##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840892.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9499449 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.157192380315955 39.0 37.0 39.0 33.0 39.0 2 37.48890856722321 39.0 37.0 39.0 35.0 39.0 3 37.51737779738593 39.0 37.0 39.0 35.0 39.0 4 37.692484058812255 39.0 37.0 39.0 34.0 40.0 5 38.57291407112139 40.0 38.0 41.0 34.0 41.0 6 38.533438834189226 40.0 38.0 41.0 34.0 41.0 7 38.56662802232003 40.0 38.0 41.0 34.0 41.0 8 38.48924469198161 40.0 38.0 41.0 34.0 41.0 9 38.39693028511443 40.0 38.0 41.0 34.0 41.0 10 38.372693405691216 40.0 38.0 41.0 34.0 41.0 11 38.31101698635363 40.0 38.0 41.0 34.0 41.0 12 38.438946195721456 40.0 38.0 41.0 34.0 41.0 13 38.58009417177775 40.0 38.0 41.0 34.0 41.0 14 38.60231809234409 40.0 38.0 41.0 34.0 41.0 15 38.594663648386344 40.0 38.0 41.0 34.0 41.0 16 38.60777704054203 40.0 38.0 41.0 34.0 41.0 17 38.58735259276617 40.0 38.0 41.0 34.0 41.0 18 38.599876477046195 40.0 38.0 41.0 34.0 41.0 19 38.559975320673864 40.0 38.0 41.0 34.0 41.0 20 38.57924117493552 40.0 38.0 41.0 34.0 41.0 21 38.48979535549904 40.0 38.0 41.0 34.0 41.0 22 38.44348688013378 40.0 38.0 41.0 34.0 41.0 23 38.37676479972681 40.0 38.0 41.0 34.0 41.0 24 38.3075229942284 40.0 37.0 41.0 34.0 41.0 25 38.189434625680065 40.0 37.0 41.0 33.0 41.0 26 38.11018455814874 40.0 37.0 41.0 33.0 41.0 27 38.095581771609965 40.0 37.0 41.0 33.0 41.0 28 38.10082306946594 40.0 37.0 41.0 33.0 41.0 29 38.063650803282144 40.0 37.0 41.0 33.0 41.0 30 38.04322602761193 40.0 37.0 41.0 33.0 41.0 31 37.939397631243814 40.0 37.0 41.0 33.0 41.0 32 37.85649841460204 40.0 36.0 41.0 33.0 41.0 33 37.85095761346468 40.0 36.0 41.0 33.0 41.0 34 37.80660445854312 40.0 36.0 41.0 33.0 41.0 35 37.77237790692617 40.0 36.0 41.0 33.0 41.0 36 37.75279043460241 40.0 36.0 41.0 33.0 41.0 37 37.68361499702133 39.0 36.0 41.0 33.0 41.0 38 37.610728734479316 39.0 36.0 41.0 33.0 41.0 39 37.530584217268455 39.0 35.0 41.0 32.0 41.0 40 37.48510411964721 39.0 35.0 41.0 32.0 41.0 41 37.435366279942706 39.0 35.0 41.0 32.0 41.0 42 37.29957905486469 39.0 35.0 41.0 32.0 41.0 43 37.243292247050384 39.0 35.0 41.0 32.0 41.0 44 37.16738299725547 39.0 35.0 40.0 32.0 41.0 45 37.049551419300805 39.0 35.0 40.0 31.0 41.0 46 36.940703117525494 38.0 35.0 40.0 31.0 41.0 47 36.821374374910626 38.0 35.0 40.0 31.0 41.0 48 36.746540677268456 38.0 35.0 40.0 31.0 41.0 49 36.81716283770306 38.0 35.0 40.0 31.0 41.0 50 36.56567914727593 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 11.0 14 452.0 15 7659.0 16 14610.0 17 544.0 18 3.0 19 8.0 20 42.0 21 121.0 22 283.0 23 633.0 24 1212.0 25 2370.0 26 4603.0 27 9698.0 28 19938.0 29 40800.0 30 79012.0 31 141069.0 32 233597.0 33 365480.0 34 521428.0 35 598744.0 36 818215.0 37 1069646.0 38 1365081.0 39 2014532.0 40 2189658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 50.349846606892676 13.491056165468123 36.1590972276392 2 24.612174874563777 27.46456136561184 29.255812626606026 18.667451133218357 3 26.874874532196554 18.571708738054177 27.237611360406273 27.315805369343 4 22.27335501248546 21.42797966492583 31.09603514898601 25.202630173602703 5 20.41354188016589 24.822134420638502 29.66242568384756 25.101898015348045 6 21.24697969324326 30.51087489390174 27.04451595034617 21.19762946250883 7 27.113541006431003 28.47766223072517 23.28790859343526 21.120888169408563 8 24.57768866383724 26.05095306054067 25.94750495528741 23.423853320334683 9 22.278218452459715 26.86329491321023 28.386193767659577 22.472292866670475 10 23.751567064573955 25.405768271401847 26.14074774231642 24.701916921707774 11 24.040920689189445 27.264455022601837 26.6883268703269 22.006297417881814 12 25.366218609100383 23.91151318355412 26.905107864677202 23.817160342668295 13 21.932440502601782 23.26968648392133 28.8697797103811 25.92809330309579 14 20.95167835523934 25.455202717547092 28.102766802579815 25.490352124633752 15 20.316567834618617 27.52282790296574 28.047963623995454 24.112640638420185 16 24.032499148108485 32.59391149949855 21.903280916608953 21.470308435784013 17 23.702458953145598 30.809292202105613 21.886132553582843 23.602116291165938 18 23.418600384085437 26.47165114524011 23.661688167387393 26.448060303287065 19 21.86548925100814 27.27938220416784 25.956305465717012 24.89882307910701 20 25.020745940106632 24.443838795281707 26.13914764951104 24.396267615100623 21 24.094660648212333 26.543444782955305 25.324858315466507 24.037036253365855 22 23.962179280082456 28.32570604884557 25.152753596550703 22.55936107452127 23 24.68762135572284 27.156543500575665 24.389825136173688 23.766010007527804 24 24.376750693645494 27.068970000260016 25.116740981503245 23.437538324591248 25 25.280334612751652 27.953940940467838 25.026323095867358 21.73940135091315 26 24.72957868647205 27.315420528863704 26.0647232864468 21.890277498217447 27 24.054532034346778 27.915535481188574 24.630443058566062 23.399489425898587 28 24.097285872196306 26.88105926024629 25.407378116181352 23.614276751376053 29 25.372745991079 26.976021316797215 25.593931208217057 22.057301483906734 30 25.78694314492011 26.241732675489292 24.84152258445822 23.12980159513237 31 23.06537687026599 28.384698460464612 25.809434253128412 22.740490416140986 32 23.417850596278683 27.79243677157297 26.400246547301997 22.389466084846347 33 23.79497471748814 26.706628087839306 26.203932587401674 23.294464607270882 34 23.30776839204584 28.403725574734253 25.78469564350867 22.50381038971124 35 23.66548852877531 26.838981431758974 25.814642073814937 23.680887965650783 36 23.38834474210445 27.046532909601723 26.74736401770719 22.817758330586628 37 23.123760771562214 27.95710805459412 25.334789903227257 23.584341270616406 38 22.68218015963933 28.987672957599752 25.39000955473813 22.940137328022786 39 22.84342906580343 29.447015699654262 24.860678287883218 22.84887694665909 40 22.550969381749887 29.337940333174444 24.711436558865024 23.39965372621064 41 23.882591199042277 27.57901418506416 25.72082051383586 22.817574102057694 42 23.288358166693133 28.352182884573423 25.869911906888234 22.48954704184521 43 22.566035033378437 27.430569818438016 25.873508155720774 24.129886992462772 44 23.280667583524696 28.141663026390574 25.92724298824805 22.650426401836683 45 23.705873964187056 28.167275735378798 25.835339739785457 22.29151056064869 46 23.386001795493222 28.53588730925363 25.224820834859734 22.853290060393412 47 24.666703745269498 28.43247528173356 24.530458712117838 22.370362260879105 48 22.542546432379947 27.998650848382596 25.376407771946567 24.08239494729089 49 23.312019264328026 28.331065908534725 23.707988609811554 24.6489262173257 50 22.826220913032266 31.047053166145805 25.231040459654093 20.89568546116784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 85738.0 1 70159.0 2 49803.0 3 38983.0 4 31637.0 5 28097.5 6 23999.0 7 21313.5 8 20382.0 9 20346.5 10 20602.0 11 22365.0 12 24567.0 13 27325.5 14 30598.0 15 32267.5 16 33045.5 17 38672.5 18 44897.5 19 55026.5 20 63470.5 21 69332.0 22 76092.0 23 87428.5 24 98458.5 25 108988.5 26 121982.0 27 142355.5 28 162126.0 29 191541.0 30 221396.5 31 236855.5 32 258285.5 33 307989.0 34 356177.5 35 377669.5 36 399357.5 37 418758.5 38 434185.5 39 464493.5 40 502133.0 41 544659.5 42 589495.0 43 612323.0 44 625673.0 45 600748.0 46 575805.0 47 565822.5 48 560341.5 49 577425.0 50 613961.5 51 642401.0 52 635622.0 53 593693.0 54 559967.0 55 543597.0 56 527691.0 57 504364.0 58 463444.0 59 428404.0 60 401129.0 61 366429.5 62 334617.5 63 310707.0 64 289011.5 65 260167.5 66 229413.5 67 218244.5 68 196971.0 69 167179.5 70 145946.0 71 132648.5 72 122326.5 73 106789.0 74 92941.5 75 84675.5 76 75415.0 77 61318.5 78 45404.5 79 36946.0 80 32587.5 81 24438.0 82 16845.0 83 14412.0 84 12037.0 85 9803.5 86 7575.0 87 4927.0 88 2999.5 89 2077.5 90 1391.0 91 762.0 92 396.0 93 276.0 94 154.5 95 37.5 96 32.0 97 28.0 98 20.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 62869.0 25 56772.0 26 57361.0 27 54092.0 28 55714.0 29 55658.0 30 62421.0 31 67491.0 32 58421.0 33 57488.0 34 60189.0 35 58615.0 36 58597.0 37 63717.0 38 61192.0 39 61858.0 40 61872.0 41 65735.0 42 69236.0 43 76969.0 44 110994.0 45 93096.0 46 104031.0 47 188322.0 48 527693.0 49 1455472.0 50 5793574.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.717079368585331 #Duplication Level Percentage of deduplicated Percentage of total 1 68.22419616749109 6.629399290174459 2 13.898553878424705 2.7010670229042466 3 5.701957735017073 1.6621912760243982 4 2.9282032571271466 1.1381433382741826 5 1.660764064564218 0.8068888113932441 6 1.0839540170880981 0.6319720329565169 7 0.7813423482775476 0.5314655928574841 8 0.5806289181069989 0.45136138247532326 9 0.42498676525249346 0.3716667115701139 >10 2.8708420907698007 5.576936096837805 >50 0.4717423151920814 3.2320218297180667 >100 0.6797347139047646 16.350551996421146 >500 0.5779395454349305 41.605548501017566 >1k 0.11190124031681796 14.3504391277748 >5k 0.002060197253894904 1.2854791076776844 >10k+ 0.001192745778570734 2.674867881923 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 80655 0.8490492448562016 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 26775 0.281858453053435 No Hit CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT 25494 0.26837346039754517 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20182 0.21245442761995986 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 19754 0.20794890314164535 No Hit CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA 18944 0.19942209279717169 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 14664 0.15436684801402692 No Hit CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA 14007 0.14745065740128718 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 12138 0.12777583205089055 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 10960 0.11537511280917451 No Hit CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA 10297 0.10839576063832757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.026685758300297208 0.0 0.0 0.0 0.0 2 0.057466490951212014 0.0 0.0 0.0 0.0 3 0.08751033875754267 0.0 0.0 0.0 0.0 4 0.11469086259634638 0.0 0.0 0.0 0.0 5 0.14276617517500226 0.0 0.0 0.0 0.0 6 0.19660087653504957 0.0 0.0 0.0 0.0 7 0.24886706586876775 0.0 0.0 0.0 0.0 8 0.309733754031418 0.0 0.0 0.0 0.0 9 0.3695372226325969 0.0 0.0 0.0 0.0 10 0.4350778660951809 0.0 0.0 0.0 0.0 11 0.5055977457218834 0.0 0.0 0.0 0.0 12 0.5724963626837725 0.0 0.0 0.0 0.0 13 0.6515219988022464 0.0 0.0 0.0 0.0 14 0.7330109356869015 0.0 0.0 0.0 8.421541080961643E-5 15 0.8205107475180929 0.0 0.0 0.0 8.421541080961643E-5 16 0.9065367896601161 0.0 0.0 0.0 8.421541080961643E-5 17 1.007079463240447 0.0 0.0 0.0 8.421541080961643E-5 18 1.0880841615129466 0.0 0.0 0.0 8.421541080961643E-5 19 1.1870267422878948 0.0 0.0 0.0 8.421541080961643E-5 20 1.2840850032459776 0.0 0.0 0.0 8.421541080961643E-5 21 1.3711847918758235 0.0 0.0 0.0 8.421541080961643E-5 22 1.45513702952666 0.0 0.0 0.0 8.421541080961643E-5 23 1.529425548787093 0.0 0.0 0.0 8.421541080961643E-5 24 1.611914543675112 0.0 0.0 0.0 8.421541080961643E-5 25 1.6947614540590723 0.0 0.0 0.0 8.421541080961643E-5 26 1.7743976519059157 0.0 0.0 0.0 8.421541080961643E-5 27 1.8470755514346149 0.0 0.0 0.0 8.421541080961643E-5 28 1.9199850433430403 0.0 0.0 0.0 8.421541080961643E-5 29 2.0122324989586238 0.0 0.0 0.0 8.421541080961643E-5 30 2.091637104425741 0.0 0.0 0.0 8.421541080961643E-5 31 2.1744629609569985 0.0 0.0 0.0 8.421541080961643E-5 32 2.250846338561321 0.0 0.0 0.0 8.421541080961643E-5 33 2.3265875736582196 0.0 0.0 0.0 8.421541080961643E-5 34 2.4148874318921023 0.0 0.0 0.0 8.421541080961643E-5 35 2.4989870465118558 0.0 0.0 0.0 8.421541080961643E-5 36 2.583507738185657 0.0 0.0 0.0 8.421541080961643E-5 37 2.6630070859899346 0.0 0.0 0.0 8.421541080961643E-5 38 2.7456645116995735 0.0 0.0 0.0 8.421541080961643E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATGCGC 30 1.0014455E-5 56.610306 44 TAAGCCA 10520 0.0 55.727795 44 CGTTCGA 200 0.0 50.156254 43 CGATACG 50 7.2759576E-12 49.339664 41 CCGGTAT 100 0.0 47.552658 44 CGGTCTA 65 0.0 47.07718 37 TACGGAA 100 0.0 46.87268 41 TTAAGCC 10350 0.0 45.473957 43 ATTAAGC 10285 0.0 42.872334 42 GTCCGTT 120 0.0 41.864994 33 ATCCGTT 150 0.0 41.718227 19 CACGATA 160 0.0 41.718224 19 CTAGACG 145 0.0 41.452095 5 ATTCGCG 105 0.0 41.452095 3 CGAATGT 110 0.0 41.45209 11 CGATACT 30 3.8126636E-6 41.45209 4 TCGCGTA 130 0.0 41.329193 24 CGAACAC 170 0.0 40.874817 35 ACGATAT 75 0.0 40.69749 26 CGACTAG 70 0.0 40.351868 37 >>END_MODULE