##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840890.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5857675
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.94511696193456	39.0	37.0	39.0	32.0	39.0
2	37.42567674034493	39.0	37.0	39.0	35.0	39.0
3	37.44520650257995	39.0	37.0	39.0	34.0	39.0
4	37.61238443580431	39.0	37.0	39.0	34.0	40.0
5	38.32908688174062	40.0	38.0	41.0	34.0	41.0
6	38.3038729530061	40.0	38.0	41.0	34.0	41.0
7	38.28656403777949	40.0	38.0	41.0	34.0	41.0
8	38.19543743891561	40.0	38.0	41.0	33.0	41.0
9	38.05274328125067	39.0	37.0	40.0	33.0	41.0
10	38.01202524892555	39.0	37.0	40.0	33.0	41.0
11	37.879382519515	39.0	37.0	40.0	33.0	41.0
12	38.06204099749474	39.0	37.0	41.0	33.0	41.0
13	38.25930970905692	40.0	38.0	41.0	34.0	41.0
14	38.24528469059823	40.0	37.0	41.0	34.0	41.0
15	38.27118455701281	40.0	38.0	41.0	34.0	41.0
16	38.28169111464873	40.0	38.0	41.0	34.0	41.0
17	38.267183481500766	40.0	37.0	41.0	34.0	41.0
18	38.275923638645025	40.0	37.0	41.0	34.0	41.0
19	38.25058815997815	40.0	37.0	41.0	33.0	41.0
20	38.18313921479085	40.0	37.0	41.0	33.0	41.0
21	38.13331944841597	40.0	37.0	41.0	33.0	41.0
22	37.97243377278528	40.0	37.0	41.0	33.0	41.0
23	37.91113624432902	40.0	37.0	41.0	33.0	41.0
24	37.72561536787207	39.0	36.0	41.0	32.0	41.0
25	37.63992154898584	39.0	36.0	41.0	32.0	41.0
26	37.5585974743946	39.0	36.0	41.0	32.0	41.0
27	37.49082360996465	39.0	35.0	41.0	32.0	41.0
28	37.43511704754433	39.0	35.0	41.0	32.0	41.0
29	37.38530834812546	39.0	35.0	41.0	32.0	41.0
30	37.34480832894052	39.0	35.0	41.0	32.0	41.0
31	37.17381672313541	39.0	35.0	41.0	31.0	41.0
32	37.08465008359478	39.0	35.0	41.0	31.0	41.0
33	37.05027922975449	39.0	35.0	40.0	31.0	41.0
34	36.991440270585905	38.0	35.0	40.0	31.0	41.0
35	36.933413430520254	38.0	35.0	40.0	31.0	41.0
36	36.8645957375593	38.0	35.0	40.0	31.0	41.0
37	36.78203058412546	38.0	35.0	40.0	31.0	41.0
38	36.71389423323511	38.0	35.0	40.0	31.0	41.0
39	36.568040947826276	38.0	35.0	40.0	31.0	41.0
40	36.539632965458566	38.0	34.0	40.0	31.0	41.0
41	36.42076908319334	38.0	34.0	40.0	31.0	41.0
42	36.26826719392743	37.0	34.0	40.0	30.0	41.0
43	36.195923374047396	37.0	34.0	40.0	30.0	41.0
44	36.109350809627365	37.0	34.0	40.0	30.0	41.0
45	35.9853357844714	37.0	34.0	40.0	30.0	41.0
46	35.849137344372494	37.0	34.0	40.0	30.0	41.0
47	35.7383600396254	36.0	34.0	40.0	30.0	41.0
48	35.60267753846853	36.0	34.0	40.0	29.0	41.0
49	35.77520074734701	36.0	34.0	40.0	30.0	41.0
50	35.84109642264564	37.0	34.0	39.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	102.0
15	1100.0
16	743.0
17	8.0
18	1.0
19	0.0
20	4.0
21	31.0
22	68.0
23	161.0
24	430.0
25	1233.0
26	2997.0
27	7241.0
28	16688.0
29	36006.0
30	69952.0
31	123779.0
32	199497.0
33	299210.0
34	415061.0
35	510559.0
36	648838.0
37	770936.0
38	857505.0
39	1064513.0
40	831008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.104734386936805	14.9276803509925	32.967585262070706
2	26.00545438249818	22.968293734288775	31.824930539847294	19.201321343365755
3	25.392275945661037	16.95706914432774	29.461552578454764	28.189102331556466
4	21.80308740242502	18.974268801188185	33.086813454143496	26.135830342243295
5	19.07898611650527	25.30809578885821	30.050950249032255	25.561967845604272
6	22.142658990128336	26.698220710435454	27.940966339033828	23.218153960402375
7	27.717652481573317	26.625188321304954	23.591988288868876	22.06517090825285
8	26.969625320626356	23.092609269035925	24.42192508119689	25.515840329140826
9	25.5435817111738	21.989355845109195	27.383304809502064	25.083757634214944
10	27.52547725846859	20.94267776891002	26.38485405899098	25.146990913630408
11	27.002385758854835	22.34878172653826	27.087504854741855	23.56132765986505
12	26.726423026200667	21.86756691007951	26.369165240475102	25.03684482324472
13	24.69394426969745	21.8805584126808	27.794321125702602	25.631176191919145
14	21.434237986914603	24.699851050118006	27.479520458202273	26.386390504765117
15	22.23201184770408	24.948311403415175	27.518443751146997	25.30123299773374
16	24.301587233842778	29.002104077129577	22.828289381025748	23.868019308001895
17	23.989279022820487	28.645699872389642	23.149235831622615	24.215785273167256
18	23.672344402856083	23.544819403602965	24.44556927449884	28.337266919042108
19	24.766174292701457	23.670944530039648	24.510356071308156	27.052525105950743
20	25.447109988177903	22.016841152846478	25.869444788247897	26.666604070727722
21	25.29756259949553	23.56238610028723	24.88927774244901	26.250773557768227
22	25.41452026614655	24.135497445658903	26.09644611556633	24.353536172628218
23	26.547341735415504	24.85066173865911	25.604305462491517	22.99769106343387
24	26.168112775119823	23.65964311778991	25.698455445206502	24.47378866188377
25	24.890090266859094	26.69857719326781	24.87552915277896	23.535803387094138
26	25.541028852905335	25.276983240262247	26.46629049494799	22.71569741188443
27	25.73428167832515	24.75535017677872	25.896469443709602	23.61389870118653
28	24.886568449781738	25.122453913696862	25.670421520592484	24.320556115928913
29	26.209968203217983	25.369699551767887	24.855606863357696	23.564725381656434
30	26.102722764523783	24.811479231794316	25.00717317741778	24.07862482626412
31	23.585425424383217	26.942513950888824	26.04230383799472	23.429756786733243
32	25.577483433548448	24.56788053525501	27.037387707749566	22.817248323446975
33	25.352112156992963	24.39112029664119	26.260458792904153	23.996308753461694
34	24.923677419186927	26.14941193223752	26.514135525702798	22.412775122872755
35	23.712193277537878	24.446684231532135	25.877161723797492	25.963960767132495
36	24.511627678034916	25.614400835419293	27.424520146955643	22.449451339590144
37	23.620981581492057	26.01768085033195	27.200512920474395	23.160824647701602
38	23.140447104855493	25.52322852758666	27.300919407516073	24.035404960041774
39	24.36820273556326	25.652307761763765	26.757305940338195	23.222183562334784
40	24.80016600400522	25.341406593397963	26.311654458815358	23.546772943781455
41	24.42665396131792	26.3100805367957	26.788493203246205	22.474772298640175
42	24.140411195352314	27.13218908942453	26.376659905801503	22.35073980942165
43	24.61288865905797	25.649697213947498	26.833913170849343	22.903500956145184
44	24.514546630757746	27.090886488599626	25.66781274254267	22.72675413809996
45	23.978050633639324	26.646507593680628	26.441841564000956	22.93360020867909
46	23.781965150679785	26.20550432780096	26.069713320542064	23.942817200977192
47	24.323236346429457	26.332314613397063	25.754614452376334	23.58983458779715
48	23.156226343265924	26.374720921480815	25.645624197253614	24.823428537999646
49	22.649513354883865	27.584293928613345	24.244491950861743	25.521700765641043
50	22.707458208998002	29.76210418371799	26.008891634793297	21.521545972490706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9655.0
1	7735.0
2	7069.5
3	8194.0
4	7987.0
5	7141.0
6	5711.0
7	5638.5
8	6822.0
9	8376.5
10	9567.0
11	11246.5
12	12955.5
13	14020.0
14	16018.0
15	18857.0
16	21565.0
17	26517.5
18	30192.0
19	30929.0
20	31401.0
21	40823.0
22	50782.0
23	57034.0
24	62892.0
25	76636.5
26	93652.5
27	103268.5
28	109660.5
29	129889.0
30	156124.5
31	170838.0
32	181511.5
33	188321.0
34	198592.5
35	211628.5
36	218495.0
37	217227.0
38	226899.5
39	264793.5
40	296308.0
41	298472.5
42	298429.0
43	295798.0
44	290875.0
45	293480.5
46	303465.5
47	302607.0
48	301876.0
49	314600.0
50	339657.5
51	354959.0
52	365962.0
53	388295.5
54	379254.0
55	346123.5
56	337308.5
57	331233.5
58	320620.5
59	314629.0
60	292354.5
61	268580.0
62	255379.0
63	243073.0
64	236328.5
65	224433.0
66	203305.5
67	194702.0
68	193947.0
69	185340.5
70	161173.5
71	136564.0
72	118349.0
73	106634.0
74	99130.5
75	85861.0
76	71010.5
77	55950.0
78	42306.0
79	35005.0
80	27126.5
81	18714.5
82	12578.0
83	9897.5
84	8140.0
85	6205.5
86	4459.0
87	3103.5
88	2193.0
89	1713.0
90	1180.5
91	609.0
92	401.0
93	277.0
94	267.5
95	222.5
96	171.5
97	169.5
98	225.0
99	281.0
100	281.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	47680.0
25	47733.0
26	42965.0
27	44509.0
28	46231.0
29	41772.0
30	54521.0
31	57042.0
32	48329.0
33	47751.0
34	47286.0
35	49718.0
36	49358.0
37	46614.0
38	47334.0
39	44979.0
40	48209.0
41	48749.0
42	54131.0
43	51872.0
44	56675.0
45	65860.0
46	68341.0
47	118868.0
48	372126.0
49	1068992.0
50	3140030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.639130503893146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.66184208009835	6.618404564477699
2	13.17399259323325	2.5397166772699395
3	5.434204415237213	1.5714301662991148
4	2.7436595934578993	1.057859715183964
5	1.6728072933739884	0.8062203904348069
6	1.0973252110680227	0.6346356548818057
7	0.8202515989744615	0.5534558545979301
8	0.5947667893625089	0.4586427761637399
9	0.4843919290369073	0.4202205317117367
>10	3.347361983528397	6.5688441075595865
>50	0.5145091998785704	3.521341738417437
>100	0.7460611015774562	17.796748002504916
>500	0.6020945005363082	42.32052773352386
>1k	0.10478146343255539	13.313161999301728
>5k	0.0012410664027544633	0.775736377009335
>10k+	7.091808015739791E-4	1.043053710662402
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	23662	0.4039486656395242	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	13157	0.22461130055866874	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12537	0.21402689633685718	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG	11678	0.1993623750037344	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	8313	0.1419163746708378	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	7252	0.12380338615577	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	7112	0.12141335939600609	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	6045	0.10319794116266266	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG	5980	0.10208828588134371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03528703794594271	0.0	0.0	0.0	0.0
2	0.06630617096373562	0.0	0.0	0.0	0.0
3	0.09092344658930378	0.0	0.0	0.0	0.0
4	0.11985984200215956	0.0	0.0	0.0	0.0
5	0.15362750579368095	0.0	0.0	0.0	0.0
6	0.20634466746618751	0.0	0.0	0.0	0.0
7	0.25755952660398534	0.0	0.0	0.0	0.0
8	0.31707119292210645	0.0	0.0	0.0	0.0
9	0.3702834315662784	0.0	0.0	0.0	0.0
10	0.4286171561242302	0.0	0.0	0.0	0.0
11	0.4852095754714968	0.0	0.0	0.0	0.0
12	0.5439188756631257	0.0	0.0	0.0	0.0
13	0.6034134703615343	0.0	0.0	0.0	0.0
14	0.6664248187207382	0.0	0.0	0.0	0.0
15	0.7344552232754463	0.0	0.0	0.0	0.0
16	0.7969714946629849	0.0	0.0	0.0	0.0
17	0.8679382178082601	0.0	0.0	0.0	0.0
18	0.9282181070134482	0.0	0.0	0.0	0.0
19	0.9998506233275147	0.0	0.0	0.0	0.0
20	1.0655422159815968	0.0	0.0	0.0	0.0
21	1.1315410977905056	0.0	0.0	0.0	0.0
22	1.1897723926301818	0.0	0.0	0.0	0.0
23	1.245152726977854	0.0	0.0	0.0	0.0
24	1.3003623451283999	0.0	0.0	0.0	0.0
25	1.3545135228567649	0.0	0.0	0.0	0.0
26	1.4133935392455197	0.0	0.0	0.0	0.0
27	1.4634133850034357	0.0	0.0	0.0	0.0
28	1.5157378994225525	0.0	0.0	0.0	0.0
29	1.5778785951764138	0.0	0.0	0.0	0.0
30	1.6313639797359873	0.0	0.0	0.0	0.0
31	1.687529608590439	0.0	0.0	0.0	0.0
32	1.7425002240650087	0.0	0.0	0.0	0.0
33	1.7975903408775666	0.0	0.0	0.0	0.0
34	1.854746123675349	0.0	0.0	0.0	0.0
35	1.9120555510505448	0.0	0.0	0.0	0.0
36	1.9717550051855044	0.0	0.0	0.0	0.0
37	2.0281937799553575	0.0	0.0	0.0	0.0
38	2.0858104964853803	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATACG	60	0.0	69.79501	44
TATACCG	55	0.0	69.2182	44
TTACCCG	95	0.0	64.117905	44
ACTAGCC	115	0.0	62.89827	44
CGTTAGC	110	0.0	56.806293	43
AACCGAC	135	0.0	56.40001	44
ACATACG	70	3.6379788E-12	54.385727	44
CGAACCG	95	0.0	53.816486	43
ACGGATA	25	1.8969451E-5	50.819714	41
TAAGCCA	3735	0.0	50.55616	44
CGCACTA	40	2.6338967E-9	50.111378	40
ATATCGT	180	0.0	48.84711	38
TTACGTT	100	0.0	48.84711	38
CCCGTTA	55	0.0	47.791477	36
ACGATAC	45	2.3283064E-10	47.35257	35
CGGTAAC	140	0.0	47.35257	35
AACGATA	45	2.564775E-10	46.905514	34
TAAGGCG	90	0.0	46.905514	34
CGTTATA	75	0.0	46.90551	34
CGTCGTT	140	0.0	45.230316	34
>>END_MODULE