##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840881.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6996937
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.08585256663023	39.0	37.0	39.0	33.0	39.0
2	37.4989993478575	39.0	37.0	39.0	35.0	39.0
3	37.525228110528936	39.0	37.0	39.0	35.0	39.0
4	37.74911507706872	39.0	37.0	39.0	34.0	40.0
5	38.5311075403423	40.0	38.0	41.0	34.0	41.0
6	38.53269623550991	40.0	38.0	41.0	34.0	41.0
7	38.579869305669035	40.0	38.0	41.0	35.0	41.0
8	38.47147716207821	40.0	38.0	41.0	34.0	41.0
9	38.373062670136946	40.0	38.0	41.0	34.0	41.0
10	38.342733684753775	40.0	38.0	41.0	34.0	41.0
11	38.30174932259644	40.0	38.0	41.0	34.0	41.0
12	38.431246701235125	40.0	38.0	41.0	34.0	41.0
13	38.534756279783565	40.0	38.0	41.0	34.0	41.0
14	38.556800211292455	40.0	38.0	41.0	34.0	41.0
15	38.563250605229115	40.0	38.0	41.0	34.0	41.0
16	38.56042636942422	40.0	38.0	41.0	34.0	41.0
17	38.553192632719146	40.0	38.0	41.0	34.0	41.0
18	38.573172661123	40.0	38.0	41.0	34.0	41.0
19	38.54611010503596	40.0	38.0	41.0	34.0	41.0
20	38.50548833010787	40.0	38.0	41.0	34.0	41.0
21	38.42942404655066	40.0	38.0	41.0	34.0	41.0
22	38.33399386045637	40.0	37.0	41.0	34.0	41.0
23	38.270628133424665	40.0	37.0	41.0	33.0	41.0
24	38.17822198484851	40.0	37.0	41.0	33.0	41.0
25	38.054176885964644	40.0	37.0	41.0	33.0	41.0
26	37.98631379853922	40.0	37.0	41.0	33.0	41.0
27	37.973064052935534	40.0	37.0	41.0	33.0	41.0
28	37.91514359663458	40.0	36.0	41.0	33.0	41.0
29	37.88141821081158	40.0	36.0	41.0	33.0	41.0
30	37.834230987173434	40.0	36.0	41.0	33.0	41.0
31	37.705126873035134	39.0	36.0	41.0	33.0	41.0
32	37.58059598288526	39.0	35.0	41.0	33.0	41.0
33	37.549473480428595	39.0	35.0	41.0	33.0	41.0
34	37.51963477795904	39.0	35.0	41.0	33.0	41.0
35	37.43366553673979	39.0	35.0	41.0	32.0	41.0
36	37.40018998626659	39.0	35.0	41.0	32.0	41.0
37	37.34125943912905	39.0	35.0	41.0	32.0	41.0
38	37.23150198859957	39.0	35.0	41.0	32.0	41.0
39	37.09827398599572	39.0	35.0	40.0	32.0	41.0
40	37.0272211059694	38.0	35.0	40.0	32.0	41.0
41	36.944398357897605	38.0	35.0	40.0	31.0	41.0
42	36.815278675419414	38.0	35.0	40.0	31.0	41.0
43	36.75273173867878	38.0	35.0	40.0	31.0	41.0
44	36.63431489975469	38.0	35.0	40.0	31.0	41.0
45	36.517412505718646	38.0	35.0	40.0	31.0	41.0
46	36.360198869379055	37.0	34.0	40.0	31.0	41.0
47	36.24437373652184	37.0	34.0	40.0	31.0	41.0
48	36.1616190823163	37.0	34.0	40.0	31.0	41.0
49	36.29476495367371	37.0	34.0	40.0	31.0	41.0
50	36.19599046648992	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	109.0
14	985.0
15	11059.0
16	4067.0
17	26.0
18	0.0
19	2.0
20	22.0
21	79.0
22	195.0
23	340.0
24	734.0
25	1439.0
26	3208.0
27	7250.0
28	15683.0
29	32050.0
30	61496.0
31	109880.0
32	180458.0
33	284381.0
34	419584.0
35	490573.0
36	673690.0
37	865638.0
38	1067314.0
39	1429451.0
40	1337224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.2492003286581	14.110259960894316	32.640539710447584
2	25.666659568322537	26.70860120649936	29.360590212545862	18.26414901263224
3	27.538878797965456	19.378793892241706	26.84771922342591	26.234608086366933
4	22.89793376730418	21.447827813799094	31.728983696723294	23.92525472217343
5	19.819086551729708	25.482421808285537	29.72596437555462	24.972527264430138
6	21.51224171376704	29.34108167616773	27.22887171915368	21.91780489091155
7	27.18715346443737	27.897535735994193	22.853185615362836	22.062125184205602
8	24.346453312356537	25.483765253281543	26.6382418478257	23.531539586536223
9	22.87336587423897	26.46195042202038	27.986846244292323	22.677837459448327
10	24.356329062273964	24.051467091957523	25.390767417228428	26.201436428540088
11	24.560904292835563	26.049927275320616	25.935548655075785	23.453619776768033
12	25.110816347210214	23.775103305918005	26.781204404155705	24.332875942716075
13	21.731923554549656	23.836958943606323	28.69641387367072	25.7347036281733
14	20.85818408826605	25.71083604154218	27.51875570696149	25.91222416323028
15	20.414018305438507	27.69035936724884	27.399875116783246	24.495747210529405
16	23.150530010488875	32.50186474453036	22.33172886936098	22.01587637561979
17	23.331080442770887	30.544951312267067	21.866225178245852	24.25774306671619
18	22.421025085691067	26.227076219208488	23.8717170098859	27.480181685214543
19	22.999706871735444	26.05287142073739	25.043672681346134	25.90374902618103
20	24.98641905736753	24.28175357302774	25.328554480339037	25.403272889265686
21	24.27485055246317	26.118300050436353	24.743755731972435	24.86309366512804
22	24.912100823546073	26.885192763633576	24.183482000766908	24.019224412053447
23	25.498828987598433	25.673048078037574	26.319974011485307	22.508148922878682
24	24.952804348531366	26.8927532147281	24.406693957656042	23.74774847908449
25	24.365514422097352	28.125300611640892	24.301038841935757	23.208146124326
26	24.153615917683197	28.103415533562405	25.683503734684454	22.059464814069944
27	24.485814258464302	27.604292112949206	24.720966233323207	23.188927395263285
28	24.28390550518918	25.586859134188966	25.846559692329947	24.282675668291905
29	25.160218301807475	26.538467484795365	25.680544031780833	22.620770181616326
30	25.72652955402188	26.288220249678808	24.756944953423428	23.228305242875884
31	23.01555582355408	27.80658430631456	26.128166533818575	23.04969333631279
32	23.620493546684525	27.19515958169632	26.593861185584156	22.590485686035
33	23.657906338879716	26.15607060996111	26.31398488917188	23.87203816198729
34	24.0551004194752	27.200730760586588	26.633852639382642	22.110316180555568
35	22.73312635378176	26.40040733771939	26.62662262220285	24.239843686296002
36	23.287904675918902	26.821081189800573	26.523660375313952	23.367353758966576
37	23.052563340363857	27.586669725983498	25.25732204865898	24.103444884993664
38	23.073026056066183	28.37372158104458	24.833829779380252	23.71942258350898
39	23.65865312586696	27.956177666912268	25.982055352618953	22.40311385460182
40	22.219406465224907	30.03461847162685	24.988328801851143	22.7576462612971
41	23.06373876027473	28.282182063322065	26.227459387962842	22.426619788440362
42	22.705442636267374	29.12328572833075	26.19337806409188	21.977893571309995
43	23.468883954167765	27.265934085936088	26.330083221413688	22.93509873848246
44	22.901415562741434	28.640295610093645	25.95281479402264	22.505474033142285
45	23.896518827326297	28.01544014287695	26.257077216489964	21.830963813306788
46	22.527092239385958	29.842706809316976	24.84304539404625	22.787155557250813
47	24.47577742509007	28.063582608577125	24.789822829799217	22.670817136533593
48	22.80520986226423	28.095313683300738	25.19025465418511	23.90922180024992
49	22.043891561037025	28.039475131706006	24.559751286501815	25.356882020755155
50	21.94029171732081	31.35994055457126	24.764808912770405	21.934958815337527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43442.0
1	35780.0
2	26069.0
3	20756.0
4	16689.5
5	14259.5
6	11732.0
7	10603.5
8	10892.5
9	12027.5
10	12849.5
11	13638.0
12	14536.0
13	17178.5
14	20069.0
15	21834.0
16	23312.5
17	25982.0
18	27996.0
19	34793.5
20	42306.0
21	46110.0
22	50150.5
23	61794.0
24	73679.5
25	89820.5
26	105686.0
27	115698.5
28	129386.0
29	147941.0
30	168680.0
31	194494.0
32	218559.0
33	234489.0
34	249065.5
35	272847.0
36	297666.5
37	301240.5
38	303768.5
39	324956.0
40	349342.0
41	386116.5
42	428813.0
43	457792.5
44	476312.5
45	455250.5
46	436006.0
47	418461.5
48	403763.0
49	419842.0
50	450930.5
51	472655.5
52	474453.0
53	467052.5
54	444746.5
55	396207.0
56	356198.5
57	351276.5
58	345418.5
59	333363.5
60	318834.0
61	285988.0
62	251554.5
63	231018.5
64	208939.0
65	186849.5
66	176744.5
67	170653.0
68	149005.5
69	124284.0
70	109338.5
71	102726.0
72	95659.0
73	85195.5
74	79053.5
75	71533.0
76	58533.0
77	40198.0
78	25828.0
79	21014.0
80	16805.5
81	11437.0
82	7988.0
83	7592.5
84	6641.0
85	5616.0
86	5124.0
87	4138.0
88	2825.5
89	1882.5
90	1116.5
91	761.5
92	502.5
93	261.5
94	140.5
95	35.5
96	28.0
97	28.0
98	81.0
99	134.0
100	134.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	42366.0
25	40600.0
26	42670.0
27	41127.0
28	38659.0
29	37798.0
30	45554.0
31	48408.0
32	47940.0
33	40032.0
34	47467.0
35	45953.0
36	41196.0
37	43723.0
38	45813.0
39	45793.0
40	47904.0
41	49404.0
42	51306.0
43	57627.0
44	73632.0
45	70722.0
46	82028.0
47	148987.0
48	484324.0
49	1279334.0
50	3956570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.91220497181762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.41030889550038	6.096866950620575
2	13.621416645853216	2.4279371430874463
3	5.562407896443322	1.4871995792987929
4	2.8348015050041084	1.0105732826805474
5	1.6790487364549196	0.7482013248478813
6	1.0990588730428306	0.587702277159155
7	0.759544105168425	0.4738448925277762
8	0.5747471238403644	0.4097811339702378
9	0.4248233942902095	0.34075018500638765
>10	3.077083968980596	5.579776573913589
>50	0.4780421330072763	3.0270455357287607
>100	0.7084731703459082	15.748158521032963
>500	0.6246996142088975	41.18316303382629
>1k	0.1416927200992696	16.972688307799153
>5k	0.002407011100214093	1.4802726726132027
>10k+	0.0014442066601284558	2.4260385858872455
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	44607	0.637521818475713	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	32908	0.4703200843454786	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	24113	0.3446222254109191	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	12581	0.17980724994379685	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	11969	0.17106056550173313	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	11653	0.16654430360027536	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	10969	0.15676859745914534	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	10721	0.15322418938458357	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10118	0.14460613265490313	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	9992	0.14280534468153708	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	8464	0.12096721751246296	No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	8283	0.11838037129675456	No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA	7962	0.11379264955508389	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	7602	0.1086475410597523	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	7355	0.1051174249532331	No Hit
ACTGCATTTTCCGGCAAGCCAAGGGTTGTCTGCATCTCAAGAGTGGGGT	7185	0.10268779038599318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.043719130242276014	0.0	0.0	0.0	0.0
2	0.07278899324089956	0.0	0.0	0.0	0.0
3	0.10114425783739371	0.0	0.0	0.0	0.0
4	0.13543068917156179	0.0	0.0	0.0	0.0
5	0.1716608281595218	0.0	0.0	0.0	0.0
6	0.23065807223932416	0.0	0.0	0.0	0.0
7	0.28279517165868434	0.0	0.0	0.0	0.0
8	0.3417066639302312	0.0	0.0	0.0	0.0
9	0.3924574424494604	0.0	0.0	0.0	0.0
10	0.4443801623481818	0.0	0.0	0.0	0.0
11	0.5005475967555517	0.0	0.0	0.0	0.0
12	0.5586015709445433	0.0	0.0	0.0	0.0
13	0.6117819840310125	0.0	0.0	0.0	0.0
14	0.6727229357646067	0.0	0.0	0.0	0.0
15	0.7337925152105843	0.0	0.0	0.0	0.0
16	0.7931613504594939	0.0	0.0	0.0	0.0
17	0.86416384769507	0.0	0.0	0.0	0.0
18	0.9204313258787381	0.0	0.0	0.0	0.0
19	0.9932346110876802	0.0	0.0	0.0	0.0
20	1.0653375898625355	0.0	0.0	0.0	0.0
21	1.1283937528664327	0.0	0.0	0.0	0.0
22	1.1868193182245317	0.0	0.0	0.0	0.0
23	1.2369984180220575	0.0	0.0	0.0	0.0
24	1.2921939986025313	0.0	0.0	0.0	0.0
25	1.3472895354067072	0.0	0.0	0.0	0.0
26	1.4058151445411042	0.0	0.0	0.0	0.0
27	1.454636507374584	0.0	0.0	0.0	0.0
28	1.5033721183998083	0.0	0.0	0.0	0.0
29	1.572931126863083	0.0	0.0	0.0	0.0
30	1.6282267512198552	0.0	0.0	1.4291968042587779E-5	0.0
31	1.6862235575366764	0.0	0.0	1.4291968042587779E-5	0.0
32	1.7417907292862578	0.0	0.0	1.4291968042587779E-5	0.0
33	1.795385609445962	0.0	0.0	1.4291968042587779E-5	0.0
34	1.8596422977654365	0.0	0.0	1.4291968042587779E-5	0.0
35	1.9226841688012912	0.0	0.0	1.4291968042587779E-5	0.0
36	1.9851829450515275	0.0	0.0	1.4291968042587779E-5	0.0
37	2.0437371381220095	0.0	0.0	1.4291968042587779E-5	0.0
38	2.103977783421517	0.0	0.0	1.4291968042587779E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	6005	0.0	57.763916	44
CATCGCG	20	0.0058571966	54.82702	44
TTCGCAT	105	0.0	50.553688	42
TACGGTA	15	0.008073524	50.553684	42
CACGTTA	215	0.0	49.30212	41
TTATGCG	45	3.1232048E-9	49.15617	43
GCGTCAC	190	0.0	48.59679	40
TTAAGCC	6040	0.0	47.243637	43
GTTACGT	110	0.0	47.002045	37
TAACGGT	20	5.677442E-4	47.00204	37
CGTCACG	95	0.0	46.707275	41
TAACGTT	150	0.0	46.298374	44
ATGCGAA	85	0.0	45.541744	43
TTACCGC	95	0.0	45.478443	39
CTATCGT	185	0.0	45.410152	39
CACGACG	125	0.0	44.41163	35
CGATCGT	190	0.0	44.37342	33
ACGCGCT	35	1.3582394E-7	44.35772	29
CGGATAC	30	2.3924567E-6	44.357716	29
CGTCATA	30	2.5146346E-6	44.037827	28
>>END_MODULE