FastQCFastQC Report
Tue 24 May 2016
ERR840880.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840880.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356303
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT273642.0175432775714572No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC226691.6713816897846574No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA126260.930912930222819No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA121910.8988404508432113No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA109510.8074154521519159No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG61470.45321731206080057No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC58920.43441620345896165No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT39230.289242153117703No Hit
AGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCG29970.22096832344984857No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC29710.21905134767083756No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT28900.21307923082084165No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG28150.20754949299677136No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA26970.19884937215356746No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT25560.18845346504431532No Hit
CACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTA23550.17363376767580696No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC23310.17186425157210447No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG21910.1615420743005066No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC21400.1577818525801388No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA21280.15689709452828757No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC20800.1533580623208826No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG20230.14915546157458914No Hit
CTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAG19920.14686983660730676No Hit
TCTCGACACTGTCATCCGGCCAAGATGCAGATCTTTGTCAAGACCCTG19810.1460588083931098No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG18790.1385383649523742No Hit
ATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCAT17980.1325662481023783No Hit
AGCCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGA17780.1310916513492929No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG17700.13050181264805874No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC17030.1255619135252226No Hit
ATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAAT16970.12511953449929697No Hit
TTTTTCAGCTCACTTCAAGGGTACCTGAAGCGAATTGGCACCAAAGCA16820.12401358693448293No Hit
AGTCTCCCAGCTCCAGCTCGGCCTTTGGGTTTGCTGTGGTGTCCTTGTCT16530.12187542164250909No Hit
ATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCA16310.12025336521411514No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG16260.11988471602584379No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG15630.11523973625362474No Hit
CTCTTCGTTAAGTCGGCCTTCCCAACATGGCGCAGTCTATTAACATCAC15410.1136176798252308No Hit
CTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCAT15320.11295411128634236No Hit
CTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCCAA14770.1088989702153575No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT14610.10771929281288915No Hit
ATCGCTCTTCTACTATGAACCCCCCTCCCCATACCCAACCCCCTGGTCAA14520.10705572427400072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14440.10646588557276657No Hit
ACAGACGAGATCTCGATCGAAGGCGAGATGGCGGACGTGCTAGATCTTC14260.10513874849498968No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC14210.10477009930671834No Hit
TACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGAT14140.10425399044313845No Hit
AGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAAT13690.10093614774869628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAG600.082.9397844
AAATCAT303.159039E-882.9397844
TCCGATG1100.082.9397844
CGGTTCC600.082.9397844
GAGTACT451.8189894E-1282.9397844
TGTGCAC303.159039E-882.9397844
GTGGACT402.910383E-1182.9397844
AATGCGA150.00113472682.9397844
ATATCCG150.00113472682.9397844
TAGATCC251.0361491E-682.9397844
TGCGTCC550.082.9397844
AACCCGC1000.078.7927944
GAAGTTC1000.078.7927944
CGGATTA900.078.33201644
GCGTACA850.078.06097444
TGTTATG950.074.20927444
CCTCGAA1200.072.5723144
GTATCAT750.071.8811544
GTACTGG1100.071.62981444
TACTGTC359.196265E-871.0912444
Produced by FastQC (version 0.11.5)