##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840891.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6492440
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.91139402135407	39.0	37.0	39.0	32.0	39.0
2	37.45935349421789	39.0	37.0	39.0	35.0	39.0
3	37.465609539710805	39.0	37.0	39.0	34.0	39.0
4	37.62139334364276	39.0	37.0	39.0	34.0	40.0
5	38.42063800358571	40.0	38.0	41.0	34.0	41.0
6	38.420260025506586	40.0	38.0	41.0	34.0	41.0
7	38.45580659967593	40.0	38.0	41.0	34.0	41.0
8	38.353701998016156	40.0	38.0	41.0	34.0	41.0
9	38.24916980364855	40.0	38.0	41.0	34.0	41.0
10	38.240410076951036	40.0	38.0	41.0	34.0	41.0
11	38.185595400188525	40.0	37.0	41.0	34.0	41.0
12	38.333706587970006	40.0	38.0	41.0	34.0	41.0
13	38.49922925741324	40.0	38.0	41.0	34.0	41.0
14	38.52253313084141	40.0	38.0	41.0	34.0	41.0
15	38.496583718909996	40.0	38.0	41.0	34.0	41.0
16	38.530456346150295	40.0	38.0	41.0	34.0	41.0
17	38.54512740972577	40.0	38.0	41.0	34.0	41.0
18	38.557225172662356	40.0	38.0	41.0	34.0	41.0
19	38.53883409011096	40.0	38.0	41.0	34.0	41.0
20	38.52018809569284	40.0	38.0	41.0	34.0	41.0
21	38.44685711381237	40.0	38.0	41.0	34.0	41.0
22	38.379540665758945	40.0	38.0	41.0	34.0	41.0
23	38.33064040638035	40.0	38.0	41.0	34.0	41.0
24	38.2211136337032	40.0	37.0	41.0	33.0	41.0
25	38.12465690241817	40.0	37.0	41.0	33.0	41.0
26	38.079031840469845	40.0	37.0	41.0	33.0	41.0
27	38.06607386334416	40.0	37.0	41.0	33.0	41.0
28	38.03188957001019	40.0	37.0	41.0	33.0	41.0
29	37.98816931999867	40.0	37.0	41.0	33.0	41.0
30	37.98728116660651	40.0	37.0	41.0	33.0	41.0
31	37.87215675977287	40.0	36.0	41.0	33.0	41.0
32	37.7954300320143	40.0	36.0	41.0	33.0	41.0
33	37.769248120165955	40.0	36.0	41.0	33.0	41.0
34	37.70323440190211	39.0	36.0	41.0	33.0	41.0
35	37.64895170937688	39.0	36.0	41.0	33.0	41.0
36	37.645735073942106	39.0	36.0	41.0	33.0	41.0
37	37.561163702105226	39.0	35.0	41.0	33.0	41.0
38	37.47048200374617	39.0	35.0	41.0	32.0	41.0
39	37.3632880413737	39.0	35.0	41.0	32.0	41.0
40	37.299890461104475	39.0	35.0	41.0	32.0	41.0
41	37.256664038499075	39.0	35.0	41.0	32.0	41.0
42	37.126814571302845	39.0	35.0	40.0	32.0	41.0
43	37.056216117255154	39.0	35.0	40.0	32.0	41.0
44	36.9702906274188	38.0	35.0	40.0	31.0	41.0
45	36.850114652985	38.0	35.0	40.0	31.0	41.0
46	36.65831761912363	38.0	35.0	40.0	31.0	41.0
47	36.54844034169495	38.0	35.0	40.0	31.0	41.0
48	36.439360987338326	38.0	34.0	40.0	31.0	41.0
49	36.571789081296764	38.0	35.0	40.0	31.0	41.0
50	36.25350501139715	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	24.0
14	653.0
15	7780.0
16	6674.0
17	71.0
18	0.0
19	3.0
20	12.0
21	71.0
22	146.0
23	325.0
24	715.0
25	1467.0
26	3121.0
27	6561.0
28	13911.0
29	28317.0
30	55140.0
31	99675.0
32	165718.0
33	256954.0
34	365894.0
35	439740.0
36	600063.0
37	779295.0
38	978630.0
39	1374564.0
40	1306916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	56.16931384810642	12.503373154006814	31.327312997886775
2	25.037736197793127	27.975460689663674	30.35213263426385	16.634670478279354
3	26.232079156680694	20.493897517728314	25.99859220878437	27.275431116806626
4	21.64172483688721	23.204896772245874	29.519163827467022	25.634214563399894
5	19.774506965023935	26.75474859991005	29.135517617413488	24.33522681765253
6	20.422475987456178	31.215998915661906	25.771528115777738	22.58999698110418
7	26.56988743831287	29.382296948450815	22.77365674538386	21.274158867852456
8	24.95761223823401	27.822621387336653	24.637624683478016	22.58214169095132
9	21.884884573442342	28.357489634097504	27.899541620715784	21.85808417174437
10	24.733798078996493	25.774439193893205	24.91579437006734	24.57596835704296
11	23.74628028907468	27.677159896741443	25.308774513126036	23.267785301057845
12	23.842130231469216	25.487505467898046	27.395678666264146	23.274685634368588
13	21.484449606003288	25.611357209308057	27.435463400508898	25.468729784179757
14	20.125561422207983	26.467152565137297	27.465606151154265	25.941679861500454
15	18.958049670077813	29.571624843664324	27.966527222431015	23.50379826382685
16	22.892949337999273	34.69456783582135	22.340537609897048	20.071945216282323
17	23.62714172175638	31.94687975553105	22.120558680557696	22.305419842154876
18	22.112118094275804	27.51862165842118	22.36427290818244	28.00498733912058
19	22.25736394945506	28.756938839635023	23.871271817683336	25.114425393226586
20	24.877087812902392	25.484424961955753	25.079353833073544	24.559133392068315
21	23.32927219966607	28.1725976674409	24.406217077092744	24.091913055800283
22	23.101437980173863	29.05322190116505	24.52039602984394	23.324944088817148
23	24.930041710050457	26.811245078891755	25.254126337709703	23.004586873348078
24	24.05103474194602	28.14803063255109	24.332531374953025	23.468403250549873
25	23.062651362021843	29.29751903445715	24.29098332580624	23.34884627771477
26	23.92885295030434	28.58752602531195	24.793321184420776	22.69029983996294
27	22.961564368581673	28.219683428475385	24.913873554524486	23.90487864841846
28	22.875637696751337	27.301639633898176	25.040888311180236	24.781834358170247
29	24.062749568539164	27.75761030234807	26.00695100478378	22.172689124328993
30	23.59510651118177	27.19841645339184	25.638949647647134	23.56752738777925
31	21.61388696352273	29.086710339763304	25.4287988868702	23.87060380984376
32	22.791098980603174	27.75676646795832	26.084787844090574	23.367346707347934
33	23.089220426869865	27.52958425341452	26.090517246296763	23.290678073418853
34	21.845306222841682	28.504599491678285	26.514028039681893	23.136066245798148
35	22.091411919424523	28.000421204406933	25.486068565211422	24.422098310957125
36	23.205173663673666	28.462391208491905	26.757068489760872	21.57536663807356
37	21.92869847523712	28.73677184030049	25.122204093158324	24.212325591304065
38	22.99526932533612	29.082188530259657	24.769533176149054	23.153008968255172
39	22.72743834558064	29.491659793250115	24.51070227816687	23.270199583002373
40	20.61625101167311	30.34293969079924	25.925883573364267	23.114925724163378
41	22.537606154899095	28.990293549410556	24.856148665438276	23.61595163025207
42	22.110581330074115	29.64868128312347	25.45229206315754	22.788445323644872
43	21.895286738010007	27.707138835466882	26.05037054946276	24.34720387706035
44	22.280882614738136	28.64139978520922	25.910465731131133	23.167251868921518
45	23.33543609244221	27.881016294332056	26.291230531420197	22.49231708180553
46	22.323322231931815	30.63712665788294	25.075225297127073	21.96432581305817
47	24.22590790811787	28.730962185461994	24.087359720645342	22.955770185774792
48	22.348706452973506	29.50924571936289	24.683944912649935	23.45810291501367
49	21.96884363575408	29.213958581731802	24.337825035392015	24.479372747122106
50	23.47348093582225	31.347528903349126	24.306024238738456	20.87296592209017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40187.0
1	35339.0
2	28245.0
3	24787.0
4	23060.5
5	21537.0
6	19823.0
7	18425.0
8	17481.5
9	17134.0
10	16571.5
11	15792.5
12	15800.0
13	20283.0
14	25496.0
15	25731.0
16	24784.5
17	26953.0
18	29077.5
19	33836.0
20	38681.5
21	44616.5
22	51439.0
23	65390.5
24	78726.0
25	88768.0
26	101194.0
27	110176.5
28	115165.5
29	131253.0
30	153822.5
31	181021.0
32	209230.5
33	219104.0
34	226174.5
35	247388.5
36	270877.5
37	284114.5
38	298692.0
39	336550.0
40	370277.5
41	391149.0
42	422506.0
43	453134.0
44	474489.5
45	449359.5
46	426497.0
47	424170.0
48	409963.0
49	420862.0
50	464432.5
51	466287.5
52	420710.5
53	374360.5
54	345900.0
55	338852.0
56	336488.0
57	323182.0
58	295442.5
59	275227.5
60	267901.0
61	242731.0
62	210125.0
63	199143.0
64	190413.5
65	169247.5
66	143270.0
67	126480.5
68	114291.0
69	101933.5
70	88073.5
71	78070.0
72	69050.0
73	60125.0
74	52653.5
75	43395.0
76	33032.0
77	22738.0
78	16581.5
79	16807.5
80	16237.5
81	11474.5
82	8026.5
83	7164.0
84	5995.0
85	4572.0
86	3116.5
87	1956.5
88	999.5
89	434.0
90	263.5
91	224.0
92	192.5
93	167.0
94	112.0
95	38.5
96	18.0
97	18.0
98	40.0
99	61.5
100	61.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	40194.0
25	36857.0
26	37557.0
27	38612.0
28	40850.0
29	40013.0
30	43089.0
31	42402.0
32	37085.0
33	37281.0
34	39684.0
35	37551.0
36	36400.0
37	42795.0
38	41317.0
39	41647.0
40	43901.0
41	45316.0
42	45552.0
43	56496.0
44	60922.0
45	62574.0
46	71159.0
47	131633.0
48	425896.0
49	1005470.0
50	3910187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.955324607227977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.01902713337245	6.871068191877983
2	13.737353554431719	2.7351962775724963
3	5.486728958222219	1.6386650343293996
4	2.790438055093264	1.111188665392614
5	1.6215621321859153	0.8071588698349755
6	1.0684915955935839	0.6382308404537456
7	0.7444718026145688	0.518802091916933
8	0.5297657207124755	0.42191917723798167
9	0.39666219604025726	0.3554010828896995
>10	2.8136213685259	5.6719711730251925
>50	0.4525898306195813	3.190233949396877
>100	0.672978495118341	16.83678490627037
>500	0.5588449336064313	40.617262329193615
>1k	0.1045221197518263	14.677897978933169
>5k	0.0018581710178102453	1.312744521793391
>10k+	0.001083933093722643	2.5954749098816055
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	49414	0.7611006031630635	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	46112	0.7102414500557571	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	20929	0.322359544331561	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	17391	0.26786539421234545	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	12054	0.1856620931421777	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	11928	0.18372137439853123	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10539	0.16232726062928576	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	9683	0.14914269519625903	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	9597	0.14781807764107177	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	9282	0.1429662807819556	No Hit
ATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGG	7218	0.1111754594574613	No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA	7008	0.10794092821805053	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	6851	0.10552273105334821	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	6749	0.10395167302277726	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	6641	0.10228819981393743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.049241887487600965	0.0	0.0	0.0	0.0
2	0.1076636826832439	0.0	0.0	0.0	0.0
3	0.1410717696274436	0.0	0.0	0.0	0.0
4	0.18241215937305544	0.0	0.0	0.0	0.0
5	0.22784962202192088	0.0	0.0	0.0	0.0
6	0.30806599675930774	0.0	0.0	0.0	0.0
7	0.3778548588820228	0.0	0.0	0.0	0.0
8	0.4634775215481391	0.0	0.0	0.0	0.0
9	0.539843263857656	0.0	0.0	0.0	0.0
10	0.6273450351485728	0.0	0.0	0.0	0.0
11	0.7174036263715953	0.0	0.0	0.0	0.0
12	0.7983285174757102	0.0	0.0	0.0	0.0
13	0.8857224710586467	0.0	0.0	0.0	0.0
14	0.9773367177825286	0.0	0.0	0.0	0.0
15	1.0736025284792774	0.0	0.0	0.0	0.0
16	1.1730566628263026	0.0	0.0	0.0	0.0
17	1.2816290947625237	0.0	0.0	0.0	0.0
18	1.3686687901620962	0.0	0.0	0.0	0.0
19	1.4700944483121907	0.0	0.0	0.0	0.0
20	1.5604918951888658	0.0	0.0	0.0	0.0
21	1.6451904060722933	0.0	0.0	0.0	0.0
22	1.7291649980592814	0.0	0.0	0.0	0.0
23	1.7983377589935372	0.0	0.0	0.0	0.0
24	1.8692818108446132	0.0	0.0	0.0	0.0
25	1.9412116245972237	0.0	0.0	0.0	0.0
26	2.0214896094534565	0.0	0.0	0.0	0.0
27	2.0894763755999284	0.0	0.0	0.0	0.0
28	2.155907486245541	0.0	0.0	0.0	0.0
29	2.23895792644984	0.0	0.0	0.0	0.0
30	2.30557386745199	0.0	0.0	0.0	0.0
31	2.377780926739408	0.0	0.0	0.0	0.0
32	2.4432262754834855	0.0	0.0	0.0	0.0
33	2.5113824694567835	0.0	0.0	0.0	0.0
34	2.5863003739734216	0.0	0.0	0.0	0.0
35	2.664006136367837	0.0	0.0	0.0	0.0
36	2.7398173876077405	0.0	0.0	0.0	0.0
37	2.807557713278829	0.0	0.0	0.0	0.0
38	2.8765918514456814	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAT	120	0.0	62.998493	44
ATATCGA	125	0.0	57.72953	44
TAAGCCA	6310	0.0	56.036983	44
TATCGTA	80	0.0	55.839573	44
TGTACGG	95	0.0	47.0228	44
TAACGCT	125	0.0	45.544495	38
TCGATTG	60	0.0	44.959003	31
TATCGCG	95	0.0	44.432304	29
TCCCGTT	210	0.0	44.39233	36
CTCGTAC	200	0.0	44.28135	36
CGCGTTA	55	2.7284841E-11	44.0806	40
CGTAAGT	80	0.0	44.046364	37
GATGCGC	70	0.0	43.626873	37
TCTAGCG	25	4.880679E-5	43.308598	25
TCATGCG	160	0.0	43.306038	30
TAACGTC	680	0.0	43.208714	35
GACGCAT	60	0.0	43.036926	24
CATACGC	345	0.0	42.99057	42
CGCGTCA	50	6.4574124E-10	42.697964	38
GCGCGTC	50	7.0940587E-10	42.28451	37
>>END_MODULE