##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840883.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7263590
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.98464574680013	39.0	37.0	39.0	33.0	39.0
2	37.43730827318172	39.0	37.0	39.0	35.0	39.0
3	37.57348542525115	39.0	37.0	39.0	35.0	39.0
4	37.854531712280014	39.0	37.0	39.0	34.0	41.0
5	38.589339431328035	40.0	38.0	41.0	34.0	41.0
6	38.573270793092675	40.0	38.0	41.0	34.0	41.0
7	38.586069147625345	40.0	38.0	41.0	34.0	41.0
8	38.46070014965052	40.0	38.0	41.0	34.0	41.0
9	38.387872112825754	40.0	38.0	41.0	34.0	41.0
10	38.364128481921476	40.0	38.0	41.0	34.0	41.0
11	38.33405547394608	40.0	38.0	41.0	34.0	41.0
12	38.42958812377901	40.0	38.0	41.0	34.0	41.0
13	38.507386154780214	40.0	38.0	41.0	34.0	41.0
14	38.47871796177923	40.0	38.0	41.0	34.0	41.0
15	38.48615849738215	40.0	38.0	41.0	34.0	41.0
16	38.49104065620444	40.0	38.0	41.0	34.0	41.0
17	38.509564003474864	40.0	38.0	41.0	34.0	41.0
18	38.49616663385461	40.0	38.0	41.0	34.0	41.0
19	38.46098568338797	40.0	38.0	41.0	34.0	41.0
20	38.45980004378001	40.0	38.0	41.0	34.0	41.0
21	38.36031824483486	40.0	38.0	41.0	34.0	41.0
22	38.30883901762076	40.0	37.0	41.0	34.0	41.0
23	38.225480375406654	40.0	37.0	41.0	33.0	41.0
24	38.13717459272894	40.0	37.0	41.0	33.0	41.0
25	38.018910320881425	40.0	37.0	41.0	33.0	41.0
26	37.977902327792776	40.0	37.0	41.0	33.0	41.0
27	37.90418358726032	40.0	37.0	41.0	33.0	41.0
28	37.878933568285525	40.0	37.0	41.0	33.0	41.0
29	37.824078443673066	40.0	36.0	41.0	33.0	41.0
30	37.81730567321551	40.0	36.0	41.0	33.0	41.0
31	37.68243096093221	39.0	36.0	41.0	33.0	41.0
32	37.585382673554015	39.0	36.0	41.0	32.0	41.0
33	37.595128218390755	39.0	36.0	41.0	33.0	41.0
34	37.54544639111422	39.0	35.0	41.0	32.0	41.0
35	37.47044663734973	39.0	35.0	41.0	32.0	41.0
36	37.42030002761375	39.0	35.0	41.0	32.0	41.0
37	37.328036587452026	39.0	35.0	41.0	32.0	41.0
38	37.259331974517046	39.0	35.0	41.0	32.0	41.0
39	37.17004641274687	39.0	35.0	41.0	32.0	41.0
40	37.08501890656192	39.0	35.0	40.0	32.0	41.0
41	37.002895823011116	39.0	35.0	40.0	31.0	41.0
42	36.88816352263514	38.0	35.0	40.0	31.0	41.0
43	36.83357841447455	38.0	35.0	40.0	31.0	41.0
44	36.70860300854095	38.0	35.0	40.0	31.0	41.0
45	36.60541544569658	38.0	35.0	40.0	31.0	41.0
46	36.41521853521534	38.0	34.0	40.0	31.0	41.0
47	36.35066147890717	38.0	34.0	40.0	31.0	41.0
48	36.276253974655035	37.0	34.0	40.0	31.0	41.0
49	36.468595422592124	38.0	34.0	40.0	31.0	41.0
50	36.58862445241987	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	21.0
14	614.0
15	7523.0
16	8407.0
17	211.0
18	1.0
19	3.0
20	23.0
21	61.0
22	180.0
23	401.0
24	922.0
25	1820.0
26	3938.0
27	8410.0
28	17467.0
29	36019.0
30	69605.0
31	124238.0
32	201524.0
33	308517.0
34	443776.0
35	493900.0
36	662403.0
37	859922.0
38	1058046.0
39	1431903.0
40	1523735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.85796555146973	14.644714252869449	33.497320195660826
2	26.016666689612162	24.218423672040963	30.378270249284444	19.38663938906243
3	24.716221592903782	19.344704202742722	27.828346588945685	28.11072761540781
4	21.013438258492013	20.96931407196717	31.50206715962768	26.515180509913144
5	20.32789570997262	23.488206245121216	30.406685950060506	25.777212094845662
6	22.961207887559734	26.61608102880256	26.464888574382638	23.957822509255063
7	29.11419835095318	24.660298832946243	22.182791154236405	24.042711661864175
8	24.217074476945974	24.287439131338637	25.7758353651569	25.719651026558495
9	24.037246044999787	23.196807639197697	28.603376567234662	24.162569748567858
10	25.922099127291048	22.000126659131368	25.292768451963838	26.785005761613746
11	25.93889522949396	24.336492010149254	25.395651461605073	24.328961298751718
12	26.68729925560226	21.375834814465023	26.924096762069443	25.012769167863276
13	24.35901530785741	21.6105259245084	27.08093380821329	26.94952495942089
14	22.93134662061047	24.089369031016343	25.2550873603824	27.724196987990783
15	22.176678474418296	24.785498630842326	27.871809945219926	25.166012949519452
16	25.405797959411252	29.525936898971445	22.145481779670934	22.922783361946365
17	25.4277843325408	27.139073102969746	20.65448352674091	26.778659037748554
18	24.992118222531833	24.088419087531097	22.12955301717195	28.78990967276512
19	23.731130198703397	24.679325237244942	24.82381301808059	26.76573154597107
20	27.45883234048177	22.213588597374027	25.040840686217148	25.286738375927055
21	25.73562109094814	24.75522434498643	24.90406259163857	24.605091972426855
22	26.466554417306043	26.465136385726616	23.187638619470537	23.8806705774968
23	27.8755133480827	24.47372442552512	23.95483500583045	23.69592722056173
24	26.36174398610054	25.821707998386472	22.493491510396375	25.323056505116615
25	26.543826605335298	27.049541649419297	23.59048122516448	22.816150520080924
26	26.735588759396755	25.797975367077587	25.428570711937944	22.037865161587714
27	25.937505874800188	26.5280583262789	23.09090388728689	24.443531911634025
28	25.71747658411231	25.570782124688314	24.95125015148258	23.760491139716798
29	28.08872602541639	25.920113134881866	23.24608443512397	22.745076404577777
30	27.146573372797654	25.75508986181575	22.772364752402964	24.325972012983634
31	24.805820635871616	26.090720206624667	24.891433493261307	24.21202566424241
32	23.786383799824698	25.56387592783736	26.564784379281964	24.08495589305598
33	25.566442909480475	23.921387791338024	26.309046751137416	24.20312254804409
34	24.82917358822001	24.608473025315202	27.13186171195937	23.430491674505415
35	23.971976322782208	25.428949320869414	25.47444826423678	25.124626092111598
36	24.384818376583183	24.531673982345403	27.43939707449431	23.644110566577105
37	23.44391662720428	25.679838167419344	24.382633305893645	26.493611899482733
38	23.659475473507317	27.176804921279864	24.541641645398588	24.622077959814227
39	24.26781993647177	26.906418595347382	24.710983760569867	24.114777707610976
40	23.938763853376603	28.547610006809037	22.98519146053748	24.52843467927688
41	24.70942258401209	25.931903785633825	25.008576573741266	24.35009705661282
42	24.283816404567325	26.732040705792308	25.671600451342858	23.312542438297505
43	25.037439016980645	25.69610442188082	26.18913149350905	23.077325067629488
44	24.252782393517816	25.86392194584982	25.712118915445682	24.17117674518668
45	25.07957820619585	25.287230787639135	25.81424015842564	23.818950847739377
46	23.585002611482327	27.489087386117582	24.415099506257476	24.510810496142614
47	25.610044837252676	25.915335184597115	24.60962373574743	23.86499624240278
48	24.058387370053875	25.720810267739118	24.687786734925126	25.533015627281873
49	23.58279039238318	25.236398732975786	23.926948557604202	27.25386231703683
50	22.503353289682217	31.72645044605255	23.99935063506005	21.770845629205187
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30935.0
1	24067.0
2	16025.0
3	12489.0
4	9580.5
5	8429.5
6	6725.0
7	5662.0
8	5931.0
9	6186.0
10	6167.5
11	6717.5
12	7429.0
13	8572.0
14	10278.5
15	12718.0
16	14662.5
17	17027.5
18	19624.0
19	21675.0
20	23674.5
21	28784.0
22	33354.5
23	42510.5
24	52605.5
25	60597.5
26	70892.5
27	83907.0
28	93337.0
29	108499.5
30	127421.0
31	151136.0
32	177629.5
33	191366.5
34	213532.0
35	251052.0
36	273782.0
37	280172.5
38	292863.0
39	303526.5
40	311879.5
41	337413.5
42	373841.0
43	439337.0
44	497795.5
45	465422.5
46	436796.0
47	432948.5
48	425074.5
49	448818.0
50	494587.5
51	502491.5
52	490317.5
53	485631.5
54	464901.0
55	456917.0
56	448024.0
57	434970.0
58	421501.0
59	411383.5
60	393283.0
61	359046.5
62	332008.0
63	314976.5
64	289674.5
65	258746.5
66	239500.5
67	225366.5
68	199169.5
69	172752.0
70	149051.0
71	125551.0
72	113682.5
73	105973.5
74	92552.0
75	78143.5
76	67176.5
77	53864.5
78	43135.0
79	39999.0
80	36648.0
81	26427.5
82	18067.5
83	15536.0
84	13111.5
85	10136.5
86	7085.0
87	4585.5
88	2787.5
89	1894.0
90	1333.5
91	988.0
92	905.5
93	835.5
94	506.5
95	200.5
96	148.5
97	148.0
98	74.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	45098.0
25	42080.0
26	48510.0
27	47880.0
28	47001.0
29	42065.0
30	57418.0
31	47094.0
32	42418.0
33	43409.0
34	46614.0
35	50820.0
36	44944.0
37	50527.0
38	48738.0
39	51445.0
40	50988.0
41	51654.0
42	51889.0
43	59316.0
44	67417.0
45	76501.0
46	84718.0
47	183089.0
48	703524.0
49	1679630.0
50	3498803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.601944072780096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.3183396623004	5.790685928475223
2	13.89949736019805	2.3912539786435643
3	5.605630105972322	1.4465794999279884
4	2.9989915310905007	1.0318862970072653
5	1.7939394178974117	0.7715683271354608
6	1.1957736815809095	0.6171586999596803
7	0.8214152149767447	0.49460374178324185
8	0.6187959214633382	0.42582783271136476
9	0.48954448313692844	0.3789930838573708
>10	3.2489443483451117	5.573156003162212
>50	0.4892366209710212	2.977763133675848
>100	0.7366093309236974	15.890591886534908
>500	0.6408237483453368	40.703963764915166
>1k	0.13798176018513614	16.75852858528263
>5k	0.0031977232951364114	1.8756363133234157
>10k+	0.0012790893180545646	2.8718029236046827
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	97555	1.3430686478724707	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	24912	0.3429709000645686	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	18794	0.25874257770606546	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	18182	0.2503169920108376	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	13719	0.18887354600135747	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	13023	0.1792915073675689	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	12405	0.1707833178910153	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	10218	0.14067423959777467	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	9172	0.1262736470533166	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	8927	0.12290065931584795	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	8777	0.12083556478270387	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	8219	0.11315341311940791	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	7871	0.10836239380251364	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	7799	0.10737114842660447	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	7505	0.10332356314164208	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.027025203790412178	0.0	0.0	0.0	0.0
2	0.050402073905603154	0.0	0.0	0.0	0.0
3	0.071383434362347	0.0	0.0	0.0	0.0
4	0.09436105286779678	0.0	0.0	0.0	0.0
5	0.12334121281625202	0.0	0.0	0.0	0.0
6	0.16990221089020718	0.0	0.0	0.0	0.0
7	0.2091940762074952	0.0	0.0	0.0	0.0
8	0.2548601999837546	0.0	0.0	0.0	0.0
9	0.29639613469372583	0.0	0.0	0.0	0.0
10	0.33903345315470723	0.0	0.0	0.0	0.0
11	0.3879899608871095	0.0	0.0	0.0	0.0
12	0.43612043080625423	0.0	0.0	0.0	0.0
13	0.48580660527370073	0.0	0.0	0.0	0.0
14	0.535134830022069	0.0	0.0	0.0	0.0
15	0.5908923824169591	0.0	0.0	0.0	0.0
16	0.6449978591853339	0.0	0.0	0.0	0.0
17	0.7045138836305463	0.0	0.0	0.0	0.0
18	0.7580962031171914	0.0	0.0	0.0	0.0
19	0.8185208691569871	0.0	0.0	0.0	0.0
20	0.8781470319773005	0.0	0.0	0.0	0.0
21	0.933298823309135	0.0	0.0	0.0	0.0
22	0.9866333314518028	0.0	0.0	0.0	0.0
23	1.0359753234970586	0.0	0.0	0.0	0.0
24	1.0879331019509637	0.0	0.0	0.0	0.0
25	1.1410473333434294	0.0	0.0	0.0	0.0
26	1.1931703193599859	0.0	0.0	0.0	0.0
27	1.2444397329695096	0.0	0.0	0.0	0.0
28	1.2955990082039321	0.0	0.0	0.0	0.0
29	1.3598509827784884	0.0	0.0	0.0	0.0
30	1.413226792811819	0.0	0.0	0.0	0.0
31	1.4672634330957557	0.0	0.0	0.0	0.0
32	1.5247694321953744	0.0	0.0	0.0	0.0
33	1.581587066450612	0.0	0.0	0.0	0.0
34	1.6398089649883874	0.0	0.0	0.0	0.0
35	1.7001097253561943	0.0	0.0	0.0	0.0
36	1.7595156114263057	0.0	0.0	0.0	0.0
37	1.8160578997437906	0.0	0.0	0.0	0.0
38	1.8735638988434093	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAT	45	6.548362E-11	76.06642	44
TAAGCCA	11295	0.0	75.80125	44
CATAGCG	70	0.0	61.1248	44
GAACACA	4545	0.0	53.19001	44
AACTCGA	230	0.0	52.08896	44
TTAAGCC	11485	0.0	51.650932	43
GTACGGG	85	0.0	48.01173	39
CGCGTAA	90	0.0	47.504078	38
TATCGAA	195	0.0	47.394077	43
CGAGCTT	145	0.0	47.21364	44
ATTAAGC	11105	0.0	47.132195	42
ACGTAAT	210	0.0	46.86235	44
CTCGACG	165	0.0	46.67712	44
CGAATTA	160	0.0	45.95727	34
TACACCG	105	0.0	45.241978	38
GCGTACT	265	0.0	45.09015	34
ATACCGA	110	0.0	44.918346	37
CGTAGTG	165	0.0	44.79749	41
CGTTAAT	145	0.0	44.632504	30
TATCGCG	105	0.0	44.469604	36
>>END_MODULE