##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840886.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9038221
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.02429460399342	39.0	37.0	39.0	33.0	39.0
2	37.460641092976154	39.0	37.0	39.0	35.0	39.0
3	37.46789229871675	39.0	37.0	39.0	34.0	39.0
4	37.70985606570142	39.0	37.0	39.0	34.0	40.0
5	38.61020514988514	40.0	38.0	41.0	34.0	41.0
6	38.548887220173086	40.0	38.0	41.0	34.0	41.0
7	38.60280966796453	40.0	38.0	41.0	35.0	41.0
8	38.53898991848064	40.0	38.0	41.0	34.0	41.0
9	38.479081447554776	40.0	38.0	41.0	34.0	41.0
10	38.455131380390014	40.0	38.0	41.0	34.0	41.0
11	38.3769902284974	40.0	38.0	41.0	34.0	41.0
12	38.516611178239614	40.0	38.0	41.0	34.0	41.0
13	38.62572645656706	40.0	38.0	41.0	34.0	41.0
14	38.616035722074066	40.0	38.0	41.0	34.0	41.0
15	38.64913548805678	40.0	38.0	41.0	34.0	41.0
16	38.65411257370228	40.0	38.0	41.0	35.0	41.0
17	38.63991719166858	40.0	38.0	41.0	34.0	41.0
18	38.65480817519288	40.0	38.0	41.0	35.0	41.0
19	38.59888079744897	40.0	38.0	41.0	34.0	41.0
20	38.61230058437385	40.0	38.0	41.0	34.0	41.0
21	38.5204283011004	40.0	38.0	41.0	34.0	41.0
22	38.45511013727148	40.0	38.0	41.0	34.0	41.0
23	38.36962152175744	40.0	38.0	41.0	34.0	41.0
24	38.28481523078491	40.0	37.0	41.0	34.0	41.0
25	38.16873805578695	40.0	37.0	41.0	33.0	41.0
26	38.155534985172494	40.0	37.0	41.0	33.0	41.0
27	38.13298442007088	40.0	37.0	41.0	33.0	41.0
28	38.12154132887408	40.0	37.0	41.0	33.0	41.0
29	38.09218263422295	40.0	37.0	41.0	33.0	41.0
30	38.06374813019755	40.0	37.0	41.0	33.0	41.0
31	37.96167089808894	40.0	37.0	41.0	33.0	41.0
32	37.84860251547448	40.0	36.0	41.0	33.0	41.0
33	37.8310505770646	40.0	36.0	41.0	33.0	41.0
34	37.782822729168856	40.0	36.0	41.0	33.0	41.0
35	37.73503349398674	39.0	36.0	41.0	33.0	41.0
36	37.70282116648113	39.0	36.0	41.0	33.0	41.0
37	37.647130754673384	39.0	35.0	41.0	33.0	41.0
38	37.60647729643068	39.0	35.0	41.0	33.0	41.0
39	37.47408285959074	39.0	35.0	41.0	32.0	41.0
40	37.42399745772236	39.0	35.0	41.0	32.0	41.0
41	37.363853884846634	39.0	35.0	41.0	32.0	41.0
42	37.25294704780785	39.0	35.0	41.0	32.0	41.0
43	37.184941253065276	39.0	35.0	41.0	32.0	41.0
44	37.12080374010524	39.0	35.0	40.0	32.0	41.0
45	37.02788389950569	39.0	35.0	40.0	31.0	41.0
46	36.889772611615115	38.0	35.0	40.0	31.0	41.0
47	36.804298410393216	38.0	35.0	40.0	31.0	41.0
48	36.73980338795358	38.0	35.0	40.0	31.0	41.0
49	36.76943161318728	38.0	35.0	40.0	31.0	41.0
50	36.59508517393155	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	431.0
15	7175.0
16	13091.0
17	579.0
18	3.0
19	11.0
20	41.0
21	146.0
22	360.0
23	688.0
24	1280.0
25	2492.0
26	4665.0
27	9461.0
28	19386.0
29	38607.0
30	72626.0
31	131072.0
32	217457.0
33	345890.0
34	501381.0
35	564622.0
36	783319.0
37	1043382.0
38	1305540.0
39	1870778.0
40	2103735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.54661697252147	14.433448794845798	30.01993423263273
2	28.026555225856946	27.29391104731783	29.18078679421537	15.498746932609858
3	27.091216291347603	20.02628614635557	26.959984713805955	25.922512848490868
4	22.612978815189404	23.7178422612149	30.48349890979652	23.185680013799175
5	22.245273710390574	24.86534684203894	27.692142070878774	25.197237376691717
6	20.816574412154782	31.128769699258296	27.01766199343875	21.03699389514817
7	28.649354778999097	27.546339041720707	23.37479908933406	20.42950708994613
8	24.898406445250675	26.81100628099269	25.04257198402208	23.24801528973456
9	24.484973314991965	27.64003004573577	27.295902589679983	20.579094049592282
10	23.47176507412244	24.566626551840233	25.747644364969613	26.213964009067713
11	22.926757378470832	28.689517549969178	26.10441811502507	22.27930695653492
12	25.78514068199926	24.359273799567415	27.755716528728385	22.099868989704945
13	21.35887139736902	25.58538898307532	27.468182068130442	25.58755755142522
14	20.28431258762095	27.326030199969665	29.834322484480076	22.555334727929313
15	20.900197063116735	28.259775900589286	26.917797208101018	23.922229828192958
16	22.800891901182766	32.141468990413046	23.95916187488666	21.098477233517528
17	24.334169301680053	30.399621783977178	22.905060630847597	22.36114828349517
18	24.400808521942537	26.80149113415129	23.292349235540932	25.505351108365243
19	22.971511760998098	27.901165505910953	25.70849949342907	23.418823239661876
20	25.3945439041599	26.765864654117223	25.570319645868363	22.269271795854518
21	25.251794573290475	28.537750957848896	23.638567811076978	22.57188665778365
22	24.330175152831515	29.907666564028474	23.65171199066719	22.110446292472822
23	24.588544581948153	29.113019033280995	24.136608299354485	22.161828085416367
24	23.97069069233868	29.5641586989298	23.77967965155975	22.685470957171773
25	22.476710108139642	31.64998833025447	23.98658792913622	21.886713632469668
26	24.171034132997622	29.70229419612252	24.35660516870145	21.770066502178402
27	23.25814386907295	29.92627403366226	24.185850233572463	22.62973186369233
28	22.93096441333933	29.1745365731107	24.75037499879216	23.144124014757818
29	23.190701682015224	29.607970588242022	24.901567664637504	22.299760065105247
30	24.695831458877127	28.67413086930284	23.626945236478555	23.00309243534148
31	22.416391432883195	30.516532696753018	25.059528655043184	22.007547215320603
32	22.982771468062214	29.11036707918717	26.19127292274634	21.715588530004275
33	21.54306748720007	29.459594080136547	25.959840425813173	23.037498006850207
34	22.820603228024773	29.521289677528596	25.787343013813175	21.87076408063346
35	22.98382192960682	29.178228182737918	24.86796726830579	22.969982619349473
36	21.427003019576638	30.666509820062842	25.081209445439846	22.825277714920674
37	21.471618231077965	30.96749517835996	25.43771201190132	22.123174578660752
38	22.824702157904206	29.526831148645698	25.521563066766173	22.126903626683923
39	22.78203572480873	30.041843913377235	24.4044039240711	22.771716437742935
40	22.133309943589513	31.380524115306997	24.802467075172984	21.683698865930506
41	22.89529435332596	29.842589683044284	25.707968403783752	21.554147559846
42	21.325244591385744	30.85174578179845	25.672274205842587	22.150735420973223
43	22.423494720629066	30.18466398475974	25.166360983320835	22.225480311290355
44	22.893755238383832	29.67862967271519	25.195974924119845	22.231640164781137
45	22.924751529479583	29.50770501842035	25.40113232736352	22.166411124736545
46	23.210791073638738	30.054591353240124	24.42803533883079	22.30658223429035
47	23.113069354030443	29.09442204885446	25.047028955281625	22.74547964183347
48	22.245136633346764	29.50619093606756	25.65910203145853	22.589570399127144
49	22.348438543757858	29.894777071525375	24.131222019658267	23.625562365058496
50	21.32175806100852	30.960279987380506	25.783128470743904	21.93483348086707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117185.0
1	97689.0
2	67476.0
3	49930.0
4	41395.5
5	36257.0
6	29588.5
7	24838.5
8	22817.0
9	23014.0
10	23275.0
11	23886.5
12	25324.5
13	28285.5
14	33051.0
15	38089.0
16	41763.5
17	49540.0
18	57106.5
19	63040.0
20	70531.5
21	82251.0
22	94288.0
23	114317.5
24	131809.5
25	139593.0
26	152450.0
27	179614.5
28	199038.5
29	210272.5
30	223352.0
31	238581.0
32	263408.5
33	310197.5
34	359180.5
35	370812.5
36	375626.5
37	388978.5
38	403823.5
39	438490.0
40	477678.0
41	505447.5
42	541264.5
43	581532.5
44	595976.5
45	572468.5
46	570547.0
47	572026.0
48	568722.0
49	584334.5
50	609793.0
51	605457.5
52	573851.5
53	560603.0
54	554650.0
55	521529.0
56	474833.5
57	444442.5
58	409375.5
59	367303.5
60	333443.0
61	302650.5
62	281596.5
63	266602.5
64	244292.0
65	214160.0
66	185000.5
67	166768.0
68	150915.0
69	139373.5
70	120863.5
71	101204.5
72	92093.5
73	83554.0
74	73216.0
75	65373.0
76	57424.5
77	45833.5
78	34028.5
79	27725.5
80	22632.5
81	17890.5
82	14237.0
83	10827.5
84	8230.5
85	6721.0
86	5328.5
87	3906.0
88	2625.0
89	2108.0
90	1598.0
91	1005.5
92	704.0
93	548.5
94	307.5
95	96.5
96	89.0
97	88.5
98	44.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	66303.0
25	59428.0
26	56994.0
27	58801.0
28	55157.0
29	56797.0
30	61163.0
31	58832.0
32	54347.0
33	52967.0
34	53834.0
35	55739.0
36	59704.0
37	56696.0
38	62290.0
39	63052.0
40	64268.0
41	71924.0
42	75045.0
43	83549.0
44	123095.0
45	103152.0
46	109902.0
47	182343.0
48	494986.0
49	1574189.0
50	5223664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.116314111405792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.16161817713984	7.688222721101991
2	13.622314814596875	3.0285986080703085
3	5.625234413397692	1.8759561806885472
4	2.8079632474086553	1.2485680588591068
5	1.6775589612529513	0.9324136176845713
6	1.0591681060248022	0.7064427218012672
7	0.743937543100564	0.5788890385861348
8	0.550910383878838	0.48992742995458466
9	0.42160224983142414	0.4217996735281341
>10	2.731880596519191	6.013557357607026
>50	0.42934440766155535	3.397350921446503
>100	0.5862564453132285	15.824657933287964
>500	0.4746444742748543	39.05887859034884
>1k	0.10557605601774392	15.80512021146488
>5k	7.960494327445347E-4	0.6190191540433521
>10k+	0.001194074149116802	2.3105977815268886
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	29172	0.322762632159581	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	25405	0.28108407616941433	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22331	0.24707295827353634	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	21996	0.2433664766550851	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	21651	0.23954935379429204	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	15532	0.1718479776053274	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	14346	0.15872592626358661	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	13940	0.1542338918245084	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	11735	0.12983749788813528	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11698	0.12942812529147052	No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA	10717	0.11857421941773719	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	10365	0.11467964768730482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07603266173730427	0.0	0.0	0.0	0.0
2	0.14152121307943233	0.0	0.0	0.0	0.0
3	0.1849921571955366	0.0	0.0	0.0	0.0
4	0.24365414388517387	0.0	0.0	0.0	0.0
5	0.30389830034029924	0.0	0.0	0.0	0.0
6	0.4072593489360351	0.0	0.0	0.0	0.0
7	0.5027980616982036	0.0	0.0	0.0	0.0
8	0.6128086489586834	0.0	0.0	0.0	0.0
9	0.7132598328808291	0.0	0.0	0.0	0.0
10	0.8134565419455886	0.0	0.0	0.0	0.0
11	0.9346972153037638	0.0	0.0	0.0	0.0
12	1.0446524819430727	0.0	0.0	0.0	0.0
13	1.1585244485612822	0.0	0.0	0.0	0.0
14	1.2792893645773875	0.0	0.0	0.0	0.0
15	1.4107975452248844	0.0	0.0	0.0	0.0
16	1.535445968847188	0.0	0.0	0.0	0.0
17	1.6937072018929389	0.0	0.0	0.0	0.0
18	1.807014898175205	0.0	0.0	0.0	0.0
19	1.9531055945633549	0.0	0.0	0.0	0.0
20	2.093863383070629	0.0	0.0	0.0	0.0
21	2.220558669676256	0.0	0.0	0.0	0.0
22	2.339022247851651	0.0	0.0	0.0	0.0
23	2.444065043330983	0.0	0.0	0.0	0.0
24	2.560769425753143	0.0	0.0	0.0	0.0
25	2.679564927655564	0.0	0.0	1.1064124234182811E-5	0.0
26	2.806935125839477	0.0	0.0	1.1064124234182811E-5	0.0
27	2.914035848426366	0.0	0.0	1.1064124234182811E-5	0.0
28	3.0189901309118246	0.0	0.0	1.1064124234182811E-5	0.0
29	3.1489493341665358	0.0	0.0	1.1064124234182811E-5	0.0
30	3.2571011485556727	0.0	0.0	1.1064124234182811E-5	0.0
31	3.3706301273226225	0.0	0.0	1.1064124234182811E-5	0.0
32	3.4742456507757447	0.0	0.0	1.1064124234182811E-5	0.0
33	3.579863780715254	0.0	0.0	1.1064124234182811E-5	0.0
34	3.690228419951227	0.0	0.0	1.1064124234182811E-5	0.0
35	3.7988891840551364	0.0	0.0	1.1064124234182811E-5	0.0
36	3.89835566092044	0.0	0.0	1.1064124234182811E-5	0.0
37	3.9951999403422422	0.0	0.0	1.1064124234182811E-5	0.0
38	4.0930178626966525	0.0	0.0	1.1064124234182811E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGGTA	75	0.0	71.51009	44
CGATCGT	85	0.0	54.870956	43
CGTACGG	30	8.9647983E-7	51.149807	42
CGTTATA	35	5.3722033E-8	49.907883	41
GGTACGT	65	0.0	47.21521	42
TTCGTAC	115	0.0	46.702	42
CGCTAGT	55	1.8189894E-12	44.70286	30
AACGACA	1320	0.0	44.06304	43
TAATCCG	70	0.0	43.908817	37
TAAGCCA	5335	0.0	43.76391	44
AATGCGC	145	0.0	43.625942	36
GCGTACG	35	3.0703359E-6	42.778187	41
CGGTATA	50	4.7293724E-11	41.873985	20
GCGTTAG	150	0.0	41.799946	35
TCGTACA	125	0.0	41.701927	43
GCGACTA	30	6.197747E-5	41.5899	41
CGGGTAT	105	0.0	41.266136	10
ATTCGCG	295	0.0	41.266136	11
CTAAGCG	150	0.0	41.266136	6
TCTAGCG	45	9.0221874E-10	41.266136	4
>>END_MODULE