FastQCFastQC Report
Tue 24 May 2016
ERR840887.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840887.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4956818
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA838841.692295339469797No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG330830.667424141858749No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC293110.5913269359496354No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT279760.5643943352368395No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT226130.4561999250325511No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC189240.38177718044116205No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA176840.3567611318390145No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA156260.3152425608525469No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC151580.30580101992851055No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT141250.2849610375043022No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA111020.22397433192019559No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG106490.21483540448731425No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG88340.17821917205755788No Hit
CCGATCGCACGCCCCCCGTGGCGGCG79880.16115177115641527No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC77050.1554424632899574No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG76320.15396974429966967No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC69310.1398276071463588No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG68740.13867767587996976No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC66850.13486474589141664No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG63920.12895369569752207No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA63570.12824759755149373No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG62340.12576616692402265No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA61270.12360752402045022No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC58370.11775699652478668No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT54530.11001009115121838No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG54330.10960660649634503No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT54040.10902155374677867No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG52850.10662082005028226No Hit
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA51940.10478496487060851No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA50850.10258597350154877No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT49830.1005282017616947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAC600.099.7713244
TAAGCCA105500.095.1201944
TCGTTTA750.079.81705544
CTATCAT40950.078.8070544
ATCCATA2400.065.7583744
GATATGT356.3100742E-965.46850643
CCTATAG750.065.46850643
CGGATTA2000.065.3048644
TTAATAC1600.064.62459644
ACGCGTT1100.062.4926643
GCCTATG1800.060.4674744
TTAAGCC100300.059.75714543
TACGGGA553.203004E-759.36805744
TATAAGC1550.056.17622844
TCCAATG1400.054.4207244
ACTTCTA34800.053.63881344
GAGTAAT1850.052.94988644
GACGCAC501.7807906E-952.37480543
CATAACA1100.051.81667742
ATCGTTG550.051.7124841
Produced by FastQC (version 0.11.5)