##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840885.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4183065
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02127172300693	38.0	35.0	39.0	30.0	39.0
2	36.855075405235155	39.0	37.0	39.0	32.0	39.0
3	36.724112582520235	38.0	35.0	39.0	33.0	39.0
4	36.91754156342299	38.0	35.0	39.0	33.0	39.0
5	37.72175163426817	39.0	36.0	40.0	33.0	41.0
6	37.67335076074601	39.0	36.0	40.0	33.0	41.0
7	37.78551875239806	39.0	36.0	40.0	33.0	41.0
8	37.55332322113092	39.0	36.0	40.0	33.0	41.0
9	37.53672008443569	39.0	36.0	40.0	33.0	41.0
10	37.49714790470624	39.0	36.0	40.0	33.0	41.0
11	37.24285039797373	38.0	36.0	40.0	32.0	41.0
12	37.53389058023244	39.0	36.0	40.0	33.0	41.0
13	37.65670913552622	39.0	36.0	40.0	33.0	41.0
14	37.636474212090896	39.0	36.0	40.0	33.0	41.0
15	37.51886427774849	39.0	35.0	40.0	33.0	41.0
16	37.59118827940756	39.0	35.0	40.0	33.0	41.0
17	37.621315948951306	39.0	35.0	40.0	33.0	41.0
18	37.65175320010567	39.0	35.0	41.0	33.0	41.0
19	37.55308679162289	39.0	35.0	41.0	33.0	41.0
20	37.532248721929975	39.0	35.0	41.0	33.0	41.0
21	37.337502046944046	39.0	35.0	40.0	33.0	41.0
22	37.28209434947819	39.0	35.0	40.0	32.0	41.0
23	37.235088625206636	39.0	35.0	40.0	32.0	41.0
24	37.045168554636376	39.0	35.0	40.0	32.0	41.0
25	36.89782515865531	38.0	35.0	40.0	31.0	41.0
26	36.847660688973804	38.0	35.0	40.0	31.0	41.0
27	36.770623040991076	38.0	35.0	40.0	31.0	41.0
28	36.727171751495376	38.0	35.0	40.0	31.0	41.0
29	36.677312831719306	38.0	35.0	40.0	31.0	41.0
30	36.675937498621586	38.0	35.0	40.0	31.0	41.0
31	36.58822622706052	38.0	35.0	40.0	31.0	41.0
32	36.41916525500109	38.0	35.0	40.0	31.0	41.0
33	36.40674888841412	37.0	35.0	40.0	31.0	41.0
34	36.323214124853614	37.0	34.0	40.0	31.0	41.0
35	36.23858876159486	37.0	34.0	40.0	31.0	41.0
36	36.20492474801953	37.0	34.0	40.0	31.0	41.0
37	36.13688358830698	37.0	34.0	40.0	31.0	41.0
38	36.02860438292964	37.0	34.0	40.0	31.0	41.0
39	35.88325643087751	36.0	34.0	40.0	30.0	41.0
40	35.791063422205966	36.0	34.0	40.0	30.0	41.0
41	35.709844203398795	36.0	34.0	40.0	30.0	41.0
42	35.60790897812498	36.0	34.0	39.0	30.0	41.0
43	35.59338443700269	36.0	34.0	39.0	30.0	41.0
44	35.475136745262205	35.0	34.0	39.0	30.0	41.0
45	35.26350369423528	35.0	34.0	39.0	29.0	41.0
46	35.17257335277781	35.0	33.0	39.0	29.0	41.0
47	35.0066577170948	35.0	33.0	39.0	29.0	40.0
48	34.92716303312628	35.0	33.0	39.0	29.0	40.0
49	34.98464441141154	35.0	33.0	39.0	29.0	40.0
50	34.960452335886714	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	120.0
14	880.0
15	4496.0
16	524.0
17	11.0
18	1.0
19	8.0
20	20.0
21	65.0
22	190.0
23	474.0
24	1088.0
25	2541.0
26	5321.0
27	10910.0
28	21532.0
29	40360.0
30	71135.0
31	118868.0
32	186400.0
33	281468.0
34	386209.0
35	414613.0
36	564496.0
37	569030.0
38	559545.0
39	600076.0
40	342684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	60.41878383434156	15.081835926527559	24.49938023913088
2	27.84766672284557	30.998227376337685	24.618694665275342	16.535411235541403
3	23.064021238015666	25.18963965417702	28.33807268115604	23.408266426651274
4	23.17587701840636	27.691656715829183	27.07165678754693	22.06080947821753
5	19.12580846819258	29.734154262484562	25.66520003872758	25.47483723059527
6	18.625385931129447	36.61941662393484	24.59600795110762	20.159189493828087
7	28.797400948825803	31.611175059436082	20.157277020557892	19.434146971180226
8	22.213353127431677	29.84259149690478	26.00442498502892	21.93963039063462
9	25.015102562355594	31.197459279260542	24.89954136500389	18.887896793379973
10	23.242000781723448	28.497907634712828	22.50118035459645	25.758911228967275
11	20.921764304403588	32.51620522272544	26.84514823460788	19.716882238263093
12	23.736733710807744	27.667822517699342	27.499668305417202	21.09577546607571
13	20.68980520264447	31.295497440274055	25.680428107141534	22.334269249939936
14	21.57666686986695	29.533177227702655	24.617810146387875	24.27234575604252
15	20.64110885200206	30.277559636295397	29.445562046011712	19.635769465690828
16	21.495554097294686	35.64321854907825	19.606675966067943	23.254551387559122
17	25.098892797506135	35.184774800295955	19.81649818972452	19.899834212473387
18	23.783302434937063	30.70408898738126	21.742239243234327	23.77036933444735
19	23.59322649779528	30.93179283611419	22.44538394693843	23.029596719152103
20	24.78151785831681	30.731485166976846	22.655230076510886	21.83176689819546
21	21.939463049223477	33.30598496556951	22.718987154156103	22.035564831050916
22	21.086380441135866	34.51442901317574	22.54903999818315	21.850150547505237
23	24.342509619142902	32.62411652699635	22.954245272306313	20.079128581554436
24	22.254758173731464	32.636308544093865	23.776823931734267	21.332109350440405
25	21.208709435657696	35.516566577131556	21.862042988471465	21.41268099873928
26	21.65459771679653	35.036476656436086	23.525438246000544	19.783487380766847
27	20.951514179463558	33.576999541214256	24.18755758134122	21.28392869798097
28	22.60157359385497	31.33716628485989	22.716885041978564	23.34437507930658
29	21.63024901156224	31.981196898030905	25.49667653483059	20.89187755557626
30	22.4315196005043	31.37632298830921	24.767809729949644	21.424347681236846
31	22.07197732698182	31.765517129099706	24.34460970945272	21.81789583446575
32	21.095128605587675	31.607902353890953	25.898083453261854	21.398885587259517
33	21.613164629440547	29.937024206797997	25.87832507389798	22.57148608986348
34	21.26426940407663	32.03572007283385	24.612099713441935	22.08791080964759
35	21.38289533171464	30.341412891842033	25.377173239533075	22.898518536910252
36	20.542539062101987	31.36506621673088	26.595550449380433	21.496844271786696
37	20.41273888323598	33.43509494755948	24.225089984978883	21.927076184225662
38	20.94932605932025	32.12430929148531	24.845525443610963	22.080839205583477
39	21.453985098773142	31.290762152142413	26.353735044133042	20.9015177049514
40	20.512996146954535	32.573166020288966	24.87902629494312	22.034811537813383
41	22.722919202267565	31.185918669718866	24.561981591682493	21.529180536331076
42	20.449251438558207	32.91978181683578	24.95295877205429	21.67800797255172
43	21.682382675624133	30.10333638940294	25.594915049095864	22.619365885877063
44	21.43675940644851	31.51496892036611	25.442384532170912	21.60588714101447
45	20.931083006763323	31.037962976893795	26.7324279337798	21.29852608256308
46	21.93491685362074	31.726955579211747	23.423350496227894	22.914777070939618
47	22.090664577371598	31.21594743639354	24.136230956282457	22.5571570299524
48	21.599033065971355	29.50154917880742	24.95583840822943	23.9435793469918
49	21.066836482853137	29.565165429346894	24.177485258946113	25.190512828853855
50	20.473513536865582	32.4562564070717	25.50804309692308	21.562186959139645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194741.0
1	184925.0
2	104640.5
3	32790.5
4	30815.5
5	25899.5
6	17454.5
7	13356.0
8	12721.5
9	11053.5
10	9374.0
11	9274.0
12	10235.5
13	12149.5
14	14259.5
15	15854.5
16	17188.0
17	19472.0
18	22473.0
19	27942.0
20	33382.0
21	44736.0
22	57099.0
23	68779.0
24	82737.5
25	96721.5
26	105881.5
27	105572.0
28	103976.0
29	109580.0
30	115339.0
31	127157.5
32	146985.5
33	154766.5
34	155686.5
35	172783.0
36	194331.0
37	202692.0
38	212097.5
39	214394.5
40	213915.5
41	230758.0
42	252023.5
43	263182.5
44	264240.0
45	252542.0
46	247974.5
47	238915.5
48	228952.0
49	244939.5
50	272413.5
51	271016.0
52	244353.0
53	221513.0
54	211712.5
55	211888.5
56	207368.0
57	196704.5
58	184900.0
59	172015.5
60	160486.5
61	142095.0
62	120028.5
63	109175.0
64	101925.5
65	92199.5
66	79609.5
67	70731.5
68	70405.0
69	71822.0
70	59689.5
71	44112.5
72	36342.5
73	29263.0
74	23004.5
75	19834.0
76	16727.0
77	11853.0
78	7846.0
79	7698.5
80	8053.5
81	5924.0
82	4062.0
83	3466.5
84	2659.5
85	2182.0
86	1744.0
87	1162.5
88	670.5
89	548.0
90	471.5
91	455.5
92	452.5
93	343.5
94	162.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	52097.0
25	53977.0
26	55506.0
27	57438.0
28	57883.0
29	52105.0
30	45832.0
31	41400.0
32	36346.0
33	33848.0
34	31540.0
35	33760.0
36	32399.0
37	35564.0
38	35741.0
39	38441.0
40	41616.0
41	43643.0
42	41222.0
43	46870.0
44	59006.0
45	57293.0
46	59723.0
47	88521.0
48	223138.0
49	606002.0
50	2222154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.341561380042869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16212228950393	7.957480165004826
2	13.072988439119253	2.965362016057237
3	5.141388986066986	1.7493413629246533
4	2.614891126254719	1.186277928421893
5	1.5708068836917772	0.8907701343792075
6	1.031293930778155	0.7017890050071677
7	0.7458636603064972	0.5921480939156707
8	0.5236689899133956	0.4751379193542262
9	0.42521771391412005	0.43403695220146515
>10	3.011492984772345	6.9810059648567835
>50	0.5084458124015195	4.0623185620201845
>100	0.6744876427013508	18.716529047479398
>500	0.45211284290271203	36.41849898211331
>1k	0.06164218844601892	10.677617878509636
>5k	0.0012622973743212063	0.9789092936364773
>10k+	0.0023142118529222116	5.212776694117884
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	25479	0.6090988306421249	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	24768	0.5921017244532418	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	24279	0.5804117315891577	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	22927	0.5480909333228148	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	21699	0.5187344686252784	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19951	0.4769469276714562	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	19816	0.47371962902799747	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	17133	0.4095800567287384	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	16811	0.4018823518161922	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	13105	0.3132870275742787	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12499	0.2988000425525303	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	9874	0.23604701337416467	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	7503	0.17936608682867705	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7129	0.1704252742905023	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	6204	0.14831230210384014	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG	5258	0.12569730568375104	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5058	0.12091612250825651	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	4909	0.11735414104251309	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC	4658	0.11135375615726746	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	4641	0.11094735558735043	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	4627	0.11061267276506581	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	4400	0.10518602986087952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.12177673547984552	0.0	0.0	0.0	0.0
2	0.2277755664805591	0.0	0.0	0.0	0.0
3	0.33547171750857324	0.0	0.0	0.0	0.0
4	0.4557662862040155	0.0	0.0	0.0	0.0
5	0.5942532568822143	0.0	0.0	0.0	0.0
6	0.7866002560323591	0.0	0.0	0.0	0.0
7	0.9441163357490261	0.0	0.0	0.0	0.0
8	1.1178406264306195	0.0	0.0	0.0	0.0
9	1.291254140205806	0.0	0.0	0.0	0.0
10	1.4770987302372782	0.0	0.0	0.0	0.0
11	1.6750636196186288	0.0	0.0	0.0	0.0
12	1.8502461711687483	0.0	0.0	0.0	0.0
13	2.0444817376732134	0.0	0.0	0.0	0.0
14	2.1880845743491912	0.0	0.0	0.0	0.0
15	2.3351537688274027	0.0	0.0	0.0	0.0
16	2.460803262679399	0.0	0.0	0.0	0.0
17	2.6416037044607243	0.0	0.0	0.0	0.0
18	2.7607746951099252	0.0	0.0	0.0	0.0
19	2.9025128703474605	0.0	0.0	0.0	0.0
20	3.031867781160465	0.0	0.0	0.0	0.0
21	3.1678685365874064	0.0	0.0	0.0	0.0
22	3.309702335488452	0.0	0.0	0.0	0.0
23	3.4297817509409967	0.0	0.0	0.0	0.0
24	3.543358757274869	0.0	0.0	0.0	0.0
25	3.660521650990362	0.0	0.0	0.0	0.0
26	3.7854061555342793	0.0	0.0	0.0	0.0
27	3.894966968000736	0.0	0.0	0.0	0.0
28	4.004360439056051	9.562366350989048E-5	0.0	0.0	0.0
29	4.127499811740912	9.562366350989048E-5	0.0	0.0	0.0
30	4.243180538672003	9.562366350989048E-5	0.0	0.0	0.0
31	4.3563750503518355	9.562366350989048E-5	0.0	0.0	0.0
32	4.456038813645018	9.562366350989048E-5	0.0	0.0	0.0
33	4.550825770099197	9.562366350989048E-5	0.0	0.0	0.0
34	4.641381379443064	9.562366350989048E-5	0.0	0.0	0.0
35	4.727107993779681	9.562366350989048E-5	0.0	0.0	0.0
36	4.810109333706266	9.562366350989048E-5	0.0	0.0	0.0
37	4.885006568150387	9.562366350989048E-5	0.0	0.0	0.0
38	4.957154622268599	9.562366350989048E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	45	1.8189894E-12	75.08259	44
TCGACAT	85	0.0	75.08259	44
GCAATTA	120	0.0	65.697266	44
GTCGTAC	95	0.0	63.227444	44
TTGGCGT	105	0.0	60.781147	44
TAAGCCA	3450	0.0	57.23687	44
TGGCGTA	100	0.0	56.311943	44
TCTCGTG	75	0.0	55.091652	43
GCGTAGA	75	0.0	55.060566	44
CGCCTAC	85	0.0	53.218433	41
ACGAACG	70	0.0	53.218433	41
TAGACGA	50	7.4578566E-11	53.12409	43
CGTTCGA	45	1.7516868E-9	52.46824	43
ATCGACC	85	0.0	52.22299	40
CGTAATC	85	0.0	52.08244	43
TCGATCC	90	0.0	51.66955	42
ACATACG	40	4.0996383E-8	51.648426	43
TTGCGAC	35	4.9503797E-8	50.427288	38
TTATACG	30	9.887335E-7	50.427284	38
CGAATTA	45	1.2551027E-10	50.427284	38
>>END_MODULE