FastQCFastQC Report
Tue 24 May 2016
ERR840879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840906
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA139370.4905829337542319No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC95060.3346115640573817No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA90220.31757474552132314No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG82290.2896611151512933No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA65270.22975064996870717No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT63580.22380184349640575No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA43290.15238096579049076No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG40500.14256015510544875No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG36980.1301697416246789No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG35170.12379853469280575No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT34950.12302413385025762No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG34240.12052493113112507No Hit
CTTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAA32310.11373132373968023No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC30170.1061985155439849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGA800.073.1198144
TGCACTA950.073.1198144
CGGCTAT650.073.11980444
TTACGCG850.068.8186544
TAATCGT850.068.8186544
TCACTCG1050.066.1560244
ATAGCGA950.061.5745844
CCCGTCA1250.061.42064744
TACGCTT900.060.93317844
GCTAGTT750.058.49585344
CGATCTA304.8627044E-755.8873143
ATGTCGC900.055.88730643
GAGACAC2400.054.8398644
ATGCACC1600.052.55486744
CACGACG800.052.39435643
TAGCCGT2600.052.02755444
AGAACGG1000.051.1838744
CCGTACC203.8312442E-450.90247742
TGTCGTA1150.050.90247742
AGGTCGA2100.050.5647143
Produced by FastQC (version 0.11.5)