FastQCFastQC Report
Tue 24 May 2016
ERR840885.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840885.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183065
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA254790.6090988306421249No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA247680.5921017244532418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA242790.5804117315891577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA229270.5480909333228148No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC216990.5187344686252784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA199510.4769469276714562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA198160.47371962902799747No Hit
AAAAAAAAAAAAAAAAAAAAAAAA171330.4095800567287384No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT168110.4018823518161922No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA131050.3132870275742787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124990.2988000425525303No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA98740.23604701337416467No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT75030.17936608682867705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71290.1704252742905023No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG62040.14831230210384014No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG52580.12569730568375104No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50580.12091612250825651No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC49090.11735414104251309No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC46580.11135375615726746No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG46410.11094735558735043No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT46270.11061267276506581No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA44000.10518602986087952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA451.8189894E-1275.0825944
TCGACAT850.075.0825944
GCAATTA1200.065.69726644
GTCGTAC950.063.22744444
TTGGCGT1050.060.78114744
TAAGCCA34500.057.2368744
TGGCGTA1000.056.31194344
TCTCGTG750.055.09165243
GCGTAGA750.055.06056644
CGCCTAC850.053.21843341
ACGAACG700.053.21843341
TAGACGA507.4578566E-1153.1240943
CGTTCGA451.7516868E-952.4682443
ATCGACC850.052.2229940
CGTAATC850.052.0824443
TCGATCC900.051.6695542
ACATACG404.0996383E-851.64842643
TTGCGAC354.9503797E-850.42728838
TTATACG309.887335E-750.42728438
CGAATTA451.2551027E-1050.42728438
Produced by FastQC (version 0.11.5)