FastQCFastQC Report
Tue 24 May 2016
ERR840883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7263590
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA975551.3430686478724707No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA249120.3429709000645686No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG187940.25874257770606546No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC181820.2503169920108376No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT137190.18887354600135747No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA130230.1792915073675689No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC124050.1707833178910153No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT102180.14067423959777467No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA91720.1262736470533166No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG89270.12290065931584795No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG87770.12083556478270387No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG82190.11315341311940791No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA78710.10836239380251364No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT77990.10737114842660447No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG75050.10332356314164208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT456.548362E-1176.0664244
TAAGCCA112950.075.8012544
CATAGCG700.061.124844
GAACACA45450.053.1900144
AACTCGA2300.052.0889644
TTAAGCC114850.051.65093243
GTACGGG850.048.0117339
CGCGTAA900.047.50407838
TATCGAA1950.047.39407743
CGAGCTT1450.047.2136444
ATTAAGC111050.047.13219542
ACGTAAT2100.046.8623544
CTCGACG1650.046.6771244
CGAATTA1600.045.9572734
TACACCG1050.045.24197838
GCGTACT2650.045.0901534
ATACCGA1100.044.91834637
CGTAGTG1650.044.7974941
CGTTAAT1450.044.63250430
TATCGCG1050.044.46960436
Produced by FastQC (version 0.11.5)