FastQCFastQC Report
Tue 24 May 2016
ERR840882.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840882.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8798468
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1611681.831773440558061No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA276410.31415696459883696No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA230730.2622388352154034No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT212840.24190574995556044No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG205410.23346109800024278No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG180430.20506979169555425No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA177160.20135323558601337No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG171110.19447703850261205No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC169040.19212435619473753No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG152290.17308695104647764No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG150300.17082519365871424No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG135060.1535039963775512No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC131400.1493441812824687No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT129290.14694603651453866No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG122700.13945609622038746No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT111350.12655612317962625No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG110840.12597647681391805No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG101600.11547464854108691No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT97390.11068972462024071No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC94960.10792788017186628No Hit
CTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACCCTGCGT93680.1064730814500888No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC92740.10540471363878348No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA91600.10410903352720041No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC91470.10396128053201989No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG88800.1009266613233122No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG88760.10088119886325665No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC88530.10061978971793725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA187400.0104.4498444
CTATGCG4600.066.189144
CATACCG2550.061.9961244
AGCGGTA1800.061.8047844
TACCGGC2100.061.3400844
CTATCAT41750.059.32323544
TTAAGCC182050.057.8939843
AACGCAC5200.057.425944
CGATAAG551.8189894E-1256.98158343
CGACGGG46050.056.45394544
GTATACC2100.052.97552544
CGATCGT850.049.5552640
ATTAAGC179450.049.49177642
GTAAGTA2850.049.30685844
TCGTGCG26800.049.11472743
GCGTACT700.048.75507742
GAACACA65700.048.6595744
AGGTCGA9350.048.60193643
AACGACA16350.047.9202943
TACGGTA205.2194874E-447.81089837
Produced by FastQC (version 0.11.5)