FastQCFastQC Report
Tue 24 May 2016
ERR840881.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840881.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6996937
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA446070.637521818475713No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC329080.4703200843454786No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA241130.3446222254109191No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT125810.17980724994379685No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA119690.17106056550173313No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC116530.16654430360027536No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG109690.15676859745914534No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT107210.15322418938458357No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101180.14460613265490313No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA99920.14280534468153708No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA84640.12096721751246296No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT82830.11838037129675456No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA79620.11379264955508389No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT76020.1086475410597523No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG73550.1051174249532331No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT71850.10268779038599318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA60050.057.76391644
CATCGCG200.005857196654.8270244
TTCGCAT1050.050.55368842
TACGGTA150.00807352450.55368442
CACGTTA2150.049.3021241
TTATGCG453.1232048E-949.1561743
GCGTCAC1900.048.5967940
TTAAGCC60400.047.24363743
GTTACGT1100.047.00204537
TAACGGT205.677442E-447.0020437
CGTCACG950.046.70727541
TAACGTT1500.046.29837444
ATGCGAA850.045.54174443
TTACCGC950.045.47844339
CTATCGT1850.045.41015239
CACGACG1250.044.4116335
CGATCGT1900.044.3734233
ACGCGCT351.3582394E-744.3577229
CGGATAC302.3924567E-644.35771629
CGTCATA302.5146346E-644.03782728
Produced by FastQC (version 0.11.5)