388 23.766434740042914 31.117662471447495 21.963647223793206 3 25.376904511552823 13.98623877786877 26.293076284356033 34.34378042622237 4 16.589058341647835 17.84781950596975 33.23726131063648 32.32586084174594 5 20.28305719751195 19.957256933412953 35.19108583640877 24.568600032666332 6 27.192312370916284 22.710592408676348 24.288102729267262 25.808992491140113 7 30.39734213945593 19.902574208024618 22.8519728201777 26.848110832341753 8 26.075045608291582 17.154433428829975 29.43057500400187 27.339945958876573 9 23.728085181290822 24.336336866432315 29.087936759542078 22.847641192734784 10 30.241712953471318 15.128143707114242 23.580142191370324 31.050001148044114 11 27.69080778175242 24.37484926832136 22.809421908492254 25.12492104143397 12 23.81561336740229 18.792553964994262 27.00154163597752 30.390291031625928 13 23.405476642676074 19.374148228616782 23.800957485076648 33.419417643630496 14 23.05399601587981 23.739419063622652 20.433833658015406 32.77275126248213 15 22.808379712415764 23.235696388090368 26.861757087218297 27.09416681227557 16 28.634597750582614 23.14235470198973 18.946359307845018 29.276688239582636 17 25.84809112832218 21.418236705201323 21.037069774538793 31.696602391937702 18 25.756605853982794 22.560043928564625 17.454585898859104 34.22876431859348 19 23.219607486159553 28.787458603239045 22.557324448177532 25.435609462423862 20 35.73452595304353 16.846806458814772 20.521329275499482 26.897338312642212 21 29.53689528811755 23.15712457451124 22.332389223106333 24.97359091426488 22 26.780074969723387 25.386951933102736 21.858287714185543 25.974685382988334 23 33.280235614477995 21.151841617462257 20.557089628374047 25.0108331396857 24 31.015836657911677 22.10895215489509 19.354769895319546 27.520441291873688 25 27.67238008031209 24.878938188501536 22.383089795417625 25.065591935768744 26 28.451172781885113 21.775590098576618 26.255689393495764 23.517547726042505 27 26.378976989031404 26.867543984451757 19.972004708497305 26.781474318019534 28 25.356955622355855 19.865968921154124 27.251800695487727 27.525274761002294 29 35.86596734322478 21.73150676677933 20.946291288685224 21.456234601310666 30 27.67427340970432 25.697611184658108 19.07584111567331 27.552274289964256 31 23.204539604547783 21.890122571629426 29.49781856644858 25.40751925737421 32 24.6409460335177 19.73570072186489 26.745723859781194 28.87762938483622 33 31.482692178715986 19.304961745624187 23.31406160300307 25.89828447265675 34 24.685246635741514 22.685027040371104 27.94986192761677 24.67986439627061 35 22.68892556930407 22.16737063362097 23.30300860299415 31.84069519408081 36 23.33289934254052 20.852203094525024 32.028849659859716 23.78604790307474 37 24.55790873212671 20.479859656551756 23.49459907523376 31.467632536087777 38 25.319936319002256 26.316799745007906 22.725983854100438 25.637280081889397 39 24.09259313935026 27.655667288445056 21.17707369738242 27.074665874822262 40 23.908333973962918 29.741536808677637 20.72200377544367 25.628125441915778 41 28.450699039957385 21.75337257465416 21.377807938170086 28.418120447218364 42 25.624363405852048 26.0361995104217 23.132119710591397 25.207317373134856 43 25.710370548042867 18.97624818758258 28.251432233876912 27.061949030497644 44 26.5988359981096 21.387274864995383 26.174606632517218 25.839282504377802 45 27.000811662978847 25.11042824836847 26.91070519236775 20.978054896284938 46 25.814949030144994 28.621502495923313 20.7182703550094 24.845278118922298 47 30.442427457220877 20.374469088305332 21.896468196294265 27.28663525817952 48 24.15260184163276 20.430834445231465 20.672256357037355 34.74430735609842 49 25.166268613757158 19.629829659226825 21.07589092972978 34.12801079728624 50 22.5491550694228 37.28432232572537 18.368952483165117 21.797570121686714 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26640.0 1 19615.5 2 10645.0 3 7019.0 4 4964.5 5 4675.0 6 4017.0 7 3380.0 8 3685.5 9 3668.5 10 3397.0 11 4069.5 12 5238.0 13 6052.0 14 6765.5 15 7598.0 16 8079.0 17 8917.0 18 9669.0 19 9054.0 20 8555.0 21 10454.5 22 12421.5 23 16224.0 24 19848.0 25 21627.5 26 24281.0 27 26490.0 28 26321.5 29 27671.0 30 34928.5 31 48849.0 32 66074.0 33 76724.5 34 88167.5 35 107095.0 36 111423.5 37 103224.5 38 106989.0 39 131761.5 40 162586.5 41 196782.0 42 239491.5 43 388982.0 44 515023.0 45 420974.0 46 339546.0 47 314168.5 48 297193.0 49 359049.0 50 453177.5 51 490417.0 52 482222.0 53 507105.5 54 521547.0 55 478840.0 56 436060.5 57 423112.0 58 407964.0 59 396669.5 60 389393.0 61 378267.0 62 346643.5 63 310928.0 64 328369.5 65 345819.5 66 283555.5 67 224732.0 68 199406.0 69 177733.0 70 159960.0 71 165074.5 72 188589.5 73 169890.0 74 131389.5 75 117805.5 76 109953.5 77 80015.0 78 58249.0 79 61945.5 80 69374.5 81 61963.0 82 48164.5 83 47700.5 84 44600.5 85 34011.5 86 26450.0 87 13744.5 88 3435.5 89 2148.0 90 1315.5 91 598.0 92 336.0 93 256.5 94 257.0 95 376.0 96 467.5 97 466.5 98 234.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 60101.0 25 52675.0 26 67815.0 27 54792.0 28 63278.0 29 55291.0 30 64314.0 31 53131.0 32 48295.0 33 47299.0 34 52322.0 35 52940.0 36 46499.0 37 50980.0 38 53604.0 39 49288.0 40 53174.0 41 51862.0 42 64366.0 43 58655.0 44 73851.0 45 77575.0 46 104078.0 47 231669.0 48 842463.0 49 1843308.0 50 1867254.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.02278009079803 #Duplication Level Percentage of deduplicated Percentage of total 1 67.04277175806234 5.3786941449249905 2 13.186530902152677 2.11585275176967 3 5.501835541159003 1.3242004992736638 4 2.9706720000376774 0.9533219271277377 5 1.7984932395776563 0.7214457877959237 6 1.2724505294164936 0.6125154464356835 7 0.8875263528024178 0.49843001273252785 8 0.6861166590180762 0.44036504575480656 9 0.5433184261307541 0.39230318269129805 >10 4.03461205459827 6.541798531382188 >50 0.6112364547890375 3.4309884920731704 >100 0.8532252429385382 17.14897832927172 >500 0.4927355772228691 28.03443317998438 >1k 0.10470377863716536 14.424916516310905 >5k 0.008910959884014073 4.937600717698356 >10k+ 0.004860523573098585 13.044155434772975 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 264646 4.309578482168432 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 56407 0.9185492826027023 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC 55688 0.9068408610558847 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC 45269 0.7371745966660472 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT 43025 0.7006325967341157 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 35833 0.583515812638549 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 28819 0.4692976363807201 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 24013 0.3910352247617971 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG 22371 0.36429638167434986 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC 19583 0.31889571509225306 No Hit CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG 17761 0.28922569553967764 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 17637 0.2872064406414782 No Hit CCGATCGCACGCCCCCCGTGGCGGCG 16979 0.2764913622300651 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 16507 0.2688051661659512 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC 15815 0.25753642108890273 No Hit ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG 15553 0.25326993090077166 No Hit CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG 15139 0.24652822503097682 No Hit CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC 14900 0.2426362740578344 No Hit CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT 14734 0.2399330779844384 No Hit CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG 13238 0.215571744696484 No Hit TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC 12822 0.20879747019929884 No Hit CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG 12658 0.20612684275329313 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG 12433 0.20246287217188288 No Hit ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCCTGGTTCTTCCTGAGGTCT 10992 0.17899717613716212 No Hit ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT 9509 0.15484753892724476 No Hit CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC 9266 0.15089045069932172 No Hit ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC 9170 0.14932715658458667 No Hit CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG 9074 0.14776386246985163 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG 8975 0.14615171541403113 No Hit CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA 8728 0.14212948993132743 No Hit AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 8595 0.13996367620987157 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCACTGCA 8547 0.13918202915250408 No Hit CTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACCCTGCGT 8418 0.13708135268582886 No Hit ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT 8182 0.13323825465377187 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 7923 0.12902061740672632 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 7626 0.12418417623926478 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 7575 0.12335367624081178 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC 7561 0.12312569584907959 No Hit CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC 7536 0.12271858800670067 No Hit CCGATCGCACGCCCCCCGTGGCGGCGAC 7474 0.12170896055760094 No Hit AAGCAGTGGTATCAACGCAGAGTG 7460 0.12148098016586877 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7369 0.11999910761960951 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 7355 0.1197711272278773 No Hit AAGCAGTGGTATCAACGCAGAGTGCAG 7218 0.11754017625164084 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGA 6911 0.11254089194722774 No Hit ACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 6864 0.1117755292035554 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG 6841 0.11140098998856679 No Hit CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA 6788 0.11053792136272347 No Hit CCGATCGCACGCCCCCCGTGGCGG 6708 0.10923517626711095 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG 6324 0.1029819998081708 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 6255 0.10185838216320497 No Hit CCTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGACTTCTCCGC 6188 0.10076733314562948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.08969399983292294 0.0 0.0 0.0 0.0 2 0.1276690193700283 0.0 0.0 0.0 0.0 3 0.14216205855871775 0.0 0.0 0.0 0.0 4 0.17289055850147836 0.0 0.0 0.0 0.0 5 0.2283060780060965 0.0 0.0 0.0 0.0 6 0.2916357739665608 0.0 0.0 0.0 0.0 7 0.3372969895677801 0.0 0.0 0.0 0.0 8 0.40222254827036974 0.0 0.0 0.0 0.0 9 0.44619019524729275 0.0 0.0 0.0 0.0 10 0.49048352849811894 0.0 0.0 0.0 0.0 11 0.5674757636488197 0.0 0.0 0.0 0.0 12 0.6084796655332242 0.0 0.0 0.0 0.0 13 0.659807822300358 0.0 0.0 0.0 0.0 14 0.7270294692339647 0.0 0.0 0.0 0.0 15 0.7998040671376199 0.0 0.0 0.0 0.0 16 0.8575645278143406 0.0 0.0 0.0 0.0 17 1.0348029980724258 0.0 0.0 0.0 0.0 18 1.0902022332633488 0.0 0.0 0.0 0.0 19 1.207563282064343 0.0 0.0 0.0 0.0 20 1.260585007455773 0.0 0.0 0.0 0.0 21 1.338749713192525 0.0 0.0 0.0 0.0 22 1.3947840366175592 0.0 0.0 0.0 0.0 23 1.4512906051397527 0.0 0.0 0.0 0.0 24 1.524179193239274 0.0 0.0 0.0 0.0 25 1.5864992617506386 0.0 0.0 0.0 0.0 26 1.6887484674425273 0.0 0.0 0.0 0.0 27 1.7461018202768692 0.0 0.0 0.0 0.0 28 1.812509251525718 0.0 0.0 0.0 0.0 29 1.8914393200061423 0.0 0.0 0.0 0.0 30 1.9492323493102535 0.0 0.0 0.0 0.0 31 2.001944672741476 0.0 0.0 0.0 0.0 32 2.0521166432362534 0.0 0.0 0.0 0.0 33 2.1053174960783303 0.0 0.0 0.0 0.0 34 2.164136437145236 0.0 0.0 0.0 0.0 35 2.226489074283991 0.0 0.0 0.0 0.0 36 2.2906818388702987 0.0 0.0 0.0 0.0 37 2.3512920544436717 0.0 0.0 0.0 0.0 38 2.4009429269002043 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGCCA 30265 0.0 123.81385 44 ACGTTCT 175 0.0 113.49498 44 CTATCAT 5505 0.0 98.135544 44 CGTGATT 185 0.0 93.04544 44 CGATAAA 50 4.0199666E-10 92.68758 44 TAAAACG 160 0.0 91.03244 44 CGACGGG 8640 0.0 89.116776 44 CGACATA 160 0.0 78.61892 44 ACTTCTA 2895 0.0 77.98289 44 GAACACA 4155 0.0 77.27948 44 CGACTAG 80 1.8189894E-12 74.48109 44 AATCGCA 570 0.0 70.85141 44 TCGTAGC 95 0.0 69.6899 44 GATACTT 155 0.0 68.34107 44 CACGCTA 30 1.4814577E-7 66.372284 43 TAAACGT 40 2.1827873E-10 66.372284 43 GAATCAT 415 0.0 63.812443 44 TTAAGCC 30430 0.0 63.5477 43 CCATCGA 160 0.0 62.06757 44 TAGGATA 120 0.0 60.84126 43 >>END_MODULE