##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840880.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1356303
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.27054942737722	38.0	35.0	39.0	31.0	39.0
2	36.864326039240495	39.0	37.0	39.0	32.0	39.0
3	36.916203090312415	38.0	37.0	39.0	33.0	39.0
4	37.16271880250947	39.0	37.0	39.0	33.0	40.0
5	37.71314226983203	39.0	37.0	40.0	33.0	41.0
6	37.63296991896353	39.0	36.0	40.0	33.0	41.0
7	37.58823655186194	39.0	36.0	40.0	33.0	41.0
8	37.50621579396344	39.0	36.0	40.0	33.0	41.0
9	37.38604648076426	38.0	36.0	40.0	32.0	41.0
10	37.32515743163585	39.0	36.0	40.0	32.0	41.0
11	37.27994776978301	38.0	36.0	40.0	32.0	41.0
12	37.526248928152484	39.0	36.0	40.0	33.0	41.0
13	37.780804879145734	39.0	37.0	40.0	33.0	41.0
14	37.858819894964476	39.0	37.0	40.0	33.0	41.0
15	37.87990589123522	39.0	37.0	40.0	33.0	41.0
16	37.960804481004615	39.0	37.0	41.0	33.0	41.0
17	37.93133613949095	39.0	37.0	41.0	33.0	41.0
18	38.0373028740628	40.0	37.0	41.0	33.0	41.0
19	37.97742761020214	40.0	37.0	41.0	33.0	41.0
20	37.88617956312122	39.0	37.0	41.0	33.0	41.0
21	37.829064007083964	39.0	37.0	41.0	33.0	41.0
22	37.76115440281412	39.0	36.0	41.0	33.0	41.0
23	37.73791107149361	39.0	36.0	41.0	33.0	41.0
24	37.4664687757824	39.0	36.0	41.0	32.0	41.0
25	37.452129913340556	39.0	36.0	41.0	32.0	41.0
26	37.339254032394074	39.0	35.0	40.0	32.0	41.0
27	37.26652665054894	39.0	35.0	40.0	32.0	41.0
28	37.2116077583088	39.0	35.0	40.0	32.0	41.0
29	37.21279243309094	39.0	35.0	40.0	32.0	41.0
30	37.185466095950105	39.0	35.0	40.0	32.0	41.0
31	37.084733544621145	39.0	35.0	40.0	31.0	41.0
32	36.969421242929315	39.0	35.0	40.0	31.0	41.0
33	36.91209026756206	38.0	35.0	40.0	31.0	41.0
34	36.876021410426226	38.0	35.0	40.0	31.0	41.0
35	36.798850737122066	38.0	35.0	40.0	31.0	41.0
36	36.757208675113716	38.0	35.0	40.0	31.0	41.0
37	36.67394412779065	38.0	35.0	40.0	31.0	41.0
38	36.549122271054124	38.0	35.0	40.0	31.0	41.0
39	36.417892744056694	38.0	34.0	40.0	31.0	41.0
40	36.356844024161745	38.0	34.0	40.0	31.0	41.0
41	36.23633506655787	37.0	34.0	40.0	31.0	41.0
42	36.15021329377479	37.0	34.0	40.0	31.0	41.0
43	36.114370578462626	37.0	34.0	40.0	31.0	41.0
44	35.9415032043689	37.0	34.0	40.0	30.0	41.0
45	35.74172764249417	36.0	34.0	39.0	30.0	41.0
46	35.6358455455807	36.0	34.0	39.0	30.0	41.0
47	35.49582651031421	36.0	34.0	39.0	30.0	41.0
48	35.46481307462376	36.0	34.0	39.0	30.0	40.0
49	35.51253629826483	36.0	34.0	39.0	30.0	41.0
50	35.317070565759586	36.0	33.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	31.0
14	401.0
15	3141.0
16	1355.0
17	24.0
18	0.0
19	0.0
20	3.0
21	19.0
22	49.0
23	113.0
24	258.0
25	599.0
26	1321.0
27	2562.0
28	5428.0
29	10415.0
30	19223.0
31	32924.0
32	50797.0
33	76319.0
34	103031.0
35	121748.0
36	157631.0
37	184540.0
38	210891.0
39	244333.0
40	129147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.04509021951585	15.970546404453872	35.98436337603028
2	25.304743851484513	24.66329426389236	30.218247692440407	19.813714192182722
3	25.403615563778892	19.451258310274326	25.36114717729003	29.783978948656753
4	21.716533842364132	21.90203811390228	27.800793775432187	28.580634268301402
5	19.23272307146707	25.13597625309389	28.184483850585007	27.446816824854032
6	19.81791679292901	30.030089146746707	23.900854012709548	26.25114004761473
7	23.45441984571294	26.76511074590265	21.707833721520927	28.072635686863478
8	23.91471522218855	25.292357238758594	26.236467809921532	24.556459729131323
9	21.874536884457235	27.768352646864308	27.65620956379216	22.700900904886296
10	27.932106616294444	22.10914522787312	22.898054490773816	27.060693665058622
11	22.573569475257372	26.49769262473061	24.16657634761554	26.762161552396478
12	22.622157438271536	25.27635786398762	25.422711591731346	26.678773106009494
13	21.200867357810164	24.95887718304833	24.31455213178766	29.525703327353842
14	17.420369932087446	28.60179473170818	24.168640783069858	29.809194553134517
15	21.801765534692468	28.022868046446845	27.653407829961303	22.52195858889938
16	20.960581816894898	33.469586073318425	20.931532260859115	24.638299848927563
17	20.67524734517287	35.15556627095863	18.346048043836813	25.823138340031687
18	20.19039993275839	29.965133159773295	20.93278566809924	28.91168123936908
19	21.472045700702573	26.336740389131336	23.406347991562356	28.784865918603735
20	24.21531177030501	22.20668980308972	27.000014008669154	26.57798441793611
21	22.114527506021883	31.11229570383609	22.177197868028014	24.59597892211401
22	24.23462898777043	26.90033126816058	20.8457844596672	28.01925528440179
23	25.47815642964736	25.576290843565193	22.901962172169494	26.043590554617957
24	20.84254034681041	28.738194931368582	25.29530643226477	25.123958289556242
25	22.32792622425602	26.870224922060025	20.92775703838497	29.874091815298982
26	23.3846250711136	27.374032424053674	25.834755605325704	23.40658689950702
27	23.404658924213695	28.09827226962902	21.701944027309946	26.79512477884734
28	20.577112760536668	25.127826249224057	24.231957133553962	30.063103856685313
29	24.849237141086075	30.012082536088226	22.857807830067156	22.280872492758544
30	26.57039375568941	25.540547345586955	24.155875346206738	23.7331835525169
31	23.244496650270776	27.38093192606697	23.123909522165693	26.250661901496564
32	21.030572458016806	29.41351084067173	24.649930080501907	24.905986620809554
33	22.676605011142133	25.736385121698145	25.348097422026893	26.23891244513283
34	22.73881967506831	28.749864025236917	25.04839101862694	23.462925281067832
35	21.26661138621256	28.185361229777488	21.859813182208306	28.68821420180165
36	19.99929884235612	28.695854935375277	27.05579828836005	24.249047933908553
37	21.14944779380792	30.829674994734624	21.483305470724513	26.53757174073294
38	22.977105827230268	27.83769693488053	22.437916429379058	26.747280808510144
39	21.6348070833822	29.832716245874447	23.897870629429207	24.634606041314143
40	19.29109424568951	30.237609664698283	24.724020373790054	25.747275715822155
41	21.25003840861992	30.10245712744492	21.98381375404144	26.663690709893718
42	20.927614583450058	29.705198610164146	23.14054288415937	26.22664392222643
43	20.445789308332092	26.978851464193	24.344096015623165	28.231263211851743
44	20.155158915032484	28.259565769924816	22.812799157361503	28.772476157681194
45	18.63699831088923	29.277324848497834	28.607037128297424	23.47863971231551
46	21.82295724938446	30.603947585825818	24.539461863568935	23.03363330122079
47	21.0342231649284	29.646338648877364	24.90887377139864	24.4105644147956
48	22.205233060551826	27.233069996080634	21.406910359873258	29.15478658349428
49	20.87922048976397	29.281755582974395	19.823891429930352	30.015132497331283
50	19.684853407945603	34.5119364592265	25.06596266377845	20.737247469049446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11121.0
1	8405.5
2	4815.0
3	3398.0
4	2722.0
5	2405.0
6	1930.0
7	1805.5
8	2015.5
9	2179.0
10	2288.5
11	2763.5
12	3300.0
13	3262.5
14	3574.5
15	4061.5
16	4231.0
17	4480.0
18	4767.5
19	5271.5
20	5773.5
21	7855.0
22	9587.5
23	9367.5
24	9228.0
25	9202.5
26	8902.0
27	10018.0
28	12142.0
29	14185.5
30	15815.5
31	23297.5
32	34647.0
33	34363.0
34	32693.0
35	50251.0
36	62919.0
37	56494.5
38	52552.5
39	57165.0
40	64446.5
41	68863.0
42	72166.5
43	84491.0
44	94753.5
45	94111.5
46	93412.0
47	90807.5
48	90340.0
49	111412.0
50	145028.5
51	136629.0
52	100793.5
53	80235.5
54	74086.5
55	76072.0
56	75231.5
57	74672.0
58	74798.5
59	68983.0
60	63205.5
61	58632.0
62	51376.5
63	45174.5
64	44109.5
65	43082.0
66	37327.0
67	33491.0
68	30171.0
69	27344.5
70	24288.5
71	18908.0
72	16694.5
73	15892.0
74	13313.0
75	12157.0
76	12211.5
77	11422.0
78	9522.5
79	6860.0
80	4797.5
81	3645.0
82	2947.5
83	2716.0
84	2637.0
85	2103.0
86	1481.5
87	1005.5
88	397.0
89	98.0
90	103.0
91	124.5
92	106.0
93	95.0
94	49.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11037.0
25	11133.0
26	10952.0
27	10266.0
28	11036.0
29	11104.0
30	10373.0
31	10370.0
32	10399.0
33	9401.0
34	11699.0
35	11990.0
36	11055.0
37	10902.0
38	10806.0
39	11084.0
40	11084.0
41	11475.0
42	11074.0
43	13582.0
44	15365.0
45	12553.0
46	15671.0
47	33047.0
48	121128.0
49	272947.0
50	664770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.948399647108767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14335261208608	6.878657047259941
2	12.923533229300007	2.5713694683553325
3	5.191037521065015	1.5492754752807465
4	2.7752916831376524	1.1043884320460207
5	1.6769869679937919	0.834166828029772
6	1.0858041560244522	0.6481208209573738
7	0.7902403082715183	0.5503138482757647
8	0.6217104356535914	0.49480191029280207
9	0.47788645439684013	0.42787848908516257
>10	3.4746174639928755	7.01566246758198
>50	0.5171157226413986	3.661052301943423
>100	0.7178870132371279	17.80828174863958
>500	0.5008169462830738	35.98087774301641
>1k	0.09853351162078226	13.264045629850225
>5k	0.0014817069416658986	0.8873111884342979
>10k+	0.003704267354164747	6.323796600951175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	27364	2.0175432775714572	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	22669	1.6713816897846574	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	12626	0.930912930222819	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	12191	0.8988404508432113	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	10951	0.8074154521519159	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	6147	0.45321731206080057	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	5892	0.43441620345896165	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3923	0.289242153117703	No Hit
AGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCG	2997	0.22096832344984857	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	2971	0.21905134767083756	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	2890	0.21307923082084165	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCTG	2815	0.20754949299677136	No Hit
CACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACA	2697	0.19884937215356746	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	2556	0.18845346504431532	No Hit
CACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTA	2355	0.17363376767580696	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	2331	0.17186425157210447	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	2191	0.1615420743005066	No Hit
ACTTTTTTCAAGTCCTTTACCAACCAGTTGCTTGGACTTGCCCCTCTTC	2140	0.1577818525801388	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2128	0.15689709452828757	No Hit
ACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC	2080	0.1533580623208826	No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG	2023	0.14915546157458914	No Hit
CTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAG	1992	0.14686983660730676	No Hit
TCTCGACACTGTCATCCGGCCAAGATGCAGATCTTTGTCAAGACCCTG	1981	0.1460588083931098	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	1879	0.1385383649523742	No Hit
ATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCAT	1798	0.1325662481023783	No Hit
AGCCATTTGCTCTATTCCTAGACTAGCTAGGCCTGGTCAAGTCTTGGA	1778	0.1310916513492929	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1770	0.13050181264805874	No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGC	1703	0.1255619135252226	No Hit
ATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAAT	1697	0.12511953449929697	No Hit
TTTTTCAGCTCACTTCAAGGGTACCTGAAGCGAATTGGCACCAAAGCA	1682	0.12401358693448293	No Hit
AGTCTCCCAGCTCCAGCTCGGCCTTTGGGTTTGCTGTGGTGTCCTTGTCT	1653	0.12187542164250909	No Hit
ATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCA	1631	0.12025336521411514	No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG	1626	0.11988471602584379	No Hit
AGAGATCTCCAGTCAGAAAGAGACTGCCTGCGAGGGAGATTCCTGGCTG	1563	0.11523973625362474	No Hit
CTCTTCGTTAAGTCGGCCTTCCCAACATGGCGCAGTCTATTAACATCAC	1541	0.1136176798252308	No Hit
CTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCAT	1532	0.11295411128634236	No Hit
CTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCCAA	1477	0.1088989702153575	No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT	1461	0.10771929281288915	No Hit
ATCGCTCTTCTACTATGAACCCCCCTCCCCATACCCAACCCCCTGGTCAA	1452	0.10705572427400072	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1444	0.10646588557276657	No Hit
ACAGACGAGATCTCGATCGAAGGCGAGATGGCGGACGTGCTAGATCTTC	1426	0.10513874849498968	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	1421	0.10477009930671834	No Hit
TACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGAT	1414	0.10425399044313845	No Hit
AGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAAT	1369	0.10093614774869628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02838598749689413	0.0	0.0	0.0	0.0
2	0.04821931382589289	0.0	0.0	0.0	0.0
3	0.07181286187525944	0.0	0.0	0.0	0.0
4	0.0988717122943767	0.0	0.0	0.0	0.0
5	0.1256356433628769	0.0	0.0	0.0	0.0
6	0.17444479589000392	0.0	0.0	0.0	0.0
7	0.21602842432701247	0.0	0.0	0.0	0.0
8	0.27825640730721674	0.0	0.0	0.0	0.0
9	0.32654945097076393	0.0	0.0	0.0	0.0
10	0.3774967687898648	0.0	0.0	0.0	0.0
11	0.43766031631574953	0.0	0.0	0.0	0.0
12	0.49229412601756395	0.0	0.0	0.0	0.0
13	0.5478864236088838	0.0	0.0	0.0	0.0
14	0.6044372090897093	0.0	0.0	0.0	0.0
15	0.6688770871995416	0.0	0.0	0.0	0.0
16	0.7332432354717198	0.0	0.0	0.0	0.0
17	0.8084476698790757	0.0	0.0	0.0	0.0
18	0.8793757737024839	0.0	0.0	0.0	0.0
19	0.9600362160962558	0.0	0.0	0.0	0.0
20	1.0496916986838487	0.0	0.0	0.0	0.0
21	1.1213571008837995	0.0	0.0	0.0	0.0
22	1.1874927652596803	0.0	0.0	0.0	0.0
23	1.2428638733380373	0.0	0.0	0.0	0.0
24	1.2988248201176287	0.0	0.0	0.0	0.0
25	1.3567027426762308	0.0	0.0	0.0	0.0
26	1.4230595965650743	0.0	0.0	0.0	0.0
27	1.4740069143841752	0.0	0.0	0.0	0.0
28	1.522447417723031	0.0	0.0	0.0	0.0
29	1.6043612673569254	0.0	0.0	0.0	0.0
30	1.6608383230000965	0.0	0.0	0.0	0.0
31	1.7179789471821563	0.0	0.0	0.0	0.0
32	1.7730551359098963	0.0	0.0	0.0	0.0
33	1.8352093890524463	0.0	0.0	0.0	0.0
34	1.900828944564747	0.0	0.0	0.0	0.0
35	1.9650476331616165	0.0	0.0	0.0	0.0
36	2.027349345979475	0.0	0.0	0.0	0.0
37	2.0838264016226464	0.0	0.0	0.0	0.0
38	2.1419255136942112	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAG	60	0.0	82.93978	44
AAATCAT	30	3.159039E-8	82.93978	44
TCCGATG	110	0.0	82.93978	44
CGGTTCC	60	0.0	82.93978	44
GAGTACT	45	1.8189894E-12	82.93978	44
TGTGCAC	30	3.159039E-8	82.93978	44
GTGGACT	40	2.910383E-11	82.93978	44
AATGCGA	15	0.001134726	82.93978	44
ATATCCG	15	0.001134726	82.93978	44
TAGATCC	25	1.0361491E-6	82.93978	44
TGCGTCC	55	0.0	82.93978	44
AACCCGC	100	0.0	78.79279	44
GAAGTTC	100	0.0	78.79279	44
CGGATTA	90	0.0	78.332016	44
GCGTACA	85	0.0	78.060974	44
TGTTATG	95	0.0	74.209274	44
CCTCGAA	120	0.0	72.57231	44
GTATCAT	75	0.0	71.88115	44
GTACTGG	110	0.0	71.629814	44
TACTGTC	35	9.196265E-8	71.09124	44
>>END_MODULE