##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840882.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8798468
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.87054678155334	39.0	37.0	39.0	33.0	39.0
2	37.4133652585882	39.0	37.0	39.0	35.0	39.0
3	37.50841851104079	39.0	37.0	39.0	34.0	39.0
4	37.90130293137396	39.0	37.0	40.0	34.0	41.0
5	38.458522665536776	40.0	38.0	41.0	34.0	41.0
6	38.40398487554879	40.0	38.0	41.0	34.0	41.0
7	38.38011947079878	40.0	38.0	41.0	34.0	41.0
8	38.22871038458059	40.0	37.0	41.0	34.0	41.0
9	38.1413502896186	40.0	37.0	41.0	34.0	41.0
10	38.08189653016866	39.0	37.0	41.0	34.0	41.0
11	38.064555443061224	39.0	37.0	41.0	33.0	41.0
12	38.23582014505253	40.0	37.0	41.0	34.0	41.0
13	38.33705367798121	40.0	38.0	41.0	34.0	41.0
14	38.36045650220016	40.0	38.0	41.0	34.0	41.0
15	38.34300403206558	40.0	38.0	41.0	34.0	41.0
16	38.369983956297844	40.0	38.0	41.0	34.0	41.0
17	38.367388277140975	40.0	38.0	41.0	34.0	41.0
18	38.32881542559455	40.0	37.0	41.0	34.0	41.0
19	38.303747311463766	40.0	37.0	41.0	34.0	41.0
20	38.297149458292054	40.0	37.0	41.0	34.0	41.0
21	38.12352514096772	40.0	37.0	41.0	33.0	41.0
22	38.09639303115042	40.0	37.0	41.0	33.0	41.0
23	37.97509441416392	40.0	37.0	41.0	33.0	41.0
24	37.83780289932293	40.0	36.0	41.0	33.0	41.0
25	37.723819998941686	40.0	36.0	41.0	33.0	41.0
26	37.62767284891016	39.0	36.0	41.0	32.0	41.0
27	37.60092795156905	39.0	36.0	41.0	32.0	41.0
28	37.54354063836249	39.0	36.0	41.0	32.0	41.0
29	37.494851951758406	39.0	35.0	41.0	32.0	41.0
30	37.431562208808884	39.0	35.0	41.0	32.0	41.0
31	37.260441999526826	39.0	35.0	41.0	31.0	41.0
32	37.194854167817994	39.0	35.0	41.0	31.0	41.0
33	37.18116482362904	39.0	35.0	41.0	31.0	41.0
34	37.0740442117572	39.0	35.0	40.0	31.0	41.0
35	36.981510775490605	39.0	35.0	40.0	31.0	41.0
36	36.95130886580037	38.0	35.0	40.0	31.0	41.0
37	36.89663829303359	38.0	35.0	40.0	31.0	41.0
38	36.791803639441824	38.0	35.0	40.0	31.0	41.0
39	36.67445253009302	38.0	35.0	40.0	31.0	41.0
40	36.53627472215122	38.0	35.0	40.0	31.0	41.0
41	36.449470284588855	38.0	34.0	40.0	31.0	41.0
42	36.2976613954657	37.0	34.0	40.0	31.0	41.0
43	36.200524592958644	37.0	34.0	40.0	30.0	41.0
44	36.103733249968755	37.0	34.0	40.0	30.0	41.0
45	35.93305843505117	37.0	34.0	40.0	30.0	41.0
46	35.77104573425379	36.0	34.0	40.0	30.0	41.0
47	35.677221766815464	36.0	34.0	40.0	30.0	41.0
48	35.564559651948706	36.0	34.0	39.0	30.0	41.0
49	35.75277863385144	36.0	34.0	40.0	30.0	41.0
50	36.08270269427203	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	18.0
14	751.0
15	7030.0
16	6182.0
17	124.0
18	1.0
19	1.0
20	22.0
21	62.0
22	177.0
23	392.0
24	1017.0
25	2306.0
26	5207.0
27	11545.0
28	24466.0
29	50307.0
30	97106.0
31	171197.0
32	277563.0
33	427012.0
34	616858.0
35	693558.0
36	921408.0
37	1163050.0
38	1355947.0
39	1626196.0
40	1338965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.26869132217109	16.49947468127406	37.231833996554855
2	26.32134367028442	22.888768817480496	31.176541188761497	19.61334632347359
3	24.541795230715167	16.70863609437461	27.806374927998828	30.943193746911398
4	20.621442278360277	18.54481939355806	33.30748034771508	27.52625798036658
5	20.70031964655665	20.47659888062331	32.6118024183301	26.21127905448994
6	25.414810851161818	23.168340215592078	28.178337410558292	23.238511522687812
7	30.49807080050754	20.780685910319843	24.151329526913095	24.56991376225952
8	25.74576619475118	18.634641849012805	28.43825766031086	27.181334295925154
9	24.815115540569106	20.96994613153108	29.368590077272543	24.84634825062727
10	27.817035874881856	19.416721183733348	26.136311457858348	26.62993148352645
11	26.752805147441578	22.861707288132436	25.84022582113159	24.5452617432944
12	27.02346590338227	19.61216429951214	27.290887459044004	26.07348233806158
13	24.901585139594758	19.095813043816264	26.86996190700472	29.132639909584256
14	24.162967916687315	21.647336786358718	24.633629400027367	29.556065896926604
15	23.165669296063815	22.98538791071355	27.72206479582582	26.126877997396818
16	27.053402933328847	25.02214021804705	22.11021282341426	25.81424402520984
17	27.29797960281267	22.00710396400828	21.97695098737644	28.717965445802612
18	25.33186459279047	21.135975035653935	22.562189235671482	30.969971135884112
19	25.66351323889568	23.666165518815323	23.589595370466768	27.08072587182223
20	29.88111112070874	19.443850906771498	24.48339870077382	26.19163927174594
21	27.746989589551273	22.479856720510888	23.825500075694997	25.947653614242842
22	28.114269438725014	24.753923069334345	22.70962399363162	24.422183498309025
23	30.090761255254893	23.090724430662245	22.90685151096759	23.91166280311527
24	28.484527079032397	24.201565545274473	21.25738253523227	26.05652484046086
25	27.581692204750908	24.867144006756707	23.693551235717077	23.857612552775308
26	28.04333602407342	23.43256316809329	25.513417543233203	23.01068326460009
27	28.18995408235242	25.475183855357898	22.083629755528094	24.251232306761587
28	27.30447790247205	21.94457871146681	25.30351211688465	25.447431269176484
29	31.236000468181548	22.486084177483328	23.011016793709928	23.266898560625197
30	27.829545748114203	24.01010647081044	22.529624928881955	25.6307228521934
31	25.56594806075131	23.261231942294806	26.53764629322276	24.635173703731127
32	25.237739827395327	21.273528788396824	28.271627355611656	25.21710402859619
33	27.967499676545426	21.13303931639346	26.227791903049884	24.67166910401123
34	25.841003847105505	21.17954925506879	27.681371265878436	25.29807563194727
35	23.907033185781195	22.297123281256695	25.315515989132354	28.48032754382976
36	25.21579542091663	21.991676175230907	27.4838580503248	25.308670353527663
37	23.248166004367604	22.541247965427914	25.942665017820154	28.267921012384328
38	24.591011348527747	24.363067011060227	24.622665669540645	26.423255970871377
39	24.860069468345188	24.565552146503805	24.582670450224278	25.991707934926726
40	23.94071431870054	26.598345167964606	23.778327176983513	25.682613336351345
41	27.346688571347173	22.27343905190703	24.661028220197444	25.718844156548354
42	24.952191833795357	25.023463439574584	25.432473400371226	24.59187132625883
43	24.15403320876339	23.450184442499026	26.505212691874075	25.890569656863505
44	23.80951284932327	24.67101749234467	25.783902071356053	25.73556758697601
45	24.40228789845192	24.475804022714705	26.43070318104407	24.691204897789316
46	24.050422863585965	27.141086677141345	23.263516811439846	25.544973647832848
47	26.640333819592758	23.009943953393744	23.739149293896457	26.610572933117037
48	23.901945321178825	23.839520265060965	23.78296134115955	28.475573072600664
49	24.604317102001318	24.099782269820892	22.663268253999718	28.632632374178073
50	22.446797241716343	31.4879031026467	23.679947075058667	22.38535258057829
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19372.0
1	15194.5
2	10495.0
3	8625.0
4	6882.0
5	5656.5
6	4469.0
7	4317.0
8	4914.0
9	6112.0
10	7189.0
11	7966.0
12	8351.5
13	8322.0
14	8978.0
15	10307.5
16	11245.5
17	13738.5
18	16705.0
19	18427.0
20	19654.0
21	23292.5
22	26981.0
23	34131.0
24	40905.0
25	41811.5
26	45206.0
27	51713.5
28	57413.0
29	72332.5
30	89319.5
31	104887.5
32	123603.5
33	145710.5
34	170157.5
35	194354.0
36	214034.0
37	238060.5
38	267979.0
39	280855.5
40	293551.5
41	335608.0
42	402657.5
43	546121.0
44	668319.0
45	600793.5
46	528206.5
47	535796.0
48	560020.5
49	613495.5
50	675589.5
51	677191.0
52	641929.0
53	646710.0
54	649898.0
55	641408.0
56	643654.5
57	627707.5
58	584130.0
59	558102.0
60	547400.5
61	507252.5
62	460591.0
63	440358.5
64	416970.0
65	384424.5
66	339452.0
67	299967.0
68	261250.0
69	224758.0
70	203473.0
71	182911.5
72	167565.5
73	156891.5
74	144660.0
75	130386.5
76	115065.5
77	87047.5
78	64343.0
79	58728.5
80	52422.0
81	41068.5
82	31303.0
83	26460.0
84	22566.0
85	18258.0
86	13674.5
87	8230.5
88	4269.0
89	2877.0
90	1901.5
91	1166.5
92	850.5
93	672.5
94	463.5
95	198.0
96	121.5
97	122.0
98	74.0
99	25.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	67586.0
25	60405.0
26	65071.0
27	67279.0
28	71326.0
29	67034.0
30	94102.0
31	72400.0
32	71377.0
33	66711.0
34	65473.0
35	68460.0
36	64006.0
37	72082.0
38	67378.0
39	70593.0
40	70548.0
41	70855.0
42	84378.0
43	75373.0
44	81511.0
45	103025.0
46	128453.0
47	271286.0
48	1100663.0
49	2623527.0
50	3077566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.682294982191955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.37074382995861	5.849326711013893
2	13.619308694478772	2.364937110779926
3	5.62198354832107	1.4643515865466123
4	2.9392428606403236	1.0207749416152403
5	1.8474373830573128	0.8019998160416171
6	1.2032752660612893	0.6268314482831774
7	0.8642071433402527	0.5252310940938264
8	0.6447918663662969	0.4478618548728228
9	0.49119863733296154	0.3838258317757948
>10	3.4879850470518994	6.008001394464609
>50	0.511195160569309	3.1220815119038394
>100	0.6963803586251353	14.767647871990865
>500	0.54583100680348	35.12880832601811
>1k	0.1484346191163419	19.108299750597656
>5k	0.005628473211642594	3.4027060759842196
>10k+	0.0023561050653387603	4.977314674017831
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	161168	1.831773440558061	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	27641	0.31415696459883696	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	23073	0.2622388352154034	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	21284	0.24190574995556044	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	20541	0.23346109800024278	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	18043	0.20506979169555425	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	17716	0.20135323558601337	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	17111	0.19447703850261205	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	16904	0.19212435619473753	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	15229	0.17308695104647764	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	15030	0.17082519365871424	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	13506	0.1535039963775512	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	13140	0.1493441812824687	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	12929	0.14694603651453866	No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG	12270	0.13945609622038746	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	11135	0.12655612317962625	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	11084	0.12597647681391805	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	10160	0.11547464854108691	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	9739	0.11068972462024071	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	9496	0.10792788017186628	No Hit
CTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACCCTGCGT	9368	0.1064730814500888	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	9274	0.10540471363878348	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	9160	0.10410903352720041	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	9147	0.10396128053201989	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	8880	0.1009266613233122	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	8876	0.10088119886325665	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	8853	0.10061978971793725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05911256368722373	0.0	0.0	0.0	0.0
2	0.09431187338523024	0.0	0.0	0.0	0.0
3	0.12052098160725254	0.0	0.0	0.0	0.0
4	0.15001475256828803	0.0	0.0	0.0	0.0
5	0.18961255527666862	0.0	0.0	0.0	0.0
6	0.25755620182968214	0.0	0.0	0.0	0.0
7	0.32093087114711333	0.0	0.0	0.0	0.0
8	0.38981786374627947	0.0	0.0	0.0	0.0
9	0.45164680942182206	0.0	0.0	0.0	0.0
10	0.5152487910395309	0.0	0.0	0.0	0.0
11	0.5848518173845719	0.0	0.0	0.0	0.0
12	0.6506700939299888	0.0	0.0	0.0	0.0
13	0.7132832670414895	0.0	0.0	0.0	0.0
14	0.7865005589609464	0.0	0.0	0.0	0.0
15	0.867355544169735	0.0	0.0	0.0	0.0
16	0.9380724007861369	0.0	0.0	0.0	0.0
17	1.0364645299613524	0.0	0.0	0.0	0.0
18	1.1011235137753528	0.0	0.0	0.0	0.0
19	1.1839902128415993	0.0	0.0	0.0	0.0
20	1.2544456603126817	0.0	0.0	0.0	0.0
21	1.3290040948037771	0.0	0.0	0.0	0.0
22	1.3960271265406659	0.0	0.0	0.0	0.0
23	1.4583788905068473	0.0	0.0	0.0	0.0
24	1.5252541692485555	0.0	0.0	0.0	0.0
25	1.5925386101307637	0.0	0.0	0.0	0.0
26	1.6671766039269564	0.0	0.0	0.0	0.0
27	1.7317219315908179	0.0	0.0	0.0	0.0
28	1.792187003464694	0.0	0.0	0.0	0.0
29	1.8653474673090815	0.0	0.0	0.0	0.0
30	1.9317112933751648	0.0	0.0	0.0	0.0
31	1.9961543305039013	0.0	0.0	0.0	0.0
32	2.0568126178330135	0.0	0.0	0.0	0.0
33	2.1189143382688895	0.0	0.0	0.0	0.0
34	2.181482048920335	0.0	0.0	0.0	0.0
35	2.2473230566957794	0.0	0.0	0.0	0.0
36	2.3142324322825294	0.0	0.0	0.0	0.0
37	2.375333978597183	0.0	0.0	0.0	0.0
38	2.430707254944838	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	18740	0.0	104.44984	44
CTATGCG	460	0.0	66.1891	44
CATACCG	255	0.0	61.99612	44
AGCGGTA	180	0.0	61.80478	44
TACCGGC	210	0.0	61.34008	44
CTATCAT	4175	0.0	59.323235	44
TTAAGCC	18205	0.0	57.89398	43
AACGCAC	520	0.0	57.4259	44
CGATAAG	55	1.8189894E-12	56.981583	43
CGACGGG	4605	0.0	56.453945	44
GTATACC	210	0.0	52.975525	44
CGATCGT	85	0.0	49.55526	40
ATTAAGC	17945	0.0	49.491776	42
GTAAGTA	285	0.0	49.306858	44
TCGTGCG	2680	0.0	49.114727	43
GCGTACT	70	0.0	48.755077	42
GAACACA	6570	0.0	48.65957	44
AGGTCGA	935	0.0	48.601936	43
AACGACA	1635	0.0	47.92029	43
TACGGTA	20	5.2194874E-4	47.810898	37
>>END_MODULE