##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840887.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4956818
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.52182408149744	38.0	35.0	39.0	30.0	39.0
2	36.29486073525395	38.0	35.0	39.0	32.0	39.0
3	36.46047847631283	38.0	35.0	39.0	33.0	39.0
4	36.85596949494615	38.0	35.0	39.0	33.0	40.0
5	37.42249705355331	39.0	35.0	40.0	33.0	41.0
6	37.42500087757912	39.0	35.0	40.0	33.0	41.0
7	37.311267430032736	39.0	35.0	40.0	33.0	41.0
8	37.19073990612526	38.0	35.0	40.0	33.0	41.0
9	37.06532255168538	38.0	35.0	40.0	32.0	41.0
10	37.00833276509245	38.0	35.0	40.0	32.0	41.0
11	36.918638328056424	38.0	35.0	40.0	32.0	41.0
12	37.07347798527201	38.0	35.0	40.0	33.0	41.0
13	37.150459226059944	38.0	35.0	40.0	33.0	41.0
14	37.153055448071726	38.0	35.0	40.0	33.0	41.0
15	37.123607927505105	38.0	35.0	40.0	33.0	41.0
16	37.07530133242737	38.0	35.0	40.0	32.0	41.0
17	37.053211556284694	38.0	35.0	40.0	32.0	41.0
18	36.997843374519704	38.0	35.0	40.0	32.0	41.0
19	36.852996014782065	38.0	35.0	40.0	32.0	41.0
20	36.770965970507696	38.0	35.0	40.0	32.0	41.0
21	36.61721854625286	38.0	35.0	40.0	32.0	41.0
22	36.45450347380114	37.0	35.0	40.0	31.0	41.0
23	36.293796140992065	37.0	35.0	40.0	31.0	41.0
24	36.08212869627249	37.0	34.0	40.0	31.0	41.0
25	35.90144239878252	36.0	34.0	40.0	31.0	41.0
26	35.76413246347255	36.0	34.0	40.0	30.0	41.0
27	35.640356188987234	36.0	34.0	40.0	30.0	41.0
28	35.62582350909705	35.0	34.0	39.0	30.0	41.0
29	35.50868274082984	35.0	34.0	39.0	30.0	41.0
30	35.415156960829776	35.0	34.0	39.0	30.0	41.0
31	35.28037477234349	35.0	34.0	39.0	30.0	41.0
32	35.205699307646455	35.0	34.0	39.0	30.0	41.0
33	35.15762884462816	35.0	34.0	39.0	30.0	41.0
34	35.09432984395969	35.0	34.0	39.0	30.0	41.0
35	35.021269789277326	35.0	34.0	38.0	30.0	41.0
36	34.966516612140644	35.0	34.0	38.0	30.0	41.0
37	34.9100936084882	35.0	33.0	38.0	30.0	41.0
38	34.8012011347138	35.0	33.0	38.0	30.0	40.0
39	34.711294500213	35.0	33.0	37.0	30.0	40.0
40	34.58499871340986	35.0	33.0	37.0	29.0	40.0
41	34.516454273463346	35.0	33.0	37.0	29.0	40.0
42	34.455100048981016	35.0	33.0	37.0	29.0	40.0
43	34.36048793790255	35.0	33.0	36.0	29.0	40.0
44	34.24794574761537	35.0	33.0	36.0	29.0	40.0
45	34.046651815983545	35.0	33.0	36.0	29.0	39.0
46	33.93135153842485	35.0	33.0	36.0	29.0	39.0
47	33.90088731816063	35.0	33.0	36.0	29.0	39.0
48	33.826794579752665	35.0	33.0	36.0	29.0	39.0
49	33.952081581195245	35.0	33.0	36.0	29.0	39.0
50	34.25263819226335	35.0	33.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	12.0
14	129.0
15	654.0
16	314.0
17	4.0
18	1.0
19	2.0
20	26.0
21	134.0
22	430.0
23	1130.0
24	2620.0
25	5975.0
26	12438.0
27	25097.0
28	47756.0
29	85127.0
30	141818.0
31	222138.0
32	326201.0
33	473384.0
34	656742.0
35	485552.0
36	585463.0
37	622145.0
38	539235.0
39	459106.0
40	263185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.2023858854612	15.929130341279427	38.86848377325938
2	26.542310006137004	21.218451030479635	29.840756711261136	22.39848225212223
3	23.376569404000712	17.0996796735325	28.204243125327576	31.319507797139213
4	19.182971817807314	18.92667836503176	33.221231846721025	28.669117970439906
5	20.25499019734031	20.666867333035025	33.72294484082329	25.355197628801378
6	23.888490559871272	24.42102978160586	27.086852896354074	24.603626762168794
7	29.16134907515265	21.157444150662783	22.322304349282142	27.358902424902425
8	25.41816140919437	20.47527264466841	26.076527320551207	28.030038625586013
9	23.499470829875133	23.275678066049633	27.629983590279082	25.59486751379615
10	28.419865324891898	18.649565104064745	23.66122782801386	29.269341743029496
11	26.92192854367459	22.10341392401335	25.3737780971583	25.60087943515376
12	26.328140351330227	20.035575242020183	24.858891329074417	28.77739307757517
13	24.124831696463335	19.77310847402507	26.617600242736366	29.484459586775223
14	22.291740386675485	22.508310775178753	23.637785369565716	31.562163468580046
15	22.96366338243607	23.769059102028763	26.685486535918805	26.58179097961636
16	26.252426455843242	25.78773317882561	21.20253356084488	26.757306804486262
17	26.113970696523452	25.27978634680555	20.73995857826533	27.866284378405666
18	25.2025190353973	22.77509482898101	20.286845310842562	31.735540824779125
19	24.186685894055422	23.279248905245257	24.38267856516015	28.151386635539172
20	30.302484376065454	19.71315065431089	23.38336812043533	26.60099684918833
21	28.292061560460763	22.77317827687036	22.40568445321172	26.529075709457157
22	26.81084921818796	23.627758775892115	22.260046667035184	27.30134533888474
23	29.9604907825948	22.88316819378884	21.961790810152802	25.194550213463557
24	28.601453593817645	24.36696283785283	22.07702602758463	24.95455754074489
25	28.360310400958337	23.495820506917607	24.2150029846246	23.928866107499456
26	28.581589316708815	22.70754239667115	24.803239413522135	23.9076288730979
27	26.692528161975996	24.824106447875703	22.216255640756494	26.26710974939181
28	26.772519523186162	21.793249967027133	25.414708476888485	26.01952203289822
29	31.92029220208753	23.48669116361344	21.85160676007345	22.741409874225578
30	28.178673156930916	25.873974054613097	21.12066036586799	24.826692422588
31	26.166790235946415	22.748935227308802	25.757942574731697	25.326331962013082
32	24.329884302067892	22.592908780073493	27.01733394759423	26.059872970264387
33	28.04476331698314	20.891216837811054	26.280561222137894	24.78345862306791
34	25.961256299288305	23.747021784948195	25.27157377428333	25.02014814148017
35	24.88560396158993	22.406753168219094	24.640704202665237	28.066938667525744
36	25.266459290493472	22.29016572365511	28.901315397703588	23.54205958814783
37	23.929675986847386	24.554305903311132	23.941945216399397	27.574072893442086
38	25.48478815132389	24.156184567697807	24.921738884744656	25.437288396233647
39	25.04985969143807	25.715793445969865	23.234218735460026	26.000128127132044
40	23.327072479328905	26.58593795965471	23.148414847699154	26.938574713317237
41	26.01233780920652	22.92432684566179	23.933410509325725	27.12992483580597
42	24.92675177148822	24.298664959452456	24.589340388145	26.185242880914327
43	25.60608600417456	21.255833365506746	26.700136789947038	26.437943840371652
44	25.716809928538392	23.525129474569688	25.074918629580495	25.683141967311425
45	26.055429126171738	23.82379105504938	26.76206062377381	23.358719195005076
46	25.27985909684567	26.49613324794676	23.61895245360165	24.60505520160592
47	25.60077885747288	23.184687670404326	23.236408674462698	27.978124797660097
48	24.592754567335547	22.164038230735432	23.98252858888624	29.260678613042785
49	23.668355851222287	25.000403231779273	23.047181409862887	28.284059507135556
50	24.162217369828255	30.57123324695146	22.684204726680047	22.582344656540236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16541.0
1	11865.5
2	6049.0
3	4103.0
4	3230.5
5	3510.5
6	3687.5
7	3653.0
8	3703.5
9	3443.5
10	3120.0
11	3108.0
12	3410.0
13	4910.5
14	7053.0
15	7055.5
16	5820.0
17	7655.5
18	10286.0
19	12751.5
20	15350.0
21	18028.0
22	20233.0
23	24920.5
24	29098.0
25	30926.5
26	35035.0
27	37304.5
28	38941.5
29	43341.5
30	46555.5
31	58827.0
32	80023.0
33	91392.0
34	96652.5
35	115012.0
36	127193.0
37	120483.5
38	119842.5
39	145099.0
40	174214.0
41	192387.0
42	216356.5
43	260270.5
44	288335.0
45	271052.0
46	280694.5
47	287875.5
48	280144.5
49	320895.0
50	375347.5
51	379593.0
52	347561.0
53	328811.5
54	339855.5
55	344784.0
56	332676.0
57	342461.0
58	347956.5
59	323776.5
60	304258.0
61	300497.5
62	294875.5
63	283538.5
64	264493.5
65	240978.0
66	211188.0
67	189765.0
68	178553.0
69	165982.5
70	146650.0
71	133195.5
72	130753.0
73	116024.0
74	96185.5
75	86164.5
76	77846.5
77	61217.5
78	46749.5
79	43696.0
80	42015.5
81	34619.0
82	27074.5
83	25929.5
84	24423.5
85	19256.5
86	15239.5
87	8958.5
88	3273.0
89	1945.5
90	1140.5
91	551.0
92	384.5
93	362.5
94	189.5
95	38.5
96	58.0
97	58.0
98	30.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	48328.0
25	42424.0
26	52217.0
27	44776.0
28	45219.0
29	44024.0
30	49499.0
31	50301.0
32	46139.0
33	45884.0
34	42923.0
35	45350.0
36	39232.0
37	42585.0
38	40915.0
39	44885.0
40	45150.0
41	44546.0
42	50015.0
43	47017.0
44	55898.0
45	68500.0
46	75358.0
47	186169.0
48	621509.0
49	1213884.0
50	1824071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.303094019607371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.49183328580418	5.520879433080911
2	13.253714834391046	2.200936807580276
3	5.622827350727576	1.4006059244733269
4	3.00133583959889	0.9968149464242725
5	1.8240959420076597	0.7572820053636936
6	1.253258251312169	0.6243552654896198
7	0.8997934220521802	0.5229748567066456
8	0.6913633132842473	0.4592363673525097
9	0.5572230029848239	0.4164007485303856
>10	4.122611432020297	6.957385778271051
>50	0.6395962710814678	3.7498810530771762
>100	0.9551066416790898	19.578218275532198
>500	0.568700532285836	33.43461513179667
>1k	0.11126749544722878	15.015615622690062
>5k	0.004363431194008971	2.323750626514973
>10k+	0.0029089541293393145	6.041047157116253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	83884	1.692295339469797	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	33083	0.667424141858749	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	29311	0.5913269359496354	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	27976	0.5643943352368395	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	22613	0.4561999250325511	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	18924	0.38177718044116205	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	17684	0.3567611318390145	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	15626	0.3152425608525469	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	15158	0.30580101992851055	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	14125	0.2849610375043022	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	11102	0.22397433192019559	No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG	10649	0.21483540448731425	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	8834	0.17821917205755788	No Hit
CCGATCGCACGCCCCCCGTGGCGGCG	7988	0.16115177115641527	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	7705	0.1554424632899574	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	7632	0.15396974429966967	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	6931	0.1398276071463588	No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG	6874	0.13867767587996976	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	6685	0.13486474589141664	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	6392	0.12895369569752207	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	6357	0.12824759755149373	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	6234	0.12576616692402265	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	6127	0.12360752402045022	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	5837	0.11775699652478668	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT	5453	0.11001009115121838	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG	5433	0.10960660649634503	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	5404	0.10902155374677867	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	5285	0.10662082005028226	No Hit
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA	5194	0.10478496487060851	No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA	5085	0.10258597350154877	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	4983	0.1005282017616947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.016159560427677595	0.0	0.0	0.0	0.0
2	0.03167354540755783	0.0	0.0	0.0	0.0
3	0.04652178070689705	0.0	0.0	0.0	0.0
4	0.06300412885847331	0.0	0.0	0.0	0.0
5	0.08446951249773545	0.0	0.0	0.0	0.0
6	0.11789821615399233	0.0	0.0	0.0	0.0
7	0.14951123886331916	0.0	0.0	0.0	0.0
8	0.18782210684354358	0.0	0.0	0.0	0.0
9	0.22342962763611657	0.0	0.0	0.0	0.0
10	0.2600862085313602	0.0	0.0	0.0	0.0
11	0.3009592040700304	0.0	0.0	0.0	0.0
12	0.336344808302423	0.0	0.0	0.0	0.0
13	0.37677397072073254	0.0	0.0	0.0	0.0
14	0.41774783742312105	0.0	0.0	0.0	0.0
15	0.4657423371203058	0.0	0.0	0.0	0.0
16	0.5098835583634501	0.0	0.0	0.0	0.0
17	0.5644750321678141	0.0	0.0	0.0	0.0
18	0.6084750337817527	0.0	0.0	0.0	0.0
19	0.6608876904498007	0.0	0.0	0.0	0.0
20	0.7097698563877068	0.0	0.0	0.0	0.0
21	0.7582283634379959	0.0	0.0	0.0	0.0
22	0.805113280334279	0.0	0.0	0.0	0.0
23	0.844049549529557	0.0	0.0	0.0	0.0
24	0.887787286117828	0.0	0.0	0.0	0.0
25	0.9338854079371081	0.0	0.0	0.0	0.0
26	0.9772398341032493	0.0	0.0	0.0	0.0
27	1.0221073277251655	0.0	0.0	0.0	0.0
28	1.0684071918718823	0.0	0.0	0.0	0.0
29	1.1206786289107247	0.0	0.0	0.0	0.0
30	1.166030304118489	0.0	0.0	0.0	0.0
31	1.2103934419218136	0.0	0.0	0.0	0.0
32	1.2567538287667612	0.0	0.0	0.0	0.0
33	1.303477351801095	0.0	0.0	0.0	0.0
34	1.351633245360229	0.0	0.0	0.0	0.0
35	1.4003136689706985	0.0	0.0	0.0	0.0
36	1.4539569538361101	0.0	0.0	0.0	0.0
37	1.5002164695173394	0.0	0.0	0.0	0.0
38	1.5418762601330127	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATAC	60	0.0	99.77132	44
TAAGCCA	10550	0.0	95.12019	44
TCGTTTA	75	0.0	79.817055	44
CTATCAT	4095	0.0	78.80705	44
ATCCATA	240	0.0	65.75837	44
GATATGT	35	6.3100742E-9	65.468506	43
CCTATAG	75	0.0	65.468506	43
CGGATTA	200	0.0	65.30486	44
TTAATAC	160	0.0	64.624596	44
ACGCGTT	110	0.0	62.49266	43
GCCTATG	180	0.0	60.46747	44
TTAAGCC	10030	0.0	59.757145	43
TACGGGA	55	3.203004E-7	59.368057	44
TATAAGC	155	0.0	56.176228	44
TCCAATG	140	0.0	54.42072	44
ACTTCTA	3480	0.0	53.638813	44
GAGTAAT	185	0.0	52.949886	44
GACGCAC	50	1.7807906E-9	52.374805	43
CATAACA	110	0.0	51.816677	42
ATCGTTG	55	0.0	51.71248	41
>>END_MODULE